FastQCFastQC Report
Thu 26 May 2016
SRR938389_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938389_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4005851
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64370.1606899507745046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47900.11957509153485739No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51050.042.6614951
GTATCAA82500.040.7240331
ATCAACG102250.031.9755733
TCAACGC103900.031.5592584
TATCAAC104650.031.333082
CAACGCA106250.030.7717865
AACGCAG112800.029.0663056
GTACATG114000.025.2910061
TACATGG113450.024.5044292
ACGCAGA132450.024.4612797
CGCAGAG132450.024.4254138
ACATGGG116200.022.6976073
GCAGAGT145550.021.182079
GAGTACT73400.019.73768812-13
CATGGGG84300.019.504814
GGACCGA14650.019.1365746
GTGTAAG14800.018.9979131
AGAGTAC127600.018.96656810-11
TAAGGTG15000.018.6919235
CAGAGTA136050.018.64395110-11
Produced by FastQC (version 0.11.5)