##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938389_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4005851 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.020272596259822 31.0 31.0 34.0 28.0 34.0 2 31.170496606089444 31.0 31.0 34.0 27.0 34.0 3 31.23997073281058 31.0 31.0 34.0 27.0 34.0 4 34.895089957165155 37.0 35.0 37.0 32.0 37.0 5 34.88529902884556 37.0 35.0 37.0 32.0 37.0 6 34.88230840338295 37.0 35.0 37.0 32.0 37.0 7 34.78588619496831 37.0 35.0 37.0 32.0 37.0 8 34.73824238594995 37.0 35.0 37.0 31.0 37.0 9 36.2193176930445 39.0 35.0 39.0 32.0 39.0 10-11 36.06782703600309 39.0 35.0 39.0 30.0 39.0 12-13 35.8836374593064 38.0 35.0 39.0 30.0 39.0 14-15 36.86940265626455 39.0 36.0 41.0 30.5 41.0 16-17 36.67689587056533 39.0 36.0 41.0 30.0 41.0 18-19 36.49165720342569 39.0 36.0 40.0 29.5 41.0 20-21 36.459128659553244 39.0 36.0 40.5 29.5 41.0 22-23 36.56238549561628 39.0 36.0 41.0 30.0 41.0 24-25 36.469526075732716 39.0 36.0 41.0 29.5 41.0 26-27 36.26445591710725 39.0 35.5 41.0 29.0 41.0 28-29 36.064223806626856 39.0 35.0 40.0 27.5 41.0 30-31 35.81559174317766 38.5 35.0 40.0 27.0 41.0 32-33 35.53218629449772 38.0 34.0 40.0 27.0 41.0 34-35 35.26459508853425 38.0 34.0 40.0 26.0 41.0 36-37 34.974181266352645 38.0 34.0 40.0 25.0 41.0 38-39 34.647860342284325 38.0 33.0 40.0 24.5 41.0 40-41 34.55394783780026 38.0 33.0 40.0 24.0 41.0 42-43 34.32769279236796 38.0 33.0 40.0 24.0 41.0 44-45 34.03237851332963 38.0 33.0 40.0 23.0 41.0 46-47 33.66360967994066 38.0 32.5 40.0 21.5 41.0 48-49 33.290890000651544 37.0 32.0 40.0 19.0 41.0 50-51 32.50614076759221 36.5 30.5 39.5 17.5 40.5 52-53 32.51247649999962 37.0 31.0 39.0 17.0 40.0 54-55 32.561289473822164 37.0 31.0 39.5 14.5 41.0 56-57 32.18784148486801 36.0 31.0 39.0 11.0 41.0 58-59 31.70781202296341 36.0 30.0 39.0 8.0 41.0 60-61 31.177117546309134 35.0 29.0 39.0 6.5 40.0 62-63 30.543758367448014 35.0 28.0 38.5 2.0 40.0 64-65 30.003297676323957 34.5 28.0 38.0 2.0 40.0 66-67 29.393813199742077 34.0 27.5 38.0 2.0 40.0 68-69 28.72640395261831 34.0 26.0 37.0 2.0 39.5 70-71 28.044824682695385 33.5 26.0 36.5 2.0 39.0 72-73 27.36990167632296 33.0 24.5 36.0 2.0 39.0 74-75 26.616789541098758 32.0 22.5 35.0 2.0 38.0 76-77 25.82882176596184 32.0 20.5 35.0 2.0 37.0 78-79 24.99180636024655 31.0 17.0 35.0 2.0 36.5 80-81 24.182331170080964 31.0 9.0 34.5 2.0 36.0 82-83 23.411663713902488 30.0 4.5 34.0 2.0 35.5 84-85 22.638934523525712 29.5 2.0 34.0 2.0 35.0 86-87 21.874297496337235 29.0 2.0 34.0 2.0 35.0 88-89 21.07715676394354 29.0 2.0 34.0 2.0 35.0 90-91 20.236984850410064 27.0 2.0 34.0 2.0 35.0 92-93 19.338437700253955 25.5 2.0 33.5 2.0 35.0 94-95 18.42104112209865 24.5 2.0 33.0 2.0 35.0 96-97 17.338097198323155 21.0 2.0 32.5 2.0 34.5 98-99 16.084781735516373 4.5 2.0 32.0 2.0 34.0 100-101 13.404328193934322 2.0 2.0 28.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6945586429640933 1101 2 0.6324658513331514 1101 3 0.6503687269995915 1101 4 0.5747231363234704 1101 5 0.643169788257417 1101 6 0.5765528145982515 1101 7 0.5924091990062621 1101 8 0.5556836194546477 1101 9 0.7481467946727847 1101 10-11 0.7122781467045343 1101 12-13 0.8125589759541967 1101 14-15 1.0523466008021387 1101 16-17 1.1156552671413351 1101 18-19 1.1213366359506551 1101 20-21 1.0978002359848915 1101 22-23 1.086418721946707 1101 24-25 1.0935296014683686 1101 26-27 1.1862842946997816 1101 28-29 1.2772104305067629 1101 30-31 1.17583418943061 1101 32-33 1.2791591277024565 1101 34-35 1.4078291646068308 1101 36-37 1.4744780003434954 1101 38-39 1.51841511974839 1101 40-41 1.7102725473755314 1101 42-43 1.771033598905234 1101 44-45 1.8110708431721037 1101 46-47 1.9472556002801156 1101 48-49 2.0705204648340967 1101 50-51 2.180391547983831 1101 52-53 2.2963478919187637 1101 54-55 2.467948060949844 1101 56-57 2.502240975536445 1101 58-59 2.7827514948633763 1101 60-61 2.848020534659124 1101 62-63 2.9085882895445927 1101 64-65 3.0235805098860737 1101 66-67 3.1088023791798527 1101 68-69 3.325103915593022 1101 70-71 3.4791489311357786 1101 72-73 3.580015452769061 1101 74-75 3.869844317767093 1101 76-77 3.9286735022134707 1101 78-79 4.39637427148973 1101 80-81 4.872037485205841 1101 82-83 4.874548706317942 1101 84-85 5.122443639143352 1101 86-87 5.391113837754151 1101 88-89 5.763456749195004 1101 90-91 6.038464260330979 1101 92-93 6.058115832771978 1101 94-95 6.295630998486306 1101 96-97 6.276132489370696 1101 98-99 6.556255662778522 1101 100-101 6.368469118718764 1102 1 0.6396083738885601 1102 2 0.6035582499493337 1102 3 0.5757302972475244 1102 4 0.4370982083972734 1102 5 0.4988059596969734 1102 6 0.5277588495780705 1102 7 0.5484982341782825 1102 8 0.513652579669909 1102 9 0.6897610076268492 1102 10-11 0.7320834273118777 1102 12-13 0.7494853886007533 1102 14-15 0.9571491185803396 1102 16-17 1.0341367922230162 1102 18-19 1.119666926167838 1102 20-21 1.0078791567998024 1102 22-23 0.9193065991329448 1102 24-25 0.7290073306630589 1102 26-27 0.8451439670042262 1102 28-29 0.832313856414892 1102 30-31 0.9345941596401062 1102 32-33 1.0518924065800306 1102 34-35 1.1229664652026443 1102 36-37 1.2097989690154165 1102 38-39 1.380943348654796 1102 40-41 1.50456790582259 1102 42-43 1.7394802883151854 1102 44-45 1.9737233993896766 1102 46-47 1.9058492001263545 1102 48-49 1.8378902514956437 1102 50-51 1.9603442195194845 1102 52-53 2.124623045676209 1102 54-55 2.1894428716359826 1102 56-57 2.2673944927380703 1102 58-59 2.350236599610639 1102 60-61 2.3141308316031903 1102 62-63 2.714742791754855 1102 64-65 2.839044545058087 1102 66-67 3.0110943261335414 1102 68-69 3.1249759845916785 1102 70-71 3.253867242686809 1102 72-73 3.3962032290519737 1102 74-75 3.509463287592194 1102 76-77 3.6747875229707248 1102 78-79 4.08958912349819 1102 80-81 4.616607925336535 1102 82-83 4.785501522001201 1102 84-85 4.7221006879613405 1102 86-87 5.010972573099146 1102 88-89 5.339251362879413 1102 90-91 5.594859008553161 1102 92-93 5.519226009593044 1102 94-95 5.68203667790203 1102 96-97 5.781919415182728 1102 98-99 6.006101304715866 1102 100-101 5.9907062895817305 1103 1 0.4878349579105965 1103 2 0.4059546311163764 1103 3 0.40277919531915174 1103 4 0.3553144618407984 1103 5 0.2895021780791396 1103 6 0.46489884456930497 1103 7 0.45872394166638486 1103 8 0.4391941524222247 1103 9 0.6111468703866834 1103 10-11 0.47675770104090276 1103 12-13 0.5642745508847469 1103 14-15 0.5966432332810641 1103 16-17 0.7572577226011745 1103 18-19 0.8930985565209539 1103 20-21 0.8073446276237419 1103 22-23 0.8392328763681078 1103 24-25 0.8571104173147006 1103 26-27 0.981660214186796 1103 28-29 1.0025844862671391 1103 30-31 0.9137490870130023 1103 32-33 1.1516216202038692 1103 34-35 1.0931290935522568 1103 36-37 1.0877729234356508 1103 38-39 1.2777588695663908 1103 40-41 1.290341447019678 1103 42-43 1.3488161573359747 1103 44-45 1.2444236873936845 1103 46-47 1.637958516429883 1103 48-49 1.5070613008319853 1103 50-51 1.6572904379016506 1103 52-53 1.6989758192800224 1103 54-55 1.890028271085022 1103 56-57 2.0796362282752554 1103 58-59 2.2874392041686207 1103 60-61 2.5113157489585625 1103 62-63 2.619368856117486 1103 64-65 2.797733970302268 1103 66-67 2.7054031165167096 1103 68-69 2.977213493983111 1103 70-71 3.139303474843082 1103 72-73 3.221585147409101 1103 74-75 3.480975934724672 1103 76-77 3.6890390256003265 1103 78-79 3.7969167906657297 1103 80-81 4.138020397162229 1103 82-83 4.52091349295036 1103 84-85 4.750231948918014 1103 86-87 4.796519154616799 1103 88-89 4.958033450282368 1103 90-91 5.274638466354311 1103 92-93 5.33873045487228 1103 94-95 5.516144804623792 1103 96-97 5.753334234867179 1103 98-99 5.948715723699085 1103 100-101 5.703719688029011 1104 1 0.5965296070441184 1104 2 0.6114158508124206 1104 3 0.5937744591984249 1104 4 0.5278693886276997 1104 5 0.36233240190679794 1104 6 0.4681203689364608 1104 7 0.46144631666006575 1104 8 0.4936779226654693 1104 9 0.6324301135992485 1104 10-11 0.806286624193369 1104 12-13 0.6696990992631342 1104 14-15 0.8411708178841693 1104 16-17 0.9072850487470561 1104 18-19 1.0346808699043493 1104 20-21 1.028285738854109 1104 22-23 1.019065255445291 1104 24-25 1.040846148193495 1104 26-27 1.0052354918586772 1104 28-29 1.0535580804510403 1104 30-31 1.1190147057867463 1104 32-33 1.2411844820594027 1104 34-35 1.2932949317987408 1104 36-37 1.4308957405824074 1104 38-39 1.3444444733138354 1104 40-41 1.5862163709937747 1104 42-43 1.6329454340628544 1104 44-45 1.687454163140032 1104 46-47 1.6748701869764915 1104 48-49 1.7368846740010397 1104 50-51 1.8477996911638286 1104 52-53 1.9468250110306116 1104 54-55 2.180401325407921 1104 56-57 2.3590707436031906 1104 58-59 2.4582313478091464 1104 60-61 2.6285540748959484 1104 62-63 2.7639475153228226 1104 64-65 2.952559409895443 1104 66-67 3.1208661373943762 1104 68-69 3.376864114095401 1104 70-71 3.5366885847460026 1104 72-73 3.6868588900054426 1104 74-75 3.691723190698493 1104 76-77 3.798342005322226 1104 78-79 4.12425521172031 1104 80-81 4.4119993677499245 1104 82-83 4.684445054957273 1104 84-85 4.8249142439194905 1104 86-87 4.974296983798165 1104 88-89 5.381587015079877 1104 90-91 5.539554409155691 1104 92-93 5.473486228214544 1104 94-95 5.723992312392927 1104 96-97 6.06631477100246 1104 98-99 6.230369640220484 1104 100-101 5.855407771485403 1105 1 0.5950203312868609 1105 2 0.6354402367957199 1105 3 0.5765592825921999 1105 4 0.484310013300167 1105 5 0.4904793466714992 1105 6 0.4937955252792605 1105 7 0.5787331139908787 1105 8 0.4692989267530905 1105 9 0.7068545275321014 1105 10-11 0.6978484800435538 1105 12-13 0.8018751464978706 1105 14-15 0.9716174866470553 1105 16-17 1.0563260297763009 1105 18-19 1.0574405387021741 1105 20-21 0.9883764002103632 1105 22-23 1.001004604919487 1105 24-25 0.9430393145282565 1105 26-27 1.1256238159337286 1105 28-29 1.1813530355570805 1105 30-31 1.1322907425404054 1105 32-33 1.1544998228692478 1105 34-35 1.3163768938572318 1105 36-37 1.4486594271281916 1105 38-39 1.4067667316375605 1105 40-41 1.5129361418074652 1105 42-43 1.6562544862237019 1105 44-45 1.6955979635802336 1105 46-47 1.7585083451010775 1105 48-49 1.7849928387636425 1105 50-51 1.6776358369207358 1105 52-53 1.7703602798703528 1105 54-55 1.9018630869283726 1105 56-57 2.11808447382014 1105 58-59 2.106436462576994 1105 60-61 2.238353012813004 1105 62-63 2.4728800171570064 1105 64-65 2.7797525762302087 1105 66-67 2.8875677409998737 1105 68-69 2.8017566830085165 1105 70-71 3.0329180156232667 1105 72-73 3.270860392293809 1105 74-75 3.365164129803407 1105 76-77 3.5424149536505 1105 78-79 3.9039236601708573 1105 80-81 4.297591374178321 1105 82-83 4.513175446600357 1105 84-85 4.685068665189096 1105 86-87 5.049181691900042 1105 88-89 5.236646757245271 1105 90-91 5.408065746192339 1105 92-93 5.600345746783528 1105 94-95 5.815141912364979 1105 96-97 5.891275466946279 1105 98-99 6.116309269190197 1105 100-101 5.520136311470729 1106 1 0.3110993296353257 1106 2 0.3564429305338166 1106 3 0.24566129262646896 1106 4 0.30631467842113835 1106 5 0.28357722454638434 1106 6 0.09977788435844559 1106 7 0.30203404735648576 1106 8 0.3028127590470078 1106 9 0.3741463369402922 1106 10-11 0.19493525017951896 1106 12-13 0.20148573953174065 1106 14-15 0.5557579441279188 1106 16-17 0.3426170573517595 1106 18-19 0.5881361434222043 1106 20-21 0.550266250382748 1106 22-23 0.44501093433251526 1106 24-25 0.5509009651746268 1106 26-27 0.5619015733765451 1106 28-29 0.3127465755810377 1106 30-31 0.6848771246350438 1106 32-33 0.7451501923395654 1106 34-35 0.6799510356246472 1106 36-37 0.6662840320564811 1106 38-39 0.7756877395782595 1106 40-41 0.8493107343820441 1106 42-43 0.4184069897865186 1106 44-45 0.6628855143270371 1106 46-47 1.1423122964560264 1106 48-49 1.1846869878121211 1106 50-51 0.5527546347060124 1106 52-53 1.1092649126290937 1106 54-55 1.054267011437581 1106 56-57 1.0805733936636912 1106 58-59 0.48726465182471657 1106 60-61 1.7829348185537164 1106 62-63 2.1022338919728476 1106 64-65 2.314451322656396 1106 66-67 2.3732877485684263 1106 68-69 1.807437234169143 1106 70-71 2.203768143266309 1106 72-73 2.586907783109009 1106 74-75 2.8962600846321287 1106 76-77 3.0825045005415035 1106 78-79 3.4350010170479415 1106 80-81 3.901900905907052 1106 82-83 4.027884270061595 1106 84-85 4.096633338712387 1106 86-87 4.295685771140612 1106 88-89 4.432642303799536 1106 90-91 4.714285512167216 1106 92-93 5.124759017347426 1106 94-95 4.925486704013117 1106 96-97 5.171212104533659 1106 98-99 5.142481798164816 1106 100-101 4.700794657395868 1107 1 0.4847328243057305 1107 2 0.38016540972105517 1107 3 0.33907889457795193 1107 4 0.3737457136661675 1107 5 0.40294174316331066 1107 6 0.31955279844417817 1107 7 0.42402388992827866 1107 8 0.2731647040394165 1107 9 0.4323522462701206 1107 10-11 0.43149528981552976 1107 12-13 0.4899563672725975 1107 14-15 0.7882959607977753 1107 16-17 0.815988545954653 1107 18-19 0.7623241246175567 1107 20-21 0.7603467294394548 1107 22-23 0.7713641060199947 1107 24-25 0.7818441203494544 1107 26-27 0.9098133144905489 1107 28-29 0.9337606060813926 1107 30-31 1.0580982237903314 1107 32-33 1.1526922617348632 1107 34-35 1.19381047938721 1107 36-37 1.245994474473747 1107 38-39 1.3118469980031904 1107 40-41 1.2559189225014862 1107 42-43 1.4675407592001193 1107 44-45 1.6116250289964995 1107 46-47 1.49527283870016 1107 48-49 1.6129668181520458 1107 50-51 1.6958268953267819 1107 52-53 1.8552849081798684 1107 54-55 2.091541646569482 1107 56-57 2.2397661153197035 1107 58-59 2.364386630789845 1107 60-61 2.3771534039665703 1107 62-63 2.6541107073657706 1107 64-65 2.795717906373767 1107 66-67 2.9933547395934887 1107 68-69 3.1233431463762322 1107 70-71 3.045074731418879 1107 72-73 3.4408210667148786 1107 74-75 3.4047011573025827 1107 76-77 3.75825479861858 1107 78-79 3.793270366443398 1107 80-81 4.272094478810846 1107 82-83 4.514327289443795 1107 84-85 4.597041856944738 1107 86-87 4.848045931060103 1107 88-89 5.161343038623571 1107 90-91 5.352998285866537 1107 92-93 5.5191113076109914 1107 94-95 5.6933956275180115 1107 96-97 5.799837714810948 1107 98-99 5.683888020203643 1107 100-101 5.432060276178618 1108 1 0.256980834419025 1108 2 0.30548520156033376 1108 3 0.2689139899782411 1108 4 0.3220295684635275 1108 5 0.2899879849257374 1108 6 0.31689991434119946 1108 7 0.2942851552933945 1108 8 0.3734146740175248 1108 9 0.3780264887401046 1108 10-11 0.38253741282052545 1108 12-13 0.42030840385256596 1108 14-15 0.3701081290894237 1108 16-17 0.5404600991788229 1108 18-19 0.6402046048425802 1108 20-21 0.5372439955353769 1108 22-23 0.5159984617524529 1108 24-25 0.5132164875078971 1108 26-27 0.485858763943412 1108 28-29 0.634551819875945 1108 30-31 0.6774302082071202 1108 32-33 0.6987192306733263 1108 34-35 0.8548094938641313 1108 36-37 0.9916958093241703 1108 38-39 1.0926164730416374 1108 40-41 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2311 52-53 0.955164208778676 2311 54-55 0.8312106146408453 2311 56-57 0.9760658174948134 2311 58-59 1.1048593093385861 2311 60-61 1.0611778140467827 2311 62-63 1.3427124695803876 2311 64-65 1.4555502614084652 2311 66-67 1.321030341006857 2311 68-69 1.3624845691739864 2311 70-71 1.561454008156744 2311 72-73 1.6888234689404662 2311 74-75 1.722429266232151 2311 76-77 1.832730933307218 2311 78-79 2.188400180052472 2311 80-81 2.163301267820298 2311 82-83 2.323255751649743 2311 84-85 2.568334370590847 2311 86-87 2.7205848092731806 2311 88-89 2.834055933681803 2311 90-91 3.0530191053790077 2311 92-93 3.2667304224439597 2311 94-95 3.1376622631318476 2311 96-97 3.0701500982368017 2311 98-99 3.355223029852201 2311 100-101 3.2605274055459255 2312 1 -0.423245758729216 2312 2 -0.3217596121473427 2312 3 -0.20224400622323913 2312 4 -0.25872855663929784 2312 5 -0.23156062853650639 2312 6 -0.18295017873901287 2312 7 -0.24489659483349868 2312 8 -0.3004450619251884 2312 9 -0.37213154566328654 2312 10-11 -0.27960977092704553 2312 12-13 -0.30926712039202187 2312 14-15 -0.13495868648806209 2312 16-17 -0.12879023158512837 2312 18-19 -0.01979177856404135 2312 20-21 -0.08208459441243576 2312 22-23 -0.21940260739748396 2312 24-25 -0.2651355959574744 2312 26-27 -0.08350326088476123 2312 28-29 -0.13178732288415063 2312 30-31 -0.015061113446499519 2312 32-33 0.09841007433239213 2312 34-35 0.047230924631818993 2312 36-37 0.17980020540068153 2312 38-39 0.300387354407853 2312 40-41 0.48765562832364395 2312 42-43 0.40394893001073484 2312 44-45 0.3384907416249803 2312 46-47 0.3980587654982912 2312 48-49 0.612666253257494 2312 50-51 0.6419961413258406 2312 52-53 0.7756080849314415 2312 54-55 0.9296553850140583 2312 56-57 1.0347438517495746 2312 58-59 1.1774913606598716 2312 60-61 1.2398451459502802 2312 62-63 1.3991811082037984 2312 64-65 1.3437399716882794 2312 66-67 1.421254998263482 2312 68-69 1.5594469010429428 2312 70-71 1.75210835182099 2312 72-73 1.8834066414753288 2312 74-75 1.8545538721319694 2312 76-77 1.9810902625300315 2312 78-79 2.142177817237826 2312 80-81 2.3074749018722613 2312 82-83 2.5861025211550484 2312 84-85 2.7827931385735454 2312 86-87 3.010378770093954 2312 88-89 3.1527011699839953 2312 90-91 3.2041031255808896 2312 92-93 3.326175436605741 2312 94-95 3.50578235055357 2312 96-97 3.4682598813965697 2312 98-99 3.3921585614280865 2312 100-101 3.0448481319503315 2313 1 -0.4336411510101925 2313 2 -0.4897393981079219 2313 3 -0.5181049527745074 2313 4 -0.29876585325860816 2313 5 -0.2348796765363943 2313 6 -0.27682894261877067 2313 7 -0.21957800328804922 2313 8 -0.26885282269103783 2313 9 -0.4169788447469287 2313 10-11 -0.38356044263682065 2313 12-13 -0.3678343866265479 2313 14-15 -0.3471599347804286 2313 16-17 -0.2614729872955195 2313 18-19 -0.19009788913655257 2313 20-21 -0.22919654474083018 2313 22-23 -0.34836236593178427 2313 24-25 -0.2134203481033623 2313 26-27 -0.24898864036291002 2313 28-29 -0.10086611069201012 2313 30-31 -0.10649748844397067 2313 32-33 -0.2734567593107684 2313 34-35 -0.032544242295912795 2313 36-37 -0.04949711484761821 2313 38-39 -2.9241547387925948E-5 2313 40-41 0.08939538947182513 2313 42-43 0.12598670649240518 2313 44-45 0.19216376939392177 2313 46-47 0.10831536831986455 2313 48-49 0.24409798568415653 2313 50-51 0.5630955203007133 2313 52-53 0.5023872656672452 2313 54-55 0.6038343684731089 2313 56-57 0.7054547242732951 2313 58-59 0.82350736517499 2313 60-61 0.9021727319890225 2313 62-63 1.0550090951031699 2313 64-65 1.1308624945939059 2313 66-67 1.24754579875583 2313 68-69 1.533962665952 2313 70-71 1.6000737484129246 2313 72-73 1.6821825698106814 2313 74-75 1.8296566231477733 2313 76-77 2.012289606328004 2313 78-79 2.098619579308025 2313 80-81 2.2134251301319914 2313 82-83 2.375517882342507 2313 84-85 2.6390681842352457 2313 86-87 2.8300449666515597 2313 88-89 2.7705785788052673 2313 90-91 2.8215279812808127 2313 92-93 2.8569575017174706 2313 94-95 2.993707765117591 2313 96-97 3.2864890917498926 2313 98-99 3.2834183691840373 2313 100-101 3.115975210297293 2314 1 -0.484405563629565 2314 2 -0.5063505946392013 2314 3 -0.5580720014562424 2314 4 -0.47480534053936907 2314 5 -0.35616981157926375 2314 6 -0.3900978062626521 2314 7 -0.23577111326910938 2314 8 -0.33435407242559734 2314 9 -0.46907799908190384 2314 10-11 -0.3933100313648481 2314 12-13 -0.41799281347045536 2314 14-15 -0.383008526575928 2314 16-17 -0.4369043070580929 2314 18-19 -0.487359715825157 2314 20-21 -0.29855516948227034 2314 22-23 -0.42532037767355035 2314 24-25 -0.42846163798844117 2314 26-27 -0.3077531264612219 2314 28-29 -0.2716519345573829 2314 30-31 -0.32556743360735396 2314 32-33 -0.21806381990428036 2314 34-35 -0.1950984047324198 2314 36-37 -0.10766213346354192 2314 38-39 0.08911401879075953 2314 40-41 -0.01957360941855768 2314 42-43 0.11237440960861278 2314 44-45 0.19869251328868387 2314 46-47 0.29192270160427825 2314 48-49 0.27311898870186013 2314 50-51 0.30020338706163585 2314 52-53 0.4997742651310304 2314 54-55 0.4900280150012719 2314 56-57 0.6993749076129419 2314 58-59 0.9686178750229502 2314 60-61 0.8935591578430042 2314 62-63 0.9809170313781799 2314 64-65 1.0468357711006533 2314 66-67 1.3235548332177736 2314 68-69 1.5489311448873018 2314 70-71 1.49068588185456 2314 72-73 1.476329433525887 2314 74-75 1.651419312890706 2314 76-77 1.8259488187608994 2314 78-79 2.0144971554209476 2314 80-81 2.2448876379337435 2314 82-83 2.515808841707038 2314 84-85 2.7740929014948534 2314 86-87 2.8380926344440063 2314 88-89 3.0157752432492515 2314 90-91 3.1302674985457237 2314 92-93 3.1159012462624425 2314 94-95 3.2944940759694923 2314 96-97 3.3601732773258064 2314 98-99 3.5868894356662118 2314 100-101 3.4910326763232504 2315 1 -0.5522730433024421 2315 2 -0.601635374334883 2315 3 -0.5211790822679241 2315 4 -0.5482827328799331 2315 5 -0.4970082924876422 2315 6 -0.5020841944353478 2315 7 -0.5252547856763101 2315 8 -0.6041164931965071 2315 9 -0.6877965511838582 2315 10-11 -0.5358179314315592 2315 12-13 -0.5768198145322501 2315 14-15 -0.7146949422448827 2315 16-17 -0.48168554954042975 2315 18-19 -0.4563039188562854 2315 20-21 -0.41671227885436224 2315 22-23 -0.5309794030765218 2315 24-25 -0.5487427199864001 2315 26-27 -0.6178887363007917 2315 28-29 -0.6085312776054863 2315 30-31 -0.5472072744085708 2315 32-33 -0.4280812270313348 2315 34-35 -0.5636033287974058 2315 36-37 -0.371950214859325 2315 38-39 -0.3004315309332739 2315 40-41 -0.42713105863487044 2315 42-43 -0.27306011179643264 2315 44-45 -0.1818781919590151 2315 46-47 -0.2712600153995197 2315 48-49 -0.03280836701645029 2315 50-51 -0.002631742055733355 2315 52-53 0.19948880071631692 2315 54-55 0.26488237515659563 2315 56-57 0.503108717017696 2315 58-59 0.6883609258772552 2315 60-61 0.7493395408255985 2315 62-63 0.7809400828688347 2315 64-65 0.9180265243625314 2315 66-67 1.2434004629367728 2315 68-69 1.2878607054653735 2315 70-71 1.4056611653250144 2315 72-73 1.5846689135650927 2315 74-75 1.760950279281861 2315 76-77 1.8076546742157475 2315 78-79 2.098289243336719 2315 80-81 2.2680077617292262 2315 82-83 2.434055432139502 2315 84-85 2.6533518108503067 2315 86-87 2.82450945355113 2315 88-89 2.9985490507793564 2315 90-91 3.0255150002886957 2315 92-93 3.1372461795458726 2315 94-95 3.2305605328419738 2315 96-97 3.321081840355319 2315 98-99 3.264005367949327 2315 100-101 3.1024834357809183 2316 1 -1.0113091949416884 2316 2 -0.8872534757607156 2316 3 -0.8192396373456141 2316 4 -0.6530224962569235 2316 5 -0.6444004263323819 2316 6 -0.5835838437024208 2316 7 -0.5230198775611257 2316 8 -0.701776229957467 2316 9 -0.8442861022276702 2316 10-11 -0.7833925545965243 2316 12-13 -0.7336451089585765 2316 14-15 -1.2645213339513575 2316 16-17 -0.9690311008222707 2316 18-19 -0.9579896233786656 2316 20-21 -0.6417991957045075 2316 22-23 -1.0318766347207387 2316 24-25 -1.0664630731356866 2316 26-27 -0.8237682915461946 2316 28-29 -0.7388953951920882 2316 30-31 -0.6403153722819965 2316 32-33 -0.6389489213725454 2316 34-35 -0.7237505554826811 2316 36-37 -0.6814339341792106 2316 38-39 -0.800899249979075 2316 40-41 -0.7772791734228193 2316 42-43 -0.6583584962610374 2316 44-45 -0.504593964842762 2316 46-47 -0.32334565915223834 2316 48-49 -0.042889233948919525 2316 50-51 -0.004692410738435626 2316 52-53 -0.04088948593978614 2316 54-55 -0.05620539733764218 2316 56-57 0.2593842668749673 2316 58-59 0.34417921556281783 2316 60-61 0.14872855323739387 2316 62-63 0.3371880880189302 2316 64-65 0.47670982424549635 2316 66-67 0.598279978510579 2316 68-69 0.7961562380835332 2316 70-71 0.5705757451758124 2316 72-73 0.9068731257864648 2316 74-75 1.1428415277288053 2316 76-77 1.2919363839892029 2316 78-79 1.4080727536594502 2316 80-81 1.7475817384275736 2316 82-83 2.011252855108996 2316 84-85 2.051711841361371 2316 86-87 2.4181655152801262 2316 88-89 2.1824619274566075 2316 90-91 2.34008615038616 2316 92-93 2.6230800403310433 2316 94-95 2.6970024238785406 2316 96-97 2.5325514834732914 2316 98-99 2.5546849783743006 2316 100-101 2.5672683616581544 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13769.0 3 5351.0 4 5959.0 5 6865.0 6 8013.0 7 9968.0 8 12602.0 9 16227.0 10 20038.0 11 24240.0 12 28752.0 13 33027.0 14 36933.0 15 41983.0 16 47042.0 17 53068.0 18 54523.0 19 56945.0 20 62204.0 21 68153.0 22 75307.0 23 83296.0 24 93921.0 25 103787.0 26 115978.0 27 128552.0 28 142245.0 29 156919.0 30 175062.0 31 196676.0 32 223144.0 33 254835.0 34 295416.0 35 345380.0 36 396220.0 37 396454.0 38 203605.0 39 13392.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.39537796263501 17.91706111714384 13.910929848198014 24.776631072023132 2 15.832586883848299 19.221538299413687 39.60014613512305 25.345728681614965 3 19.72579168909073 22.601482672806803 28.416671765409546 29.256053872692924 4 13.389939632600992 15.473204287154221 35.29769628894836 35.839159791296424 5 14.815360617841625 36.22138991886083 33.56129398056507 15.401955482732479 6 34.60885689686653 34.15751727741551 16.727390620078694 14.506235205639273 7 30.637974952849 30.867431087623075 19.68650733503018 18.808086624497744 8 26.907423629638583 34.915386543953346 19.099082946358205 19.078106880049862 9 26.623877547299905 16.688206071040327 19.160929693136534 37.52698668852323 10-11 25.810231110047898 25.61782876765863 26.78158201421405 21.790358108079424 12-13 26.35417708442193 22.680147725798562 27.14922895188923 23.816446237890275 14-15 23.250548438845463 24.407142321175726 24.815979118934603 27.526330121044207 16-17 23.518433694914926 27.543159019371448 25.496140054408894 23.44226723130473 18-19 23.491760767171463 26.50975612709801 27.06169340083575 22.936789704894775 20-21 23.824000067924906 25.817321451135772 27.28678445977971 23.071894021159608 22-23 23.61456749245256 25.518257434445285 27.15849246005289 23.708682613049266 24-25 23.565195162580014 25.80477660319367 27.145193656521506 23.484834577704813 26-27 23.26555270378705 26.08288465222146 27.139527734149354 23.512034909842132 28-29 23.317247963336943 26.305448390509902 27.1436623151096 23.233641331043557 30-31 23.39741744841146 25.980780633947155 27.002181697588725 23.619620220052663 32-33 23.241932326230877 25.878983894617825 27.166832641466463 23.712251137684838 34-35 23.791536438223307 26.041352059343843 26.83515198475577 23.331959517677088 36-37 23.856509778157665 25.686189021824656 26.86491916604802 23.59238203396966 38-39 23.29101421079597 25.984875874005397 26.965332409183834 23.758777506014795 40-41 23.673742404139478 25.88513715618572 26.937871366358547 23.50324907331625 42-43 23.564953815308673 25.75391717840811 26.810740765246443 23.870388241036775 44-45 23.436019415839628 26.032466669204844 26.965498000557986 23.566015914397543 46-47 23.609653449988947 26.271369360599195 26.480448629081415 23.638528560330442 48-49 23.69162213476255 25.970592028086966 26.676174129887976 23.661611707262512 50-51 23.690177856800833 26.24252855140651 26.50929378052927 23.557999811263382 52-53 23.691701587120097 26.149591396056298 26.456095843584993 23.702611173238612 54-55 23.43988456118145 26.09630054037051 26.70599924854921 23.757815649898827 56-57 23.477272585426746 26.335128725230145 26.62181307536277 23.56578561398034 58-59 23.563437912706554 25.958415406546386 26.710867616843547 23.767279063903516 60-61 23.524896017203957 26.12001900843334 26.748403262889703 23.606681711473 62-63 23.288122818950196 26.220330014827738 26.796326256154245 23.69522091006782 64-65 23.26842008620342 26.089797705694295 26.729838569246407 23.911943638855877 66-67 23.318969789125262 26.164259664187146 26.578090506555213 23.93868004013238 68-69 23.317779991362027 25.940927258792136 26.828491325828786 23.912801424017054 70-71 23.386175725222724 25.994082647446653 26.638322828395154 23.981418798935472 72-73 23.436169739991204 25.956863436334523 26.626097351376643 23.980869472297638 74-75 23.3112162987561 26.338540800005596 26.37869874042379 23.971544160814513 76-77 23.599073068274336 26.124330384950788 26.38779489962858 23.888801647146295 78-79 23.636883363291393 26.029745855801877 26.489714399840224 23.84365638106651 80-81 23.515531019035222 26.268499807204932 26.320789774484073 23.89517939927578 82-83 23.68312743588172 26.227785501472216 26.21296782567023 23.876119236975832 84-85 23.67213587035282 26.099207086751875 26.271350508897196 23.957306533998107 86-87 23.747835166199945 25.970678710891793 26.337722186059313 23.94376393684895 88-89 23.74583672535377 26.076459063988374 26.200538037589727 23.977166173068134 90-91 23.749199393224337 26.085747638756235 26.194842406876788 23.970210561142636 92-93 23.81249722245663 25.737603168571543 26.317928724117667 24.131970884854162 94-95 23.721874803761732 25.798507946221942 26.323449603765674 24.156167646250644 96-97 23.8696053830693 25.716602374442903 26.180367776487774 24.233424466000024 98-99 23.89133527115937 25.718342790481973 25.99721996655721 24.39310197180145 100-101 24.467915762148245 25.177019888451547 26.103745712101627 24.25131863729859 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 316.0 1 239.5 2 206.5 3 278.5 4 262.5 5 250.5 6 483.0 7 1025.5 8 1605.5 9 1831.0 10 1646.0 11 1491.0 12 1584.5 13 1733.0 14 1932.0 15 2125.5 16 2257.0 17 2423.0 18 2644.0 19 2924.0 20 3270.0 21 3884.5 22 4702.5 23 5816.5 24 7407.0 25 9436.0 26 12272.0 27 15986.0 28 19500.0 29 23536.5 30 28906.5 31 35786.0 32 44726.5 33 51769.5 34 56967.5 35 63201.0 36 72412.0 37 81796.5 38 88213.0 39 94111.5 40 101127.0 41 111280.5 42 120675.0 43 127770.0 44 135270.0 45 144126.5 46 150847.5 47 156702.0 48 160351.0 49 160906.5 50 161691.0 51 159774.5 52 159321.5 53 161117.5 54 161958.0 55 153854.0 56 138363.0 57 125730.5 58 115372.0 59 102942.5 60 87022.5 61 73269.5 62 61354.5 63 49063.0 64 39017.0 65 31001.5 66 24377.0 67 19343.0 68 15014.5 69 11337.5 70 8814.0 71 6672.5 72 5147.5 73 4075.5 74 3069.0 75 2417.5 76 1782.0 77 1139.0 78 732.5 79 469.5 80 277.5 81 174.0 82 104.0 83 57.5 84 35.5 85 20.0 86 20.0 87 16.0 88 12.5 89 9.5 90 7.0 91 5.0 92 3.0 93 3.5 94 2.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29701554051810714 2 0.033401142478839074 3 0.039841721521843926 4 0.042512814380764534 5 0.055818351706041984 6 0.05526915504346018 7 0.029057496147510232 8 0.03212800476103579 9 0.038992963043308404 10-11 0.03050537825795318 12-13 0.03169114377943663 14-15 0.026823264270188784 16-17 0.020158013865218653 18-19 0.023902536564640073 20-21 0.03567281958315474 22-23 0.02948187538677799 24-25 0.05048864773053217 26-27 0.042637631804078584 28-29 0.034549462773328314 30-31 0.03496136027026467 32-33 0.030630195681267226 34-35 0.029694065006411873 36-37 0.02895764220885899 38-39 0.018148453349862488 40-41 0.017699110625931918 42-43 0.018373124711827775 44-45 0.008949409251617196 46-47 0.016762979951076562 48-49 0.014391448908109662 50-51 0.006715177374295749 52-53 0.004980215190230491 54-55 0.006640286920307321 56-57 0.005641747533794941 58-59 0.007014739190249464 60-61 0.006502987754661868 62-63 0.00455583595096273 64-65 0.008125614257744483 66-67 0.0026960563435834235 68-69 0.007014739190249464 70-71 0.012793785889689857 72-73 0.013105829447974976 74-75 0.010397291362060147 76-77 0.009436197202541982 78-79 0.006265834650365179 80-81 0.007551454110499867 82-83 0.012469260589073335 84-85 0.004480945496974301 86-87 0.0014603638527743543 88-89 0.01848546039281042 90-91 0.026860709497182996 92-93 0.012806267632021261 94-95 0.020832027951114506 96-97 0.01749940274862944 98-99 0.01256911452772457 100-101 0.10439729285986923 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4005851.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.801610201682465 #Duplication Level Percentage of deduplicated Percentage of total 1 73.46167160865829 35.850478626097456 2 13.855010076394384 13.522936021771631 3 5.118005692583782 7.493007564583968 4 2.4353540234394297 4.753967910199605 5 1.3580973561648062 3.3138668895745202 6 0.8267843795748412 2.4209045407710765 7 0.5520124761407359 1.8857368380960013 8 0.3771411825866547 1.472407758687638 9 0.29038301509643194 1.2754042840732809 >10 1.5152967710518945 13.511926061702436 >50 0.12264797640057863 4.141718692671049 >100 0.08150379384247058 7.376030005361844 >500 0.004439675752185697 1.486385980243978 >1k 0.0016003481787285345 1.3330588522140334 >5k 5.162413479769454E-5 0.16216997395161342 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6437 0.1606899507745046 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4790 0.11957509153485739 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 2.4963484662809477E-5 8 0.0 0.0 0.0 0.0 2.4963484662809477E-5 9 0.0 0.0 0.0 0.0 2.4963484662809477E-5 10-11 0.0 0.0 0.0 0.0 2.4963484662809477E-5 12-13 0.0 0.0 0.0 0.0 2.4963484662809477E-5 14-15 0.0 0.0 0.0 0.0 8.737219631983317E-5 16-17 0.0 0.0 0.0 4.9926969325618954E-5 9.985393865123791E-5 18-19 0.0 0.0 0.0 6.240871165702369E-5 9.985393865123791E-5 20-21 0.0 0.0 0.0 9.985393865123791E-5 9.985393865123791E-5 22-23 0.0 0.0 0.0 1.8722613497107108E-4 1.3729916564545212E-4 24-25 0.0 0.0 0.0 4.1189749693635637E-4 1.7474439263966634E-4 26-27 0.0 0.0 0.0 9.735759018495696E-4 1.7474439263966634E-4 28-29 0.0 2.4963484662809477E-5 0.0 0.0028708007362230896 1.7474439263966634E-4 30-31 0.0 2.4963484662809477E-5 0.0 0.009873058184141147 1.7474439263966634E-4 32-33 0.0 2.4963484662809477E-5 0.0 0.02511326557078633 1.7474439263966634E-4 34-35 0.0 2.4963484662809477E-5 0.0 0.04791740881026279 1.9970787730247581E-4 36-37 0.0 2.4963484662809477E-5 0.0 0.08963139168181741 1.9970787730247581E-4 38-39 0.0 4.9926969325618954E-5 0.0 0.15347550370695265 2.1218961963388055E-4 40-41 0.0 4.9926969325618954E-5 0.0 0.22467136196528525 2.4963484662809477E-4 42-43 0.0 4.9926969325618954E-5 0.0 0.29632904468987986 2.7459833129090424E-4 44-45 0.0 4.9926969325618954E-5 0.0 0.38688408530422125 2.87080073622309E-4 46-47 0.0 4.9926969325618954E-5 0.0 0.49647378297395484 3.1204355828511846E-4 48-49 0.0 4.9926969325618954E-5 0.0 0.6131531102879264 3.245253006165232E-4 50-51 0.0 4.9926969325618954E-5 0.0 0.7415028666817612 3.245253006165232E-4 52-53 0.0 4.9926969325618954E-5 0.0 0.8667197057504136 3.3700704294792794E-4 54-55 0.0 4.9926969325618954E-5 0.0 1.0076635401566358 3.619705276107374E-4 56-57 0.0 4.9926969325618954E-5 0.0 1.1714988899986545 3.7445226994214215E-4 58-59 0.0 4.9926969325618954E-5 0.0 1.3506368559389752 3.7445226994214215E-4 60-61 0.0 4.9926969325618954E-5 0.0 1.5435297019285041 3.869340122735469E-4 62-63 0.0 4.9926969325618954E-5 0.0 1.7344379508873395 4.493427239305706E-4 64-65 0.0 6.240871165702369E-5 0.0 1.9360430530241888 4.618244662619753E-4 66-67 0.0 7.489045398842843E-5 0.0 2.168116088191997 4.7430620859338006E-4 68-69 0.0 7.489045398842843E-5 0.0 2.423093120537933 4.7430620859338006E-4 70-71 0.0 7.489045398842843E-5 0.0 2.698665027730687 4.7430620859338006E-4 72-73 0.0 7.489045398842843E-5 0.0 2.9757472257455406 5.24233177918999E-4 74-75 0.0 9.985393865123791E-5 0.0 3.2660226254046894 5.491966625818085E-4 76-77 0.0 1.2481742331404738E-4 0.0 3.582497202217456 5.491966625818085E-4 78-79 0.0 1.2481742331404738E-4 0.0 3.9320359144661143 5.491966625818085E-4 80-81 0.0 1.2481742331404738E-4 0.0 4.298687095451129 5.491966625818085E-4 82-83 0.0 1.2481742331404738E-4 0.0 4.6744624300804 5.74160147244618E-4 84-85 0.0 1.4978090797685686E-4 0.0 5.051136450157532 5.74160147244618E-4 86-87 0.0 1.4978090797685686E-4 0.0 5.445272427756299 5.991236319074274E-4 88-89 0.0 1.4978090797685686E-4 0.0 5.866556194925872 5.991236319074274E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5105 0.0 42.661495 1 GTATCAA 8250 0.0 40.724033 1 ATCAACG 10225 0.0 31.975573 3 TCAACGC 10390 0.0 31.559258 4 TATCAAC 10465 0.0 31.33308 2 CAACGCA 10625 0.0 30.771786 5 AACGCAG 11280 0.0 29.066305 6 GTACATG 11400 0.0 25.291006 1 TACATGG 11345 0.0 24.504429 2 ACGCAGA 13245 0.0 24.461279 7 CGCAGAG 13245 0.0 24.425413 8 ACATGGG 11620 0.0 22.697607 3 GCAGAGT 14555 0.0 21.18207 9 GAGTACT 7340 0.0 19.737688 12-13 CATGGGG 8430 0.0 19.50481 4 GGACCGA 1465 0.0 19.136574 6 GTGTAAG 1480 0.0 18.997913 1 AGAGTAC 12760 0.0 18.966568 10-11 TAAGGTG 1500 0.0 18.691923 5 CAGAGTA 13605 0.0 18.643951 10-11 >>END_MODULE