FastQCFastQC Report
Thu 26 May 2016
SRR938389_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938389_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4005851
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66350.16563272073774085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52940.13215668780491335No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51800.043.3375361
GTATCAA83250.039.0201071
ATCAACG104700.030.786573
TCAACGC106450.030.280454
CAACGCA108300.029.7192975
TATCAAC108800.029.681982
AACGCAG113000.028.5804776
TACATGG113950.024.7927152
GTACATG118800.024.5011771
CGCAGAG135950.023.6509448
ACATGGG117650.023.1952083
ACGCAGA139050.023.1919787
GCAGAGT156750.019.9974849
GGACCGA14850.019.8291576
AGGACCG17750.019.2846815
GAGTACT76950.019.07167812-13
ACCGAGT15200.019.0601028
AGAGTAC135350.018.720410-11
CATGGGG84750.018.6802584
GTGTAAG15850.018.6043471
Produced by FastQC (version 0.11.5)