##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938389_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4005851 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.800673315108327 31.0 31.0 34.0 30.0 34.0 2 32.03958809251767 31.0 31.0 34.0 30.0 34.0 3 32.1170510336006 33.0 31.0 34.0 30.0 34.0 4 35.7757402858968 37.0 35.0 37.0 35.0 37.0 5 35.640265701345356 37.0 35.0 37.0 33.0 37.0 6 35.67508801500605 37.0 35.0 37.0 33.0 37.0 7 35.609356164270714 37.0 35.0 37.0 33.0 37.0 8 35.591992063608956 37.0 35.0 37.0 33.0 37.0 9 37.17195647067253 39.0 37.0 39.0 33.0 39.0 10-11 37.10304140119041 39.0 37.0 39.0 33.0 39.0 12-13 36.987122087166995 39.0 37.0 39.0 33.0 39.0 14-15 38.060971688662406 40.0 37.0 41.0 33.0 41.0 16-17 37.96771447565074 40.0 37.0 41.0 33.0 41.0 18-19 37.93482882913019 40.0 37.0 41.0 33.0 41.0 20-21 37.916069893762895 40.0 37.0 41.0 32.5 41.0 22-23 37.95728560548058 40.0 37.0 41.0 33.0 41.0 24-25 37.88609960779869 40.0 37.0 41.0 32.0 41.0 26-27 37.70852373191114 40.0 37.0 41.0 32.0 41.0 28-29 37.57774789926036 39.5 37.0 41.0 32.0 41.0 30-31 37.363248408390625 39.0 36.0 41.0 31.5 41.0 32-33 37.17342095350027 39.0 36.0 41.0 31.0 41.0 34-35 37.01932722909564 39.0 36.0 40.5 31.0 41.0 36-37 37.05852052410337 39.0 36.0 41.0 31.0 41.0 38-39 36.926829654922265 39.0 36.0 41.0 30.0 41.0 40-41 36.76688274226875 39.0 35.0 41.0 30.0 41.0 42-43 36.5591583411365 39.0 35.0 40.0 30.0 41.0 44-45 36.306791241112066 39.0 35.0 40.0 30.0 41.0 46-47 35.99034325041046 38.0 35.0 40.0 29.0 41.0 48-49 35.635078164415006 38.0 34.5 40.0 28.0 41.0 50-51 35.27103803910829 38.0 34.0 40.0 27.0 41.0 52-53 34.74906193465509 38.0 33.0 40.0 26.0 41.0 54-55 34.41821575490451 37.5 33.0 40.0 25.5 41.0 56-57 34.03463820796131 37.0 33.0 40.0 24.0 41.0 58-59 33.558184141147535 36.5 32.0 39.0 23.0 41.0 60-61 33.123204532569986 36.0 31.0 39.0 22.0 40.0 62-63 32.61826862756503 35.5 31.0 39.0 21.5 40.0 64-65 32.10325608715851 35.0 31.0 38.5 19.5 40.0 66-67 31.581682893347754 35.0 30.0 38.0 18.0 40.0 68-69 30.987709228326267 34.0 29.0 37.5 15.5 39.5 70-71 30.399837263043484 34.0 29.0 37.0 11.5 39.0 72-73 29.793134592375004 34.0 28.0 36.0 8.0 39.0 74-75 29.15328628049321 33.5 27.5 35.5 7.0 38.0 76-77 27.872327752579913 31.5 26.0 34.5 3.5 36.0 78-79 28.479352452200544 33.0 27.0 35.0 2.0 37.0 80-81 28.15595737335213 33.0 26.0 35.0 2.0 36.5 82-83 27.646734863578303 33.0 26.0 35.0 2.0 36.0 84-85 27.03995280902859 32.0 25.5 35.0 2.0 35.5 86-87 26.371021663062358 32.0 24.5 34.5 2.0 35.0 88-89 25.602711259105746 31.0 22.0 34.0 2.0 35.0 90-91 25.035106772568426 31.0 19.5 34.0 2.0 35.0 92-93 24.214901153337955 31.0 16.5 34.0 2.0 35.0 94-95 23.394286881863554 30.5 4.5 34.0 2.0 35.0 96-97 22.377377740709775 30.0 2.0 34.0 2.0 35.0 98-99 21.161252502901384 29.0 2.0 34.0 2.0 35.0 100-101 18.013422241616077 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3746918986004921 1101 2 0.3579234036124035 1101 3 0.32730195269154194 1101 4 0.22330649103785305 1101 5 0.1966660926908972 1101 6 0.23911008049245197 1101 7 0.26232971929103144 1101 8 0.3166606681063939 1101 9 0.3396566736800324 1101 10-11 0.32889139133340706 1101 12-13 0.3838016860892637 1101 14-15 0.547201698823848 1101 16-17 0.5455801775283078 1101 18-19 0.6223945421414356 1101 20-21 0.6547116085494196 1101 22-23 0.6026049462488174 1101 24-25 0.5733303503771339 1101 26-27 0.5781003423543041 1101 28-29 0.5703753578524626 1101 30-31 0.6739149096072623 1101 32-33 0.5813336334117878 1101 34-35 0.41133397396867366 1101 36-37 0.6715345700688644 1101 38-39 0.8551260914912362 1101 40-41 0.96309706839385 1101 42-43 0.787653557740164 1101 44-45 0.8163372681198311 1101 46-47 0.9035090040833467 1101 48-49 0.9779073797775624 1101 50-51 1.1667476340611884 1101 52-53 1.1700324828514184 1101 54-55 1.2231382065645136 1101 56-57 1.4061178383833024 1101 58-59 1.4163139894791215 1101 60-61 1.6015541781509341 1101 62-63 1.7100606058681933 1101 64-65 1.6340431454142959 1101 66-67 1.6337762267818725 1101 68-69 1.7889438010946002 1101 70-71 1.9142169330648606 1101 72-73 1.92765054690652 1101 74-75 2.0815209197172564 1101 76-77 1.980498294629566 1101 78-79 2.4089877207655803 1101 80-81 2.657140687437039 1101 82-83 2.8199604655520503 1101 84-85 2.9370419450000433 1101 86-87 2.9814798832961316 1101 88-89 3.0215070792298704 1101 90-91 3.3117410992708862 1101 92-93 3.6984115575724488 1101 94-95 3.8048030216651156 1101 96-97 4.211007855928056 1101 98-99 4.448274698358283 1101 100-101 4.7358155850277015 1102 1 0.3341684025405236 1102 2 0.3237664268682181 1102 3 0.34035932343918773 1102 4 0.22367887235632367 1102 5 0.14875503176450877 1102 6 0.15612310182629585 1102 7 0.24881588112074127 1102 8 0.23677286299395917 1102 9 0.4052738656846486 1102 10-11 0.4004126290808614 1102 12-13 0.35590612103064245 1102 14-15 0.5020895039362827 1102 16-17 0.4688516074725726 1102 18-19 0.5665944027987493 1102 20-21 0.49954571867818487 1102 22-23 0.47655386994668447 1102 24-25 0.3997225760161882 1102 26-27 0.5295976756235774 1102 28-29 0.4930401666166446 1102 30-31 0.39031650484078284 1102 32-33 0.2879542128851682 1102 34-35 0.4971528525002853 1102 36-37 0.6696966880776998 1102 38-39 0.7160747028692853 1102 40-41 0.6406839173271592 1102 42-43 0.6943540190125006 1102 44-45 0.4964587124788551 1102 46-47 0.5136798189978151 1102 48-49 0.5366925758183072 1102 50-51 0.7040067874342384 1102 52-53 0.7873566227707016 1102 54-55 0.680077112003687 1102 56-57 0.8735284668668726 1102 58-59 0.9268367648010454 1102 60-61 0.9918338245088023 1102 62-63 1.1720116437309542 1102 64-65 1.132154211145604 1102 66-67 1.034080137986944 1102 68-69 1.2076205260609676 1102 70-71 1.423229161586864 1102 72-73 1.4267976735642165 1102 74-75 1.5685085950968478 1102 76-77 1.5355416590347168 1102 78-79 1.7519839489031916 1102 80-81 1.9463368242811683 1102 82-83 2.0019528257288712 1102 84-85 2.1317973745599126 1102 86-87 2.168087969976895 1102 88-89 2.3858311470753435 1102 90-91 2.5573235757267767 1102 92-93 2.754421912175559 1102 94-95 2.834494425662811 1102 96-97 3.4009433498738524 1102 98-99 3.871705291285437 1102 100-101 4.260281638266218 1103 1 0.19793781150621115 1103 2 0.21999889618953006 1103 3 0.1956529623072072 1103 4 0.14583952850458104 1103 5 0.13390873825085947 1103 6 0.15251522554279973 1103 7 0.1960516687490923 1103 8 0.20978031353240567 1103 9 0.27076503773573535 1103 10-11 0.32398200048081094 1103 12-13 0.34305159435896826 1103 14-15 0.4722795239713449 1103 16-17 0.4701742345699884 1103 18-19 0.40145775741933676 1103 20-21 0.4042343926075631 1103 22-23 0.3997397577503392 1103 24-25 0.32439544102996365 1103 26-27 0.3616983831116727 1103 28-29 0.3854456261708563 1103 30-31 0.4402476343173731 1103 32-33 0.4246739411596465 1103 34-35 0.4961706649220261 1103 36-37 0.48589065251547225 1103 38-39 0.612960659543468 1103 40-41 0.5929715143719889 1103 42-43 0.6458159523959282 1103 44-45 0.6957253979283919 1103 46-47 0.7719208032202047 1103 48-49 0.889446614135359 1103 50-51 0.9264390125782356 1103 52-53 1.0591760567802169 1103 54-55 1.0537192670249738 1103 56-57 1.1356121132480368 1103 58-59 1.3000054349738548 1103 60-61 1.367600791701392 1103 62-63 1.386566802759262 1103 64-65 1.3307478437526612 1103 66-67 1.3035438870478053 1103 68-69 1.4405437667321443 1103 70-71 1.5769708658192698 1103 72-73 1.624017808800879 1103 74-75 1.7696066103735788 1103 76-77 1.6855893988293538 1103 78-79 2.044194536180928 1103 80-81 2.2195962783257457 1103 82-83 2.371247528959522 1103 84-85 2.472706465468999 1103 86-87 2.597867427778045 1103 88-89 2.812143600118169 1103 90-91 3.1465461214317614 1103 92-93 3.2009337766884585 1103 94-95 3.255821591953822 1103 96-97 3.6043735715117187 1103 98-99 3.925357776885239 1103 100-101 4.340449275381172 1104 1 0.2500884347027217 1104 2 0.24423496694882374 1104 3 0.16703002734343642 1104 4 0.1477521736631644 1104 5 0.11204560091323401 1104 6 0.13444746163576582 1104 7 0.16737377307205037 1104 8 0.2363822857029163 1104 9 0.2687074344669611 1104 10-11 0.25451483565171884 1104 12-13 0.3213710733980264 1104 14-15 0.3697606454906577 1104 16-17 0.49288852275164885 1104 18-19 0.4314013532933956 1104 20-21 0.4525356069247408 1104 22-23 0.4038609476652084 1104 24-25 0.3521207460279925 1104 26-27 0.410647806918611 1104 28-29 0.4614693080081622 1104 30-31 0.5144890407688081 1104 32-33 0.39819891528308204 1104 34-35 0.4783688837261124 1104 36-37 0.6233592089424178 1104 38-39 0.6392272850050205 1104 40-41 0.7243210188619855 1104 42-43 0.6847241895942702 1104 44-45 0.6951179000780954 1104 46-47 0.6865096341669741 1104 48-49 0.7317813526370998 1104 50-51 0.6929338576211066 1104 52-53 0.8420740839928627 1104 54-55 0.9020281918799213 1104 56-57 0.9746877308004329 1104 58-59 1.2166257767295576 1104 60-61 1.232731210821548 1104 62-63 1.2507797604105164 1104 64-65 1.368356145966267 1104 66-67 1.5255238236116675 1104 68-69 1.5463948003759889 1104 70-71 1.5711669898244551 1104 72-73 1.7566545721515219 1104 74-75 1.8242215831276205 1104 76-77 1.5914345416223483 1104 78-79 1.9752227888250253 1104 80-81 2.184049033701843 1104 82-83 2.411754407376062 1104 84-85 2.5632389737131085 1104 86-87 2.7905059614681633 1104 88-89 2.703462213109624 1104 90-91 2.9883843938271717 1104 92-93 3.2488501426441516 1104 94-95 3.3441289623643584 1104 96-97 3.7301657830049884 1104 98-99 4.241927907618948 1104 100-101 4.4136934008766495 1105 1 0.24333163073388064 1105 2 0.23070563738868088 1105 3 0.2212364347780138 1105 4 0.18028021055657462 1105 5 0.13337809576002968 1105 6 0.19914892549350327 1105 7 0.2153736333242975 1105 8 0.2290105916107521 1105 9 0.26868821915060437 1105 10-11 0.3310485672661514 1105 12-13 0.35184219801808325 1105 14-15 0.4513925499031899 1105 16-17 0.45710174288930006 1105 18-19 0.4238706961295762 1105 20-21 0.36061128033423273 1105 22-23 0.3605967894707476 1105 24-25 0.4081648364447972 1105 26-27 0.47118042055100773 1105 28-29 0.5302461657798858 1105 30-31 0.5528698883389467 1105 32-33 0.5531861633004169 1105 34-35 0.6025959039546009 1105 36-37 0.6908006022275401 1105 38-39 0.6211832295251654 1105 40-41 0.6223907529918833 1105 42-43 0.6696873311238107 1105 44-45 0.8000413881820592 1105 46-47 0.9143858176557771 1105 48-49 0.8505686914904729 1105 50-51 0.8012689378013746 1105 52-53 0.8910067605117504 1105 54-55 0.7952678942172042 1105 56-57 0.9446323557523968 1105 58-59 1.0760954339981836 1105 60-61 1.083590659516652 1105 62-63 1.3014426888247286 1105 64-65 1.4144204998075836 1105 66-67 1.423187415827723 1105 68-69 1.4117260025560547 1105 70-71 1.3907701061993976 1105 72-73 1.5078415546481168 1105 74-75 1.752993899097298 1105 76-77 1.6552521009278003 1105 78-79 2.0039357119986967 1105 80-81 2.137930527605345 1105 82-83 2.267397596419837 1105 84-85 2.430085565614725 1105 86-87 2.65176264315771 1105 88-89 2.7099412684147985 1105 90-91 2.8417636692350605 1105 92-93 3.112788234388553 1105 94-95 3.2664222230288544 1105 96-97 3.635960947732652 1105 98-99 4.096548317778613 1105 100-101 4.382720057760974 1106 1 0.174337363035864 1106 2 0.14073270055289555 1106 3 0.11544481700958897 1106 4 0.08299387631346633 1106 5 0.09459103439991168 1106 6 0.12695616949449828 1106 7 0.10711756235092196 1106 8 0.014940261884341055 1106 9 0.16403619760717447 1106 10-11 0.274140093087027 1106 12-13 0.14317821197211344 1106 14-15 0.051225725378550635 1106 16-17 0.008422745511161622 1106 18-19 0.2655772405780539 1106 20-21 0.41405217990727294 1106 22-23 0.30179042577279347 1106 24-25 0.12179465526840261 1106 26-27 0.09696627385287826 1106 28-29 0.2720232207232627 1106 30-31 0.3520417494045205 1106 32-33 0.31056968486837633 1106 34-35 0.2767944714990378 1106 36-37 0.3341969188268479 1106 38-39 0.35330677958123147 1106 40-41 0.40131447605383386 1106 42-43 0.3966907842333498 1106 44-45 -0.09889985828320391 1106 46-47 -0.253763563966821 1106 48-49 0.2082826815884502 1106 50-51 0.2526650330221756 1106 52-53 0.530365075476233 1106 54-55 0.5814391581717331 1106 56-57 0.4754932755980903 1106 58-59 0.8846778585924824 1106 60-61 0.5746136888121143 1106 62-63 0.781885999303249 1106 64-65 0.3859624892205886 1106 66-67 0.7622038159866342 1106 68-69 0.6403656261838293 1106 70-71 0.8678320035965648 1106 72-73 1.2420809586223989 1106 74-75 1.5514315453764773 1106 76-77 1.5329865613509135 1106 78-79 1.7264398983347213 1106 80-81 1.7940441594726373 1106 82-83 2.042710980889453 1106 84-85 2.11424374056449 1106 86-87 2.306739014774614 1106 88-89 2.555277018942771 1106 90-91 2.652181810485697 1106 92-93 2.2324683891522596 1106 94-95 2.7389021960896756 1106 96-97 3.1724058351737163 1106 98-99 3.5687032309890654 1106 100-101 4.041460762603574 1107 1 0.16336728443053516 1107 2 0.18794817992890955 1107 3 0.21625276554453166 1107 4 0.19762302211123028 1107 5 0.1287374391938414 1107 6 0.08969092054487504 1107 7 0.11500528887371075 1107 8 0.12187379087132655 1107 9 0.1424595575867329 1107 10-11 0.20912771034412714 1107 12-13 0.223281645946372 1107 14-15 0.34813058352613524 1107 16-17 0.31754341286697496 1107 18-19 0.2619559286459179 1107 20-21 0.2754783567941672 1107 22-23 0.36638841295425095 1107 24-25 0.38729948801189806 1107 26-27 0.30704955999213723 1107 28-29 0.2515572204579968 1107 30-31 0.21916437399863753 1107 32-33 0.38937308873667575 1107 34-35 0.36885177879106834 1107 36-37 0.4403139376771108 1107 38-39 0.5761026408187249 1107 40-41 0.5708860345530766 1107 42-43 0.6239950832100973 1107 44-45 0.7119855867832428 1107 46-47 0.5962482257798811 1107 48-49 0.7715900248462262 1107 50-51 0.7279453377590528 1107 52-53 0.7933467796139411 1107 54-55 0.8915632698178229 1107 56-57 0.86426350676836 1107 58-59 0.8673935109549546 1107 60-61 0.9617601981698769 1107 62-63 1.08410133130743 1107 64-65 1.0120690060732613 1107 66-67 1.3136207084480098 1107 68-69 1.3379826587186123 1107 70-71 1.3622549446190568 1107 72-73 1.5176692704920782 1107 74-75 1.5706646223529965 1107 76-77 1.6073099491907712 1107 78-79 1.777277215970848 1107 80-81 2.13147223675443 1107 82-83 2.1131533139399714 1107 84-85 2.4533319440106034 1107 86-87 2.7092051771489025 1107 88-89 2.896642117222239 1107 90-91 3.0143484431164858 1107 92-93 3.352033175564859 1107 94-95 3.46311765160549 1107 96-97 3.792115712665616 1107 98-99 4.103988521806329 1107 100-101 4.41029123376536 1108 1 0.08294976434829593 1108 2 0.07459189354623419 1108 3 0.08748110477826287 1108 4 0.0797595945438232 1108 5 -0.015654214429794422 1108 6 -0.045022223363190506 1108 7 0.012288324526295469 1108 8 -0.014064939425196599 1108 9 0.01369505287637196 1108 10-11 0.07931316624191709 1108 12-13 0.1308346281544175 1108 14-15 0.2847792784292622 1108 16-17 0.2065380585717591 1108 18-19 0.28669815968499535 1108 20-21 0.26191257128397893 1108 22-23 0.20385604577791838 1108 24-25 0.12050386139074476 1108 26-27 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0.5736922573607117 2316 74-75 0.855015762061548 2316 76-77 0.850372835633241 2316 78-79 0.9504606920121113 2316 80-81 1.0960738274870714 2316 82-83 1.403116073034461 2316 84-85 1.5780401048935495 2316 86-87 1.6904635902143568 2316 88-89 1.9479981218469824 2316 90-91 2.0856493473988387 2316 92-93 2.0588444771393952 2316 94-95 2.7439223874980705 2316 96-97 2.7181341474025693 2316 98-99 2.933775102233838 2316 100-101 3.248924798335814 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 26.0 4 52.0 5 133.0 6 242.0 7 631.0 8 1561.0 9 2767.0 10 4370.0 11 6812.0 12 9848.0 13 13742.0 14 18246.0 15 23750.0 16 29365.0 17 35811.0 18 36720.0 19 35957.0 20 38362.0 21 41588.0 22 46260.0 23 51763.0 24 59756.0 25 69958.0 26 81894.0 27 97807.0 28 115828.0 29 137623.0 30 161184.0 31 189917.0 32 226624.0 33 273798.0 34 336865.0 35 421994.0 36 531065.0 37 587237.0 38 301319.0 39 14968.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.256100453087356 18.733627070409526 14.558108765929829 24.452163710573284 2 16.413638023274807 19.976334600183108 38.6276965094784 24.982330867063688 3 19.84301431683668 22.891810787392316 28.903204643816544 28.361970251954453 4 13.932819767884528 16.20085220343942 34.897578566951196 34.96874946172486 5 14.70266247755072 36.39920445779511 33.684175542049964 15.21395752260421 6 34.57669793509544 34.702938277035265 16.39129862793199 14.3290651599373 7 30.551735448972018 30.960462583356197 19.631608864133987 18.856193103537798 8 26.836045574336143 35.41237554766765 18.86151032577098 18.890068552225234 9 26.295036934723733 16.698649051100503 19.147791567884077 37.85852244629169 10-11 25.81548839435116 25.83198925771328 26.742432506850605 21.610089841084953 12-13 26.277125634478164 22.797215872482525 27.18201450827802 23.743643984761288 14-15 23.090599225982196 24.518423176498576 24.810758063642407 27.58021953387682 16-17 23.377529518696527 27.689422297534282 25.586797911355163 23.346250272414025 18-19 23.355673987874237 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23.81721415949043 44-45 23.40900753455068 26.00502802661127 27.101515285602822 23.484449153235236 46-47 23.588045879912098 26.25167598678832 26.592427722562682 23.567850410736902 48-49 23.57615148441617 26.002290150082967 26.802544577918653 23.61901378758221 50-51 23.69670015185288 26.21208327518922 26.61823917065313 23.472977402304778 52-53 23.686003298674862 26.15552850068562 26.49841444427164 23.660053756367873 54-55 23.44577968576465 26.07568279499163 26.753878264568502 23.724659254675224 56-57 23.4750119263048 26.3006287552882 26.712663551390204 23.511695767016796 58-59 23.532028525274654 25.97275834772686 26.75400308199182 23.741210045006667 60-61 23.483898926844756 26.148189236194757 26.738962582482472 23.628949254478012 62-63 23.24107911152961 26.26737739371734 26.78902685097374 23.702516643779312 64-65 23.172017631209947 26.117571522255822 26.70445056493614 24.005960281598092 66-67 23.197689875669177 26.196745006359457 26.62741568882471 23.978149429146658 68-69 23.222252206215874 26.02171429170554 26.793025484536205 23.963008017542382 70-71 23.241378673345565 26.034530490524983 26.639071198604242 24.08501963752521 72-73 23.27767558004529 26.030436479040286 26.606731004223573 24.085156936690854 74-75 23.158574794719026 26.38894956402522 26.26193535406085 24.190540287194906 76-77 23.329275102843315 26.12749450740929 26.51122071190366 24.032009677843735 78-79 23.33535371135871 26.062938936071262 26.63844711148767 23.963260241082356 80-81 23.189184911659154 26.346830933175696 26.479187427980293 23.984796727184857 82-83 23.264155601716205 26.28398900898369 26.424534620528096 24.02732076877201 84-85 23.20913142880538 26.174793705541276 26.418235396619107 24.19783946903424 86-87 23.258395677364042 26.056478405730292 26.488639650067096 24.19648626683857 88-89 23.167617359611565 26.199158730356853 26.305752836476653 24.327471073554925 90-91 23.183889542729137 26.155315746174846 26.439227618911033 24.221567092184983 92-93 23.11171456295217 25.8786103199751 26.52165848736458 24.488016629708152 94-95 22.95186465996863 25.959140766843298 26.505479110431217 24.583515462756854 96-97 22.996362071380087 25.877922069492847 26.42110253227092 24.704613326856144 98-99 22.935263942667863 25.89970270986115 26.31685502031903 24.84817832715196 100-101 23.026443728099093 25.596150308038762 26.456010207130763 24.921395756731382 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 289.0 1 215.5 2 197.5 3 282.5 4 254.5 5 243.5 6 462.0 7 1038.0 8 1619.5 9 1833.0 10 1665.0 11 1526.5 12 1598.0 13 1699.5 14 1914.5 15 2074.5 16 2245.0 17 2483.0 18 2698.5 19 2939.5 20 3315.0 21 3856.5 22 4519.0 23 5621.0 24 7292.5 25 9402.5 26 12342.5 27 15897.5 28 19314.0 29 23378.0 30 28695.0 31 35470.5 32 45025.5 33 52203.5 34 57157.5 35 63843.0 36 72881.0 37 82038.0 38 88693.5 39 94948.0 40 101786.0 41 112250.5 42 122185.5 43 129810.5 44 137797.5 45 146602.0 46 153162.5 47 160397.0 48 164001.5 49 163115.5 50 164480.5 51 161790.5 52 160399.5 53 163024.5 54 165461.0 55 156776.5 56 139469.5 57 125299.5 58 113723.0 59 100406.5 60 83754.0 61 70216.0 62 58527.0 63 46031.5 64 35666.5 65 27738.5 66 21058.0 67 16582.5 68 12924.5 69 9576.0 70 7397.5 71 5511.5 72 4213.0 73 3379.5 74 2632.5 75 2107.0 76 1478.0 77 853.5 78 519.5 79 342.0 80 193.5 81 88.5 82 42.0 83 21.0 84 11.0 85 6.0 86 4.5 87 2.0 88 2.0 89 2.0 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.4963484662809477E-5 2 0.04226317953413644 3 1.9970787730247581E-4 4 0.0 5 0.10115203985370398 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001135838552157831 26-27 0.011682910822194834 28-29 0.0 30-31 0.0 32-33 4.1189749693635637E-4 34-35 0.014378967165778257 36-37 0.003033063386531351 38-39 0.011308458552252692 40-41 0.006353206846685011 42-43 0.005304740490847014 44-45 0.00260868414726359 46-47 4.9926969325618954E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.4978090797685686E-4 68-69 2.4963484662809477E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 7.489045398842843E-5 82-83 8.487584785355221E-4 84-85 0.004218828908014801 86-87 0.0013605099141231164 88-89 2.4963484662809477E-5 90-91 7.61386282215689E-4 92-93 1.9970787730247581E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 5.991236319074274E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4005851.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.827461592689204 #Duplication Level Percentage of deduplicated Percentage of total 1 70.98203546855842 32.52926504206264 2 15.002484503495635 13.750515647577224 3 5.513468769827519 7.580048348752859 4 2.7350506890035318 5.0136172161746995 5 1.5069602025702906 3.4530080402500567 6 0.9256999221197737 2.545348657637959 7 0.6004754853830113 1.9262787070618947 8 0.4283243610723432 1.570321456500476 9 0.32410342693657673 1.3367553614995429 >10 1.7389111654613776 14.452959128548256 >50 0.1424164686141209 4.5293043831214534 >100 0.09247288117442114 7.847653521015293 >500 0.0056838288248188444 1.792521888573171 >1k 0.0018035225605380074 1.3736322620205013 >5k 1.0930439760836389E-4 0.29877033920398466 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6635 0.16563272073774085 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5294 0.13215668780491335 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 2.4963484662809477E-5 7 0.0 0.0 0.0 0.0 4.9926969325618954E-5 8 0.0 0.0 0.0 0.0 4.9926969325618954E-5 9 0.0 0.0 0.0 0.0 4.9926969325618954E-5 10-11 0.0 0.0 0.0 0.0 4.9926969325618954E-5 12-13 0.0 0.0 0.0 0.0 4.9926969325618954E-5 14-15 0.0 0.0 0.0 0.0 4.9926969325618954E-5 16-17 0.0 0.0 0.0 4.9926969325618954E-5 4.9926969325618954E-5 18-19 0.0 0.0 0.0 6.240871165702369E-5 4.9926969325618954E-5 20-21 0.0 0.0 0.0 9.985393865123791E-5 4.9926969325618954E-5 22-23 0.0 0.0 0.0 1.8722613497107108E-4 7.489045398842843E-5 24-25 0.0 0.0 0.0 4.1189749693635637E-4 9.985393865123791E-5 26-27 0.0 0.0 0.0 9.735759018495696E-4 9.985393865123791E-5 28-29 0.0 0.0 0.0 0.0028832824785544947 1.1233568098264265E-4 30-31 0.0 0.0 0.0 0.009947948638129575 1.2481742331404738E-4 32-33 0.0 0.0 0.0 0.025312973448088808 1.9970787730247581E-4 34-35 0.0 0.0 0.0 0.048079671460571044 1.9970787730247581E-4 36-37 0.0 0.0 0.0 0.09000584395175956 1.9970787730247581E-4 38-39 0.0 0.0 0.0 0.15446156135113365 1.9970787730247581E-4 40-41 0.0 0.0 0.0 0.22605683536407123 1.9970787730247581E-4 42-43 0.0 0.0 0.0 0.29808897035860793 2.1218961963388055E-4 44-45 0.0 0.0 0.0 0.38893109104657164 2.246713619652853E-4 46-47 0.0 0.0 0.0 0.49910743060588125 2.4963484662809477E-4 48-49 0.0 0.0 0.0 0.616348436324766 2.7459833129090424E-4 50-51 0.0 0.0 0.0 0.7444984848412983 2.7459833129090424E-4 52-53 0.0 0.0 0.0 0.869540579517311 2.87080073622309E-4 54-55 0.0 0.0 0.0 1.0103096695308937 2.995618159537137E-4 56-57 0.0 0.0 0.0 1.1741949463422379 2.995618159537137E-4 58-59 0.0 0.0 0.0 1.3535326201598612 2.995618159537137E-4 60-61 0.0 0.0 0.0 1.5463755391800644 3.619705276107374E-4 62-63 0.0 0.0 0.0 1.737471014273871 4.243792392677611E-4 64-65 0.0 0.0 0.0 1.9407861151101227 4.243792392677611E-4 66-67 0.0 0.0 0.0 2.175230681320898 4.3686098159916584E-4 68-69 0.0 0.0 0.0 2.4321673472128644 4.493427239305706E-4 70-71 0.0 0.0 0.0 2.710073340221591 4.493427239305706E-4 72-73 0.0 0.0 0.0 2.990063784199662 4.7430620859338006E-4 74-75 0.0 0.0 0.0 3.2866299819938387 4.7430620859338006E-4 76-77 0.0 0.0 0.0 3.6102191519355062 4.7430620859338006E-4 78-79 0.0 0.0 0.0 3.9682704124541828 4.867879509247848E-4 80-81 0.0 0.0 0.0 4.345356330028251 4.992696932561895E-4 82-83 0.0 0.0 0.0 4.734387275013473 5.74160147244618E-4 84-85 0.0 0.0 0.0 5.125764787557001 5.74160147244618E-4 86-87 0.0 0.0 0.0 5.536701190333839 5.866418895760227E-4 88-89 0.0 0.0 0.0 5.980302312791963 5.991236319074274E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5180 0.0 43.337536 1 GTATCAA 8325 0.0 39.020107 1 ATCAACG 10470 0.0 30.78657 3 TCAACGC 10645 0.0 30.28045 4 CAACGCA 10830 0.0 29.719297 5 TATCAAC 10880 0.0 29.68198 2 AACGCAG 11300 0.0 28.580477 6 TACATGG 11395 0.0 24.792715 2 GTACATG 11880 0.0 24.501177 1 CGCAGAG 13595 0.0 23.650944 8 ACATGGG 11765 0.0 23.195208 3 ACGCAGA 13905 0.0 23.191978 7 GCAGAGT 15675 0.0 19.997484 9 GGACCGA 1485 0.0 19.829157 6 AGGACCG 1775 0.0 19.284681 5 GAGTACT 7695 0.0 19.071678 12-13 ACCGAGT 1520 0.0 19.060102 8 AGAGTAC 13535 0.0 18.7204 10-11 CATGGGG 8475 0.0 18.680258 4 GTGTAAG 1585 0.0 18.604347 1 >>END_MODULE