FastQCFastQC Report
Thu 26 May 2016
SRR938388_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938388_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3986648
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58730.147316743289099No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45160.11327812237247933No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55100.039.7473141
GTATCAA86550.037.763321
ATCAACG107750.029.992463
TATCAAC108300.029.8401452
TCAACGC109000.029.7357124
CAACGCA111900.028.7098985
AACGCAG119300.026.8818056
GTACATG114550.026.2417131
TACATGG112600.025.57732
ACATGGG119750.023.2167173
ACGCAGA140150.022.8786017
CGCAGAG139150.022.8381928
CATGGGG88300.020.6138214
GCAGAGT149600.020.5117829
TAAGGTG15650.020.0421665
GTACCGT2702.296838E-819.3563736
GAGTACT73950.019.27041212-13
AGAGTAC129750.018.72569710-11
GTAAGGT14200.018.4075014
GTACTTT78700.018.01769614-15
Produced by FastQC (version 0.11.5)