##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938388_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3986648 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.984042232973668 31.0 31.0 34.0 28.0 34.0 2 31.162499423074223 31.0 31.0 34.0 27.0 34.0 3 31.225081070613708 31.0 31.0 34.0 27.0 34.0 4 34.8812837250743 37.0 35.0 37.0 32.0 37.0 5 34.860140147813404 37.0 35.0 37.0 32.0 37.0 6 34.82601373384357 37.0 35.0 37.0 32.0 37.0 7 34.7310901288501 37.0 35.0 37.0 31.0 37.0 8 34.68417728377324 37.0 35.0 37.0 30.0 37.0 9 36.151316343956125 39.0 35.0 39.0 31.0 39.0 10-11 35.97197946746239 38.0 35.0 39.0 30.0 39.0 12-13 35.76887199471837 38.0 35.0 39.0 30.0 39.0 14-15 36.93398514240535 39.0 36.0 41.0 31.0 41.0 16-17 36.84533899155381 39.0 36.0 41.0 30.5 41.0 18-19 36.80367930652518 39.0 36.0 41.0 30.0 41.0 20-21 36.71475924134762 39.0 36.0 41.0 30.0 41.0 22-23 36.54331696703596 39.0 36.0 41.0 30.0 41.0 24-25 36.391911199584214 39.0 36.0 41.0 29.0 41.0 26-27 36.159890213532776 39.0 35.0 40.5 28.5 41.0 28-29 35.91456632238412 39.0 35.0 40.0 27.0 41.0 30-31 35.651472113916256 38.0 34.5 40.0 27.0 41.0 32-33 35.36567060849114 38.0 34.0 40.0 26.5 41.0 34-35 35.052568975239346 38.0 34.0 40.0 25.0 41.0 36-37 34.84166171681072 38.0 34.0 40.0 25.0 41.0 38-39 34.59623109439308 38.0 33.0 40.0 24.0 41.0 40-41 34.30726991698289 38.0 33.0 40.0 24.0 41.0 42-43 33.883953637241106 38.0 33.0 40.0 22.5 41.0 44-45 33.42124611954705 37.5 32.0 40.0 20.0 41.0 46-47 32.94996021720503 37.0 31.0 40.0 17.0 41.0 48-49 32.45500129432044 37.0 31.0 39.5 14.5 41.0 50-51 31.729325864736488 36.0 30.0 38.5 12.5 40.0 52-53 31.709051187865093 36.0 30.0 39.0 9.0 40.0 54-55 31.638727572637464 36.0 30.0 39.0 8.0 40.0 56-57 31.157717837140375 36.0 29.0 39.0 4.5 40.0 58-59 30.647592037220242 35.0 28.5 39.0 2.0 40.0 60-61 30.050835564113008 35.0 28.0 38.5 2.0 40.0 62-63 29.26938934663908 34.0 26.5 38.0 2.0 40.0 64-65 28.55967343994253 34.0 26.0 38.0 2.0 40.0 66-67 27.804832907244382 33.5 24.5 37.0 2.0 39.0 68-69 26.897384218521424 32.5 22.0 36.0 2.0 39.0 70-71 26.00695709779243 32.0 19.0 36.0 2.0 39.0 72-73 25.052810029879737 31.0 14.5 35.0 2.0 38.0 74-75 24.05435543845356 30.5 7.5 35.0 2.0 37.0 76-77 23.04701669171695 29.5 2.0 34.0 2.0 36.5 78-79 22.02084094206461 29.0 2.0 34.0 2.0 36.0 80-81 21.01703348276547 27.5 2.0 34.0 2.0 35.0 82-83 19.970152368606406 26.0 2.0 33.0 2.0 35.0 84-85 18.95349915517999 25.5 2.0 32.5 2.0 35.0 86-87 17.928672784755513 23.0 2.0 32.0 2.0 34.0 88-89 16.824899765416962 19.0 2.0 31.5 2.0 34.0 90-91 15.655431706034744 9.0 2.0 31.0 2.0 34.0 92-93 14.488864956223875 2.0 2.0 30.5 2.0 34.0 94-95 13.252175010183993 2.0 2.0 29.5 2.0 34.0 96-97 11.881541711232092 2.0 2.0 29.0 2.0 34.0 98-99 10.277540680792486 2.0 2.0 25.0 2.0 33.0 100-101 7.258290548852068 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5408529038038736 1101 2 0.4688117659311537 1101 3 0.47575174933032827 1101 4 0.3124288119512144 1101 5 0.31436841234930313 1101 6 0.4668196173916712 1101 7 0.421919620968616 1101 8 0.3967245693638617 1101 9 0.5129513363847238 1101 10-11 0.5348753573095095 1101 12-13 0.5358799528954776 1101 14-15 0.6775196109101742 1101 16-17 0.6877359677451835 1101 18-19 0.6866289523233817 1101 20-21 0.8651498642933646 1101 22-23 0.8330854247855015 1101 24-25 0.8665789476086161 1101 26-27 0.9664529509024504 1101 28-29 1.0142523748374046 1101 30-31 1.0324973699577669 1101 32-33 1.0505500734152236 1101 34-35 1.164189273734202 1101 36-37 1.276151270924565 1101 38-39 1.270166239188228 1101 40-41 1.1896811516591654 1101 42-43 1.4156262194153797 1101 44-45 1.512209951358379 1101 46-47 1.6227796968414552 1101 48-49 1.4230024133791446 1101 50-51 1.602482313842625 1101 52-53 1.6773207521584546 1101 54-55 1.8639670858490867 1101 56-57 2.0295662591889503 1101 58-59 2.014079060328239 1101 60-61 2.1121722353460832 1101 62-63 2.01575464127885 1101 64-65 1.9940877991216404 1101 66-67 2.3548406342835975 1101 68-69 2.436455112884879 1101 70-71 2.8202223836917746 1101 72-73 3.072978722384491 1101 74-75 2.9581018547430844 1101 76-77 3.2482908922412186 1101 78-79 3.29725951450688 1101 80-81 3.4880481421622456 1101 82-83 3.5849855900066174 1101 84-85 3.538572822292828 1101 86-87 3.608427154551304 1101 88-89 3.8061195840058026 1101 90-91 3.9839223311270597 1101 92-93 3.9091827254121885 1101 94-95 3.681520407428593 1101 96-97 3.5946137115889236 1101 98-99 3.4445198847838174 1101 100-101 2.6701492983652173 1102 1 0.5817711179235694 1102 2 0.4865386008110235 1102 3 0.50206616210939 1102 4 0.277244867062592 1102 5 0.33368709955419007 1102 6 0.3684987192581275 1102 7 0.40592024819783745 1102 8 0.5133898840091362 1102 9 0.5597674211182877 1102 10-11 0.6665817740845768 1102 12-13 0.7004049904973044 1102 14-15 0.7007939118641531 1102 16-17 0.8858303037810629 1102 18-19 0.8561497429474443 1102 20-21 0.7840734215472622 1102 22-23 0.7417429612992521 1102 24-25 0.8181276643364868 1102 26-27 0.8884702592221672 1102 28-29 0.8215092239807618 1102 30-31 0.6722814351559094 1102 32-33 0.7892809750939165 1102 34-35 0.793357491149024 1102 36-37 1.0151581140551613 1102 38-39 1.1552718008063678 1102 40-41 1.123980181560043 1102 42-43 1.3921771408213601 1102 44-45 1.3501477328285105 1102 46-47 1.392479905460398 1102 48-49 1.4336749917084077 1102 50-51 1.6883259568486402 1102 52-53 1.633058051548037 1102 54-55 1.6489589621707843 1102 56-57 1.876817124108964 1102 58-59 1.8297893043429632 1102 60-61 1.9455217046131459 1102 62-63 1.9966421543217798 1102 64-65 2.1947382369116006 1102 66-67 2.4810386169589727 1102 68-69 2.4856190269311043 1102 70-71 2.607193306807144 1102 72-73 2.7731921990891664 1102 74-75 2.623406580662529 1102 76-77 2.684519745845293 1102 78-79 3.3461830540961763 1102 80-81 3.76239848908822 1102 82-83 3.8410043272566803 1102 84-85 3.911110971376896 1102 86-87 3.972903960997961 1102 88-89 4.110009560173541 1102 90-91 4.118287779928179 1102 92-93 4.206808901959549 1102 94-95 4.176566302100554 1102 96-97 3.9807264612050233 1102 98-99 3.42309187435192 1102 100-101 2.285957098536727 1103 1 0.5099799902417779 1103 2 0.45978124043142543 1103 3 0.36277634533607284 1103 4 0.39020929556020434 1103 5 0.363158272780538 1103 6 0.39258253146763167 1103 7 0.40640329251844065 1103 8 0.38025871757440655 1103 9 0.4741884424163203 1103 10-11 0.5579180545813642 1103 12-13 0.5146352059937129 1103 14-15 0.5813088792856789 1103 16-17 0.5801063801510935 1103 18-19 0.7229107976605462 1103 20-21 0.6693168195406614 1103 22-23 0.7141766170070056 1103 24-25 0.7541855455670259 1103 26-27 0.7557727123821891 1103 28-29 0.840237142248867 1103 30-31 0.8381589866497166 1103 32-33 1.067963214371403 1103 34-35 0.9183291954934774 1103 36-37 1.014025299338769 1103 38-39 1.151121705426526 1103 40-41 1.2173681351464083 1103 42-43 1.4125318904155932 1103 44-45 1.4095357585090724 1103 46-47 1.5972040064262814 1103 48-49 1.6462928803882377 1103 50-51 1.633680299512644 1103 52-53 1.6611604234669848 1103 54-55 1.7397867479081448 1103 56-57 1.9444442845378695 1103 58-59 1.9638202314368165 1103 60-61 2.23274885294288 1103 62-63 2.361734136043065 1103 64-65 2.547085723575325 1103 66-67 2.6184500964512374 1103 68-69 2.7469302543638534 1103 70-71 2.822084007979914 1103 72-73 3.2629532081338226 1103 74-75 3.154732499100792 1103 76-77 3.2096313287538543 1103 78-79 3.37800809636585 1103 80-81 3.37999908866945 1103 82-83 3.635268436253096 1103 84-85 3.973517068516518 1103 86-87 3.8708960716255234 1103 88-89 3.912782333423241 1103 90-91 3.9647185008616166 1103 92-93 3.9544995645942134 1103 94-95 3.795025310320284 1103 96-97 3.604148601544752 1103 98-99 3.1914110336623747 1103 100-101 2.3018308706864996 1104 1 0.5947851683312919 1104 2 0.48759534296055307 1104 3 0.5451744468652748 1104 4 0.46831891196256237 1104 5 0.44574734481837197 1104 6 0.5538327664453035 1104 7 0.5251417885323733 1104 8 0.45523259103315894 1104 9 0.5820249044040011 1104 10-11 0.6096772189586019 1104 12-13 0.7192286227005127 1104 14-15 0.8688666193138275 1104 16-17 0.7484306830671983 1104 18-19 0.7627283843071737 1104 20-21 0.7028862010695036 1104 22-23 0.7779435434593651 1104 24-25 0.897206721085297 1104 26-27 0.8534615488719552 1104 28-29 1.0242714075735009 1104 30-31 0.9867987325216703 1104 32-33 1.1075924158838006 1104 34-35 1.1325097012497523 1104 36-37 1.1686521709142426 1104 38-39 1.213246175145784 1104 40-41 1.2425275479675761 1104 42-43 1.3032219200215422 1104 44-45 1.3011316921429454 1104 46-47 1.3623214717029057 1104 48-49 1.463826021540008 1104 50-51 1.5692306358221053 1104 52-53 1.6409346427831188 1104 54-55 1.8497482078498457 1104 56-57 2.0697150668560447 1104 58-59 2.1577259769943105 1104 60-61 2.2532193452031173 1104 62-63 2.3590433006930986 1104 64-65 2.3916784347291156 1104 66-67 2.606407242723659 1104 68-69 2.8119507464008997 1104 70-71 2.766000882427189 1104 72-73 3.0921874933974607 1104 74-75 2.9818124917005484 1104 76-77 3.0647686164741543 1104 78-79 3.1941942698579844 1104 80-81 3.3440643685669187 1104 82-83 3.462457237775208 1104 84-85 3.5971203463496515 1104 86-87 3.6708055153223924 1104 88-89 3.6923810882771733 1104 90-91 3.636986987714524 1104 92-93 3.697262004275009 1104 94-95 3.788709432872732 1104 96-97 3.5450841735188483 1104 98-99 3.229790978547401 1104 100-101 2.3663846313460546 1105 1 0.5507770486565349 1105 2 0.38592575798408646 1105 3 0.3154874023566201 1105 4 0.3265715486491345 1105 5 0.3068564908709135 1105 6 0.39778184732015376 1105 7 0.4543222910577214 1105 8 0.43407975104611296 1105 9 0.5497210428344417 1105 10-11 0.549541738325324 1105 12-13 0.5264124007279207 1105 14-15 0.6170270871043257 1105 16-17 0.6519100790720245 1105 18-19 0.6517744317224299 1105 20-21 0.6954750156501674 1105 22-23 0.7555262476310602 1105 24-25 0.8114599265574185 1105 26-27 0.5794082824381803 1105 28-29 0.8111812213555893 1105 30-31 0.8765859696957463 1105 32-33 0.939338839134308 1105 34-35 0.9832903214253932 1105 36-37 1.0120024958056746 1105 38-39 1.0657643686866933 1105 40-41 1.2582874891329325 1105 42-43 1.2409742651936568 1105 44-45 1.2775921368591696 1105 46-47 1.3888708307034392 1105 48-49 1.4054610520825506 1105 50-51 1.2583298703830046 1105 52-53 1.3320472381069273 1105 54-55 1.681095519792155 1105 56-57 1.7670185462283108 1105 58-59 1.9018302869454011 1105 60-61 2.1270279460191723 1105 62-63 2.044317001435939 1105 64-65 2.2094909178151028 1105 66-67 2.3467672654560907 1105 68-69 2.506763593167797 1105 70-71 2.502048485219266 1105 72-73 2.566601520845399 1105 74-75 2.648738704357971 1105 76-77 2.871790376082025 1105 78-79 2.9970569632237023 1105 80-81 3.111533158952831 1105 82-83 3.3921914516024856 1105 84-85 3.3362392367715508 1105 86-87 3.2534587669430515 1105 88-89 3.094578224218818 1105 90-91 2.843577980107609 1105 92-93 2.909140079064139 1105 94-95 3.038057086181329 1105 96-97 2.792083034523424 1105 98-99 2.4284899528343065 1105 100-101 1.6348936630717539 1106 1 0.2904858569077895 1106 2 0.22350289059821904 1106 3 0.07201557834333272 1106 4 0.17556946988119648 1106 5 0.20519476694818906 1106 6 -0.20313787845140752 1106 7 0.28402509293659506 1106 8 -0.15761479676320533 1106 9 0.1787727633316578 1106 10-11 0.18434127564694336 1106 12-13 0.2964905774185098 1106 14-15 0.4291182549214341 1106 16-17 -0.1654421782447173 1106 18-19 0.2640960002302677 1106 20-21 0.40431294683027375 1106 22-23 0.23884155402644325 1106 24-25 0.3817817015343081 1106 26-27 0.30078592371697255 1106 28-29 0.4331165636953429 1106 30-31 0.2769469385986909 1106 32-33 0.5239236955679303 1106 34-35 0.2504210351044449 1106 36-37 0.47112942500086774 1106 38-39 0.6195516093686066 1106 40-41 0.807658110463997 1106 42-43 0.40651745476508694 1106 44-45 0.8685797627935585 1106 46-47 1.1093733247557154 1106 48-49 0.9218552812725918 1106 50-51 0.655857755436049 1106 52-53 0.960497558837826 1106 54-55 0.9054613899888189 1106 56-57 1.332613001126557 1106 58-59 1.4391873423213433 1106 60-61 1.6885141360551614 1106 62-63 1.6891139227008445 1106 64-65 1.704804304986805 1106 66-67 1.9241340342623694 1106 68-69 2.025269027057817 1106 70-71 1.8477555126749614 1106 72-73 1.917341993941072 1106 74-75 2.4307958399752927 1106 76-77 2.5583508832450974 1106 78-79 2.8591648479952063 1106 80-81 2.719776787740244 1106 82-83 2.903485953894762 1106 84-85 2.9317141625130816 1106 86-87 2.8276381111222655 1106 88-89 2.7714374428273096 1106 90-91 2.7484386252295447 1106 92-93 2.824281240546396 1106 94-95 2.6300025642744913 1106 96-97 2.4149131738431073 1106 98-99 2.265541879800571 1106 100-101 1.6412019964441917 1107 1 0.286956105536202 1107 2 0.21792691397389063 1107 3 0.2895144225412061 1107 4 0.19265529986099494 1107 5 0.1239100501823387 1107 6 0.19387821623554657 1107 7 0.19782656598647463 1107 8 0.2526568110429608 1107 9 0.35334608445789684 1107 10-11 0.35482021044148837 1107 12-13 0.27582255795093147 1107 14-15 0.40915376594539765 1107 16-17 0.3455388814037761 1107 18-19 0.42425979959668325 1107 20-21 0.4976157282709366 1107 22-23 0.5018266777712839 1107 24-25 0.41270951457448746 1107 26-27 0.4952410911203984 1107 28-29 0.6484085090640548 1107 30-31 0.5979363621551954 1107 32-33 0.6953505019784743 1107 34-35 0.642211822393584 1107 36-37 0.7841391166579115 1107 38-39 0.8102918408478743 1107 40-41 0.7189259286056497 1107 42-43 0.863270214456179 1107 44-45 0.7404361518171996 1107 46-47 0.7523565809976205 1107 48-49 0.777740249923589 1107 50-51 1.0263940817844706 1107 52-53 0.9977767038403194 1107 54-55 1.2786999304669457 1107 56-57 1.4094437518399623 1107 58-59 1.3697273922884285 1107 60-61 1.6163301084434458 1107 62-63 1.7273660481241606 1107 64-65 1.738319150496487 1107 66-67 1.7797645556373105 1107 68-69 1.9546120247188803 1107 70-71 2.197638195912301 1107 72-73 2.056803469860533 1107 74-75 2.188884860652191 1107 76-77 2.301760647552559 1107 78-79 2.529610190511189 1107 80-81 2.698170518851704 1107 82-83 2.772933121780998 1107 84-85 2.8521371860914435 1107 86-87 2.6801162818348097 1107 88-89 2.8214789776869864 1107 90-91 2.7097033972916993 1107 92-93 2.7291520924693575 1107 94-95 2.5110948371394457 1107 96-97 2.356180804095745 1107 98-99 2.233938093324481 1107 100-101 1.3389649784364526 1108 1 0.3187590690916764 1108 2 0.2973957747124949 1108 3 0.1789261087293461 1108 4 0.1617137107467954 1108 5 0.23045976718631778 1108 6 0.3023714504187609 1108 7 0.19344473447756627 1108 8 0.32040900676695827 1108 9 0.38858123063921823 1108 10-11 0.3066143925804212 1108 12-13 0.35647860308168333 1108 14-15 0.3951210886210319 1108 16-17 0.4122303043664104 1108 18-19 0.370260827656935 1108 20-21 0.42173373591185737 1108 22-23 0.47480204098280154 1108 24-25 0.5134924612016505 1108 26-27 0.45878678777423687 1108 28-29 0.5049972262753144 1108 30-31 0.595023141191767 1108 32-33 0.7112117917617695 1108 34-35 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-0.7798839966830098 2315 94-95 -0.7476024421865652 2315 96-97 -0.7155002480009802 2315 98-99 -0.6960614342067704 2315 100-101 -0.30445937558686786 2316 1 -1.0526680892992992 2316 2 -0.9279278707712209 2316 3 -0.9199410342826724 2316 4 -0.8772641808829604 2316 5 -0.5989709319415795 2316 6 -0.5509801230744387 2316 7 -0.7394280124659218 2316 8 -0.7520868551085371 2316 9 -0.8214792457731903 2316 10-11 -0.9015044065196349 2316 12-13 -0.9428902002095896 2316 14-15 -1.1140502079391652 2316 16-17 -1.1448651122345979 2316 18-19 -1.0326238167437651 2316 20-21 -1.035815728786389 2316 22-23 -1.1858065622926048 2316 24-25 -1.0451652720337705 2316 26-27 -1.2159291317947094 2316 28-29 -1.1744739040892895 2316 30-31 -1.080025700412783 2316 32-33 -1.2990126169698186 2316 34-35 -1.1256450679289571 2316 36-37 -1.2909257847357338 2316 38-39 -1.3140381848433123 2316 40-41 -1.0599221375965797 2316 42-43 -0.8588475519706407 2316 44-45 -1.1099344286868984 2316 46-47 -0.7185288446129761 2316 48-49 -0.4982717684788902 2316 50-51 -0.5442781204407545 2316 52-53 -0.5451064500812066 2316 54-55 -0.6347108355883648 2316 56-57 -0.5695639162330401 2316 58-59 -0.08583936832110695 2316 60-61 -0.4574426856106548 2316 62-63 -0.21664338207920153 2316 64-65 -0.10241802046494897 2316 66-67 0.0949125914231459 2316 68-69 0.03481808173333789 2316 70-71 -0.06626815527691221 2316 72-73 0.0315253450815014 2316 74-75 0.14738082627156857 2316 76-77 0.3140228301025303 2316 78-79 0.387950791229283 2316 80-81 0.23631415941302691 2316 82-83 0.3111919742413036 2316 84-85 0.6003941922431899 2316 86-87 0.7273158414144554 2316 88-89 0.4216819032723329 2316 90-91 0.6712349972274989 2316 92-93 0.5893895928481587 2316 94-95 0.3127614252397919 2316 96-97 0.37147528189346346 2316 98-99 0.3217852021399583 2316 100-101 0.3581446730828315 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14879.0 3 5827.0 4 6649.0 5 7684.0 6 9183.0 7 11573.0 8 15001.0 9 18969.0 10 23629.0 11 29113.0 12 34960.0 13 40379.0 14 45345.0 15 51135.0 16 57842.0 17 64678.0 18 68365.0 19 72171.0 20 79781.0 21 88501.0 22 97762.0 23 108677.0 24 119816.0 25 131394.0 26 144143.0 27 157288.0 28 172854.0 29 188602.0 30 205877.0 31 225587.0 32 250386.0 33 275762.0 34 301833.0 35 318695.0 36 301686.0 37 196802.0 38 43309.0 39 511.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.41782961216795 17.93996156657869 13.900977153746025 24.74123166750734 2 15.842389306612409 19.229098748435263 39.58824646699742 25.340265477954908 3 19.71163340405324 22.574798536085346 28.422201618235448 29.291366441625964 4 13.378237881681759 15.479421425447132 35.26971964394563 35.87262104892548 5 14.829301987494329 36.25615946539655 33.48781359560173 15.426724951507387 6 34.64598843782665 34.15024802079033 16.69194349668532 14.511820044697702 7 30.622472651628357 30.830042997072887 19.723674486939654 18.823809864359102 8 26.920849386939206 34.910388383086335 19.066890902730833 19.10187132724363 9 26.602322182787585 16.672596918209702 19.174318280364226 37.55076261863849 10-11 25.830861139114326 25.61710547199201 26.76304512431441 21.788988264579253 12-13 26.38314667397321 22.69172369572109 27.096500699980442 23.828628930325255 14-15 23.237156467359075 24.41378010824837 24.8059838067783 27.543079617614254 16-17 23.508522754001326 27.539034789124262 25.52123221313615 23.431210243738256 18-19 23.50193322410596 26.49301103731712 27.064610582677005 22.94044515589991 20-21 23.816722887884055 25.82072585027194 27.26770654695 23.094844714894002 22-23 23.65011220496718 25.53059142625329 27.110205514434714 23.709090854344815 24-25 23.57968571811795 25.79176181016286 27.14352195909174 23.485030512627446 26-27 23.263293937244704 26.074463588426156 27.115174246172003 23.54706822815714 28-29 23.29689994847445 26.2846290392574 27.130729488609866 23.28774152365828 30-31 23.418631033019622 25.959470523777778 26.978064621030757 23.643833822171846 32-33 23.250693046054238 25.850413958349616 27.148173039395076 23.75071995620107 34-35 23.786404478312253 25.997809521180905 26.8132296891427 23.402556311364144 36-37 23.811502481270498 25.691095466341473 26.87908173858932 23.618320313798698 38-39 23.272294443755932 25.9594238690148 26.96090704604422 23.807374641185056 40-41 23.705685944272105 25.86737809906911 26.910819536661627 23.516116419997157 42-43 23.53861435841176 25.720703391307044 26.805501182424823 23.93518106785637 44-45 23.434721861080497 26.014107920562452 26.963458059874306 23.587712158482745 46-47 23.639455931650375 26.233651738100654 26.452977080602064 23.673915249646907 48-49 23.686603792708613 25.9756861828155 26.629844123027702 23.707865901448184 50-51 23.728332236840043 26.200528449140375 26.495772595026455 23.575366718993124 52-53 23.71213494293862 26.077650138506414 26.448309230855106 23.761905687699866 54-55 23.444039559332477 26.06965930448063 26.672484067996617 23.81381706819028 56-57 23.49132162335527 26.30988436106081 26.60468250279019 23.594111512793738 58-59 23.583338810170158 25.912645874085598 26.657662914155473 23.84635240158877 60-61 23.530849762445207 26.03244050594235 26.70831051062208 23.728399220990365 62-63 23.33101322736357 26.13796933069939 26.732525230682047 23.79849221125499 64-65 23.303210554411653 26.01215346763156 26.714111046891997 23.970524931064794 66-67 23.327328082124946 26.097921214816665 26.544452028352612 24.030298674705776 68-69 23.318978249131263 25.864751150319492 26.790149238942227 24.026121361607018 70-71 23.39128207482535 25.8985516954002 26.580908851213046 24.1292573785614 72-73 23.43222810960747 25.88399522202375 26.51503377960976 24.168742888759024 74-75 23.337698420164692 26.2338457293019 26.286452551631577 24.14200329890183 76-77 23.607701794704848 25.974862417333437 26.30698986547208 24.11044592248963 78-79 23.645226526104697 25.870795222970163 26.39401427569959 24.08996397522555 80-81 23.540194625246492 26.078016925254254 26.246549479093172 24.135238970406082 82-83 23.6824983326495 26.007180676822 26.12787465086615 24.182446339662345 84-85 23.647124518729537 25.86296297039578 26.11282976496642 24.37708274590826 86-87 23.751685826316084 25.747460866190824 26.115962776302748 24.384890531190344 88-89 23.72943012608922 25.816755047118818 25.998654787383902 24.455160039408057 90-91 23.776977623043656 25.79520917458723 25.963526860828107 24.464286341541012 92-93 23.809548298727552 25.44383351845081 26.074495620210435 24.672122562611204 94-95 23.699777635550614 25.507056427080244 25.998363188911433 24.794802748457712 96-97 23.76383572201658 25.43405743979118 25.832313482750852 24.969793355441386 98-99 23.816252422435042 25.374977265172756 25.619422127728964 25.189348184663242 100-101 24.85089857662529 24.312670535047296 25.665862878300715 25.1705680100267 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 257.0 1 195.5 2 186.0 3 260.0 4 249.0 5 241.5 6 408.0 7 842.0 8 1328.0 9 1544.5 10 1416.5 11 1242.5 12 1276.0 13 1466.5 14 1653.0 15 1803.5 16 2044.5 17 2286.5 18 2548.0 19 2803.5 20 3201.5 21 3809.5 22 4570.0 23 5593.5 24 7161.5 25 9241.5 26 11914.5 27 15321.5 28 18853.5 29 23015.0 30 28300.5 31 35110.5 32 43754.5 33 50457.5 34 55881.0 35 62594.5 36 70981.5 37 79759.5 38 86813.0 39 93219.5 40 99841.0 41 109252.0 42 118588.0 43 126132.0 44 133736.5 45 142040.5 46 149104.5 47 154782.0 48 159203.0 49 160274.5 50 160376.0 51 159355.0 52 159187.0 53 160918.0 54 161089.5 55 153527.0 56 140119.0 57 127615.5 58 116410.0 59 103666.0 60 88373.0 61 74884.0 62 62689.5 63 50685.5 64 40530.5 65 32215.5 66 25257.5 67 19816.5 68 15497.0 69 11753.0 70 9109.0 71 6975.0 72 5348.0 73 4232.0 74 3198.5 75 2495.5 76 1795.0 77 1151.5 78 725.0 79 458.0 80 300.5 81 171.5 82 109.5 83 70.5 84 32.5 85 21.5 86 16.5 87 8.5 88 6.5 89 5.5 90 6.5 91 5.5 92 3.0 93 1.5 94 1.0 95 0.5 96 1.0 97 1.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39193327326616245 2 0.029021875018812794 3 0.03619582165267664 4 0.043921610335299226 5 0.06361233798419123 6 0.052751083115439336 7 0.037450008127128356 8 0.03918078546187173 9 0.03591990062829725 10-11 0.03738729880340577 12-13 0.029586258932316072 14-15 0.02737889073728104 16-17 0.02198588889713865 18-19 0.027040260389179078 20-21 0.03385049294545192 22-23 0.03279697630691247 24-25 0.04160136535756355 26-27 0.03608294486997598 28-29 0.031266868808081376 30-31 0.03388811853968547 32-33 0.029159835531002484 34-35 0.0305519825176439 36-37 0.03237055290559889 38-39 0.015301074988310981 40-41 0.012830327633641093 42-43 0.014034346649114746 44-45 0.011224968946342893 46-47 0.019778520702103623 48-49 0.017157270970499527 50-51 0.011425638782255167 52-53 0.009293521775687244 54-55 0.00832779819035942 56-57 0.009055226345541418 58-59 0.004063564177223572 60-61 0.007637995629410975 62-63 0.012027648289991994 64-65 0.007424783928754182 66-67 0.003436470939997712 68-69 0.006408892884448288 70-71 0.013520130194589539 72-73 0.011074466569408687 74-75 0.01186460404831327 76-77 0.00568146472926629 78-79 0.009117935669264002 80-81 0.011902229642546822 82-83 0.013382169682399851 84-85 0.008127128354447144 86-87 0.002545998543136991 88-89 0.016580345192251735 90-91 0.025836241373705427 92-93 0.011049382839919651 94-95 0.021296086336190203 96-97 0.019314471706556487 98-99 0.01186460404831327 100-101 0.1223709743122543 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3986648.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.52841653249082 #Duplication Level Percentage of deduplicated Percentage of total 1 74.04243858914667 36.67204739524628 2 13.691840027547187 13.562703119611754 3 4.929685243285151 7.32478512310501 4 2.3580662399782804 4.671651477793949 5 1.3120119453414216 3.2490937062236753 6 0.7971984818688462 2.3690387081441733 7 0.5220518519628973 1.809948110290363 8 0.3835664842841524 1.5197952481222876 9 0.2842135777495239 1.2668983616671097 >10 1.4746816193936219 13.342184641942778 >50 0.12001471947597891 4.113220012305628 >100 0.07819589159070152 7.173142103411968 >500 0.004500922899238831 1.512815159509819 >1k 0.0014832586271365249 1.2639006780146966 >5k 5.1146849211604284E-5 0.14877615461047264 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5873 0.147316743289099 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4516 0.11327812237247933 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 2.5083729489034395E-5 7 0.0 0.0 0.0 0.0 2.5083729489034395E-5 8 0.0 0.0 0.0 0.0 2.5083729489034395E-5 9 0.0 0.0 0.0 0.0 2.5083729489034395E-5 10-11 0.0 0.0 0.0 0.0 3.762559423355159E-5 12-13 0.0 0.0 0.0 0.0 7.525118846710319E-5 14-15 0.0 0.0 0.0 0.0 1.0033491795613758E-4 16-17 0.0 0.0 0.0 0.0 1.25418647445172E-4 18-19 0.0 0.0 0.0 2.5083729489034395E-5 1.25418647445172E-4 20-21 0.0 0.0 0.0 6.2709323722586E-5 1.3796051218968917E-4 22-23 0.0 0.0 0.0 2.2575356540130957E-4 1.6304424167872358E-4 24-25 0.0 0.0 0.0 3.8879780708003315E-4 1.7558610642324078E-4 26-27 0.0 0.0 0.0 6.396351019703772E-4 1.7558610642324078E-4 28-29 0.0 0.0 0.0 0.0024205798956918193 1.7558610642324078E-4 30-31 0.0 0.0 0.0 0.009243354316709175 1.7558610642324078E-4 32-33 0.0 0.0 0.0 0.02448171998129757 1.7558610642324078E-4 34-35 0.0 0.0 0.0 0.047972632647778284 1.7558610642324078E-4 36-37 0.0 0.0 0.0 0.09055226345541417 1.7558610642324078E-4 38-39 0.0 2.5083729489034395E-5 0.0 0.15327412904274468 1.7558610642324078E-4 40-41 0.0 2.5083729489034395E-5 0.0 0.22383466009539843 1.7558610642324078E-4 42-43 0.0 2.5083729489034395E-5 0.0 0.29643951510140854 1.7558610642324078E-4 44-45 0.0 2.5083729489034395E-5 0.0 0.38672839939718784 1.8812797116775798E-4 46-47 0.0 2.5083729489034395E-5 0.0 0.49525315503149514 2.3829543014582678E-4 48-49 0.0 5.016745897806879E-5 0.0 0.6106759362752869 3.0100475386841274E-4 50-51 0.0 5.016745897806879E-5 0.0 0.7356179928601673 3.0100475386841274E-4 52-53 0.0 5.016745897806879E-5 0.0 0.8592556955116177 3.386303481019644E-4 54-55 0.0 5.016745897806879E-5 0.0 0.9991601967367071 3.7625594233551597E-4 56-57 0.0 5.016745897806879E-5 0.0 1.1619034336615623 3.7625594233551597E-4 58-59 0.0 5.016745897806879E-5 0.0 1.3351692951070673 3.7625594233551597E-4 60-61 0.0 5.016745897806879E-5 0.0 1.5238365664588396 3.7625594233551597E-4 62-63 0.0 5.016745897806879E-5 0.0 1.717332455737251 4.013396718245503E-4 64-65 0.0 6.2709323722586E-5 0.0 1.9158952583724473 4.013396718245503E-4 66-67 0.0 7.525118846710319E-5 0.0 2.1477567119043366 4.2642340131358473E-4 68-69 0.0 7.525118846710319E-5 0.0 2.404614101872049 4.2642340131358473E-4 70-71 0.0 7.525118846710319E-5 0.0 2.6777257485486556 4.2642340131358473E-4 72-73 0.0 7.525118846710319E-5 0.0 2.9531576402029978 5.267583192697223E-4 74-75 0.0 7.525118846710319E-5 0.0 3.23780780244456 5.64383913503274E-4 76-77 0.0 7.525118846710319E-5 0.0 3.5375207442442873 5.894676429923083E-4 78-79 0.0 7.525118846710319E-5 0.0 3.8727146214062538 6.020095077368255E-4 80-81 0.0 7.525118846710319E-5 0.0 4.229116791851199 6.396351019703772E-4 82-83 0.0 7.525118846710319E-5 0.0 4.590874338542053 6.521769667148943E-4 84-85 0.0 7.525118846710319E-5 0.0 4.95280747133933 6.521769667148943E-4 86-87 0.0 7.525118846710319E-5 0.0 5.32365786996996 6.521769667148943E-4 88-89 2.5083729489034395E-5 7.525118846710319E-5 0.0 5.71655686682145 7.274281551819975E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5510 0.0 39.747314 1 GTATCAA 8655 0.0 37.76332 1 ATCAACG 10775 0.0 29.99246 3 TATCAAC 10830 0.0 29.840145 2 TCAACGC 10900 0.0 29.735712 4 CAACGCA 11190 0.0 28.709898 5 AACGCAG 11930 0.0 26.881805 6 GTACATG 11455 0.0 26.241713 1 TACATGG 11260 0.0 25.5773 2 ACATGGG 11975 0.0 23.216717 3 ACGCAGA 14015 0.0 22.878601 7 CGCAGAG 13915 0.0 22.838192 8 CATGGGG 8830 0.0 20.613821 4 GCAGAGT 14960 0.0 20.511782 9 TAAGGTG 1565 0.0 20.042166 5 GTACCGT 270 2.296838E-8 19.356373 6 GAGTACT 7395 0.0 19.270412 12-13 AGAGTAC 12975 0.0 18.725697 10-11 GTAAGGT 1420 0.0 18.407501 4 GTACTTT 7870 0.0 18.017696 14-15 >>END_MODULE