FastQCFastQC Report
Thu 26 May 2016
SRR938388_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938388_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3986648
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63500.15928168225536843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51620.12948221162239557No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53950.041.4820331
GTATCAA83250.039.4959371
ATCAACG107050.029.9938743
TATCAAC110000.029.4515272
TCAACGC109800.029.285924
CAACGCA111250.028.7761365
AACGCAG115500.027.7583966
TACATGG110750.024.0193062
GTACATG115250.023.7471831
CGCAGAG135300.023.4504538
GGACCGA14200.023.4143586
ACGCAGA137150.023.3765567
ACATGGG115250.022.2548873
GCAGAGT153300.020.0153379
AGGACCG16150.019.9990315
GCACCGT11750.019.4033156
AGAGTAC133600.019.30482710-11
ACCGAGT15650.019.1204788
GAGTACT77650.019.11530112-13
TAAGGTG16400.019.1149255
Produced by FastQC (version 0.11.5)