##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938388_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3986648 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81316133252798 31.0 31.0 34.0 30.0 34.0 2 32.07371832175803 33.0 31.0 34.0 30.0 34.0 3 32.13825073093988 33.0 31.0 34.0 30.0 34.0 4 35.78123400912245 37.0 35.0 37.0 35.0 37.0 5 35.667597691092865 37.0 35.0 37.0 33.0 37.0 6 35.69670936586325 37.0 35.0 37.0 33.0 37.0 7 35.61646350518029 37.0 35.0 37.0 33.0 37.0 8 35.58757231639212 37.0 35.0 37.0 33.0 37.0 9 37.143455353971554 39.0 37.0 39.0 33.0 39.0 10-11 37.064468696508946 39.0 37.0 39.0 33.0 39.0 12-13 36.94064098460662 39.0 37.0 39.0 33.0 39.0 14-15 37.975459960347635 40.0 37.0 41.0 33.0 41.0 16-17 38.060236193413616 40.0 37.0 41.0 33.0 41.0 18-19 38.173964067055834 40.0 37.5 41.0 33.0 41.0 20-21 38.116277258488836 40.0 37.0 41.0 33.0 41.0 22-23 37.985767742725216 40.0 37.0 41.0 33.0 41.0 24-25 37.86248484942739 40.0 37.0 41.0 32.0 41.0 26-27 37.64536547997214 40.0 37.0 41.0 32.0 41.0 28-29 37.50307865153884 39.5 36.5 41.0 32.0 41.0 30-31 37.270118656073976 39.0 36.0 41.0 31.0 41.0 32-33 37.20765151575961 39.0 36.0 41.0 31.0 41.0 34-35 37.08463325580789 39.0 36.0 41.0 31.0 41.0 36-37 36.97289389482091 39.0 36.0 41.0 30.5 41.0 38-39 36.76505161729854 39.0 35.0 40.5 30.0 41.0 40-41 36.55285129261475 39.0 35.0 40.0 30.0 41.0 42-43 36.30278018024165 38.5 35.0 40.0 30.0 41.0 44-45 36.0340909204926 38.0 35.0 40.0 29.0 41.0 46-47 35.66239545101549 38.0 34.5 40.0 28.0 41.0 48-49 35.2747048397551 38.0 34.0 40.0 27.0 41.0 50-51 34.88219965745659 38.0 33.5 40.0 26.0 41.0 52-53 34.26451081209076 37.5 33.0 40.0 24.0 41.0 54-55 33.813981445063625 37.0 32.5 40.0 23.5 41.0 56-57 33.392370482671154 36.5 32.0 39.0 23.0 40.5 58-59 32.82354110520919 36.0 31.0 39.0 21.0 40.0 60-61 32.29469607048327 35.5 31.0 39.0 19.5 40.0 62-63 31.738657388362352 35.0 30.0 38.5 17.5 40.0 64-65 31.040701110306202 34.5 29.0 38.0 12.5 40.0 66-67 30.344104872062946 34.0 28.0 37.5 8.0 39.5 68-69 29.57325916408973 34.0 27.5 37.0 7.0 39.0 70-71 28.755430125759787 33.0 26.0 36.0 2.0 39.0 72-73 28.141931266567802 32.5 26.0 35.5 2.0 38.5 74-75 27.395748633940094 32.0 25.0 35.0 2.0 37.5 76-77 26.23944802751585 30.5 24.0 34.0 2.0 36.0 78-79 26.715778644114053 32.0 25.0 34.5 2.0 36.0 80-81 26.32242023875697 32.0 24.0 35.0 2.0 36.0 82-83 25.61210821722911 31.0 21.0 34.0 2.0 35.5 84-85 24.800695095227873 31.0 19.0 34.0 2.0 35.0 86-87 23.854157302074324 30.0 12.0 34.0 2.0 35.0 88-89 22.841079899705218 29.5 4.5 34.0 2.0 35.0 90-91 21.928607441640196 29.0 2.0 34.0 2.0 35.0 92-93 20.79200358295992 28.0 2.0 33.0 2.0 35.0 94-95 19.571752509877975 26.0 2.0 33.0 2.0 34.0 96-97 18.149077871936527 24.5 2.0 32.0 2.0 34.0 98-99 16.354104751661048 10.5 2.0 32.0 2.0 34.0 100-101 12.207045869111093 2.0 2.0 27.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2582459060679412 1101 2 0.27804444673545703 1101 3 0.23725829303705837 1101 4 0.1584611918561265 1101 5 0.08656085801789004 1101 6 0.13765414925838826 1101 7 0.19089518922557147 1101 8 0.24900346338827717 1101 9 0.2933363287272144 1101 10-11 0.27682254688834007 1101 12-13 0.3233623854032217 1101 14-15 0.4362879395871033 1101 16-17 0.3697899270587115 1101 18-19 0.38411002478059686 1101 20-21 0.38817411280925285 1101 22-23 0.3099749134228986 1101 24-25 0.3246705380212589 1101 26-27 0.45205932994870324 1101 28-29 0.3923290493919964 1101 30-31 0.5058757413812955 1101 32-33 0.39192418587956723 1101 34-35 0.3087076317058788 1101 36-37 0.4250740504976349 1101 38-39 0.5602979549081866 1101 40-41 0.5760034529542182 1101 42-43 0.641904932190215 1101 44-45 0.5375383421229358 1101 46-47 0.6943155729392387 1101 48-49 0.6787343611068763 1101 50-51 0.8256366674693396 1101 52-53 0.8393667440359138 1101 54-55 0.8543401340540626 1101 56-57 0.9465927188505816 1101 58-59 0.9007656677127756 1101 60-61 1.1270282885369127 1101 62-63 1.288476462914037 1101 64-65 1.4039413082144137 1101 66-67 1.520816568872636 1101 68-69 1.6151184554345441 1101 70-71 1.8075948833962094 1101 72-73 1.999963787668495 1101 74-75 1.9360836160457424 1101 76-77 1.838115588102486 1101 78-79 1.8620387142554726 1101 80-81 2.050230199847494 1101 82-83 2.3312120237692895 1101 84-85 2.4190907144838647 1101 86-87 2.4046700721142784 1101 88-89 2.220791162047231 1101 90-91 2.4636958647178346 1101 92-93 2.8131285440321463 1101 94-95 2.564210738078941 1101 96-97 2.6603676707597437 1101 98-99 2.6151499708302204 1101 100-101 1.9452910442385818 1102 1 0.256357967851649 1102 2 0.21667130391349332 1102 3 0.21589275414647346 1102 4 0.1601112694241067 1102 5 0.176694009704768 1102 6 0.23167188261557925 1102 7 0.22557650827123155 1102 8 0.25035983299206777 1102 9 0.24838311206449504 1102 10-11 0.30039099742155173 1102 12-13 0.3065056340402279 1102 14-15 0.486038726035531 1102 16-17 0.3042150890057371 1102 18-19 0.291490410632548 1102 20-21 0.2883733287455428 1102 22-23 0.30939384995683383 1102 24-25 0.23855876675229837 1102 26-27 0.3510549411106183 1102 28-29 0.24103673434954231 1102 30-31 0.23205292059179783 1102 32-33 0.30696744173189217 1102 34-35 0.43495181410729344 1102 36-37 0.30912829658536367 1102 38-39 0.37833836363444817 1102 40-41 0.4007715948732553 1102 42-43 0.3082258482601219 1102 44-45 0.27885979152791407 1102 46-47 0.33243739917092086 1102 48-49 0.5007677170373128 1102 50-51 0.674615605965414 1102 52-53 0.637431512247133 1102 54-55 0.6571619793499295 1102 56-57 0.6872359494832807 1102 58-59 0.9377273169125573 1102 60-61 0.8578420178997348 1102 62-63 0.9454663931402187 1102 64-65 1.162225428648405 1102 66-67 1.208651236630704 1102 68-69 1.342791384745837 1102 70-71 1.4855575189601318 1102 72-73 1.6071109056684385 1102 74-75 1.4337438377147471 1102 76-77 1.448508100974884 1102 78-79 1.623415634584454 1102 80-81 1.913844735966233 1102 82-83 2.120380559146934 1102 84-85 2.046035807875935 1102 86-87 1.9336427016301165 1102 88-89 1.7811844970029895 1102 90-91 2.115986569428074 1102 92-93 2.4057173130689833 1102 94-95 2.1421571493861364 1102 96-97 2.1567501302999936 1102 98-99 2.2763486960982497 1102 100-101 1.8614823972532708 1103 1 0.2263597952485128 1103 2 0.1914579719693208 1103 3 0.21582922689885464 1103 4 0.18884258745436 1103 5 0.143922121487158 1103 6 0.18074128422252045 1103 7 0.20329261277777277 1103 8 0.18879474698744048 1103 9 0.28355176998746856 1103 10-11 0.30383369317525677 1103 12-13 0.33873516826623273 1103 14-15 0.4488573944266534 1103 16-17 0.3571059708753026 1103 18-19 0.3577797425822524 1103 20-21 0.27008133655940014 1103 22-23 0.3616239561916643 1103 24-25 0.35502121374675255 1103 26-27 0.40677209355314403 1103 28-29 0.3698370756569247 1103 30-31 0.35811259495138614 1103 32-33 0.364999055236332 1103 34-35 0.4154223256354115 1103 36-37 0.5411694764761492 1103 38-39 0.3688260149984899 1103 40-41 0.4599119367776865 1103 42-43 0.4799307726300839 1103 44-45 0.586153378551117 1103 46-47 0.6238966879253951 1103 48-49 0.6759695725601418 1103 50-51 0.7257959639730842 1103 52-53 0.6778958318455963 1103 54-55 0.8833927555204397 1103 56-57 1.0132861211155841 1103 58-59 1.0756661911794083 1103 60-61 1.055973222885747 1103 62-63 1.1142210567749764 1103 64-65 1.2365503972752379 1103 66-67 1.310544625388264 1103 68-69 1.334115401320652 1103 70-71 1.4972001721294994 1103 72-73 1.5409614127430586 1103 74-75 1.1936328851361857 1103 76-77 1.4166372584592573 1103 78-79 1.558170021045349 1103 80-81 1.678368933026963 1103 82-83 1.776158102014481 1103 84-85 1.8182926680443536 1103 86-87 1.6401982193373144 1103 88-89 1.5758631805998 1103 90-91 2.109477300084343 1103 92-93 2.32808800497099 1103 94-95 1.9010882138214136 1103 96-97 1.874562821264778 1103 98-99 2.073305035811128 1103 100-101 1.937073199008653 1104 1 0.16761630704402108 1104 2 0.24510814861797314 1104 3 0.16880449063184244 1104 4 0.16857610417361002 1104 5 0.14610220589560186 1104 6 0.042618308192473364 1104 7 0.11198041313380003 1104 8 0.13670354489870817 1104 9 0.140823107572146 1104 10-11 0.2083645897012545 1104 12-13 0.23358048971816459 1104 14-15 0.30954855614139376 1104 16-17 0.2600149014652615 1104 18-19 0.3191301313680057 1104 20-21 0.24330370876502627 1104 22-23 0.31956351075831435 1104 24-25 0.319751468058314 1104 26-27 0.4408853956949983 1104 28-29 0.3080996681042123 1104 30-31 0.2803332802777234 1104 32-33 0.487196465993172 1104 34-35 0.38935738078263427 1104 36-37 0.36118501104229495 1104 38-39 0.45154091479012237 1104 40-41 0.44171713197903273 1104 42-43 0.48589830652763055 1104 44-45 0.4618983011734059 1104 46-47 0.5676682978113732 1104 48-49 0.6229933602365421 1104 50-51 0.6403399163297863 1104 52-53 0.7096938289182333 1104 54-55 0.7944988109260649 1104 56-57 0.9254288992535109 1104 58-59 0.9748298318627135 1104 60-61 1.0483668902188725 1104 62-63 1.244271309757277 1104 64-65 1.2297497776285766 1104 66-67 1.1576878672815987 1104 68-69 1.395236596976332 1104 70-71 1.5436589780014849 1104 72-73 1.8003067041872924 1104 74-75 1.7915633642327258 1104 76-77 1.7889627289477978 1104 78-79 1.8578628388876801 1104 80-81 2.0597257300692497 1104 82-83 2.0912791880552426 1104 84-85 2.345000918525841 1104 86-87 2.4475984288456374 1104 88-89 2.3211800879811477 1104 90-91 2.432475811107089 1104 92-93 2.5313652367132846 1104 94-95 2.3032060632462326 1104 96-97 2.5968300619300244 1104 98-99 2.629716572111274 1104 100-101 2.423943775042469 1105 1 0.08795891249961585 1105 2 0.18418423769035996 1105 3 0.12811484053464994 1105 4 0.14934066019328895 1105 5 0.08720168544258655 1105 6 0.04967020441332437 1105 7 0.11319442367959454 1105 8 0.15754314927465174 1105 9 0.19842593763630134 1105 10-11 0.2135056774774995 1105 12-13 0.23017181208283688 1105 14-15 0.283157357978979 1105 16-17 0.20021508976271463 1105 18-19 0.2766562608441063 1105 20-21 0.19825350798087982 1105 22-23 0.3046344332828852 1105 24-25 0.2752105867160921 1105 26-27 0.2872905096372733 1105 28-29 0.31164347978397444 1105 30-31 0.3884717919017433 1105 32-33 0.49602385857199494 1105 34-35 0.44671077369759615 1105 36-37 0.3918388960184771 1105 38-39 0.4215025217659587 1105 40-41 0.5191077523351026 1105 42-43 0.46879919113632695 1105 44-45 0.6085820630583214 1105 46-47 0.5647660315950489 1105 48-49 0.6436362279601937 1105 50-51 0.7239792844555737 1105 52-53 0.6830485051615156 1105 54-55 0.8264675376915562 1105 56-57 0.922280400647935 1105 58-59 1.11197610945095 1105 60-61 0.9971844999712189 1105 62-63 1.1588065934521161 1105 64-65 1.171527894768012 1105 66-67 1.2496160502729161 1105 68-69 1.5828205142090113 1105 70-71 1.56704482350262 1105 72-73 1.7213540007467572 1105 74-75 1.6019356410176329 1105 76-77 1.6922401063811598 1105 78-79 1.8567187395866505 1105 80-81 1.9144452969566252 1105 82-83 2.302615005591882 1105 84-85 2.392349881750391 1105 86-87 2.358352867319475 1105 88-89 2.2301151130162573 1105 90-91 2.3070544445214836 1105 92-93 2.3214452109042867 1105 94-95 2.050680712408102 1105 96-97 2.2016744957605177 1105 98-99 2.062830323997332 1105 100-101 2.1403126680756532 1106 1 -0.00859680833545795 1106 2 -0.01177237096163708 1106 3 0.009140100146019847 1106 4 0.08034198816468319 1106 5 0.009433123171049829 1106 6 0.024777310233403682 1106 7 0.047686251728414675 1106 8 0.06467958777737692 1106 9 -0.1401370846951977 1106 10-11 -0.006867304170221189 1106 12-13 0.0650270725857709 1106 14-15 -0.002094299639026076 1106 16-17 -0.02544654969540261 1106 18-19 0.04271673750606908 1106 20-21 0.07078954621519529 1106 22-23 0.1760570636985932 1106 24-25 0.08942310671778131 1106 26-27 -0.24917649989315294 1106 28-29 0.16315956090753758 1106 30-31 0.26960550642073855 1106 32-33 0.04870842547969545 1106 34-35 -0.032958887552389626 1106 36-37 -0.052224825492316995 1106 38-39 0.06217880175676527 1106 40-41 0.2592577122144135 1106 42-43 0.25060375410235025 1106 44-45 0.20739298902508096 1106 46-47 -0.18107566999147906 1106 48-49 -0.04420960769981974 1106 50-51 0.24869820499783657 1106 52-53 0.18902818907616847 1106 54-55 0.1343883239632433 1106 56-57 0.3709777099808065 1106 58-59 0.604631031626198 1106 60-61 0.7064352519408814 1106 62-63 0.5071194128339265 1106 64-65 0.6832167412160395 1106 66-67 0.6446404394295335 1106 68-69 0.6780207539946588 1106 70-71 0.443044790655275 1106 72-73 0.7712544028921577 1106 74-75 0.8094487095700487 1106 76-77 0.9259904055266617 1106 78-79 1.1053722329022158 1106 80-81 1.2739829353260816 1106 82-83 1.5830832680140468 1106 84-85 1.6175944771883657 1106 86-87 1.6623405941421474 1106 88-89 1.5672388935584287 1106 90-91 1.5498423398041332 1106 92-93 1.7019037950896951 1106 94-95 1.6871513586093059 1106 96-97 1.6598214594923526 1106 98-99 1.935040880196798 1106 100-101 1.8916550688414642 1107 1 0.08762263692507233 1107 2 0.08815970431239606 1107 3 0.07779804876236085 1107 4 0.024276361378312572 1107 5 0.003129560567572298 1107 6 0.003847498737329147 1107 7 0.024286098228785136 1107 8 0.10287030765421434 1107 9 0.047240758976585084 1107 10-11 0.09782143774770446 1107 12-13 0.046251266314150996 1107 14-15 0.19071598483633068 1107 16-17 0.1308617955375624 1107 18-19 0.09797216408244935 1107 20-21 0.17746663787543326 1107 22-23 0.23706784397320746 1107 24-25 0.12291241640215134 1107 26-27 0.2074498902008841 1107 28-29 0.20974234366911304 1107 30-31 0.22643979855741492 1107 32-33 0.22820187669346126 1107 34-35 0.24630906837453637 1107 36-37 0.30062530853518155 1107 38-39 0.351849119163937 1107 40-41 0.38191003896366027 1107 42-43 0.2914612454082146 1107 44-45 0.23464577832292832 1107 46-47 0.3937072612593582 1107 48-49 0.5681886475513878 1107 50-51 0.6421544860706945 1107 52-53 0.43028522922065093 1107 54-55 0.6164471434736427 1107 56-57 0.5681529694384579 1107 58-59 0.7146582983078957 1107 60-61 0.8023603377435293 1107 62-63 0.875677837208034 1107 64-65 0.9720367184871641 1107 66-67 1.0210334087364394 1107 68-69 1.302043759581224 1107 70-71 0.9838746110333751 1107 72-73 1.3310614693411011 1107 74-75 1.3539330567069605 1107 76-77 1.3474202864847378 1107 78-79 1.470807030858893 1107 80-81 1.590639894393604 1107 82-83 1.8608270739394932 1107 84-85 2.008250699503204 1107 86-87 2.2164642740794704 1107 88-89 2.1347239307604546 1107 90-91 2.387667131588941 1107 92-93 2.2963693533250407 1107 94-95 2.1886654024591863 1107 96-97 1.9719824906272656 1107 98-99 2.111225605844677 1107 100-101 1.7911605791138694 1108 1 0.040861511674158635 1108 2 0.036318935624478854 1108 3 0.07594839509570761 1108 4 -0.02675711534756431 1108 5 0.06314352338150542 1108 6 0.12213377127258696 1108 7 0.1339553386625525 1108 8 0.0850198018186461 1108 9 0.12843697136192844 1108 10-11 0.12936056428443266 1108 12-13 0.07433042925545408 1108 14-15 0.22211069964659202 1108 16-17 0.18551592164443065 1108 18-19 0.05208268730002885 1108 20-21 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-0.5611505423400445 2316 50-51 -0.7198320128680962 2316 52-53 -0.5823385828846241 2316 54-55 -0.3746038718838278 2316 56-57 -0.2541494559038995 2316 58-59 -0.3314184643555933 2316 60-61 -0.2613714559143645 2316 62-63 -0.4203275142808529 2316 64-65 -0.3259287504920465 2316 66-67 -0.17977018443816561 2316 68-69 0.06399476337822563 2316 70-71 -0.06248779632851509 2316 72-73 0.2370136819370785 2316 74-75 0.3014430654951674 2316 76-77 0.32024181073871816 2316 78-79 0.16340400695340662 2316 80-81 -0.24409262095208462 2316 82-83 0.20877890605000005 2316 84-85 0.3118830450333938 2316 86-87 0.36752303944339815 2316 88-89 0.3165066735556401 2316 90-91 0.5196140218778069 2316 92-93 0.6421050539738857 2316 94-95 0.791166084302624 2316 96-97 0.726717682839773 2316 98-99 0.6544130639660217 2316 100-101 0.40544705100340117 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 21.0 4 56.0 5 142.0 6 265.0 7 699.0 8 1693.0 9 3058.0 10 4875.0 11 7769.0 12 11119.0 13 16014.0 14 21762.0 15 28318.0 16 35510.0 17 43611.0 18 46837.0 19 47489.0 20 51010.0 21 56867.0 22 62947.0 23 70836.0 24 78903.0 25 89491.0 26 103116.0 27 119534.0 28 139781.0 29 163233.0 30 188833.0 31 219583.0 32 255043.0 33 302187.0 34 361676.0 35 432891.0 36 487707.0 37 411579.0 38 120107.0 39 2076.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.30220444542127 18.713587184645984 14.580731857915724 24.40347651201703 2 16.436346486243757 19.96249551576819 38.651218331288774 24.94993966669928 3 19.84926967659077 22.842655000890485 28.925873992560057 28.382201329958683 4 13.930349506653208 16.22204920023037 34.932579951879376 34.91502134123705 5 14.714629218905756 36.34307905558518 33.754105589722464 15.188186135786594 6 34.57576389989786 34.70690163766653 16.397183799522807 14.320150662912804 7 30.545811920189593 30.910805267984532 19.674598810830553 18.868784000995323 8 26.830687666117164 35.45929859801878 18.836495056995307 18.87351867886874 9 26.37760257018699 16.65547092837169 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34-35 23.721459259929155 26.029516186062928 27.004805389321074 23.24421916468684 36-37 23.831373184481418 25.676378028205477 27.047959640816917 23.44428914649619 38-39 23.274304191888966 25.972316315652357 27.101554549597314 23.651824942861367 40-41 23.653238183973826 25.843983950444464 27.04490343323414 23.457874432347573 42-43 23.535526596345118 25.751984848674887 26.918737222340678 23.793751332639314 44-45 23.409777780453396 26.016070503826487 27.075689797912794 23.498461917807326 46-47 23.60562212297137 26.22377696927252 26.609602789915876 23.560998117840235 48-49 23.589704935073275 25.994482081187005 26.790664738898446 23.62514824484128 50-51 23.663250429935122 26.22536526926882 26.616696533027245 23.494687767768813 52-53 23.727916284557853 26.10219161561292 26.50912495911352 23.66076714071571 54-55 23.471723613421602 26.050218128111634 26.709832420620028 23.76822583784673 56-57 23.49515476349489 26.319758644324587 26.667431720512035 23.51765487166849 58-59 23.560382055300593 25.955928388962356 26.720430296329145 23.763259259407903 60-61 23.496443628833045 26.137597299786684 26.717771421003313 23.648187650376958 62-63 23.274251953019178 26.234608623585526 26.750380269339054 23.74075915405624 64-65 23.199143640830826 26.103515612752076 26.6956425766528 24.001698169764303 66-67 23.246852002378454 26.15210648916633 26.578131382675778 24.022910125779433 68-69 23.268372003479154 25.96191870145067 26.730756326972653 24.038952968097522 70-71 23.26824731807866 26.007679384746208 26.626745906178062 24.097327390997073 72-73 23.293312162623163 25.991912989384545 26.53185389199928 24.18292095599302 74-75 23.196454766008937 26.349843276858152 26.214667058641744 24.239034898491163 76-77 23.341426180590812 26.13419845444092 26.484266983189887 24.040108381778378 78-79 23.31306902440346 26.041576783302666 26.63349008992015 24.01186410237372 80-81 23.178538421008867 26.309194420715887 26.46866453095031 24.043602627324937 82-83 23.30198166208173 26.232007551291165 26.395003465356343 24.071007321270766 84-85 23.22919100711804 26.10989636188565 26.37298959630315 24.28792303469316 86-87 23.253190557757648 25.9746827377822 26.450238988463408 24.32188771599674 88-89 23.135564012674305 26.177618390186442 26.199679530272046 24.487138066867203 90-91 23.196800778609315 26.109210993341435 26.357806599366125 24.336181628683125 92-93 23.108838403759766 25.82099093457495 26.425724681532436 24.644445980132843 94-95 22.922204684512486 25.870759830158047 26.398145308808967 24.8088901765205 96-97 22.97432868092802 25.792975175256906 26.302651538667515 24.930044605147557 98-99 22.905671631907307 25.790476109252687 26.182823264055415 25.121028994784588 100-101 23.142543094485166 25.396830970988905 26.292257629030107 25.168368305495818 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 316.0 1 229.5 2 197.5 3 271.5 4 238.0 5 238.0 6 461.5 7 991.5 8 1520.5 9 1732.0 10 1560.5 11 1302.5 12 1384.5 13 1603.5 14 1856.0 15 2035.5 16 2158.5 17 2418.0 18 2641.0 19 2921.5 20 3328.0 21 3890.5 22 4646.0 23 5654.0 24 7210.5 25 9299.5 26 12111.0 27 15630.5 28 19126.0 29 23298.5 30 28635.0 31 35506.0 32 44957.5 33 52027.5 34 56733.5 35 63124.0 36 72091.5 37 80940.5 38 87883.0 39 94358.5 40 101297.5 41 111784.0 42 121186.0 43 128666.5 44 136979.0 45 145646.5 46 152118.0 47 158747.0 48 162475.5 49 162121.0 50 163233.0 51 161114.0 52 159751.0 53 161603.0 54 163376.0 55 155504.0 56 139004.5 57 124932.0 58 113501.0 59 100779.0 60 84335.0 61 70184.0 62 58787.0 63 46750.0 64 36540.0 65 28245.5 66 21309.0 67 16940.0 68 13378.5 69 10114.5 70 7684.5 71 5658.0 72 4401.0 73 3537.5 74 2709.0 75 2142.5 76 1531.5 77 891.5 78 556.0 79 346.5 80 204.5 81 110.0 82 51.0 83 22.5 84 9.0 85 7.5 86 7.0 87 3.5 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023302784695312957 2 0.011488348105977753 3 0.001454856310363995 4 0.0 5 2.50837294890344E-4 6 0.0 7 0.0 8 1.003349179561376E-4 9 3.0100475386841274E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0020568658181008208 26-27 0.009255896181453692 28-29 2.7592102437937833E-4 30-31 0.0 32-33 3.0100475386841274E-4 34-35 0.00989553128342407 36-37 0.0011538515564955823 38-39 0.012780160174663026 40-41 0.012391362367582991 42-43 0.004966578438828811 44-45 6.89802560948446E-4 46-47 1.128767827006548E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.25418647445172E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 7.525118846710319E-5 66-67 6.647188314594115E-4 68-69 0.0011413096917510651 70-71 1.25418647445172E-5 72-73 2.006698359122752E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.8812797116775798E-4 82-83 0.0011287678270065478 84-85 0.00573163218824436 86-87 0.003975771124011952 88-89 0.0 90-91 0.001329437662918823 92-93 4.389652660581019E-4 94-95 1.25418647445172E-5 96-97 1.25418647445172E-5 98-99 0.0 100-101 2.50837294890344E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3986648.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.04913305349279 #Duplication Level Percentage of deduplicated Percentage of total 1 71.20061906687903 32.78726780901768 2 14.730751776218225 13.566766970420966 3 5.679024560990376 7.845424728692979 4 2.6776447984681555 4.93212886378612 5 1.4658555299340386 3.3750688167565364 6 0.9355455471007101 2.5848636825625984 7 0.6172959738200002 1.9898161102275813 8 0.42672197872425766 1.5720141740098448 9 0.33049405177032887 1.3697068107023826 >10 1.695715181165814 14.257828355460367 >50 0.14149170449365794 4.514270895213449 >100 0.09129851383479601 7.7953201442893 >500 0.005628663883829767 1.75595866073525 >1k 0.0018033582758919648 1.363869433669557 >5k 1.0929444096314912E-4 0.28969454445558723 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6350 0.15928168225536843 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5162 0.12948221162239557 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 1.2541864744517198E-5 16-17 0.0 0.0 0.0 0.0 2.5083729489034395E-5 18-19 0.0 0.0 0.0 2.5083729489034395E-5 2.5083729489034395E-5 20-21 0.0 0.0 0.0 6.2709323722586E-5 2.5083729489034395E-5 22-23 0.0 0.0 0.0 2.0066983591227516E-4 3.762559423355159E-5 24-25 0.0 0.0 0.0 3.6371407759099874E-4 5.016745897806879E-5 26-27 0.0 0.0 0.0 6.396351019703772E-4 5.016745897806879E-5 28-29 0.0 0.0 0.0 0.002408038030947302 5.016745897806879E-5 30-31 0.0 0.0 0.0 0.009243354316709175 5.016745897806879E-5 32-33 0.0 0.0 0.0 0.024419010657574985 5.016745897806879E-5 34-35 0.0 0.0 0.0 0.047997716377267324 5.016745897806879E-5 36-37 0.0 0.0 0.0 0.09061497277913677 5.016745897806879E-5 38-39 0.0 0.0 0.0 0.15346225701391242 6.2709323722586E-5 40-41 0.0 0.0 0.0 0.22433633468517913 7.525118846710319E-5 42-43 0.0 0.0 0.0 0.29729236190403563 7.525118846710319E-5 44-45 0.0 0.0 0.0 0.38809546265434014 7.525118846710319E-5 46-47 0.0 0.0 0.0 0.49672055320660363 1.0033491795613758E-4 48-49 0.0 0.0 0.0 0.612256211233096 1.25418647445172E-4 50-51 0.0 0.0 0.0 0.7373989376538888 1.25418647445172E-4 52-53 0.0 0.0 0.0 0.861300019464974 1.505023769342064E-4 54-55 0.0 0.0 0.0 1.000853348477217 1.7558610642324078E-4 56-57 0.0 0.0 0.0 1.1630698270828024 1.7558610642324078E-4 58-59 0.0 0.0 0.0 1.3362353536103515 1.7558610642324078E-4 60-61 0.0 0.0 0.0 1.5248022900441676 1.7558610642324078E-4 62-63 0.0 0.0 0.0 1.71852393288798 1.7558610642324078E-4 64-65 0.0 0.0 0.0 1.917488075195001 1.8812797116775798E-4 66-67 0.0 0.0 0.0 2.150942345549444 2.2575356540130957E-4 68-69 0.0 0.0 0.0 2.41055894576095 2.759210243793784E-4 70-71 0.0 0.0 0.0 2.68812295442186 3.6371407759099874E-4 72-73 0.0 0.0 0.0 2.969198685211235 4.5150713080261914E-4 74-75 0.0 0.0 0.0 3.2631047436342513 4.7659086029165355E-4 76-77 0.0 0.0 0.0 3.5737792752206863 4.7659086029165355E-4 78-79 0.0 0.0 0.0 3.9224681988477537 4.7659086029165355E-4 80-81 0.0 0.0 0.0 4.296692359094658 5.01674589780688E-4 82-83 0.0 2.5083729489034395E-5 0.0 4.679093815154987 5.267583192697223E-4 84-85 0.0 2.5083729489034395E-5 0.0 5.065546293527795 5.267583192697223E-4 86-87 0.0 2.5083729489034395E-5 0.0 5.465406025312493 5.267583192697223E-4 88-89 0.0 2.5083729489034395E-5 0.0 5.896143828098191 5.267583192697223E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5395 0.0 41.482033 1 GTATCAA 8325 0.0 39.495937 1 ATCAACG 10705 0.0 29.993874 3 TATCAAC 11000 0.0 29.451527 2 TCAACGC 10980 0.0 29.28592 4 CAACGCA 11125 0.0 28.776136 5 AACGCAG 11550 0.0 27.758396 6 TACATGG 11075 0.0 24.019306 2 GTACATG 11525 0.0 23.747183 1 CGCAGAG 13530 0.0 23.450453 8 GGACCGA 1420 0.0 23.414358 6 ACGCAGA 13715 0.0 23.376556 7 ACATGGG 11525 0.0 22.254887 3 GCAGAGT 15330 0.0 20.015337 9 AGGACCG 1615 0.0 19.999031 5 GCACCGT 1175 0.0 19.403315 6 AGAGTAC 13360 0.0 19.304827 10-11 ACCGAGT 1565 0.0 19.120478 8 GAGTACT 7765 0.0 19.115301 12-13 TAAGGTG 1640 0.0 19.114925 5 >>END_MODULE