FastQCFastQC Report
Thu 26 May 2016
SRR938387_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938387_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3395231
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76260.22460916503177544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58680.17283065570501682No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA48170.14187547180147683No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA47040.13854727410299916No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA43480.12806197869894567No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA41590.12249534715016445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40690.11984457022217339No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA38860.11445465713525824No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC35690.1051180317333342No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG34590.10187819326578956No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54900.042.621981
GTATCAA95100.038.508011
ATCAACG116500.031.0674783
TATCAAC118900.030.6803842
TCAACGC118850.030.5332224
CAACGCA122200.029.5794225
AACGCAG127900.028.3514026
ACGCAGA144400.024.7815887
CGCAGAG145400.024.5784688
TAAGGTG21500.024.1121165
GTACATG97200.022.8502791
TACATGG92900.022.6283972
GTATAGG8350.021.6440221
GCAGAGT160100.021.5790169
GTAAGGT22000.021.4022674
ACATGGG95350.021.1491243
AGAGTAC134850.020.91517610-11
GAGTACT96600.020.29114712-13
GGTAAGG22500.020.0811393
GGACCGA9950.019.5830966
Produced by FastQC (version 0.11.5)