##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938387_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3395231 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.041398950469056 31.0 31.0 34.0 28.0 34.0 2 31.095116944914793 31.0 31.0 34.0 27.0 34.0 3 31.144460862898576 31.0 31.0 34.0 27.0 34.0 4 34.79068022175811 37.0 35.0 37.0 32.0 37.0 5 34.76066370741785 37.0 35.0 37.0 32.0 37.0 6 34.776173403223524 37.0 35.0 37.0 32.0 37.0 7 34.67576374037584 37.0 35.0 37.0 30.0 37.0 8 34.6362542047949 37.0 35.0 37.0 30.0 37.0 9 36.124201858430254 39.0 35.0 39.0 31.0 39.0 10-11 35.95177117551059 38.5 35.0 39.0 30.0 39.0 12-13 35.807163636288664 38.0 35.0 39.0 30.0 39.0 14-15 36.756648074902714 39.0 36.0 41.0 30.0 41.0 16-17 36.56200800475726 39.0 36.0 40.5 30.0 41.0 18-19 36.712265233205045 39.0 36.0 41.0 30.0 41.0 20-21 36.68314482873183 39.0 36.0 41.0 30.0 41.0 22-23 36.58868130032978 39.0 36.0 41.0 30.0 41.0 24-25 36.44167274627264 39.0 36.0 41.0 29.5 41.0 26-27 36.22062445824747 39.0 35.0 41.0 28.5 41.0 28-29 35.99928620467945 39.0 35.0 40.0 27.0 41.0 30-31 35.73083819628178 38.5 35.0 40.0 27.0 41.0 32-33 35.445931219407456 38.0 34.0 40.0 26.5 41.0 34-35 35.18142152919786 38.0 34.0 40.0 25.5 41.0 36-37 34.87997915311212 38.0 34.0 40.0 25.0 41.0 38-39 34.716121230042965 38.0 33.5 40.0 24.5 41.0 40-41 34.596256484463055 38.0 33.0 40.0 24.0 41.0 42-43 34.280699310297294 38.0 33.0 40.0 23.5 41.0 44-45 33.92854123916753 38.0 33.0 40.0 22.5 41.0 46-47 33.537013534572466 38.0 32.5 40.0 19.5 41.0 48-49 33.155103732264465 37.5 31.5 40.0 17.5 41.0 50-51 32.462050888437346 36.5 30.5 39.5 15.0 40.5 52-53 32.48152732465037 37.0 31.0 39.0 15.0 40.0 54-55 32.55977899589159 37.0 31.0 40.0 13.5 41.0 56-57 32.210070831704826 36.5 31.0 39.5 8.5 41.0 58-59 31.802924454919268 36.0 30.5 39.0 7.5 41.0 60-61 31.299228682820107 35.5 29.5 39.0 4.5 40.0 62-63 30.703898055831843 35.0 29.0 39.0 2.0 40.0 64-65 30.15266442842917 35.0 28.0 38.0 2.0 40.0 66-67 29.58194979958654 34.0 28.0 38.0 2.0 40.0 68-69 28.95704577980114 34.0 26.5 37.0 2.0 39.5 70-71 28.286039005887964 34.0 26.0 36.5 2.0 39.0 72-73 27.62127598976329 33.0 25.0 36.0 2.0 39.0 74-75 26.916018379898155 32.5 23.5 35.5 2.0 38.5 76-77 26.13028745319538 32.0 21.0 35.0 2.0 37.5 78-79 25.3100868541787 31.0 18.5 35.0 2.0 37.0 80-81 24.589575201216057 31.0 15.0 34.5 2.0 36.0 82-83 23.80952680391997 30.5 7.5 34.0 2.0 36.0 84-85 23.065298060721055 30.0 3.5 34.0 2.0 35.0 86-87 22.275136802179293 29.0 2.0 34.0 2.0 35.0 88-89 21.403591684925118 29.0 2.0 34.0 2.0 35.0 90-91 20.44987189384169 27.5 2.0 33.5 2.0 35.0 92-93 19.466645126649702 26.0 2.0 33.0 2.0 35.0 94-95 18.419447601650667 24.5 2.0 32.5 2.0 34.0 96-97 17.145694652293173 20.5 2.0 32.0 2.0 34.0 98-99 15.612364077731382 2.0 2.0 32.0 2.0 34.0 100-101 12.23387495578357 2.0 2.0 27.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5008499344923649 1101 2 0.4031259203308757 1101 3 0.37091107708149096 1101 4 0.3738170963738128 1101 5 0.32946143700757347 1101 6 0.5057233959641039 1101 7 0.4408308088388537 1101 8 0.5007235399273711 1101 9 0.6317569360294897 1101 10-11 0.6674232383297181 1101 12-13 0.6558544552302479 1101 14-15 0.8473138289358744 1101 16-17 0.6672188169396236 1101 18-19 0.7837127943500022 1101 20-21 0.6866784070825531 1101 22-23 0.804651094458535 1101 24-25 0.7300819010912178 1101 26-27 0.8434807201368386 1101 28-29 0.7864509098591057 1101 30-31 0.9026342239510754 1101 32-33 0.96799753528785 1101 34-35 0.7984883180930069 1101 36-37 0.841890550255151 1101 38-39 0.8960694357679202 1101 40-41 0.967320409203893 1101 42-43 1.0232790728972745 1101 44-45 1.0563613024832748 1101 46-47 0.8460994492269762 1101 48-49 0.8532418775861075 1101 50-51 0.9264558580649052 1101 52-53 0.9439158502444442 1101 54-55 0.9324677180598684 1101 56-57 0.9021941734397316 1101 58-59 0.770790419368705 1101 60-61 0.841511190662473 1101 62-63 0.7889715127692263 1101 64-65 0.6055774439278316 1101 66-67 0.5662919337642904 1101 68-69 0.6357965388842537 1101 70-71 0.8488811768447597 1101 72-73 0.8929075384002196 1101 74-75 0.7553467393519853 1101 76-77 0.7887506725901794 1101 78-79 1.0863207885148647 1101 80-81 1.1252710274431195 1101 82-83 1.1833228071585928 1101 84-85 1.1967827955304209 1101 86-87 1.1808977029344625 1101 88-89 1.0674721961453422 1101 90-91 0.8444735170514193 1101 92-93 0.938618576738417 1101 94-95 0.8836766932265085 1101 96-97 0.9372582362911324 1101 98-99 1.3043263118617165 1101 100-101 1.1838421514622102 1102 1 0.3846957731131546 1102 2 0.46684638678906865 1102 3 0.3481227347912892 1102 4 0.32439311116957015 1102 5 0.2640650685367092 1102 6 0.3573126220819631 1102 7 0.4010715280003154 1102 8 0.412548911945926 1102 9 0.44570696577368807 1102 10-11 0.5751889411786806 1102 12-13 0.5664970811893753 1102 14-15 0.6245861273631022 1102 16-17 0.6835026860766718 1102 18-19 0.6575426517650911 1102 20-21 0.6397597225396012 1102 22-23 0.682106251296176 1102 24-25 0.6682279191854903 1102 26-27 0.5064235702217417 1102 28-29 0.3667808652212017 1102 30-31 0.5587273557056349 1102 32-33 0.7509232122384688 1102 34-35 0.5442590421662246 1102 36-37 0.6587448706541963 1102 38-39 0.6619267673170839 1102 40-41 0.8150373077984909 1102 42-43 0.8447976691438868 1102 44-45 0.7747464769081631 1102 46-47 0.7975001813808191 1102 48-49 0.8816213012256142 1102 50-51 0.9136121072996914 1102 52-53 0.7368944185761563 1102 54-55 0.6908770263379793 1102 56-57 0.8306737766967487 1102 58-59 0.8471564919675991 1102 60-61 0.9282035600148575 1102 62-63 0.9348849081740696 1102 64-65 0.9024189772017301 1102 66-67 0.7732002042362929 1102 68-69 0.7598678428613326 1102 70-71 0.9637417716135275 1102 72-73 0.9649430571520305 1102 74-75 1.0845216762985999 1102 76-77 0.9802597478120383 1102 78-79 1.1434932098079642 1102 80-81 1.3088505366422183 1102 82-83 1.2686679551089775 1102 84-85 1.279432420378999 1102 86-87 1.416793154592579 1102 88-89 1.44110913037537 1102 90-91 1.3504087632800967 1102 92-93 1.473365545782844 1102 94-95 1.5090219384036985 1102 96-97 1.6258043246767784 1102 98-99 1.7024532374925752 1102 100-101 1.448005305960642 1103 1 0.4917232697990883 1103 2 0.40222325391123803 1103 3 0.39417889456132826 1103 4 0.3697405898712063 1103 5 0.3455943476946146 1103 6 0.4065038257515141 1103 7 0.37536429010548744 1103 8 0.35451092343166835 1103 9 0.5009301990022266 1103 10-11 0.6552096461415005 1103 12-13 0.7377250483760491 1103 14-15 0.8031275401663862 1103 16-17 0.7683766399066272 1103 18-19 0.738149302641439 1103 20-21 0.7148921675058517 1103 22-23 0.852121516671815 1103 24-25 0.8086690262801355 1103 26-27 0.7782863855420885 1103 28-29 0.8770726112624132 1103 30-31 1.007791002014919 1103 32-33 1.0036427878285892 1103 34-35 1.0072075007260324 1103 36-37 0.9922877039588442 1103 38-39 1.014218736243599 1103 40-41 1.0680497473443467 1103 42-43 1.0885429286892858 1103 44-45 1.077414246490811 1103 46-47 0.8442504160818274 1103 48-49 0.9944005204329542 1103 50-51 1.2430010423178217 1103 52-53 1.2072669369934061 1103 54-55 1.1942683534490044 1103 56-57 1.2109527617698888 1103 58-59 1.2010817322625407 1103 60-61 1.365183047417041 1103 62-63 1.2157576174739155 1103 64-65 1.4288039834740829 1103 66-67 1.3072134946877512 1103 68-69 1.5098054070738378 1103 70-71 1.5888970544746783 1103 72-73 1.7220107708563894 1103 74-75 1.6488172392941323 1103 76-77 1.6810202348879137 1103 78-79 1.7596184553000747 1103 80-81 1.8547632946629058 1103 82-83 1.7876078861261533 1103 84-85 2.0014102187012917 1103 86-87 1.9187342884578698 1103 88-89 1.8602466322916094 1103 90-91 1.7710731079633177 1103 92-93 1.7495935469778452 1103 94-95 1.8715023203991024 1103 96-97 2.1366129535816505 1103 98-99 2.214559894211371 1103 100-101 1.828988524860339 1104 1 0.37395131538139026 1104 2 0.25888079531262775 1104 3 0.38051125833522903 1104 4 0.224643618918968 1104 5 0.3190025042009239 1104 6 0.43936228508254516 1104 7 0.34828741674956376 1104 8 0.5569789975104129 1104 9 0.5689014183958179 1104 10-11 0.7360825598126937 1104 12-13 0.6407612252501096 1104 14-15 0.7882300693257065 1104 16-17 0.8513927902253613 1104 18-19 0.8467545928939089 1104 20-21 0.9183875843613762 1104 22-23 0.9512954900544557 1104 24-25 0.9081101538107177 1104 26-27 0.9189511741250982 1104 28-29 0.9527679124512005 1104 30-31 0.9258504723292944 1104 32-33 0.9126769255260427 1104 34-35 0.8617293098098813 1104 36-37 1.0295892269376026 1104 38-39 1.2207880517386371 1104 40-41 1.027842422718038 1104 42-43 1.1010195026724645 1104 44-45 0.9857124595597426 1104 46-47 1.1070943270184372 1104 48-49 1.0170706010694843 1104 50-51 1.0502529516695134 1104 52-53 1.2032805118260868 1104 54-55 1.269212471204952 1104 56-57 1.3597000889776893 1104 58-59 1.4172953625272022 1104 60-61 1.2869844268129498 1104 62-63 1.3239735580716392 1104 64-65 1.4454867344213795 1104 66-67 1.3077331695401426 1104 68-69 1.3960946709791102 1104 70-71 1.5837199422809398 1104 72-73 1.5121304198938823 1104 74-75 1.4102517119848734 1104 76-77 1.5080091574962609 1104 78-79 1.5352846396884665 1104 80-81 1.6102029333344205 1104 82-83 1.5631296121620757 1104 84-85 1.5404090960535264 1104 86-87 1.6282618108382856 1104 88-89 1.705915583276326 1104 90-91 1.544236723369405 1104 92-93 1.7545373995807978 1104 94-95 1.7343694447298397 1104 96-97 1.882126489892105 1104 98-99 1.9779492448666982 1104 100-101 1.7993275354694607 1105 1 0.40103497191257276 1105 2 0.35933327230094037 1105 3 0.3989542203788723 1105 4 0.34395178160383466 1105 5 0.30102139188320365 1105 6 0.4283296033160724 1105 7 0.3991141568467782 1105 8 0.2709737875610898 1105 9 0.5570410269385775 1105 10-11 0.5213867536279793 1105 12-13 0.5607152988357953 1105 14-15 0.66843904905474 1105 16-17 0.6340204210558511 1105 18-19 0.5813482362386182 1105 20-21 0.5884565547823115 1105 22-23 0.6166069881411218 1105 24-25 0.7734896850445594 1105 26-27 0.6815870788205274 1105 28-29 0.54658497600483 1105 30-31 0.8039497781003107 1105 32-33 0.7382677311936376 1105 34-35 0.923036386549974 1105 36-37 0.9345241348908573 1105 38-39 0.9542564817727666 1105 40-41 1.0876487302472526 1105 42-43 1.0319792710042535 1105 44-45 1.130408944459944 1105 46-47 1.0685749610033426 1105 48-49 0.9532267444142022 1105 50-51 1.1522149242106252 1105 52-53 1.1685567813472346 1105 54-55 1.248380592261931 1105 56-57 1.1270837700719056 1105 58-59 1.1981436276011515 1105 60-61 1.3275040643142475 1105 62-63 1.4601369733679483 1105 64-65 1.5626745576192285 1105 66-67 1.6525778406540113 1105 68-69 1.609878574433452 1105 70-71 1.5398139993188984 1105 72-73 1.6302930078036972 1105 74-75 1.6365585624864778 1105 76-77 1.637106659823285 1105 78-79 1.7453879660407274 1105 80-81 1.7494675935888395 1105 82-83 1.636721923931649 1105 84-85 1.7840403208440918 1105 86-87 1.8633153647218315 1105 88-89 1.6322015875166862 1105 90-91 1.5057398196047984 1105 92-93 1.6781560519750478 1105 94-95 1.7030963360836502 1105 96-97 1.8369284019305745 1105 98-99 2.0502833474109163 1105 100-101 1.4134815004606711 1106 1 0.25205456594843767 1106 2 0.18648067009927516 1106 3 0.16418368960208696 1106 4 0.11804395044977412 1106 5 0.2251574906480016 1106 6 0.0876233652675964 1106 7 0.3141643532393559 1106 8 0.2072435883259871 1106 9 0.11313840277195908 1106 10-11 0.11900904676974733 1106 12-13 0.06731584989786654 1106 14-15 0.34534144065120387 1106 16-17 0.1952457266271992 1106 18-19 0.003487058012360933 1106 20-21 0.22419241322460692 1106 22-23 0.23922847374761602 1106 24-25 0.10106580331940052 1106 26-27 0.19517038738943882 1106 28-29 0.4492483080891745 1106 30-31 0.18599964178835648 1106 32-33 0.2967843211982881 1106 34-35 -0.04691183428830925 1106 36-37 0.06815814052422553 1106 38-39 0.17918897182392612 1106 40-41 0.3324925367747227 1106 42-43 -0.014034734887523825 1106 44-45 0.10201883062976691 1106 46-47 0.15817616778405608 1106 48-49 0.6681890809690572 1106 50-51 0.28730612724437066 1106 52-53 0.09274504689067697 1106 54-55 0.2779776483113423 1106 56-57 -0.4510317817653977 1106 58-59 -0.1708106788229493 1106 60-61 0.5340891914526011 1106 62-63 0.43309738152857946 1106 64-65 0.6414078739404836 1106 66-67 0.11733425571421918 1106 68-69 0.20671424746564782 1106 70-71 0.1682070202760535 1106 72-73 0.4964158532995917 1106 74-75 0.8088588913148413 1106 76-77 0.7775405969776621 1106 78-79 0.9802197887447441 1106 80-81 0.8934905405111593 1106 82-83 0.7556459477830124 1106 84-85 0.7231239782682515 1106 86-87 0.8027058855497806 1106 88-89 0.7253196104119262 1106 90-91 0.8938659017020605 1106 92-93 0.7882408754801382 1106 94-95 0.6583914256231616 1106 96-97 0.738707321383778 1106 98-99 0.9558651119723915 1106 100-101 0.6251976259710315 1107 1 0.3461515228326917 1107 2 0.1773308152984825 1107 3 0.23759309116060123 1107 4 0.1722525951725089 1107 5 0.31153651623761647 1107 6 0.3014793300372318 1107 7 0.34264164769570016 1107 8 0.24636004464377237 1107 9 0.3384585355882308 1107 10-11 0.5423279986763703 1107 12-13 0.554317298911009 1107 14-15 0.5489799970063984 1107 16-17 0.7726847550326852 1107 18-19 0.7178700578050865 1107 20-21 0.6390650076229107 1107 22-23 0.6588666469186464 1107 24-25 0.7688350913274604 1107 26-27 0.6765781941241329 1107 28-29 0.8048064540923789 1107 30-31 0.8014055739936126 1107 32-33 0.820134175560085 1107 34-35 0.5789176794848387 1107 36-37 0.6336154513928918 1107 38-39 0.9045977669354812 1107 40-41 0.694348371098684 1107 42-43 0.9138037206858414 1107 44-45 0.9063191165754745 1107 46-47 0.768243077635745 1107 48-49 0.8463355419872798 1107 50-51 0.74272921807313 1107 52-53 0.7605041438303104 1107 54-55 0.8345666130461709 1107 56-57 0.9326428164791665 1107 58-59 0.7639203098956919 1107 60-61 0.6647563270129382 1107 62-63 0.9330106790541528 1107 64-65 0.93370551146268 1107 66-67 1.001393997952313 1107 68-69 1.0728133291092767 1107 70-71 1.1224415973482813 1107 72-73 1.266788034254084 1107 74-75 1.1503242791539776 1107 76-77 1.3082403282783837 1107 78-79 1.4606850784360539 1107 80-81 1.563683107804355 1107 82-83 1.4529491872571292 1107 84-85 1.627006602872875 1107 86-87 1.7983346227321597 1107 88-89 1.6992926407684514 1107 90-91 1.6078742621410278 1107 92-93 1.7209702599678245 1107 94-95 1.644191034097993 1107 96-97 1.7713501758612793 1107 98-99 1.7766092355671557 1107 100-101 1.582036804953475 1108 1 0.37701588346608617 1108 2 0.2232852384984163 1108 3 0.2977287492432197 1108 4 0.3264626527187531 1108 5 0.23022588873365635 1108 6 0.34893708964212067 1108 7 0.3871246618108799 1108 8 0.41174411379174813 1108 9 0.4985234030953549 1108 10-11 0.49759974971372856 1108 12-13 0.45483699861804894 1108 14-15 0.5830318601857272 1108 16-17 0.6492867633712223 1108 18-19 0.7076095528910287 1108 20-21 0.6279124643916631 1108 22-23 0.5830984370614658 1108 24-25 0.630827323080176 1108 26-27 0.722393898622478 1108 28-29 0.6604864344515704 1108 30-31 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-3.3162953335714747 2316 90-91 -3.2001232070260386 2316 92-93 -3.2876105556817983 2316 94-95 -3.628814300649328 2316 96-97 -3.5295543233680817 2316 98-99 -3.544770692745484 2316 100-101 -2.946500167755536 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14195.0 3 4996.0 4 5398.0 5 6090.0 6 7366.0 7 8848.0 8 11026.0 9 14061.0 10 17087.0 11 21374.0 12 25550.0 13 29812.0 14 33601.0 15 37567.0 16 42726.0 17 47493.0 18 47104.0 19 46247.0 20 48865.0 21 52929.0 22 58540.0 23 64838.0 24 71415.0 25 79530.0 26 88081.0 27 99026.0 28 112156.0 29 127431.0 30 144629.0 31 166997.0 32 195494.0 33 229038.0 34 273714.0 35 325836.0 36 366011.0 37 329348.0 38 135062.0 39 5750.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.035097413614345 18.35014821761834 14.661850575904387 23.952903792862934 2 15.468537291358484 19.408271741043773 40.40411932928068 24.719071638317065 3 19.5089931425353 22.953796523054557 29.33075213899525 28.20645819541489 4 12.921886740373854 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26.74622586224189 27.37659519849469 23.057104972746735 50-51 23.006513961887677 26.90299102619974 27.371438392772063 22.719056619140524 52-53 23.23696260094493 26.551430641365464 27.436739999620013 22.774866758069592 54-55 22.65774378585086 26.765991328484535 27.71553376008493 22.860731125579676 56-57 22.80486969636938 26.93795489543527 27.40719083680508 22.849984571390276 58-59 22.8169311583241 26.505693476415814 27.427832501939164 23.249542863320922 60-61 22.422337548855506 26.77023280506364 27.72360476861275 23.08382487746811 62-63 22.612649380400608 26.764658275142867 27.825791365036295 22.796900979420233 64-65 22.29508669949839 26.83765321202149 27.653792305900023 23.213467782580096 66-67 22.23357764428161 26.836618010671447 27.62651943205805 23.303284912988893 68-69 22.8395200830212 26.37191253493767 27.78361759935171 23.004949782689412 70-71 22.670965337363892 26.478647701249752 27.377715119520868 23.472671841865488 72-73 22.635261792479582 26.643055707162823 27.449537613799357 23.272144886558237 74-75 22.660703447706933 26.967773869080236 27.2819329786043 23.08958970460853 76-77 22.84148921236229 26.621246378011364 27.289484960617894 23.247779449008455 78-79 22.8270687277718 26.89504790739548 27.260978848699697 23.01690451613302 80-81 23.09135814105557 26.87815625418809 26.9692301574795 23.061255447276842 82-83 22.984989779147103 26.994421240522882 27.048736089919938 22.971852890410073 84-85 22.956054407622418 27.03843755550378 27.012075560840394 22.993432476033412 86-87 23.153259690788506 27.040165032197688 26.695690071512285 23.110885205501525 88-89 23.004221938078338 27.140563267856976 26.54038420711997 23.314830586944712 90-91 22.937529713152337 27.12579696070269 26.60146064590114 23.33521268024383 92-93 23.120553898280114 26.726947298641036 26.87194785106618 23.280550952012668 94-95 22.893439084368524 26.586125554552005 27.00896270964681 23.511472651432662 96-97 23.18555394324174 26.249740878136922 26.967414514095122 23.597290664526213 98-99 22.962122983158487 26.512762790963514 26.55549668167877 23.96961754419923 100-101 23.618508230742282 25.835981165230752 26.909331328551588 23.636179275475374 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 263.0 1 201.0 2 202.0 3 241.5 4 218.0 5 235.0 6 408.5 7 910.5 8 1493.5 9 1742.5 10 1562.0 11 1365.0 12 1433.0 13 1621.5 14 1826.0 15 2033.0 16 2353.0 17 2753.0 18 3126.0 19 3641.5 20 4296.0 21 5160.0 22 6335.0 23 7898.5 24 10182.5 25 13335.5 26 17489.5 27 22273.5 28 26558.0 29 31405.5 30 37196.0 31 45337.5 32 56559.5 33 61195.0 34 61490.0 35 65484.0 36 71428.5 37 78250.5 38 84188.5 39 88730.0 40 94505.5 41 101684.0 42 106234.0 43 109381.0 44 112967.0 45 119371.5 46 124131.0 47 127581.0 48 130354.5 49 128438.0 50 124951.0 51 121521.0 52 119118.5 53 119061.5 54 120166.5 55 115298.0 56 106893.0 57 99156.0 58 92322.0 59 81986.5 60 66544.5 61 54967.5 62 47090.5 63 36898.5 64 27148.5 65 20782.5 66 15713.5 67 11937.5 68 9267.0 69 7109.5 70 5414.0 71 3922.0 72 2818.5 73 2178.5 74 1725.5 75 1291.5 76 929.0 77 658.5 78 463.0 79 323.0 80 221.5 81 142.5 82 76.0 83 48.5 84 41.5 85 30.5 86 18.5 87 13.5 88 10.0 89 7.5 90 4.5 91 5.0 92 4.5 93 2.5 94 2.0 95 2.0 96 2.0 97 0.5 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006568036166022283 2 0.1092120094332315 3 0.11887261868191001 4 0.12237753484225375 5 0.16258098491678474 6 0.10164256864996814 7 0.029335264669767682 8 0.029099640053946257 9 0.025977613894312346 10-11 0.0555632297183903 12-13 0.057065336644251896 14-15 0.030719559287718568 16-17 0.03506388814192613 18-19 0.040807238152573415 20-21 0.0632652093480532 22-23 0.035682402758457375 24-25 0.05412002894648406 26-27 0.0616894697297474 28-29 0.057978382030559925 30-31 0.07255765513451073 32-33 0.05484162933243718 34-35 0.06693211743177416 36-37 0.05731568779856216 38-39 0.035549863912057826 40-41 0.04048325430581896 42-43 0.03609474583614487 44-45 0.007657800014196383 46-47 0.014166930026263309 48-49 0.009321898863435213 50-51 0.008615025015970931 52-53 0.010971271174185202 54-55 0.012900447716223139 56-57 0.016596808876921776 58-59 0.021559652347660586 60-61 0.02681702658817618 62-63 0.012267206561203054 64-65 0.010131858480321368 66-67 0.011103810020584755 68-69 0.012826815023778942 70-71 0.034121389678640424 72-73 0.030763738903185088 74-75 0.03213330698264713 76-77 0.02531491966231458 78-79 0.005640264241225413 80-81 0.005478272317848182 82-83 0.006214599242290142 84-85 0.005654990779714252 86-87 0.01471181195035036 88-89 0.04154356507701538 90-91 0.038922241226002 92-93 0.03257510313731231 94-95 0.05083601086347292 96-97 0.04587316739273411 98-99 0.028554758129859203 100-101 0.07880170745377854 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3395231.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.084612730254854 #Duplication Level Percentage of deduplicated Percentage of total 1 75.9187746457197 41.0603752566924 2 13.173659008655713 14.249844914471547 3 4.632732490153427 7.516786278384519 4 2.108309698442216 4.561084542227505 5 1.1310346821006545 3.0585786382950406 6 0.71776730116687 2.329209990843022 7 0.46368447953286007 1.7554736853195165 8 0.32203852321740095 1.3933863049949053 9 0.23476385961661855 1.1427401187382258 >10 1.177633859064719 11.43372542375561 >50 0.0745415813562431 2.7673359525546855 >100 0.039152915499382275 3.9711571252173212 >500 0.002844089676969011 1.052346049722898 >1k 0.0029534777329624927 3.3087889566287045 >5k 1.0938806418379603E-4 0.3991667621541079 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7626 0.22460916503177544 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5868 0.17283065570501682 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4817 0.14187547180147683 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4704 0.13854727410299916 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4348 0.12806197869894567 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4159 0.12249534715016445 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4069 0.11984457022217339 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3886 0.11445465713525824 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3569 0.1051180317333342 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3459 0.10187819326578956 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1781230791071358E-4 2 0.0 0.0 0.0 0.0 1.1781230791071358E-4 3 0.0 0.0 0.0 0.0 1.1781230791071358E-4 4 0.0 0.0 0.0 2.9453076977678396E-5 1.1781230791071358E-4 5 0.0 0.0 0.0 2.9453076977678396E-5 1.1781230791071358E-4 6 0.0 0.0 0.0 2.9453076977678396E-5 1.1781230791071358E-4 7 0.0 0.0 0.0 2.9453076977678396E-5 1.1781230791071358E-4 8 0.0 0.0 0.0 2.9453076977678396E-5 1.1781230791071358E-4 9 0.0 0.0 0.0 2.9453076977678396E-5 1.1781230791071358E-4 10-11 2.9453076977678396E-5 0.0 0.0 2.9453076977678396E-5 1.1781230791071358E-4 12-13 5.890615395535679E-5 0.0 0.0 2.9453076977678396E-5 1.6199192337723118E-4 14-15 8.835923093303519E-5 0.0 0.0 7.363269244419599E-5 1.9144500035490956E-4 16-17 1.1781230791071358E-4 0.0 0.0 8.835923093303519E-5 2.503511543102664E-4 18-19 1.325388463995528E-4 0.0 0.0 8.835923093303519E-5 2.650776927991056E-4 20-21 1.4726538488839198E-4 0.0 0.0 1.1781230791071358E-4 2.9453076977678397E-4 22-23 1.4726538488839198E-4 0.0 0.0 2.2089807733258798E-4 3.0925730826562316E-4 24-25 1.7671846186607037E-4 0.0 0.0 4.2706961617633676E-4 3.2398384675446236E-4 26-27 1.9144500035490956E-4 0.0 0.0 6.774207704866031E-4 3.2398384675446236E-4 28-29 2.9453076977678397E-4 0.0 0.0 0.0022678869272812364 3.2398384675446236E-4 30-31 3.3871038524330155E-4 0.0 0.0 0.008939008862725393 3.3871038524330155E-4 32-33 3.5343692373214074E-4 0.0 0.0 0.023606641197609232 3.828900007098192E-4 34-35 3.6816346222097993E-4 0.0 0.0 0.045048481237359106 3.828900007098192E-4 36-37 4.1234307768749757E-4 0.0 0.0 0.08095178207314907 3.976165391986584E-4 38-39 4.1234307768749757E-4 0.0 0.0 0.13627938717571794 4.7124923164285434E-4 40-41 4.4179615466517595E-4 0.0 0.0 0.19580405574760598 5.448819240870504E-4 42-43 4.4179615466517595E-4 0.0 0.0 0.2604241066366324 5.596084625758896E-4 44-45 4.859757701316936E-4 0.0 0.0 0.34224475448062297 5.743350010647287E-4 46-47 5.15428847109372E-4 0.0 0.0 0.4389686592753188 6.037880780424071E-4 48-49 5.301553855982112E-4 0.0 0.0 0.5398012683084008 6.774207704866031E-4 50-51 5.301553855982112E-4 0.0 0.0 0.6528421777487305 7.068738474642815E-4 52-53 5.890615395535679E-4 0.0 0.0 0.7628052406448929 7.510534629307992E-4 54-55 6.479676935089247E-4 0.0 0.0 0.8826939904825327 7.657800014196384E-4 56-57 6.479676935089247E-4 0.0 0.0 1.0253794219008958 7.805065399084776E-4 58-59 6.479676935089247E-4 0.0 0.0 1.1765620660273188 7.952330783973167E-4 60-61 6.479676935089247E-4 0.0 0.0 1.3439880821069319 8.246861553749951E-4 62-63 6.479676935089247E-4 0.0 0.0 1.5126364008811182 8.541392323526735E-4 64-65 6.774207704866031E-4 0.0 0.0 1.6905329858262959 9.130453863080303E-4 66-67 7.068738474642815E-4 0.0 0.0 1.8994289342904798 9.277719247968695E-4 68-69 7.363269244419599E-4 0.0 0.0 2.1299434412562794 9.424984632857087E-4 70-71 7.363269244419599E-4 0.0 0.0 2.374816323248698 9.424984632857087E-4 72-73 7.363269244419599E-4 0.0 0.0 2.6271113806394912 0.001030857694218744 74-75 7.952330783973167E-4 0.0 0.0 2.882999713421561 0.0010897638481741007 76-77 7.952330783973167E-4 0.0 0.0 3.158577428163209 0.0011486700021294575 78-79 8.246861553749951E-4 0.0 0.0 3.4783053052943966 0.0011486700021294575 80-81 9.424984632857087E-4 0.0 0.0 3.8095935151393236 0.0011486700021294575 82-83 9.424984632857087E-4 0.0 0.0 4.149644015385109 0.0012370292330624926 84-85 9.424984632857087E-4 0.0 0.0 4.5007983256514805 0.0012370292330624926 86-87 9.424984632857087E-4 0.0 0.0 4.871538932108007 0.0012812088485290103 88-89 9.424984632857087E-4 1.4726538488839198E-5 0.0 5.2596568539813635 0.0013253884639955278 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5490 0.0 42.62198 1 GTATCAA 9510 0.0 38.50801 1 ATCAACG 11650 0.0 31.067478 3 TATCAAC 11890 0.0 30.680384 2 TCAACGC 11885 0.0 30.533222 4 CAACGCA 12220 0.0 29.579422 5 AACGCAG 12790 0.0 28.351402 6 ACGCAGA 14440 0.0 24.781588 7 CGCAGAG 14540 0.0 24.578468 8 TAAGGTG 2150 0.0 24.112116 5 GTACATG 9720 0.0 22.850279 1 TACATGG 9290 0.0 22.628397 2 GTATAGG 835 0.0 21.644022 1 GCAGAGT 16010 0.0 21.579016 9 GTAAGGT 2200 0.0 21.402267 4 ACATGGG 9535 0.0 21.149124 3 AGAGTAC 13485 0.0 20.915176 10-11 GAGTACT 9660 0.0 20.291147 12-13 GGTAAGG 2250 0.0 20.081139 3 GGACCGA 995 0.0 19.583096 6 >>END_MODULE