FastQCFastQC Report
Thu 26 May 2016
SRR938387_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938387_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3395231
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78330.23070595196615487No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65240.19215187420237387No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA46550.13710407333109292No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA46460.13683899563829383No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45070.13274501793839655No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA42870.1262653410033073No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA42380.12482214023140105No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC41860.12329058022856178No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG38490.11336489328708414No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA34070.10034663326295029No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59500.042.6567461
GTATCAA95950.037.0031781
ATCAACG121600.028.9852963
TATCAAC123450.028.6058432
TCAACGC124150.028.3899484
CAACGCA126400.027.8845885
AACGCAG131450.026.803066
GTAAGGT23900.025.2413884
TAAGGTG25300.024.2201395
GGTAAGG25100.023.466883
AGGTAAG24250.023.3234182
ACGCAGA150250.023.3229247
CGCAGAG150950.023.1518558
AAGGTAA24450.022.3553981
TACATGG91400.021.528372
GTACATG95250.021.2073971
ACATGGG90700.020.9488473
AGAGTAC138800.020.64566610-11
GAGTACT99950.020.30927512-13
GCAGAGT167950.020.2429669
Produced by FastQC (version 0.11.5)