##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938387_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3395231 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.799895795013654 31.0 31.0 34.0 30.0 34.0 2 32.02499712096173 31.0 31.0 34.0 30.0 34.0 3 32.090900736945436 33.0 31.0 34.0 30.0 34.0 4 35.756730838049016 37.0 35.0 37.0 35.0 37.0 5 35.65049859641362 37.0 35.0 37.0 33.0 37.0 6 35.65394990797386 37.0 35.0 37.0 33.0 37.0 7 35.594936839349074 37.0 35.0 37.0 33.0 37.0 8 35.566004492772365 37.0 35.0 37.0 33.0 37.0 9 37.16161197868422 39.0 37.0 39.0 33.0 39.0 10-11 37.055107443352156 39.0 37.0 39.0 33.0 39.0 12-13 36.945495166602804 39.0 37.0 39.0 33.0 39.0 14-15 37.996384929331754 40.0 37.0 41.0 33.0 41.0 16-17 37.899783402071904 40.0 37.0 41.0 33.0 41.0 18-19 38.138222553929324 40.0 37.0 41.0 33.0 41.0 20-21 38.101770542269435 40.0 37.0 41.0 33.0 41.0 22-23 37.99764390110717 40.0 37.0 41.0 33.0 41.0 24-25 37.86651364811408 40.0 37.0 41.0 32.0 41.0 26-27 37.67105905312481 40.0 37.0 41.0 32.0 41.0 28-29 37.55464090661283 39.5 37.0 41.0 32.0 41.0 30-31 37.301329865331695 39.0 36.0 41.0 31.0 41.0 32-33 37.09705274839915 39.0 36.0 41.0 31.0 41.0 34-35 37.00185200948036 39.0 36.0 41.0 31.0 41.0 36-37 36.947930347007315 39.0 36.0 41.0 30.5 41.0 38-39 36.77659767479739 39.0 35.0 41.0 30.0 41.0 40-41 36.60203606175839 39.0 35.0 40.5 30.0 41.0 42-43 36.38782839812667 39.0 35.0 40.0 30.0 41.0 44-45 36.09544313774232 38.5 35.0 40.0 29.5 41.0 46-47 35.72589979886494 38.0 35.0 40.0 28.5 41.0 48-49 35.36752418318518 38.0 34.0 40.0 27.0 41.0 50-51 34.98451195220591 38.0 34.0 40.0 26.0 41.0 52-53 34.47809515759016 38.0 33.0 40.0 24.5 41.0 54-55 34.13207952566408 37.5 33.0 40.0 24.0 41.0 56-57 33.78033070503892 37.0 33.0 40.0 23.0 41.0 58-59 33.279912029549685 36.5 32.0 39.0 22.5 40.5 60-61 32.865421380754356 36.0 31.0 39.0 21.5 40.0 62-63 32.366655611945106 35.5 31.0 39.0 19.5 40.0 64-65 31.8262745598164 35.0 30.5 38.0 18.0 40.0 66-67 31.302300491483496 35.0 29.5 38.0 15.5 40.0 68-69 30.77722531986778 34.0 29.0 37.5 12.0 39.5 70-71 30.166866260351654 34.0 28.5 37.0 8.0 39.0 72-73 29.649488503138667 34.0 28.0 36.0 7.0 39.0 74-75 29.039483469607813 33.5 27.0 36.0 6.0 38.5 76-77 27.83996184648408 31.5 26.0 34.5 2.0 36.5 78-79 28.51725803045507 33.0 27.0 35.0 2.0 37.0 80-81 28.115566068995008 33.0 26.0 35.0 2.0 36.5 82-83 27.64343280324667 33.0 26.0 35.0 2.0 36.0 84-85 27.057618170899122 32.0 25.5 35.0 2.0 36.0 86-87 26.420085555298005 32.0 24.5 34.5 2.0 35.0 88-89 25.625969190314297 31.0 22.0 34.0 2.0 35.0 90-91 24.976194697798178 31.0 19.5 34.0 2.0 35.0 92-93 24.108449911066433 31.0 15.5 34.0 2.0 35.0 94-95 23.2498177590862 30.0 4.5 34.0 2.0 35.0 96-97 22.134237699879627 29.5 2.0 34.0 2.0 35.0 98-99 20.69824851387137 29.0 2.0 34.0 2.0 35.0 100-101 16.747858540405645 21.0 2.0 31.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28316321150479595 1101 2 0.2704398099544676 1101 3 0.2974725911415632 1101 4 0.246386651353518 1101 5 0.122925364598089 1101 6 0.21937832249898293 1101 7 0.24291264694863912 1101 8 0.265418927982779 1101 9 0.28166052694759713 1101 10-11 0.28410825566961506 1101 12-13 0.38240742030756536 1101 14-15 0.5020286262689382 1101 16-17 0.51184042464687 1101 18-19 0.4248580036466123 1101 20-21 0.3878688967345596 1101 22-23 0.4262670948981011 1101 24-25 0.45087233510573554 1101 26-27 0.4060037257925444 1101 28-29 0.4384219907953195 1101 30-31 0.47904394192332234 1101 32-33 0.4594736047266892 1101 34-35 0.49408277238469367 1101 36-37 0.5209341183825273 1101 38-39 0.5831902881717355 1101 40-41 0.7210500006820908 1101 42-43 0.669532864038139 1101 44-45 0.6849749584835294 1101 46-47 0.6838092091794508 1101 48-49 0.8191467309692868 1101 50-51 0.7735100853508001 1101 52-53 0.6911610124383074 1101 54-55 0.8652794360967917 1101 56-57 0.7935095278249236 1101 58-59 0.8820295076847842 1101 60-61 0.9074712226508908 1101 62-63 1.123076455593285 1101 64-65 0.96251214512111 1101 66-67 0.7826829314562325 1101 68-69 1.0924089361584102 1101 70-71 1.095046020278435 1101 72-73 0.919647715138769 1101 74-75 0.989815452248628 1101 76-77 0.7946259576999921 1101 78-79 1.102839867736769 1101 80-81 1.1478762624174657 1101 82-83 1.0630306016229518 1101 84-85 1.173205618904408 1101 86-87 1.2096887231496538 1101 88-89 1.4163220082030286 1101 90-91 1.4823115149219817 1101 92-93 1.7329503393169787 1101 94-95 1.609970108431689 1101 96-97 1.4470736881008115 1101 98-99 1.5316361964455574 1101 100-101 1.9474449950262347 1102 1 0.30995819834677363 1102 2 0.28762572776828677 1102 3 0.24171644028589867 1102 4 0.18268707065310963 1102 5 0.10655795242990251 1102 6 0.053892361329204164 1102 7 0.15473585837156634 1102 8 0.19336144317536963 1102 9 0.1927520166494716 1102 10-11 0.3702950456438714 1102 12-13 0.29973862017273945 1102 14-15 0.5386166028423247 1102 16-17 0.46158659066991703 1102 18-19 0.4258097460229422 1102 20-21 0.44423946697335737 1102 22-23 0.4376699084257325 1102 24-25 0.4699263579187445 1102 26-27 0.40464102012184355 1102 28-29 0.3920629005213172 1102 30-31 0.3163559520982915 1102 32-33 0.3850603504807637 1102 34-35 0.33221184780821034 1102 36-37 0.45334474534416813 1102 38-39 0.4569902573472433 1102 40-41 0.4064286558697532 1102 42-43 0.4101446258886554 1102 44-45 0.5328304052731099 1102 46-47 0.523238423025127 1102 48-49 0.4968429382597108 1102 50-51 0.5545771099081307 1102 52-53 0.5155354659876608 1102 54-55 0.3498673672976693 1102 56-57 0.590702968112538 1102 58-59 0.5904747718752148 1102 60-61 0.4788624337799732 1102 62-63 0.6244178253821104 1102 64-65 0.7356133236540074 1102 66-67 0.6290115573318076 1102 68-69 0.7724566817209073 1102 70-71 0.7549393696769933 1102 72-73 0.751840283986148 1102 74-75 0.8089862912655299 1102 76-77 0.7097102281316658 1102 78-79 0.9454839578595333 1102 80-81 0.9745458987027931 1102 82-83 0.8753453305474217 1102 84-85 0.8105172329701738 1102 86-87 0.8599583294747255 1102 88-89 1.0004743273143575 1102 90-91 1.0539199559370012 1102 92-93 1.5667524618861322 1102 94-95 1.6494869524352431 1102 96-97 1.583340564153204 1102 98-99 1.8144934545344675 1102 100-101 2.0357678938829906 1103 1 0.25675811135523574 1103 2 0.24060408051339266 1103 3 0.19327429166465748 1103 4 0.17066355991668303 1103 5 0.15984489143868075 1103 6 0.16721985569559905 1103 7 0.20146363886128427 1103 8 0.18097849065674154 1103 9 0.34240510562518267 1103 10-11 0.31740731199145245 1103 12-13 0.33845338646320755 1103 14-15 0.333900860949079 1103 16-17 0.36053221938870195 1103 18-19 0.4367054449471155 1103 20-21 0.3884421471428965 1103 22-23 0.33961276014719033 1103 24-25 0.4446646290159819 1103 26-27 0.48383148607245374 1103 28-29 0.4367163478923928 1103 30-31 0.31987127877610533 1103 32-33 0.3305197252752947 1103 34-35 0.36302364733492 1103 36-37 0.4585411730404729 1103 38-39 0.44442047707063637 1103 40-41 0.5917320999010443 1103 42-43 0.542399712356108 1103 44-45 0.45133142840033 1103 46-47 0.5411886247293438 1103 48-49 0.7048025070760744 1103 50-51 0.6926402070266491 1103 52-53 0.5778689233205654 1103 54-55 0.5368605016282615 1103 56-57 0.7405701787030452 1103 58-59 0.6273936372669695 1103 60-61 0.725078790177399 1103 62-63 0.7009964523563568 1103 64-65 0.6730696687432385 1103 66-67 0.5133877601581283 1103 68-69 0.585351625137168 1103 70-71 0.5176093000959732 1103 72-73 0.6193412096228563 1103 74-75 0.6411418031160316 1103 76-77 0.6850131681422091 1103 78-79 0.9158066846082633 1103 80-81 0.9197131943489794 1103 82-83 1.035094748525541 1103 84-85 0.9504559761377713 1103 86-87 1.0616286263115526 1103 88-89 1.242644304036972 1103 90-91 1.2108343008382185 1103 92-93 1.527577624066577 1103 94-95 1.5673117331993005 1103 96-97 1.5552118030728117 1103 98-99 1.616619227417143 1103 100-101 1.7094990478236554 1104 1 0.21548229520931628 1104 2 0.24741776200761478 1104 3 0.226065712314373 1104 4 0.2263134198858836 1104 5 0.10996110849592355 1104 6 0.11440438116679275 1104 7 0.19510421947629908 1104 8 0.20899440583980322 1104 9 0.28743213622183816 1104 10-11 0.2684219224216662 1104 12-13 0.29418171670975823 1104 14-15 0.38471693182196987 1104 16-17 0.4000994246661449 1104 18-19 0.3348230337958782 1104 20-21 0.3972240893235437 1104 22-23 0.37626065598308855 1104 24-25 0.34755959941389847 1104 26-27 0.29709636490754576 1104 28-29 0.32375817102433757 1104 30-31 0.33688647154232854 1104 32-33 0.4615743437901827 1104 34-35 0.5183681561164875 1104 36-37 0.40795836175836087 1104 38-39 0.3223908159561688 1104 40-41 0.37333671076151376 1104 42-43 0.49825961852197054 1104 44-45 0.5186362632900696 1104 46-47 0.5264896434171291 1104 48-49 0.6104745798620641 1104 50-51 0.6623916285968932 1104 52-53 0.7150368191964844 1104 54-55 0.6560935490966457 1104 56-57 0.5169111274304612 1104 58-59 0.6754522420819526 1104 60-61 0.7934881373707299 1104 62-63 0.6665969788601487 1104 64-65 0.7617021847049443 1104 66-67 0.7715988729702197 1104 68-69 0.9564832680951518 1104 70-71 0.9386821341212048 1104 72-73 0.8253411966153514 1104 74-75 1.0416822899769507 1104 76-77 0.9574423597857873 1104 78-79 1.1525560389857397 1104 80-81 1.1753115495999573 1104 82-83 1.3306076344607263 1104 84-85 1.3152215708859423 1104 86-87 1.3577741272655999 1104 88-89 1.3334793408532128 1104 90-91 1.4667741031041288 1104 92-93 1.7462072733742282 1104 94-95 1.7786884475634892 1104 96-97 1.66802126913473 1104 98-99 1.6717631704722855 1104 100-101 1.7367118746247208 1105 1 0.20694017606566106 1105 2 0.24199288611558956 1105 3 0.17002514452038753 1105 4 0.11241268040920716 1105 5 0.09005955456066772 1105 6 0.17433237268608082 1105 7 0.22460701190571797 1105 8 0.19163577257886288 1105 9 0.2633383829898577 1105 10-11 0.1863768557136325 1105 12-13 0.29249058396593597 1105 14-15 0.36482200344261884 1105 16-17 0.2736109369735189 1105 18-19 0.28173568634526447 1105 20-21 0.28103042270858225 1105 22-23 0.2755599630469021 1105 24-25 0.2828466637432001 1105 26-27 0.19017993094332297 1105 28-29 0.2362589790189631 1105 30-31 0.30960469472984187 1105 32-33 0.32812902240223707 1105 34-35 0.3943503543789788 1105 36-37 0.4125345200994488 1105 38-39 0.22625723182130741 1105 40-41 0.28799502599576243 1105 42-43 0.39953059406236235 1105 44-45 0.389524540257689 1105 46-47 0.3652638509360031 1105 48-49 0.5835156777005182 1105 50-51 0.6832555041269401 1105 52-53 0.6337165236643685 1105 54-55 0.7181344465524333 1105 56-57 0.6510991574749383 1105 58-59 0.6380163968549368 1105 60-61 0.6741479780988371 1105 62-63 0.6668611931015391 1105 64-65 0.5746981318039204 1105 66-67 0.6671170884009747 1105 68-69 0.6302284524472057 1105 70-71 0.7184064786759663 1105 72-73 0.7439942372607149 1105 74-75 0.7901941254821736 1105 76-77 0.6687681820011129 1105 78-79 0.8576174788967954 1105 80-81 0.8505696230822188 1105 82-83 0.8393210484642424 1105 84-85 0.9642498765535379 1105 86-87 0.9594613816352364 1105 88-89 1.1782868442144725 1105 90-91 1.3424235691812818 1105 92-93 1.546951274822156 1105 94-95 1.662599497051545 1105 96-97 1.7256488999652184 1105 98-99 1.8000515469848501 1105 100-101 1.5302299801682118 1106 1 -0.027847474458912558 1106 2 0.0622137308620907 1106 3 0.04311193195126606 1106 4 0.07453249280261076 1106 5 -0.007300612631432557 1106 6 -0.009110893115767738 1106 7 0.014156213414835861 1106 8 0.010998045822958602 1106 9 -0.2529047045082038 1106 10-11 0.048936613323846245 1106 12-13 0.05570064973160527 1106 14-15 0.007012249062547937 1106 16-17 0.06635305219911203 1106 18-19 -4.338540702590876E-4 1106 20-21 0.1855979229669913 1106 22-23 0.2536815713987579 1106 24-25 0.12814664694646893 1106 26-27 0.0934514178418766 1106 28-29 0.1626585184008391 1106 30-31 0.15062554852073617 1106 32-33 0.1362151153527762 1106 34-35 0.24107386076173754 1106 36-37 0.28851664449864245 1106 38-39 0.12648544128950334 1106 40-41 0.2406046569844449 1106 42-43 0.1660358882636075 1106 44-45 0.09943881231309604 1106 46-47 -0.514729058904301 1106 48-49 -0.22535477426082196 1106 50-51 0.0758994230633121 1106 52-53 0.024907848151421774 1106 54-55 0.262387460274077 1106 56-57 0.23931616804394906 1106 58-59 0.03890105174666303 1106 60-61 0.18155275353942812 1106 62-63 0.0072635556267357515 1106 64-65 0.14313278927976114 1106 66-67 0.012605711844049239 1106 68-69 -0.11854756633929497 1106 70-71 -0.05966498173542334 1106 72-73 0.3004916660684067 1106 74-75 0.40961316845597295 1106 76-77 0.23456954770476912 1106 78-79 0.45532981222151747 1106 80-81 -0.07811249550271882 1106 82-83 0.5404102520406546 1106 84-85 0.5621771757679674 1106 86-87 0.7641043056881571 1106 88-89 0.8627889502664665 1106 90-91 0.7306010105219194 1106 92-93 0.7563861939394201 1106 94-95 0.980772997620047 1106 96-97 1.0503193448202524 1106 98-99 0.9285248369481565 1106 100-101 1.030228607358243 1107 1 0.11292379187070622 1107 2 0.18505483140967982 1107 3 0.16905331229855847 1107 4 0.14768915586425635 1107 5 0.12293120752161002 1107 6 0.08628236767179231 1107 7 0.13177556728855677 1107 8 0.14327027465979114 1107 9 0.16504512219659517 1107 10-11 0.15694578967542583 1107 12-13 0.17829598874455854 1107 14-15 0.3322957881038988 1107 16-17 0.31085904721270197 1107 18-19 0.27460371961507946 1107 20-21 0.2600076170875667 1107 22-23 0.1898770166496675 1107 24-25 0.15183092513817797 1107 26-27 0.1953973004464089 1107 28-29 0.23352877606554756 1107 30-31 0.14269838675210167 1107 32-33 0.2039193216300319 1107 34-35 0.3005873485623951 1107 36-37 0.248615319126678 1107 38-39 0.2804076542753009 1107 40-41 0.27492935132294605 1107 42-43 0.24799222533648901 1107 44-45 0.2622891067722577 1107 46-47 0.22411603277925707 1107 48-49 0.3272087968400683 1107 50-51 0.3407212345837394 1107 52-53 0.4509566737170232 1107 54-55 0.40866080605201205 1107 56-57 0.3105270425724669 1107 58-59 0.5137617241627623 1107 60-61 0.45223678118763644 1107 62-63 0.7365296701360862 1107 64-65 0.4438609489578056 1107 66-67 0.5739742876688929 1107 68-69 0.6998047918896155 1107 70-71 0.9320126782196674 1107 72-73 0.891768205570397 1107 74-75 0.8184185494655232 1107 76-77 0.8432227211877965 1107 78-79 1.1339949202295934 1107 80-81 1.2409435168043323 1107 82-83 1.3102569658287315 1107 84-85 1.3953208507048664 1107 86-87 1.4614217342652296 1107 88-89 1.4239692265039956 1107 90-91 1.6613048025714434 1107 92-93 1.7955782993420328 1107 94-95 1.9412200500024532 1107 96-97 1.8498620480622954 1107 98-99 1.8632454778127077 1107 100-101 1.9889326734691188 1108 1 0.11715140944306413 1108 2 0.12009099185217309 1108 3 0.19351404334611289 1108 4 0.14272170521673644 1108 5 0.05916849576916405 1108 6 0.15942015167356516 1108 7 0.20539446297759412 1108 8 0.17505667428194727 1108 9 0.23783776085749508 1108 10-11 0.22135090112929134 1108 12-13 0.22236642481669833 1108 14-15 0.3748631630066015 1108 16-17 0.3993263486825711 1108 18-19 0.3765115971859174 1108 20-21 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-1.6424391358155859 2316 90-91 -1.5697091981459543 2316 92-93 -1.8565665491163088 2316 94-95 -1.656661412867198 2316 96-97 -1.6707983716794992 2316 98-99 -1.577993097236856 2316 100-101 -1.924836334243773 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 17.0 4 39.0 5 114.0 6 248.0 7 633.0 8 1509.0 9 2862.0 10 4470.0 11 7068.0 12 10091.0 13 14077.0 14 18388.0 15 24000.0 16 29486.0 17 36173.0 18 34688.0 19 32013.0 20 32942.0 21 34511.0 22 37631.0 23 42431.0 24 49261.0 25 56998.0 26 67019.0 27 80050.0 28 94763.0 29 112065.0 30 132764.0 31 156316.0 32 187068.0 33 229930.0 34 289299.0 35 373494.0 36 477217.0 37 502857.0 38 215630.0 39 7103.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.865495450068565 19.229144773116055 15.298997177204631 23.606362599610748 2 16.05380481403739 20.249445181321317 39.262332062552254 24.43441794208904 3 19.53802259698972 23.26262925850995 29.88117745154895 27.318170692951377 4 13.54564682049616 16.88220919283548 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26.087047448012314 28.78973422909885 22.47654315060292 26-27 22.20580341265464 26.46145281919911 28.592458523647284 22.740285244498967 28-29 22.33884336832017 26.853413001978215 28.41709227608914 22.390651353612473 30-31 22.451377654596342 26.362813671470303 28.334657517401546 22.85115115653181 32-33 22.105564510974716 26.321559207036493 28.448509710583696 23.12436657140509 34-35 22.93442413373803 26.470727721325694 28.182366879383153 22.412481265553126 36-37 23.058905948425053 26.073751343933772 28.147802591871745 22.719540115769426 38-39 22.49492351547805 26.393021520052923 28.174386006045243 22.93766895842378 40-41 23.052597893457012 26.1564212950493 28.195530521398915 22.595450290094774 42-43 22.671107477197058 26.33097665629116 27.956901203354406 23.041014663157373 44-45 22.61852110241831 26.4600011576002 28.28977531215809 22.63170242782341 46-47 23.099694836669435 26.758974573453177 27.45514222743607 22.686188362441317 48-49 22.79793333649463 26.804921373538352 27.416337798517983 22.980807491449035 50-51 22.99651685962467 26.923782668063147 27.452144372722525 22.627556099589658 52-53 23.343345415967278 26.575673348882596 27.440621860486075 22.64035937466405 54-55 22.71735155802789 26.777659521670984 27.675949402880295 22.82903951742083 56-57 22.844410291965406 26.916165645283048 27.44414150318491 22.79528255956664 58-59 22.883030156087646 26.59029960473586 27.39290645670757 23.133763782468915 60-61 22.416236185402408 26.850352744776423 27.726316707169556 23.007094362651614 62-63 22.64656166929879 26.82687805761006 27.75274929928197 22.773810973809177 64-65 22.3166111880284 26.905440834241613 27.59295372197838 23.18499425575161 66-67 22.207628943351697 26.921860208104935 27.565995231836766 23.3045156167066 68-69 22.830706417275344 26.46857642801278 27.676250822982535 23.024466331729347 70-71 22.614621235817587 26.54145850673858 27.322511078791223 23.521409178652615 72-73 22.59188959096874 26.69193643610837 27.377751636524128 23.33842233639876 74-75 22.607173732089716 26.974725091977252 27.175022429972895 23.243078745960137 76-77 22.65012932688872 26.667167619582248 27.413115978703605 23.26958707482542 78-79 22.711117468371363 26.892772349964034 27.376745793442304 23.019364388222304 80-81 22.90382472093764 26.835621818023263 27.16122526119424 23.099328199844855 82-83 22.80961373066834 26.93681958550716 27.22608183492002 23.027484848904486 84-85 22.710746908110554 26.996207015195505 27.19850837543833 23.094537701255607 86-87 22.933665872433533 27.074360899575804 26.847240071766702 23.14473315622396 88-89 22.6421938244154 27.221676715229727 26.666428877368226 23.469700582986647 90-91 22.607158318722078 27.13976735221103 26.837751522121188 23.415322806945703 92-93 22.71403229182517 26.81366614340109 27.07584886055323 23.39645270422051 94-95 22.474902406225496 26.658313445822916 27.207101200115076 23.659682947836515 96-97 22.592222812985657 26.30234977998113 27.262612909845817 23.84281449718739 98-99 22.375366447927558 26.613962211217817 26.86234229625377 24.148329044600853 100-101 22.46800835465668 26.283410562799332 27.33070309817782 23.91787798436617 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 315.0 1 218.5 2 177.5 3 243.5 4 216.0 5 215.0 6 416.0 7 941.5 8 1532.0 9 1762.0 10 1563.0 11 1351.5 12 1414.0 13 1580.5 14 1820.0 15 2097.5 16 2356.5 17 2702.5 18 3105.0 19 3596.5 20 4236.5 21 5061.5 22 6137.5 23 7780.0 24 10155.5 25 13173.0 26 17236.5 27 21906.0 28 26397.5 29 31315.5 30 37227.5 31 45655.0 32 57708.0 33 62677.0 34 62076.5 35 65899.0 36 72190.0 37 78619.5 38 84211.5 39 88986.5 40 95149.5 41 102800.0 42 107336.5 43 110516.0 44 114518.5 45 121245.0 46 125485.0 47 129713.5 48 132845.5 49 130421.5 50 127367.0 51 122296.0 52 118604.5 53 119793.0 54 122647.5 55 116983.0 56 107103.5 57 97981.0 58 90603.5 59 80493.5 60 64201.0 61 52845.5 62 45317.0 63 34553.0 64 24294.5 65 18179.0 66 13694.0 67 10689.5 68 8265.0 69 6273.0 70 4787.0 71 3261.0 72 2283.5 73 1869.0 74 1453.0 75 1064.5 76 774.0 77 494.5 78 342.0 79 233.0 80 135.5 81 83.5 82 46.5 83 29.5 84 19.5 85 12.0 86 5.0 87 3.0 88 1.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.4179615466517595E-4 2 0.05242647702026755 3 0.0 4 0.0 5 0.04647695547077651 6 5.890615395535679E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006494403473578087 26-27 0.002164801157859362 28-29 4.417961546651759E-5 30-31 0.0017230050031941863 32-33 5.301553855982112E-4 34-35 0.012223026945736536 36-37 0.023901171967386017 38-39 0.004859757701316936 40-41 0.0013990211564397239 42-43 0.0016199192337723119 44-45 0.008143775784328077 46-47 0.0 48-49 0.0 50-51 5.596084625758896E-4 52-53 0.0 54-55 0.001693551926216508 56-57 0.0 58-59 0.02332683696632129 60-61 0.0 62-63 0.009439711171345925 64-65 0.015639583875147228 66-67 0.006273505396245499 68-69 0.016670441569365972 70-71 1.4726538488839198E-4 72-73 4.417961546651759E-5 74-75 0.022899767350144953 76-77 0.05002605124658676 78-79 0.03052811428736366 80-81 0.02703792466550877 82-83 0.03687525237605335 84-85 0.01599302079887937 86-87 0.010072952326366012 88-89 0.08458923707989235 90-91 0.0256978096630244 92-93 0.02481421735369405 94-95 0.027435541204707425 96-97 0.038804428918091285 98-99 0.05428202086986128 100-101 0.014063844256841434 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3395231.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.86670859166974 #Duplication Level Percentage of deduplicated Percentage of total 1 73.01417478834951 37.139907520202144 2 14.640285425666546 14.894062648924997 3 5.256941237594632 8.022098940487735 4 2.396892421756738 4.876881133723262 5 1.3000423432476367 3.3064437515404514 6 0.779026475886039 2.377590764045435 7 0.5154380725750228 1.8353046764307923 8 0.35166021872964237 1.4310238295522848 9 0.2607587722098406 1.1937546430847596 >10 1.341030514417984 12.2873823767378 >50 0.09237366221379613 3.234588734952495 >100 0.04435928729425575 4.255760149148802 >500 0.0037113547456634212 1.264000052260373 >1k 0.0031894454772436937 3.4577042743313005 >5k 1.1597983553613432E-4 0.4234965045774993 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7833 0.23070595196615487 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6524 0.19215187420237387 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4655 0.13710407333109292 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4646 0.13683899563829383 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4507 0.13274501793839655 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4287 0.1262653410033073 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4238 0.12482214023140105 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4186 0.12329058022856178 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3849 0.11336489328708414 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3407 0.10034663326295029 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.9453076977678396E-5 2 0.0 0.0 0.0 2.9453076977678396E-5 2.9453076977678396E-5 3 0.0 0.0 0.0 2.9453076977678396E-5 2.9453076977678396E-5 4 0.0 0.0 0.0 2.9453076977678396E-5 2.9453076977678396E-5 5 0.0 0.0 0.0 2.9453076977678396E-5 2.9453076977678396E-5 6 0.0 0.0 0.0 2.9453076977678396E-5 2.9453076977678396E-5 7 2.9453076977678396E-5 0.0 0.0 2.9453076977678396E-5 2.9453076977678396E-5 8 2.9453076977678396E-5 0.0 0.0 2.9453076977678396E-5 2.9453076977678396E-5 9 2.9453076977678396E-5 0.0 0.0 2.9453076977678396E-5 2.9453076977678396E-5 10-11 8.835923093303519E-5 0.0 0.0 2.9453076977678396E-5 2.9453076977678396E-5 12-13 8.835923093303519E-5 0.0 0.0 2.9453076977678396E-5 3.2398384675446236E-4 14-15 8.835923093303519E-5 0.0 0.0 7.363269244419599E-5 4.2706961617633676E-4 16-17 1.0308576942187438E-4 0.0 0.0 8.835923093303519E-5 4.5652269315401515E-4 18-19 1.325388463995528E-4 0.0 0.0 8.835923093303519E-5 4.7124923164285434E-4 20-21 1.7671846186607037E-4 0.0 0.0 1.1781230791071358E-4 5.448819240870504E-4 22-23 1.9144500035490956E-4 0.0 0.0 2.2089807733258798E-4 5.596084625758896E-4 24-25 2.503511543102664E-4 0.0 0.0 4.2706961617633676E-4 5.596084625758896E-4 26-27 2.650776927991056E-4 0.0 0.0 6.921473089754424E-4 6.479676935089247E-4 28-29 2.798042312879448E-4 0.0 0.0 0.0022531603887923973 6.774207704866031E-4 30-31 3.2398384675446236E-4 0.0 0.0 0.008953735401214233 6.774207704866031E-4 32-33 3.2398384675446236E-4 0.0 0.0 0.023591914659120396 6.774207704866031E-4 34-35 3.8289000070981913E-4 0.0 0.0 0.04525465277620286 7.068738474642815E-4 36-37 4.4179615466517595E-4 0.0 0.0 0.08146721092025845 7.068738474642815E-4 38-39 4.4179615466517595E-4 0.0 0.0 0.136824269099805 8.099596168861559E-4 40-41 4.4179615466517595E-4 0.0 0.0 0.19646674997960373 8.246861553749951E-4 42-43 4.5652269315401515E-4 0.0 0.0 0.2610131681761859 8.246861553749951E-4 44-45 5.301553855982112E-4 0.0 0.0 0.34287799563564303 8.688657708415127E-4 46-47 5.743350010647287E-4 0.0 0.0 0.44035295389326967 9.277719247968695E-4 48-49 5.890615395535679E-4 0.0 0.0 0.5417304448504388 9.424984632857087E-4 50-51 5.890615395535679E-4 0.0 0.0 0.6554340485227662 9.719515402633871E-4 52-53 6.037880780424071E-4 0.0 0.0 0.7656474625732388 0.0011339434636406182 54-55 6.479676935089247E-4 0.0 0.0 0.8860810943349657 0.0012517557715513317 56-57 6.774207704866031E-4 0.0 0.0 1.0294292199853265 0.0013106619255066885 58-59 6.774207704866031E-4 0.0 0.0 1.1807149498811715 0.0013548415409732062 60-61 6.774207704866031E-4 0.0 0.0 1.3478906148064742 0.0013548415409732062 62-63 6.774207704866031E-4 0.0 0.0 1.5163033089648392 0.001413747694928563 64-65 6.774207704866031E-4 0.0 0.0 1.693625558908952 0.001413747694928563 66-67 7.068738474642815E-4 0.0 0.0 1.9036259977597991 0.001428474233417402 68-69 7.363269244419599E-4 0.0 0.0 2.1359076893442595 0.0014432007719062414 70-71 7.510534629307992E-4 0.0 0.0 2.38265084172476 0.0014873803873727588 72-73 7.952330783973167E-4 0.0 0.0 2.6382888233525197 0.0015610130798169551 74-75 8.246861553749951E-4 0.0 0.0 2.8982122276805318 0.0016051926952834728 76-77 8.541392323526735E-4 0.0 0.0 3.1798572762795816 0.0016199192337723119 78-79 8.835923093303519E-4 0.0 0.0 3.506477173423546 0.001634645772261151 80-81 9.424984632857087E-4 0.0 0.0 3.8444217786654282 0.0016788253877276687 82-83 9.866780787522263E-4 0.0 0.0 4.193160936619629 0.0017671846186607038 84-85 0.0010161311557299046 1.4726538488839198E-5 0.0 4.555860853061249 0.0017671846186607038 86-87 0.001030857694218744 2.9453076977678396E-5 0.0 4.939619719541911 0.0018849969265714176 88-89 0.001030857694218744 2.9453076977678396E-5 0.0 5.344393356446145 0.002002809234482131 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5950 0.0 42.656746 1 GTATCAA 9595 0.0 37.003178 1 ATCAACG 12160 0.0 28.985296 3 TATCAAC 12345 0.0 28.605843 2 TCAACGC 12415 0.0 28.389948 4 CAACGCA 12640 0.0 27.884588 5 AACGCAG 13145 0.0 26.80306 6 GTAAGGT 2390 0.0 25.241388 4 TAAGGTG 2530 0.0 24.220139 5 GGTAAGG 2510 0.0 23.46688 3 AGGTAAG 2425 0.0 23.323418 2 ACGCAGA 15025 0.0 23.322924 7 CGCAGAG 15095 0.0 23.151855 8 AAGGTAA 2445 0.0 22.355398 1 TACATGG 9140 0.0 21.52837 2 GTACATG 9525 0.0 21.207397 1 ACATGGG 9070 0.0 20.948847 3 AGAGTAC 13880 0.0 20.645666 10-11 GAGTACT 9995 0.0 20.309275 12-13 GCAGAGT 16795 0.0 20.242966 9 >>END_MODULE