FastQCFastQC Report
Thu 26 May 2016
SRR938386_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938386_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3385833
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78030.2304602737347058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59410.1754664214094434No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA48050.14191485522174307No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA47060.13899090711207554No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA43230.1276790674554829No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA42970.12691116189132776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41430.12236279816517825No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA37240.10998770465052471No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC36320.10727050034659122No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG34990.1033423680376439No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53350.045.497281
GTATCAA93200.039.0656661
ATCAACG117250.030.5608713
TATCAAC119200.030.1805292
TCAACGC120000.029.7813134
CAACGCA121550.029.2833825
AACGCAG127650.027.9539346
ACGCAGA144300.024.4954517
CGCAGAG145500.024.0648618
GTACATG99200.022.069671
TACATGG95550.021.5857092
GCAGAGT161400.021.3096039
TAAGGTG23650.021.2994865
AGAGTAC135450.020.90202510-11
GTGTAAG14350.020.8948061
AGGACCG11900.019.967215
GAGTACT97400.019.92150512-13
GTAAGGT23150.019.9126014
ACATGGG96900.019.8136653
GTATAGC18700.019.3429031
Produced by FastQC (version 0.11.5)