##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938386_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3385833 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.97496598326025 31.0 31.0 34.0 28.0 34.0 2 31.08733833003577 31.0 31.0 34.0 27.0 34.0 3 31.156907916013576 31.0 31.0 34.0 27.0 34.0 4 34.78165225514667 37.0 35.0 37.0 32.0 37.0 5 34.79410738804897 37.0 35.0 37.0 32.0 37.0 6 34.821929197334896 37.0 35.0 37.0 32.0 37.0 7 34.73448690469967 37.0 35.0 37.0 31.0 37.0 8 34.69089556395723 37.0 35.0 37.0 31.0 37.0 9 36.186139127358025 39.0 35.0 39.0 32.0 39.0 10-11 36.03163431273781 39.0 35.0 39.0 30.0 39.0 12-13 35.877629079756744 38.0 35.0 39.0 30.0 39.0 14-15 36.858224106150544 39.0 36.0 41.0 30.5 41.0 16-17 36.695071050462325 39.0 36.0 41.0 30.0 41.0 18-19 36.673586381844586 39.0 36.0 40.5 30.0 41.0 20-21 36.75486283582209 39.0 36.0 41.0 30.0 41.0 22-23 36.67377126987657 39.0 36.0 41.0 30.0 41.0 24-25 36.54662707818135 39.0 36.0 41.0 30.0 41.0 26-27 36.35079270005343 39.0 35.5 41.0 29.0 41.0 28-29 36.154289653388105 39.0 35.0 40.5 28.5 41.0 30-31 35.92500353679582 39.0 35.0 40.0 27.0 41.0 32-33 35.631434716360786 38.0 34.5 40.0 27.0 41.0 34-35 35.36873643797553 38.0 34.0 40.0 26.0 41.0 36-37 35.060839828780686 38.0 34.0 40.0 25.0 41.0 38-39 34.7754494979522 38.0 33.5 40.0 24.5 41.0 40-41 34.68787680904522 38.0 33.5 40.0 24.0 41.0 42-43 34.48796056982137 38.0 33.0 40.0 24.0 41.0 44-45 34.19238160889802 38.0 33.0 40.0 23.5 41.0 46-47 33.82659821083911 38.0 33.0 40.0 21.5 41.0 48-49 33.45136381504935 38.0 32.5 40.0 19.5 41.0 50-51 32.70742620796713 36.5 31.5 39.5 18.0 40.5 52-53 32.75971511294266 37.0 31.5 39.0 18.0 40.0 54-55 32.86745802288536 37.0 31.5 40.0 16.0 41.0 56-57 32.539828308129785 37.0 31.0 40.0 13.0 41.0 58-59 32.18307636555022 36.0 31.0 39.0 8.5 41.0 60-61 31.70812352528905 36.0 30.5 39.0 7.5 40.5 62-63 31.157752464460003 35.0 29.5 39.0 4.5 40.0 64-65 30.641431665412917 35.0 29.0 38.5 2.0 40.0 66-67 30.075794346620167 34.5 28.0 38.0 2.0 40.0 68-69 29.490450946635583 34.0 28.0 37.5 2.0 40.0 70-71 28.83290685039693 34.0 26.0 37.0 2.0 39.0 72-73 28.25261479228302 34.0 26.0 36.0 2.0 39.0 74-75 27.610267842507294 33.0 25.5 36.0 2.0 38.5 76-77 26.84818004904554 32.5 23.5 35.0 2.0 37.5 78-79 26.0374166416359 32.0 21.0 35.0 2.0 37.0 80-81 25.351822136531837 31.0 19.0 35.0 2.0 36.0 82-83 24.65323201114763 31.0 16.5 34.0 2.0 36.0 84-85 23.96746694240383 31.0 9.0 34.0 2.0 35.0 86-87 23.27115513375881 30.0 4.5 34.0 2.0 35.0 88-89 22.442821456344717 29.5 2.0 34.0 2.0 35.0 90-91 21.579943251778808 29.0 2.0 34.0 2.0 35.0 92-93 20.689840727525546 28.0 2.0 34.0 2.0 35.0 94-95 19.695345281353216 26.5 2.0 33.5 2.0 35.0 96-97 18.442414466395714 25.0 2.0 33.0 2.0 34.5 98-99 16.952333000475804 19.0 2.0 32.0 2.0 34.0 100-101 13.636492260545632 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6353877424747765 1101 2 0.6230553091370226 1101 3 0.6334344860092074 1101 4 0.5964604086908167 1101 5 0.4593738148058861 1101 6 0.6328748063918397 1101 7 0.5542584850579004 1101 8 0.5736851939083039 1101 9 0.5932924445249412 1101 10-11 0.6619219405818555 1101 12-13 0.5979745464160757 1101 14-15 0.8012874328683068 1101 16-17 0.8169918257726039 1101 18-19 0.758798470789543 1101 20-21 0.8580430085200916 1101 22-23 0.9646991344087112 1101 24-25 0.914485171159221 1101 26-27 0.8395539171244906 1101 28-29 0.9711020672346962 1101 30-31 0.8730863970740117 1101 32-33 0.8981804798362205 1101 34-35 0.8062808034941256 1101 36-37 0.7912504602006152 1101 38-39 0.9054126488898717 1101 40-41 1.016087529211724 1101 42-43 0.9810619012054005 1101 44-45 0.8498376538255954 1101 46-47 0.8707856145217718 1101 48-49 0.9064597998812189 1101 50-51 0.7451686648486913 1101 52-53 0.7976574688565847 1101 54-55 0.9278250779985413 1101 56-57 0.8971801546236335 1101 58-59 0.8936937428400498 1101 60-61 0.8308794466462182 1101 62-63 0.7250073803309327 1101 64-65 0.8079231624707823 1101 66-67 0.7151792084430788 1101 68-69 0.8345260936428787 1101 70-71 0.9577758369741183 1101 72-73 0.9364294991858095 1101 74-75 0.9228387209241333 1101 76-77 0.975212671395937 1101 78-79 0.9674783885385345 1101 80-81 1.1706684188224266 1101 82-83 1.323648030543275 1101 84-85 1.257790289292128 1101 86-87 1.4827080038369225 1101 88-89 1.5061600880634138 1101 90-91 1.4829479026421062 1101 92-93 1.4277622801409713 1101 94-95 1.366665662105838 1101 96-97 1.47114510854448 1101 98-99 1.4878206853947695 1101 100-101 1.0616344656579848 1102 1 0.6702958414138074 1102 2 0.6079336437201697 1102 3 0.5929376235479857 1102 4 0.6382977508205343 1102 5 0.6516186829830275 1102 6 0.6222705321725357 1102 7 0.5094190608681473 1102 8 0.6275236399199571 1102 9 0.7259443041469922 1102 10-11 0.8025010884832469 1102 12-13 0.8382769291538992 1102 14-15 0.9062665689217511 1102 16-17 0.9964819088960724 1102 18-19 1.050317399330531 1102 20-21 0.8231256985243292 1102 22-23 0.9413209305864427 1102 24-25 0.8711505807875497 1102 26-27 0.9221662595070512 1102 28-29 0.7687024605287505 1102 30-31 0.868143491177058 1102 32-33 0.8538906486983606 1102 34-35 0.8878010035221493 1102 36-37 1.0139035551879232 1102 38-39 1.1452414749461042 1102 40-41 1.0760590941377544 1102 42-43 1.1635681538874465 1102 44-45 1.1014759576618758 1102 46-47 1.138897080589679 1102 48-49 1.0184109834478292 1102 50-51 1.104080380301447 1102 52-53 1.132606349297788 1102 54-55 1.0171470430817777 1102 56-57 1.2297737136748097 1102 58-59 1.4670859980390532 1102 60-61 1.2275806963156946 1102 62-63 1.1964075909932212 1102 64-65 1.3771568964670173 1102 66-67 1.3503595934435815 1102 68-69 1.1940377920461493 1102 70-71 1.1423811269381865 1102 72-73 1.350040984759488 1102 74-75 1.180233574002358 1102 76-77 1.1690956628003946 1102 78-79 1.2758739597179698 1102 80-81 1.3267127503742309 1102 82-83 1.363473027689654 1102 84-85 1.1384617717159848 1102 86-87 1.266344435119553 1102 88-89 1.4159966658560244 1102 90-91 1.5435707434620447 1102 92-93 1.5930953302794642 1102 94-95 1.4910833788329683 1102 96-97 1.4985973085782867 1102 98-99 1.5892893251781537 1102 100-101 1.154117629291143 1103 1 0.5352259072433618 1103 2 0.5625599782397437 1103 3 0.4764627478540646 1103 4 0.5247490366399319 1103 5 0.586091138457526 1103 6 0.563712534603134 1103 7 0.39829004719058503 1103 8 0.44042599361447543 1103 9 0.5096403071525444 1103 10-11 0.5067440745690632 1103 12-13 0.49171658297535004 1103 14-15 0.7085755329702863 1103 16-17 0.7478535308893512 1103 18-19 0.75017140260384 1103 20-21 0.6496666943636669 1103 22-23 0.7500691104921842 1103 24-25 0.8276058311173244 1103 26-27 0.7916618689674948 1103 28-29 0.6586906707806506 1103 30-31 0.808172877331792 1103 32-33 1.0460848183773308 1103 34-35 1.1508783093986565 1103 36-37 0.9117650028153861 1103 38-39 0.8166109382531133 1103 40-41 0.9033475869907406 1103 42-43 0.7229695180111122 1103 44-45 0.9507865531790785 1103 46-47 0.8879965036600126 1103 48-49 0.8284931640115545 1103 50-51 0.9140914695143465 1103 52-53 0.8913383909479791 1103 54-55 0.9090251416527835 1103 56-57 0.9479612422800017 1103 58-59 0.8594152931760988 1103 60-61 0.8425599293386163 1103 62-63 1.0446653080371853 1103 64-65 0.9473874970903431 1103 66-67 1.115216647936915 1103 68-69 1.067449652781825 1103 70-71 1.1124837112385144 1103 72-73 1.1129658593636869 1103 74-75 1.0899880338170647 1103 76-77 0.9197139499866189 1103 78-79 0.9962203043252522 1103 80-81 1.0094576081863416 1103 82-83 1.2190490025492586 1103 84-85 1.4062765118070466 1103 86-87 1.49929678108224 1103 88-89 1.4272078237732693 1103 90-91 1.2715787378660615 1103 92-93 1.384196509358059 1103 94-95 1.095808462871247 1103 96-97 1.340794617692822 1103 98-99 1.5383889558144794 1103 100-101 0.9987822493571699 1104 1 0.541361232232088 1104 2 0.538359459657201 1104 3 0.5519218763964453 1104 4 0.4633317642167327 1104 5 0.4356502777627469 1104 6 0.5079511743208514 1104 7 0.4693270837235062 1104 8 0.3803415998133701 1104 9 0.5019331532894995 1104 10-11 0.5119132642025548 1104 12-13 0.4671078577119303 1104 14-15 0.6724097317421212 1104 16-17 0.6960824885848211 1104 18-19 0.7292872009711644 1104 20-21 0.6682165940070846 1104 22-23 0.7213027012515667 1104 24-25 0.7983762667201049 1104 26-27 0.7623308515359781 1104 28-29 0.780672253967218 1104 30-31 0.7472456041599784 1104 32-33 0.6644808874944488 1104 34-35 0.8224708044451106 1104 36-37 0.7994925380259019 1104 38-39 0.909084055719596 1104 40-41 0.7884805711239906 1104 42-43 0.7066268835140121 1104 44-45 0.7781873415636724 1104 46-47 0.6969243958843734 1104 48-49 0.7447445033806517 1104 50-51 0.6086357916354146 1104 52-53 0.8421370720837373 1104 54-55 0.9646405608000066 1104 56-57 0.9201776041716627 1104 58-59 0.953875881886006 1104 60-61 1.0821759907276594 1104 62-63 1.0025047037249415 1104 64-65 1.0518461878093142 1104 66-67 1.1334333279248057 1104 68-69 0.9570465704243958 1104 70-71 1.1277849712978032 1104 72-73 1.1254551689864982 1104 74-75 1.1127660674329292 1104 76-77 1.0738207310066095 1104 78-79 1.0124390526947558 1104 80-81 1.0033926071642476 1104 82-83 1.1464630743497786 1104 84-85 1.0283935757537144 1104 86-87 1.1109014202459306 1104 88-89 1.1702328331434941 1104 90-91 1.2071319896185564 1104 92-93 1.1444704249889561 1104 94-95 1.0614085745830124 1104 96-97 0.8271545407952026 1104 98-99 0.9460311545298445 1104 100-101 0.7918636817379046 1105 1 0.4230090903435766 1105 2 0.3781123727395048 1105 3 0.37732979807876177 1105 4 0.41123942259157076 1105 5 0.33825872253559197 1105 6 0.3088381824225266 1105 7 0.3387830474432221 1105 8 0.36520041577803397 1105 9 0.5452250008391886 1105 10-11 0.4278782215631338 1105 12-13 0.5941086974271386 1105 14-15 0.6622861022216782 1105 16-17 0.5601480052227856 1105 18-19 0.5604534625336441 1105 20-21 0.559889930446829 1105 22-23 0.6518983744847375 1105 24-25 0.6952600995590004 1105 26-27 0.6495804174372282 1105 28-29 0.5850486906322416 1105 30-31 0.6795936142726262 1105 32-33 0.622335831097466 1105 34-35 0.6126502346600162 1105 36-37 0.7331427419303864 1105 38-39 0.6839602602232375 1105 40-41 0.6720159237900347 1105 42-43 0.7743454276961899 1105 44-45 0.6009938263453236 1105 46-47 0.7166074187607805 1105 48-49 0.7572239254402078 1105 50-51 0.5841358197078819 1105 52-53 0.5515301616660722 1105 54-55 0.5477065499570699 1105 56-57 0.8360355992727122 1105 58-59 0.9150682938767574 1105 60-61 0.9151863062335401 1105 62-63 0.9043740843642816 1105 64-65 0.9214750533577565 1105 66-67 0.9301647497892844 1105 68-69 0.7943142297500074 1105 70-71 0.7553850045749506 1105 72-73 0.7224770282200694 1105 74-75 0.7832101411295156 1105 76-77 0.6509325286979433 1105 78-79 0.7088762258912418 1105 80-81 0.6951282767828282 1105 82-83 0.817570068165228 1105 84-85 0.6625772385594964 1105 86-87 0.7841078250312847 1105 88-89 0.7988289268489872 1105 90-91 0.8534608686499787 1105 92-93 0.8856415100985409 1105 94-95 0.7759034770731859 1105 96-97 0.9152398876623344 1105 98-99 0.9566754976401839 1105 100-101 0.7856180283603749 1106 1 0.3016277616161993 1106 2 0.35654810766727607 1106 3 0.21995094336323007 1106 4 0.37867462889131787 1106 5 0.46305325617863957 1106 6 -0.0011586882956464706 1106 7 0.16563220380354693 1106 8 0.3401189359790635 1106 9 0.3762274413521922 1106 10-11 0.13566519018778678 1106 12-13 0.25676954292150356 1106 14-15 0.42531147228172017 1106 16-17 0.20316823607063839 1106 18-19 -0.027350549646861566 1106 20-21 0.2620370834948531 1106 22-23 0.18483255416760613 1106 24-25 0.374916090989835 1106 26-27 0.157124233510622 1106 28-29 0.21091228662643147 1106 30-31 0.08079695794702246 1106 32-33 0.3329444233046601 1106 34-35 0.3460909004535324 1106 36-37 0.24835673006526093 1106 38-39 0.30104197206295424 1106 40-41 0.4307929465275748 1106 42-43 0.09738870737962912 1106 44-45 0.319554716205225 1106 46-47 0.09448225239001573 1106 48-49 0.382443036446368 1106 50-51 -0.056328122876735165 1106 52-53 0.2457856009379853 1106 54-55 0.18807371357765135 1106 56-57 -0.3460314098446915 1106 58-59 -0.6923697148868655 1106 60-61 0.04373050386688604 1106 62-63 0.1232737210244288 1106 64-65 0.023862662708996396 1106 66-67 -0.391407930845034 1106 68-69 -0.49029003048531905 1106 70-71 -0.5987361288623667 1106 72-73 -0.041939156458219884 1106 74-75 -0.023713619597263147 1106 76-77 0.008667655826037901 1106 78-79 0.2602116448677627 1106 80-81 0.04700625440070283 1106 82-83 0.11698584023585923 1106 84-85 0.11556729594055426 1106 86-87 0.3981516006609631 1106 88-89 0.2309030851089453 1106 90-91 0.27328262214264143 1106 92-93 0.1716973322376809 1106 94-95 -0.12175127735172708 1106 96-97 0.054654540381502414 1106 98-99 0.029920791666032898 1106 100-101 -0.2920287271953228 1107 1 0.45259138706929036 1107 2 0.46093059409171744 1107 3 0.39035379316354124 1107 4 0.43271687178454243 1107 5 0.411562899254065 1107 6 0.39893856493122826 1107 7 0.3412902334763288 1107 8 0.3691870387765661 1107 9 0.4331401693107608 1107 10-11 0.4433895249565225 1107 12-13 0.44372903681767895 1107 14-15 0.5115696595329666 1107 16-17 0.5574438882946211 1107 18-19 0.534203041703357 1107 20-21 0.6006421333976064 1107 22-23 0.6223757895005875 1107 24-25 0.5818928798123224 1107 26-27 0.5448139276665955 1107 28-29 0.6326812182891928 1107 30-31 0.5622733986661004 1107 32-33 0.7025920258960312 1107 34-35 0.6252193205557504 1107 36-37 0.6040233683379839 1107 38-39 0.7435125085863703 1107 40-41 0.6931842861523094 1107 42-43 0.7395510030443475 1107 44-45 0.7051582881952143 1107 46-47 0.8177214748654578 1107 48-49 0.6725129204483764 1107 50-51 0.6673927259404167 1107 52-53 0.5669651361687613 1107 54-55 0.633068059375617 1107 56-57 0.7131411747387304 1107 58-59 0.684046472770774 1107 60-61 0.6943228665936623 1107 62-63 0.8427336142600232 1107 64-65 0.6242697985673615 1107 66-67 0.5808087527073056 1107 68-69 0.5335722249361403 1107 70-71 0.531529024581527 1107 72-73 0.5851208520028592 1107 74-75 0.8012162560832579 1107 76-77 0.8405112191487412 1107 78-79 0.7675324445744955 1107 80-81 0.628104714608174 1107 82-83 0.7814720026292328 1107 84-85 0.7183168529394983 1107 86-87 0.7568874419568914 1107 88-89 1.0123174557127435 1107 90-91 1.0472433442752127 1107 92-93 0.8766667960568917 1107 94-95 1.0038305407522188 1107 96-97 1.1592137354908907 1107 98-99 0.8875480001082003 1107 100-101 0.4633003721090887 1108 1 0.5096951575657762 1108 2 0.4402686648800298 1108 3 0.39780740877088405 1108 4 0.4370713775419688 1108 5 0.49677753113027023 1108 6 0.4399292747048946 1108 7 0.4614080407087755 1108 8 0.47490693881947976 1108 9 0.5211687676401127 1108 10-11 0.4779593565197544 1108 12-13 0.5313411993348964 1108 14-15 0.6337119224365821 1108 16-17 0.5155121194485801 1108 18-19 0.5735225832446105 1108 20-21 0.6185161282729936 1108 22-23 0.617192354013639 1108 24-25 0.5649696621458915 1108 26-27 0.7019584596965558 1108 28-29 0.4904317015030486 1108 30-31 0.6409330888815603 1108 32-33 0.6612955265639684 1108 34-35 0.7467969948503352 1108 36-37 0.8001432091197813 1108 38-39 0.7293453778689241 1108 40-41 0.7752507881804718 1108 42-43 0.671742997828872 1108 44-45 0.7686483184798973 1108 46-47 0.8469285732734093 1108 48-49 0.9020558136492625 1108 50-51 0.7493138462066113 1108 52-53 0.8067750260002171 1108 54-55 0.9184566341878622 1108 56-57 0.9744078905190676 1108 58-59 1.0274993914823511 1108 60-61 1.003597918583747 1108 62-63 0.9793068806576422 1108 64-65 0.9449062498691951 1108 66-67 0.9030176687580607 1108 68-69 0.9229249564506432 1108 70-71 0.9350331972917836 1108 72-73 0.9056701913334955 1108 74-75 0.9093796625297657 1108 76-77 0.9393888095004819 1108 78-79 0.8130032933651314 1108 80-81 0.8426521327920007 1108 82-83 1.0610562740164333 1108 84-85 1.0827156343710307 1108 86-87 1.1453262002897056 1108 88-89 1.1091429971300357 1108 90-91 1.1146181313543515 1108 92-93 1.244228225998338 1108 94-95 1.0258313481771566 1108 96-97 1.0578289049322613 1108 98-99 0.7896369155157679 1108 100-101 0.5004151065370035 1109 1 0.5732264007714143 1109 2 0.5586625423423079 1109 3 0.5504375779233825 1109 4 0.5608010814163862 1109 5 0.6005399349220681 1109 6 0.6244893477579012 1109 7 0.567338031577151 1109 8 0.5468653681798301 1109 9 0.6449628277349078 1109 10-11 0.5282117372650745 1109 12-13 0.43889696747856277 1109 14-15 0.5050124398953812 1109 16-17 0.6445261647588296 1109 18-19 0.6413402116309186 1109 20-21 0.4824562943816133 1109 22-23 0.7093608977367367 1109 24-25 0.6178006400995386 1109 26-27 0.5354723093291227 1109 28-29 0.5211430116739351 1109 30-31 0.5052986148197292 1109 32-33 0.47417607620086955 1109 34-35 0.7133328038689015 1109 36-37 0.7120975509904639 1109 38-39 0.6354689592056246 1109 40-41 0.8229000467794165 1109 42-43 0.5978766674379514 1109 44-45 0.7025480445311203 1109 46-47 0.6457351132568903 1109 48-49 0.757022809344349 1109 50-51 0.7633871601171194 1109 52-53 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-0.9906229473717296 2316 84-85 -0.9997439565646395 2316 86-87 -1.1838820334522495 2316 88-89 -1.3765386071476762 2316 90-91 -1.5989250137644646 2316 92-93 -1.7694191401069475 2316 94-95 -1.769357777653596 2316 96-97 -1.7921819772870684 2316 98-99 -1.652832296567734 2316 100-101 -1.333625327602686 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12705.0 3 4710.0 4 4927.0 5 5435.0 6 6608.0 7 8094.0 8 10293.0 9 13133.0 10 16041.0 11 19723.0 12 23621.0 13 27498.0 14 31046.0 15 35763.0 16 39501.0 17 44357.0 18 43343.0 19 42457.0 20 44870.0 21 48565.0 22 53267.0 23 58875.0 24 65804.0 25 72607.0 26 81767.0 27 92687.0 28 105395.0 29 121608.0 30 138070.0 31 161001.0 32 188800.0 33 225194.0 34 272704.0 35 332819.0 36 386963.0 37 369049.0 38 167783.0 39 8750.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.049315280070466 18.339274190661133 14.687385252491122 23.92402527677728 2 15.465845520316105 19.40410352542926 40.43665113178489 24.69339982246975 3 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23.065812165636693 26.776919019963547 27.38706028497579 22.77020852942397 48-49 22.814844856059825 26.80709821888784 27.35734694365832 23.02070998139401 50-51 22.979073830199596 26.95431208944024 27.3901798100188 22.676434270341367 52-53 23.283479942709068 26.55676649455166 27.423249024450257 22.73650453828902 54-55 22.679543592989724 26.7621910821102 27.717843843441138 22.840421481458943 56-57 22.78584093694313 26.91725128139692 27.449171673957924 22.847736107702026 58-59 22.820791498541055 26.48952241618236 27.43827738942921 23.25140869584738 60-61 22.383610935770644 26.765460229090316 27.802049755470204 23.048879079668833 62-63 22.585920193756092 26.731724606432937 27.899060755530613 22.783294444280354 64-65 22.285051651520998 26.856457521905508 27.68574396163304 23.172746864940454 66-67 22.233803594819985 26.884134914294066 27.608947028757676 23.273114462128273 68-69 22.822783262217115 26.426154425086946 27.791727636789187 22.959334675906753 70-71 22.644059214504725 26.502467172557914 27.35018015987361 23.50329345306375 72-73 22.6164700113215 26.65912392425452 27.44576499877037 23.278641065653616 74-75 22.653255530705167 27.022799896134497 27.285639294246305 23.03830527891403 76-77 22.81148562967059 26.61376913245149 27.34298592224937 23.23175931562855 78-79 22.849574397009512 26.90676608183853 27.292613242826302 22.95104627832566 80-81 23.079905145895708 26.921903975947686 26.954497705811974 23.043693172344625 82-83 22.94646836657025 26.981684129129974 27.10504326652278 22.966804237776987 84-85 22.926941290011495 27.04311631310517 27.07788050657662 22.952061890306712 86-87 23.146627260216963 27.078224027793247 26.73557872512495 23.039569986864844 88-89 22.9759962089559 27.159897300284403 26.58850789532499 23.275598595434705 90-91 22.913110412092934 27.122883623814236 26.68683356928028 23.277172394812546 92-93 23.101923024657587 26.7397057207443 26.92621512494225 23.232156129655866 94-95 22.879758045383923 26.6069865370349 27.067047746565713 23.446207671015465 96-97 23.154887639677607 26.281659900529586 27.042601032994945 23.52085142679786 98-99 22.970795717729356 26.498026657843738 26.640916717871153 23.89026090655575 100-101 23.527394839897482 25.91053196387473 26.904624539836302 23.657448656391487 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 286.0 1 212.5 2 193.0 3 272.5 4 243.5 5 221.0 6 404.0 7 959.0 8 1617.0 9 1822.5 10 1607.0 11 1459.5 12 1551.0 13 1649.5 14 1844.5 15 2139.5 16 2444.5 17 2769.0 18 3157.5 19 3701.0 20 4357.5 21 5118.0 22 6207.0 23 7965.0 24 10308.5 25 13317.0 26 17183.5 27 21992.0 28 26525.0 29 31215.5 30 36934.0 31 45123.5 32 56110.5 33 60862.0 34 61391.0 35 65132.5 36 71293.0 37 78204.5 38 83867.0 39 88664.0 40 94643.0 41 101794.0 42 106242.0 43 109446.5 44 113034.5 45 119519.0 46 124671.0 47 127881.5 48 130240.5 49 128141.0 50 124888.5 51 121418.0 52 118370.5 53 118443.0 54 120110.0 55 115356.0 56 106775.5 57 98456.5 58 91873.0 59 81568.0 60 65595.5 61 54211.5 62 46371.5 63 36190.0 64 26652.5 65 20435.0 66 15387.5 67 11870.0 68 9146.0 69 6931.5 70 5413.5 71 3925.5 72 2802.0 73 2168.0 74 1650.0 75 1191.5 76 857.0 77 622.0 78 453.5 79 302.0 80 181.0 81 131.5 82 93.0 83 51.0 84 38.5 85 30.5 86 19.0 87 13.0 88 7.5 89 4.0 90 5.5 91 6.0 92 4.5 93 5.0 94 4.0 95 2.5 96 2.5 97 1.5 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18104850416426327 2 0.047875958442132265 3 0.04489294067368355 4 0.05266060080340643 5 0.05895151946360024 6 0.06471081119476359 7 0.03609156151529033 8 0.03446714589880836 9 0.04075806455900217 10-11 0.04204282963749246 12-13 0.038291906304888636 14-15 0.034422843654722486 16-17 0.03185331349774191 18-19 0.04019690280058113 20-21 0.05069653464893278 22-23 0.04693084390163366 24-25 0.06856510643023445 26-27 0.05288211202383578 28-29 0.04484863842959768 30-31 0.053723854661467355 32-33 0.049721885279043594 34-35 0.04942653698513778 36-37 0.04657642594894668 38-39 0.025931580204930367 40-41 0.02436623424722956 42-43 0.02141275130817143 44-45 0.004061039041204926 46-47 0.011444746388850248 48-49 0.010750677898171586 50-51 0.008491263449792119 52-53 0.009037657793517874 54-55 0.010219050969141124 56-57 0.007974403935456946 58-59 0.010145213895664671 60-61 0.010514399263046937 62-63 0.003928132308947311 64-65 0.0062466164161079415 66-67 0.0037804581619944044 68-69 0.008712774670221479 70-71 0.023155306242215727 72-73 0.020098451400290564 74-75 0.02026089296193876 76-77 0.01240462834404414 78-79 0.004902781678836493 80-81 0.006276151245498523 82-83 0.005611617584210444 84-85 0.004888014264141202 86-87 0.007738125300332296 88-89 0.02993354958735413 90-91 0.03675609517657841 92-93 0.02212158721354538 94-95 0.03148412813035965 96-97 0.035692841318517486 98-99 0.021058333355484456 100-101 0.12274675094725582 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3385833.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.72376055364173 #Duplication Level Percentage of deduplicated Percentage of total 1 75.53492380903378 40.58020160154103 2 13.393754782103501 14.391257496558445 3 4.703761372127961 7.581112489730156 4 2.1360120727927474 4.590184045536221 5 1.156869961105151 3.107570239105697 6 0.7253750459484003 2.338192516807123 7 0.4752064936069859 1.787091591325417 8 0.33044514331146807 1.4202204604303308 9 0.22525007684837273 1.0891153073962239 >10 1.197786492835136 11.570355489152377 >50 0.07452741734523892 2.7426360323859478 >100 0.040063391521217635 3.997036286458707 >500 0.002763275885315819 0.9740632667738093 >1k 0.0031501344996532083 3.4228939993119814 >5k 1.1053103507555118E-4 0.40806917748661037 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7803 0.2304602737347058 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5941 0.1754664214094434 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4805 0.14191485522174307 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4706 0.13899090711207554 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4323 0.1276790674554829 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4297 0.12691116189132776 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4143 0.12236279816517825 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3724 0.10998770465052471 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3632 0.10727050034659122 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3499 0.1033423680376439 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.9534829390581285E-5 0.0 5 0.0 0.0 0.0 2.9534829390581285E-5 0.0 6 0.0 0.0 0.0 2.9534829390581285E-5 0.0 7 2.9534829390581285E-5 0.0 0.0 2.9534829390581285E-5 0.0 8 2.9534829390581285E-5 0.0 0.0 2.9534829390581285E-5 0.0 9 2.9534829390581285E-5 0.0 0.0 2.9534829390581285E-5 0.0 10-11 5.906965878116257E-5 0.0 0.0 2.9534829390581285E-5 2.9534829390581285E-5 12-13 5.906965878116257E-5 0.0 0.0 2.9534829390581285E-5 2.510460498199409E-4 14-15 5.906965878116257E-5 0.0 0.0 2.9534829390581285E-5 3.396505379916848E-4 16-17 7.383707347645321E-5 0.0 0.0 1.033719028670345E-4 4.873246849445912E-4 18-19 1.033719028670345E-4 0.0 0.0 1.3290673225761578E-4 5.906965878116257E-4 20-21 1.1813931756232514E-4 0.0 0.0 1.772089763434877E-4 6.497662465927883E-4 22-23 1.1813931756232514E-4 0.0 0.0 2.9534829390581285E-4 7.088359053739508E-4 24-25 1.1813931756232514E-4 0.0 0.0 5.75929173116335E-4 7.383707347645321E-4 26-27 1.3290673225761578E-4 0.0 0.0 8.565100523268573E-4 7.82672978850404E-4 28-29 2.06743805734069E-4 0.0 0.0 0.0028205762068005125 8.122078082409853E-4 30-31 2.510460498199409E-4 0.0 0.0 0.010071376822188218 8.26975222936276E-4 32-33 2.6581346451523156E-4 0.0 0.0 0.024691117370525952 8.565100523268573E-4 34-35 3.2488312329639413E-4 0.0 0.0 0.045690381067229245 8.565100523268573E-4 36-37 3.839527820775567E-4 0.0 0.0 0.08135368755635614 8.712774670221479E-4 38-39 4.13487611468138E-4 0.0 0.0 0.13794242066870988 8.860448817174385E-4 40-41 4.13487611468138E-4 0.0 0.0 0.1996406792656342 9.155797111080198E-4 42-43 4.4302244085871927E-4 0.0 0.0 0.26488311738942827 9.303471258033105E-4 44-45 4.7255727024930055E-4 0.0 0.0 0.3456165735285822 9.598819551938918E-4 46-47 5.168595143351725E-4 0.0 0.0 0.4411026769483315 0.0010041841992797637 48-49 5.316269290304631E-4 0.0 0.0 0.5448733000121389 0.0010041841992797637 50-51 5.316269290304631E-4 0.0 0.0 0.660575994149741 0.001033719028670345 52-53 5.75929173116335E-4 0.0 0.0 0.7715826504142407 0.0010927886874515075 54-55 5.906965878116257E-4 0.0 0.0 0.8933990542356933 0.0011223235168420888 56-57 5.906965878116257E-4 0.0 0.0 1.0373961149294724 0.0011223235168420888 58-59 5.906965878116257E-4 0.0 0.0 1.1911692041515338 0.0011223235168420888 60-61 5.906965878116257E-4 0.0 0.0 1.3628847022283734 0.0011223235168420888 62-63 5.906965878116257E-4 0.0 0.0 1.5287966063299638 0.0011223235168420888 64-65 5.906965878116257E-4 0.0 0.0 1.704573143448008 0.0011223235168420888 66-67 6.20231417202207E-4 0.0 0.0 1.9147282219766892 0.0011370909315373795 68-69 6.20231417202207E-4 0.0 0.0 2.1484077921149685 0.0011813931756232514 70-71 6.497662465927883E-4 0.0 0.0 2.3945953625001586 0.0011813931756232514 72-73 6.497662465927883E-4 0.0 0.0 2.648816406479587 0.0012552302490997046 74-75 6.497662465927883E-4 0.0 0.0 2.9108789476622148 0.0013290673225761578 76-77 6.497662465927883E-4 0.0 0.0 3.1925969178042743 0.0013733695666620297 78-79 6.497662465927883E-4 0.0 0.0 3.5076597103283005 0.0013881369813573204 80-81 7.088359053739508E-4 0.0 0.0 3.843352581181647 0.001402904396052611 82-83 7.383707347645321E-4 0.0 0.0 4.185277301036407 0.0015948807870913894 84-85 7.82672978850404E-4 0.0 0.0 4.536771305613715 0.0015948807870913894 86-87 8.122078082409853E-4 0.0 0.0 4.907226670659776 0.00160964820178668 88-89 8.26975222936276E-4 0.0 0.0 5.29993652965164 0.001653950445872552 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5335 0.0 45.49728 1 GTATCAA 9320 0.0 39.065666 1 ATCAACG 11725 0.0 30.560871 3 TATCAAC 11920 0.0 30.180529 2 TCAACGC 12000 0.0 29.781313 4 CAACGCA 12155 0.0 29.283382 5 AACGCAG 12765 0.0 27.953934 6 ACGCAGA 14430 0.0 24.495451 7 CGCAGAG 14550 0.0 24.064861 8 GTACATG 9920 0.0 22.06967 1 TACATGG 9555 0.0 21.585709 2 GCAGAGT 16140 0.0 21.309603 9 TAAGGTG 2365 0.0 21.299486 5 AGAGTAC 13545 0.0 20.902025 10-11 GTGTAAG 1435 0.0 20.894806 1 AGGACCG 1190 0.0 19.96721 5 GAGTACT 9740 0.0 19.921505 12-13 GTAAGGT 2315 0.0 19.912601 4 ACATGGG 9690 0.0 19.813665 3 GTATAGC 1870 0.0 19.342903 1 >>END_MODULE