FastQCFastQC Report
Thu 26 May 2016
SRR938386_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938386_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3385833
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79160.2337977094558414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64310.18993848781082823No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47580.14052671824038573No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA47480.14023136994647994No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA46890.13848881501243565No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC43110.12732464950279593No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA42790.1263795349622973No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA41330.12206744987127245No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG39080.11542211325839165No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA34760.10266306696166054No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53400.046.1311151
GTATCAA92600.038.7227131
TATCAAC116550.030.3162542
ATCAACG116800.030.1149673
TCAACGC118550.029.7105164
CAACGCA121350.029.0249845
AACGCAG125400.028.141346
GTAAGGT22800.024.8087944
ACGCAGA143150.024.6187537
CGCAGAG143350.024.4518768
TAAGGTG26600.022.8729345
GTACATG92800.022.8555831
TACATGG89750.022.8371472
AGGTAAG23000.022.5370832
ACATGGG88200.022.3652153
GGTAAGG24650.021.597063
GCAGAGT163050.021.177139
AGAGTAC136800.020.97028510-11
GAGTACT101250.020.31170512-13
AAGGTAA23550.020.1936341
Produced by FastQC (version 0.11.5)