FastQCFastQC Report
Thu 26 May 2016
SRR938385_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938385_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3392118
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79590.23463216786680183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61510.181332135261804No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA47900.14120971027540904No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA46930.13835013994206571No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43980.12965350851591836No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA42300.1247008506189938No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA41920.1235806065708799No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA38420.11326256928562038No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC35930.10592202275982145No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG34320.10117572560860205No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51250.047.2412951
GTATCAA91550.040.189341
ATCAACG114650.031.5063343
TATCAAC114850.031.4100882
TCAACGC116100.031.1128444
CAACGCA118600.030.4169375
AACGCAG125250.029.0253836
ACGCAGA141400.025.333467
CGCAGAG142650.025.044868
TAAGGTG22400.024.8253965
TACATGG93950.022.1582622
GTACATG95500.022.0083331
GCAGAGT159100.021.646899
AGAGTAC133950.020.94089910-11
ACATGGG95800.020.8869463
GTAAGGT23600.020.7435744
GAGTACT94950.020.51149712-13
GTGTAAG15850.020.4470941
GGTAAGG23650.020.0968153
TAAGGTA13250.019.7290064
Produced by FastQC (version 0.11.5)