FastQCFastQC Report
Thu 26 May 2016
SRR938385_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938385_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3392118
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79690.23492696893209494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64550.19029408764671513No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA46910.13829117972900706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46380.13672873408295347No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA44840.13218879767743927No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA42960.12664653764992845No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA41290.12172335985953318No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC41130.12125167815506417No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG37480.11049143927186494No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA34830.10267921104159701No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55750.046.0752221
GTATCAA92600.037.910891
TATCAAC121500.028.541042
ATCAACG121700.028.4794223
TCAACGC122950.028.151214
CAACGCA126600.027.3020295
AACGCAG131450.026.3040776
TAAGGTG21800.023.771925
ACGCAGA148200.023.3311167
CGCAGAG149250.023.103338
GTACATG95350.021.651481
TACATGG91100.021.3039632
GTAAGGT23350.020.7686144
AGAGTAC141650.020.70487810-11
ACATGGG92950.020.1530653
GCAGAGT169400.020.0748339
GAGTACT103600.019.41538212-13
AGTACTT110850.019.13101812-13
AGGTAAG22750.019.027362
GTGTAGC21550.018.9832131
Produced by FastQC (version 0.11.5)