##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938385_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3392118 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.799959199532562 31.0 31.0 34.0 30.0 34.0 2 32.03439561949201 31.0 31.0 34.0 30.0 34.0 3 32.10589637506714 33.0 31.0 34.0 30.0 34.0 4 35.77371512429697 37.0 35.0 37.0 35.0 37.0 5 35.641228872344655 37.0 35.0 37.0 33.0 37.0 6 35.68093857583964 37.0 35.0 37.0 33.0 37.0 7 35.616320540735906 37.0 35.0 37.0 33.0 37.0 8 35.59799541171622 37.0 35.0 37.0 33.0 37.0 9 37.17685911869811 39.0 37.0 39.0 33.0 39.0 10-11 37.10657146950666 39.0 37.0 39.0 33.0 39.0 12-13 36.99450387633921 39.0 37.0 39.0 33.0 39.0 14-15 38.051500861703516 40.0 37.0 41.0 33.0 41.0 16-17 37.96614239245215 40.0 37.0 41.0 33.0 41.0 18-19 37.95587388174586 40.0 37.0 41.0 33.0 41.0 20-21 37.94277911322661 40.0 37.0 41.0 32.5 41.0 22-23 37.978493083082604 40.0 37.0 41.0 33.0 41.0 24-25 37.8954393685597 40.0 37.0 41.0 32.5 41.0 26-27 37.71607134539541 40.0 37.0 41.0 32.0 41.0 28-29 37.582603848097264 39.5 37.0 41.0 32.0 41.0 30-31 37.33698385492485 39.0 36.0 41.0 31.5 41.0 32-33 37.11594349017339 39.0 36.0 41.0 31.0 41.0 34-35 36.942712635586375 39.0 36.0 40.5 30.5 41.0 36-37 36.93970021090068 39.0 36.0 41.0 30.5 41.0 38-39 36.77744701098251 39.0 35.0 41.0 30.0 41.0 40-41 36.59515809886331 39.0 35.0 40.5 30.0 41.0 42-43 36.36294064652232 39.0 35.0 40.0 30.0 41.0 44-45 36.0843486282022 38.5 35.0 40.0 29.5 41.0 46-47 35.73190850082456 38.0 35.0 40.0 28.5 41.0 48-49 35.333346157179676 38.0 34.0 40.0 27.0 41.0 50-51 34.946498913068474 38.0 34.0 40.0 26.0 41.0 52-53 34.41334514306401 38.0 33.0 40.0 24.0 41.0 54-55 34.07402159948445 37.0 33.0 40.0 24.0 41.0 56-57 33.69514238596653 37.0 32.5 40.0 23.0 41.0 58-59 33.20699338879131 36.5 31.5 39.0 22.0 41.0 60-61 32.77350994275553 36.0 31.0 39.0 20.5 40.0 62-63 32.27478510476345 35.5 31.0 39.0 18.5 40.0 64-65 31.75504832084261 35.0 30.0 38.0 17.5 40.0 66-67 31.241942792084473 35.0 29.5 38.0 13.5 40.0 68-69 30.654003192105932 34.0 29.0 37.5 9.5 39.5 70-71 30.060565847060744 34.0 28.5 37.0 7.5 39.0 72-73 29.45858457754123 34.0 28.0 36.0 6.5 39.0 74-75 28.824405725272527 33.0 26.5 36.0 2.0 38.5 76-77 27.556806838677193 31.5 26.0 34.5 2.0 36.0 78-79 28.158173890177167 33.0 26.0 35.0 2.0 37.0 80-81 27.830989370063186 33.0 26.0 35.0 2.0 36.5 82-83 27.311485921185525 32.5 26.0 35.0 2.0 36.0 84-85 26.696955854719675 32.0 25.0 35.0 2.0 36.0 86-87 26.03172560624365 32.0 23.5 34.5 2.0 35.0 88-89 25.2454858586877 31.0 20.0 34.0 2.0 35.0 90-91 24.666940389455792 31.0 18.5 34.0 2.0 35.0 92-93 23.868671284430555 31.0 10.0 34.0 2.0 35.0 94-95 23.045871487961207 30.0 2.0 34.0 2.0 35.0 96-97 22.03273308888429 29.5 2.0 34.0 2.0 35.0 98-99 20.820769796333735 29.0 2.0 34.0 2.0 35.0 100-101 17.70412571142867 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.31377888824422584 1101 2 0.4061965109906538 1101 3 0.3675097521238655 1101 4 0.23832953399433165 1101 5 0.25678139439078507 1101 6 0.28762447974702354 1101 7 0.32985885990132857 1101 8 0.3248984701466995 1101 9 0.47313985380052515 1101 10-11 0.4379139402200707 1101 12-13 0.3763190661371212 1101 14-15 0.5900347526358019 1101 16-17 0.6589050547092228 1101 18-19 0.6949630352825551 1101 20-21 0.6573716875975961 1101 22-23 0.5910264623588688 1101 24-25 0.6386503887980979 1101 26-27 0.6325088871542306 1101 28-29 0.6166637251693032 1101 30-31 0.8230488060418253 1101 32-33 0.6412482307366716 1101 34-35 0.5653360153382891 1101 36-37 0.6850573726752884 1101 38-39 0.9084280292213123 1101 40-41 1.0562268765673437 1101 42-43 0.9324962939706225 1101 44-45 1.0431763250067974 1101 46-47 0.9760004063362473 1101 48-49 1.1433060223229745 1101 50-51 1.2124275363779518 1101 52-53 1.2521206267954383 1101 54-55 1.3345359132554293 1101 56-57 1.5077332142430677 1101 58-59 1.6541468039037923 1101 60-61 1.6234551481959514 1101 62-63 1.7640307740912817 1101 64-65 1.8147260864784442 1101 66-67 1.9062011486623192 1101 68-69 1.9865857477929545 1101 70-71 2.053085187459885 1101 72-73 2.129482705258095 1101 74-75 2.3878933786033407 1101 76-77 2.2604790239109853 1101 78-79 2.4380581189095345 1101 80-81 2.6695556252174164 1101 82-83 2.702921996706227 1101 84-85 2.9024811791813683 1101 86-87 2.982203346701347 1101 88-89 3.2699638978920973 1101 90-91 3.4974632652594124 1101 92-93 3.8323618717349603 1101 94-95 3.984002529192818 1101 96-97 4.260823869741618 1101 98-99 4.629946334284792 1101 100-101 5.1497482016047655 1102 1 0.28691661727554774 1102 2 0.2655597856822425 1102 3 0.2593242093835144 1102 4 0.2106259607408134 1102 5 0.2041657837622779 1102 6 0.1311867835648215 1102 7 0.24191676558787378 1102 8 0.31232428589967043 1102 9 0.31769201089228005 1102 10-11 0.3092741216201702 1102 12-13 0.35776825950890156 1102 14-15 0.5080497537307735 1102 16-17 0.4059615916827184 1102 18-19 0.5551437054044612 1102 20-21 0.6174337815460618 1102 22-23 0.5167428923902548 1102 24-25 0.405500615091988 1102 26-27 0.4733530637364609 1102 28-29 0.44412987233332757 1102 30-31 0.4340155280653306 1102 32-33 0.22414086888544205 1102 34-35 0.3757630994320209 1102 36-37 0.4829555313000071 1102 38-39 0.658835996956185 1102 40-41 0.6185194252935204 1102 42-43 0.651534681809153 1102 44-45 0.5796787613160177 1102 46-47 0.4551098760055723 1102 48-49 0.5683980910871185 1102 50-51 0.7018768118725163 1102 52-53 0.7904513443660548 1102 54-55 0.7201810333371839 1102 56-57 0.9717455509099793 1102 58-59 0.9534576568857602 1102 60-61 0.9474584969805306 1102 62-63 1.19327068211377 1102 64-65 1.2634773639439487 1102 66-67 1.3306487817012282 1102 68-69 1.4604995827444078 1102 70-71 1.5321572456393113 1102 72-73 1.4702217190547238 1102 74-75 1.5312420263148532 1102 76-77 1.5169221949453693 1102 78-79 1.8791962038062415 1102 80-81 1.9653525811996069 1102 82-83 2.0812179945892844 1102 84-85 2.3266699885835145 1102 86-87 2.3712801041288962 1102 88-89 2.474744584438561 1102 90-91 2.721462827271239 1102 92-93 3.107223312351067 1102 94-95 3.261178417943821 1102 96-97 3.7399120787349744 1102 98-99 4.111714711538607 1102 100-101 4.4486057368263126 1103 1 0.23918404356097156 1103 2 0.2157792774044225 1103 3 0.2617924643439551 1103 4 0.22369652313253852 1103 5 0.14293938046186128 1103 6 0.2125345413202595 1103 7 0.19475015547590147 1103 8 0.22348496669906126 1103 9 0.23862828864128716 1103 10-11 0.2861777970796098 1103 12-13 0.334597148834348 1103 14-15 0.43291491544741234 1103 16-17 0.46167181438912763 1103 18-19 0.39154191589506127 1103 20-21 0.39269363545636793 1103 22-23 0.3881025266302487 1103 24-25 0.34143055276277323 1103 26-27 0.34144385187070014 1103 28-29 0.35386983982926523 1103 30-31 0.4561364181765981 1103 32-33 0.5381224380101486 1103 34-35 0.552177115469739 1103 36-37 0.6406210877088441 1103 38-39 0.7260079648807505 1103 40-41 0.5813552261972106 1103 42-43 0.7979673608940345 1103 44-45 0.9229236758589039 1103 46-47 0.9466655615376922 1103 48-49 1.0316879666072722 1103 50-51 0.9111759653381242 1103 52-53 1.0984570772255253 1103 54-55 1.067503823685982 1103 56-57 1.0627239321362083 1103 58-59 1.2893034322505414 1103 60-61 1.2275167185439457 1103 62-63 1.421123226181777 1103 64-65 1.462302217050592 1103 66-67 1.533963079917072 1103 68-69 1.4199235732432207 1103 70-71 1.468197509600877 1103 72-73 1.643344609416019 1103 74-75 1.7380107741940485 1103 76-77 1.6415195999781353 1103 78-79 1.8991673162667233 1103 80-81 2.1934037215207134 1103 82-83 2.436177810994902 1103 84-85 2.383666175606894 1103 86-87 2.4937007638542177 1103 88-89 2.7474753275671624 1103 90-91 2.944566786694736 1103 92-93 3.1034869524489324 1103 94-95 3.2890941395518816 1103 96-97 3.7874202047507275 1103 98-99 4.225161437022148 1103 100-101 4.434317924064004 1104 1 0.27687271267904734 1104 2 0.30684822917260135 1104 3 0.24860113196255895 1104 4 0.13711132767321033 1104 5 0.16722551343077896 1104 6 0.19508641787297165 1104 7 0.19969499883239195 1104 8 0.20790513184523718 1104 9 0.3067290223592565 1104 10-11 0.3001810158284357 1104 12-13 0.36193930404994745 1104 14-15 0.3622810996709447 1104 16-17 0.48528910702776074 1104 18-19 0.46033717688805353 1104 20-21 0.5230227180192344 1104 22-23 0.44841871034259384 1104 24-25 0.5192901103509371 1104 26-27 0.4704053069700862 1104 28-29 0.5688916332636325 1104 30-31 0.5588996177731005 1104 32-33 0.5367689958743611 1104 34-35 0.5915482581681673 1104 36-37 0.664903514601157 1104 38-39 0.7330619492112831 1104 40-41 0.6529190542079917 1104 42-43 0.7572645013364507 1104 44-45 0.7500188870967506 1104 46-47 0.6756132146966323 1104 48-49 0.7921753966178002 1104 50-51 0.8719353872924103 1104 52-53 1.079978749679917 1104 54-55 1.2306031431310345 1104 56-57 1.2986544299943432 1104 58-59 1.4065628013825844 1104 60-61 1.4076218177645572 1104 62-63 1.4242651697676223 1104 64-65 1.5205641267620464 1104 66-67 1.7836167108497776 1104 68-69 1.8120921221992141 1104 70-71 1.7277288144465501 1104 72-73 1.8156887582452406 1104 74-75 2.057911425781956 1104 76-77 2.002148244286797 1104 78-79 2.177955224744526 1104 80-81 2.448294081291298 1104 82-83 2.645935527301983 1104 84-85 2.8574938200416433 1104 86-87 2.977009276413984 1104 88-89 3.030309249328905 1104 90-91 3.2371597453195875 1104 92-93 3.490060390699732 1104 94-95 3.602923598686207 1104 96-97 3.868324069975632 1104 98-99 4.161146972247767 1104 100-101 4.468652338265347 1105 1 0.22473229224435087 1105 2 0.17294550018198152 1105 3 0.2027956976687051 1105 4 0.15667400142321952 1105 5 0.0984681494116657 1105 6 0.17413754218640065 1105 7 0.22109614015580803 1105 8 0.22851176570080156 1105 9 0.2655806907473419 1105 10-11 0.29482133722536474 1105 12-13 0.39455801356953657 1105 14-15 0.4247400704705271 1105 16-17 0.3765348223683205 1105 18-19 0.3788745953963044 1105 20-21 0.340860623629915 1105 22-23 0.2628419573027543 1105 24-25 0.28549029895150824 1105 26-27 0.4353063243431521 1105 28-29 0.3900866943627861 1105 30-31 0.5661631901325421 1105 32-33 0.5110923637626854 1105 34-35 0.44564660409461254 1105 36-37 0.5291779190370676 1105 38-39 0.5567746523050658 1105 40-41 0.6673131003436552 1105 42-43 0.6913689814780497 1105 44-45 0.6595560805748804 1105 46-47 0.7879546266524713 1105 48-49 0.8123142863575339 1105 50-51 0.7578243236921978 1105 52-53 0.8674662984891981 1105 54-55 1.0407659285384696 1105 56-57 0.9886366685418011 1105 58-59 1.237337938289528 1105 60-61 1.2868582345824322 1105 62-63 1.2962541869903959 1105 64-65 1.4325815933475212 1105 66-67 1.4483998630767374 1105 68-69 1.5263805961232855 1105 70-71 1.6133388931825934 1105 72-73 1.7235541534712517 1105 74-75 1.8413140038676126 1105 76-77 1.8342656389524805 1105 78-79 2.0893352148693403 1105 80-81 2.4233116119960663 1105 82-83 2.4757021126555863 1105 84-85 2.6245904943740364 1105 86-87 2.886024694040909 1105 88-89 3.0242301685953628 1105 90-91 3.3536651948468794 1105 92-93 3.6102499224287783 1105 94-95 3.6387414116792307 1105 96-97 3.9637609408561154 1105 98-99 4.397690068376473 1105 100-101 4.773271500295618 1106 1 0.16149945680330546 1106 2 0.1120941232488093 1106 3 0.07653916753686474 1106 4 0.08480166701770031 1106 5 0.017529943515661728 1106 6 0.0899347300891904 1106 7 0.13355369598449585 1106 8 0.0056699670707303085 1106 9 0.13732567731501177 1106 10-11 0.18226806211309565 1106 12-13 0.11565962875889113 1106 14-15 0.02602708292801026 1106 16-17 -0.08321600346794611 1106 18-19 0.1417423294408664 1106 20-21 0.3348010584808563 1106 22-23 0.189260577645598 1106 24-25 0.10494776872110378 1106 26-27 -0.008527002492847657 1106 28-29 0.1803747912628637 1106 30-31 0.2224897713824987 1106 32-33 0.22160969167454425 1106 34-35 0.209428011065107 1106 36-37 0.21635349649723423 1106 38-39 0.22201435629121846 1106 40-41 0.2654643500515803 1106 42-43 0.43921952699705713 1106 44-45 -0.0436463473212072 1106 46-47 -0.1681104918822811 1106 48-49 0.34996121509006173 1106 50-51 0.2048867052536778 1106 52-53 0.3082314103794985 1106 54-55 0.49535639637872464 1106 56-57 0.32105081234089994 1106 58-59 0.8124626197666913 1106 60-61 0.7304396363060235 1106 62-63 0.8256269388192692 1106 64-65 0.41808848261055687 1106 66-67 0.5832376613076491 1106 68-69 0.5034338348259482 1106 70-71 0.7422555318371487 1106 72-73 1.0792683666815428 1106 74-75 1.4787495116517952 1106 76-77 1.4103097551464714 1106 78-79 1.7902726459883525 1106 80-81 1.626327455940288 1106 82-83 2.023473979081089 1106 84-85 2.070286350763727 1106 86-87 2.2978765262781558 1106 88-89 2.6585311395429443 1106 90-91 2.5133218460498803 1106 92-93 2.1839362402349423 1106 94-95 2.528084010953048 1106 96-97 3.0551853609931605 1106 98-99 3.4297377622748364 1106 100-101 3.712794762361728 1107 1 0.1683470856005016 1107 2 0.18875607693713192 1107 3 0.16565163037569874 1107 4 0.13180703930358817 1107 5 0.03353265811477968 1107 6 0.07167168030903781 1107 7 0.0692762649739933 1107 8 0.17283161188027663 1107 9 0.23463112885630721 1107 10-11 0.234791463630593 1107 12-13 0.21730904382344107 1107 14-15 0.3305475702154297 1107 16-17 0.3859825617680386 1107 18-19 0.2967987996135051 1107 20-21 0.42699789143264155 1107 22-23 0.2744554021092398 1107 24-25 0.34599289097159414 1107 26-27 0.3341134482019257 1107 28-29 0.3843764688634721 1107 30-31 0.3584962891585306 1107 32-33 0.43382783929177293 1107 34-35 0.38276368934594984 1107 36-37 0.42101177532009615 1107 38-39 0.48859417393452986 1107 40-41 0.5390540165540401 1107 42-43 0.5326427611285993 1107 44-45 0.5555350224290194 1107 46-47 0.6326605867163622 1107 48-49 0.6504098435067007 1107 50-51 0.7570654488378139 1107 52-53 0.6856773772886768 1107 54-55 0.6872789277822022 1107 56-57 0.777611296504908 1107 58-59 0.6885996427069827 1107 60-61 0.8305932120604425 1107 62-63 1.0194895839566769 1107 64-65 1.117953309838633 1107 66-67 1.18856098666609 1107 68-69 1.2150490698528138 1107 70-71 1.28306844378994 1107 72-73 1.4273010822812005 1107 74-75 1.3709077556506664 1107 76-77 1.4746408747888644 1107 78-79 1.7208450862007503 1107 80-81 2.0271671397751376 1107 82-83 2.0817680528511 1107 84-85 2.2632217227845572 1107 86-87 2.4792497788007424 1107 88-89 2.66839977870368 1107 90-91 2.7003622938472986 1107 92-93 3.0391469945813157 1107 94-95 3.083695245082364 1107 96-97 3.3079940246588357 1107 98-99 3.6636130045403092 1107 100-101 3.7846401811294754 1108 1 0.19105753671342285 1108 2 0.20715372047752822 1108 3 0.22228532359523712 1108 4 0.150982523540641 1108 5 0.10879390953960666 1108 6 0.05041763801537513 1108 7 0.12872720285702854 1108 8 0.16636062779695493 1108 9 0.11261827567851412 1108 10-11 0.20904556858621248 1108 12-13 0.33965910003807664 1108 14-15 0.33147027333670565 1108 16-17 0.3605420377938273 1108 18-19 0.4074563886973479 1108 20-21 0.42745926226202613 1108 22-23 0.2708472525400083 1108 24-25 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1.0560099492962784 2316 78-79 1.167929531881672 2316 80-81 1.337029938929593 2316 82-83 1.7294466957127206 2316 84-85 1.7661896794189964 2316 86-87 1.8733195120893562 2316 88-89 2.1132976979916727 2316 90-91 2.2634195985701133 2316 92-93 1.9767719216365158 2316 94-95 2.7798884798117776 2316 96-97 2.963517464577201 2316 98-99 3.308304788128684 2316 100-101 3.7258562056557274 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 14.0 4 63.0 5 98.0 6 248.0 7 668.0 8 1467.0 9 2721.0 10 4489.0 11 6929.0 12 10071.0 13 14232.0 14 18557.0 15 24118.0 16 29518.0 17 36471.0 18 35955.0 19 33300.0 20 33635.0 21 36166.0 22 39474.0 23 44578.0 24 51398.0 25 59675.0 26 69337.0 27 81913.0 28 97480.0 29 115839.0 30 135683.0 31 159859.0 32 189038.0 33 229569.0 34 283316.0 35 353762.0 36 442449.0 37 483093.0 38 253140.0 39 13788.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.92675774657271 19.141638264998836 15.271100933253107 23.660503055175344 2 16.05150369021103 20.228473375740876 39.254236428328745 24.465786505719358 3 19.559088720858487 23.24295991493203 29.849259060213534 27.348692303995946 4 13.534994950057753 16.832963947598518 36.210709651020395 33.42133145132333 5 14.07006229773009 36.93968265959459 34.25655120639697 14.733703836278341 6 33.266708292577086 36.047566741487174 16.652987897237065 14.032737068698673 7 29.209891872865274 32.044345155445654 20.098180546785223 18.64758242490385 8 26.501908247295642 35.37040869450886 19.197180050929834 18.930503007265667 9 25.95007013317343 16.80422084373244 19.694391527653224 37.5513174954409 10-11 25.153797715763424 26.3108329368259 27.28236458755267 21.253004759857998 12-13 26.150903358904376 23.275679088993957 27.494297073391905 23.07912047870976 14-15 22.4805121755788 25.07946362714976 25.655121667347657 26.784902529923784 16-17 22.525955759793735 28.133234162254965 26.57755420064986 22.76325587730144 18-19 22.53972296954292 26.692998297818647 28.555934669725524 22.211344062912904 20-21 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26.47329829935698 28.30176998181478 22.631996876216544 46-47 23.044727384805327 26.7837351694731 27.506617181337965 22.66492026438361 48-49 22.784835315280898 26.800084784786378 27.437282547364212 22.977797352568512 50-51 23.010063329164847 26.952614855969042 27.46987280513237 22.567449009733743 52-53 23.33567110578111 26.54114626908616 27.438815512903737 22.684367112228998 54-55 22.714864282433574 26.759888659533658 27.75123094184813 22.774016116184637 56-57 22.85600029244266 26.901496351247218 27.451256117859106 22.79124723845102 58-59 22.875265542059566 26.545538804959023 27.435307380226746 23.143888272754662 60-61 22.427462723879298 26.817964469396404 27.771159493861948 22.983413312862346 62-63 22.60720293338852 26.845867390226402 27.78433710148055 22.762592574904527 64-65 22.309719178401224 26.888628284747167 27.613691504835625 23.187961032015984 66-67 22.202391902154176 26.892346125363787 27.61906151683671 23.286200455645332 68-69 22.852949938143286 26.463825576591116 27.71623060372243 22.966993881543175 70-71 22.617181949448693 26.548133054333604 27.321528909076864 23.513156087140835 72-73 22.557547018934336 26.715967238752786 27.401777798086297 23.324707944226578 74-75 22.63109655973053 27.021937326472724 27.11590516603491 23.231060947761843 76-77 22.67569996090938 26.655086880821955 27.423736438413993 23.245476719854675 78-79 22.70444306477546 26.876158199685268 27.39393205071286 23.025466684826412 80-81 22.89481422739631 26.875675739416454 27.23105847601093 22.998451557176306 82-83 22.777790061274995 27.002934773155747 27.25313487132545 22.966140294243807 84-85 22.715137828688682 27.03357107532247 27.20792338993494 23.043367706053907 86-87 22.93790430683792 27.05729036921692 26.894970653621343 23.109834670323824 88-89 22.700797569894785 27.243462894036806 26.65613047193397 23.39960906413444 90-91 22.57357225581098 27.17303680429517 26.927642408125607 23.32574853176824 92-93 22.716558698026287 26.813181111528912 27.150198335166753 23.32006185527805 94-95 22.490697552384674 26.621007877674067 27.264101661557767 23.624192908383492 96-97 22.644613188574215 26.325499289824233 27.272105510480472 23.757782011121076 98-99 22.414019795301932 26.616187880256525 26.938714985740475 24.03107733870107 100-101 22.489339924341763 26.242329226531325 27.31884651849476 23.94948433063215 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 308.0 1 225.0 2 188.5 3 255.5 4 233.0 5 227.5 6 425.5 7 995.5 8 1618.0 9 1899.0 10 1724.0 11 1477.0 12 1547.5 13 1696.5 14 1925.0 15 2243.0 16 2543.5 17 2880.0 18 3253.5 19 3701.0 20 4270.0 21 5109.5 22 6312.5 23 7909.0 24 10273.0 25 13308.0 26 17204.5 27 21766.5 28 26258.0 29 31385.0 30 37354.0 31 45615.0 32 57724.5 33 62372.0 34 61740.5 35 65846.0 36 72052.0 37 78618.5 38 84153.5 39 88982.5 40 95413.0 41 103263.5 42 107432.0 43 109973.0 44 114385.5 45 121056.5 46 125111.5 47 129680.0 48 132511.5 49 129557.5 50 126323.5 51 121795.0 52 118233.5 53 119264.0 54 122155.5 55 116546.0 56 106533.5 57 97724.0 58 90539.5 59 80250.0 60 64162.0 61 52819.0 62 45417.5 63 34748.5 64 24561.5 65 18408.0 66 13886.5 67 10911.0 68 8444.0 69 6305.0 70 4799.0 71 3390.0 72 2437.5 73 1958.5 74 1438.5 75 1012.0 76 754.5 77 521.0 78 346.5 79 222.5 80 134.0 81 91.0 82 51.0 83 32.5 84 18.5 85 11.5 86 9.0 87 4.5 88 2.5 89 1.5 90 2.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.4740053264656477E-4 2 0.04498664256373157 3 2.0636074570519068E-4 4 0.0 5 0.09996704124090024 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0011644642079078618 26-27 0.010686538616875947 28-29 0.0 30-31 0.0 32-33 3.39021225087099E-4 34-35 0.014120971027540905 36-37 0.002874310386608013 38-39 0.011349841013785488 40-41 0.0062645226374790025 42-43 0.005100058429571141 44-45 0.002284708256021754 46-47 4.422015979396943E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.7688063917587773E-4 68-69 1.326604793819083E-4 70-71 0.0 72-73 1.4740053264656477E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.4740053264656477E-5 82-83 9.728435154673275E-4 84-85 0.003994554434721905 86-87 0.0013855650068777087 88-89 4.422015979396943E-5 90-91 8.107029295561063E-4 92-93 1.9162069244053423E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 6.48562343644885E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3392118.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.56519871233546 #Duplication Level Percentage of deduplicated Percentage of total 1 72.71116061133299 36.766542849165916 2 14.802591153413095 14.969919262595843 3 5.224268078062772 7.924984604812647 4 2.44407930120576 4.943414221367009 5 1.3348469934309661 3.374840173670017 6 0.8121811170793574 2.464085974531458 7 0.5299884054869485 1.8759278327076956 8 0.35673520261824765 1.4430709126461556 9 0.27584877737342445 1.255351341819779 >10 1.3643831799049173 12.38383171956299 >50 0.09287105056972933 3.2220875616444293 >100 0.044034400475435005 4.23949670250223 >500 0.003739588827861529 1.2814599774968658 >1k 0.0031552780678098372 3.4288183998886197 >5k 1.1686215065962358E-4 0.42616846558834864 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7969 0.23492696893209494 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6455 0.19029408764671513 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4691 0.13829117972900706 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4638 0.13672873408295347 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4484 0.13218879767743927 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4296 0.12664653764992845 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4129 0.12172335985953318 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4113 0.12125167815506417 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3748 0.11049143927186494 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3483 0.10267921104159701 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 2.9480106529312954E-5 5 0.0 0.0 0.0 0.0 2.9480106529312954E-5 6 0.0 0.0 0.0 0.0 2.9480106529312954E-5 7 8.844031958793886E-5 0.0 0.0 0.0 2.9480106529312954E-5 8 8.844031958793886E-5 0.0 0.0 0.0 2.9480106529312954E-5 9 8.844031958793886E-5 0.0 0.0 5.896021305862591E-5 2.9480106529312954E-5 10-11 1.4740053264656477E-4 0.0 0.0 5.896021305862591E-5 2.9480106529312954E-5 12-13 1.4740053264656477E-4 0.0 0.0 5.896021305862591E-5 8.844031958793886E-5 14-15 1.6214058591122125E-4 0.0 0.0 5.896021305862591E-5 8.844031958793886E-5 16-17 2.0636074570519068E-4 0.0 0.0 7.370026632328239E-5 8.844031958793886E-5 18-19 2.3584085223450364E-4 0.0 0.0 1.0318037285259534E-4 8.844031958793886E-5 20-21 2.3584085223450364E-4 0.0 0.0 1.1792042611725182E-4 1.0318037285259534E-4 22-23 2.653209587638166E-4 0.0 0.0 1.7688063917587773E-4 1.326604793819083E-4 24-25 3.09541118557786E-4 0.0 0.0 3.6850133161641193E-4 1.4740053264656477E-4 26-27 3.242811718224425E-4 0.0 0.0 6.48562343644885E-4 1.6214058591122125E-4 28-29 3.5376127835175545E-4 0.0 0.0 0.0025205491082562577 1.7688063917587773E-4 30-31 4.2746154467503784E-4 0.0 0.0 0.009522074408968084 1.916206924405342E-4 32-33 4.422015979396943E-4 0.0 0.0 0.024114727140977996 2.0636074570519068E-4 34-35 5.011618109983202E-4 0.0 0.0 0.04690284948813691 2.3584085223450364E-4 36-37 5.601220240569461E-4 0.0 0.0 0.0832076006789858 2.9480106529312954E-4 38-39 6.19082237115572E-4 0.0 0.0 0.13964726462935545 3.5376127835175545E-4 40-41 6.19082237115572E-4 0.0 0.0 0.2018208092996765 3.5376127835175545E-4 42-43 6.78042450174198E-4 0.0 0.0 0.2648640171126122 3.979814381457249E-4 44-45 7.370026632328239E-4 0.0 0.0 0.3473493551816299 4.8642175773366375E-4 46-47 8.401830360854191E-4 0.0 0.0 0.4427912000702806 5.601220240569461E-4 48-49 8.549230893500757E-4 0.0 0.0 0.5435984243472662 5.601220240569461E-4 50-51 8.549230893500757E-4 0.0 0.0 0.6582023384799703 5.896021305862591E-4 52-53 8.696631426147322E-4 0.0 0.0 0.7696961013738319 6.19082237115572E-4 54-55 8.844031958793886E-4 0.0 0.0 0.8937041694893868 6.338222903802286E-4 56-57 8.844031958793886E-4 0.0 0.0 1.036918527008789 6.48562343644885E-4 58-59 8.844031958793886E-4 0.0 0.0 1.191865966926858 6.48562343644885E-4 60-61 8.844031958793886E-4 0.0 0.0 1.3633370065546069 6.927825034388545E-4 62-63 8.844031958793886E-4 0.0 0.0 1.5334372212287426 7.370026632328239E-4 64-65 8.844031958793886E-4 0.0 0.0 1.7109959028547945 7.370026632328239E-4 66-67 8.844031958793886E-4 0.0 0.0 1.920496279905357 7.370026632328239E-4 68-69 8.844031958793886E-4 0.0 0.0 2.156278171926802 7.370026632328239E-4 70-71 9.138833024087016E-4 0.0 0.0 2.4010072762798935 7.664827697621368E-4 72-73 9.286233556733581E-4 0.0 0.0 2.6594888503289096 0.0010023236219966405 74-75 9.433634089380145E-4 0.0 0.0 2.92099213529718 0.0010760238883199228 76-77 9.433634089380145E-4 0.0 0.0 3.205519383464844 0.0011202440481138923 78-79 9.58103462202671E-4 0.0 0.0 3.529918475713404 0.0011202440481138923 80-81 0.0010023236219966405 0.0 0.0 3.867362515101185 0.0011497241546432053 82-83 0.0010318037285259533 0.0 0.0 4.217910461841245 0.001267644580760457 84-85 0.0010465437817906097 0.0 0.0 4.582048737691318 0.001267644580760457 86-87 0.0010612838350552664 0.0 0.0 4.9550015653936565 0.0012823846340251134 88-89 0.0010612838350552664 0.0 0.0 5.356962817920839 0.00129712468728977 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5575 0.0 46.075222 1 GTATCAA 9260 0.0 37.91089 1 TATCAAC 12150 0.0 28.54104 2 ATCAACG 12170 0.0 28.479422 3 TCAACGC 12295 0.0 28.15121 4 CAACGCA 12660 0.0 27.302029 5 AACGCAG 13145 0.0 26.304077 6 TAAGGTG 2180 0.0 23.77192 5 ACGCAGA 14820 0.0 23.331116 7 CGCAGAG 14925 0.0 23.10333 8 GTACATG 9535 0.0 21.65148 1 TACATGG 9110 0.0 21.303963 2 GTAAGGT 2335 0.0 20.768614 4 AGAGTAC 14165 0.0 20.704878 10-11 ACATGGG 9295 0.0 20.153065 3 GCAGAGT 16940 0.0 20.074833 9 GAGTACT 10360 0.0 19.415382 12-13 AGTACTT 11085 0.0 19.131018 12-13 AGGTAAG 2275 0.0 19.02736 2 GTGTAGC 2155 0.0 18.983213 1 >>END_MODULE