FastQCFastQC Report
Thu 26 May 2016
SRR938384_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938384_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3404898
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74740.21950730976375799No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58480.17175257526069798No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA48030.1410614943531348No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA47100.13833013499963875No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA41930.12314612655063381No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA40850.11997422536592874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39770.11680232418122363No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA38000.11160393057295696No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC36760.1079621181016289No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG34850.10235255211756711No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48750.044.5195161
GTATCAA90250.042.1763881
ATCAACG114350.032.7538453
TCAACGC117150.031.9304264
TATCAAC119050.031.620452
CAACGCA120300.031.0938845
AACGCAG125000.030.1064626
ACGCAGA139850.026.598737
CGCAGAG141550.026.2457188
TACATGG88900.023.417722
GCAGAGT156000.023.2654369
GTACATG93800.023.1378081
TAAGGTG21950.022.5198065
AGAGTAC131350.022.42221610-11
ACATGGG94850.021.2471543
GAGTACT94400.020.43027112-13
AGTACTT100750.020.22704112-13
GGTAAGG20850.019.60493
GTAAGGT22100.019.5713754
CAGAGTA146050.019.433610-11
Produced by FastQC (version 0.11.5)