FastQCFastQC Report
Thu 26 May 2016
SRR938384_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938384_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3404898
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78430.23034463881150039No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63490.18646667242307993No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA46620.13692040113976983No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA45890.1347764308945525No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45580.1338659777767205No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA43740.12846199798055624No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC41990.12332234328311745No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA40800.11982737808885904No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG39690.11656736853791214No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA34150.10029669023859158No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53750.045.263081
GTATCAA96500.040.3774721
ATCAACG122450.031.0321313
TATCAAC124850.030.5546652
TCAACGC124700.030.4722084
CAACGCA127700.029.9051195
AACGCAG132700.028.814126
ACGCAGA151550.025.073477
CGCAGAG152550.024.8156978
GCAGAGT170400.021.7701729
AGAGTAC143700.021.5346610-11
GTAAGGT21400.021.5297164
GGACCGA10050.021.2680076
GTACATG91950.021.2394241
TAAGGTG23700.020.8432485
AGGACCG12600.020.7334945
TACATGG90650.020.6480462
GAGTACT107100.020.37869612-13
GGTAAGG22300.019.8088133
ACATGGG92100.019.8039573
Produced by FastQC (version 0.11.5)