##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938384_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3404898 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.812300397838644 31.0 31.0 34.0 30.0 34.0 2 32.069441434075266 33.0 31.0 34.0 30.0 34.0 3 32.129785973030614 33.0 31.0 34.0 30.0 34.0 4 35.77799011894042 37.0 35.0 37.0 35.0 37.0 5 35.66788726123367 37.0 35.0 37.0 33.0 37.0 6 35.701420718036196 37.0 35.0 37.0 33.0 37.0 7 35.62580670551658 37.0 35.0 37.0 33.0 37.0 8 35.591526383462885 37.0 35.0 37.0 33.0 37.0 9 37.147042583948185 39.0 37.0 39.0 33.0 39.0 10-11 37.07186896641251 39.0 37.0 39.0 33.0 39.0 12-13 36.95093362561815 39.0 37.0 39.0 33.0 39.0 14-15 37.96573656538317 40.0 37.0 41.0 33.0 41.0 16-17 38.054390175564734 40.0 37.0 41.0 33.0 41.0 18-19 38.18684304199421 40.0 37.5 41.0 33.0 41.0 20-21 38.13702642487381 40.0 37.0 41.0 33.0 41.0 22-23 38.00512173932964 40.0 37.0 41.0 33.0 41.0 24-25 37.87945028015524 40.0 37.0 41.0 32.5 41.0 26-27 37.65184977641033 40.0 37.0 41.0 32.0 41.0 28-29 37.50828732608143 39.5 36.5 41.0 32.0 41.0 30-31 37.24451819114699 39.0 36.0 41.0 31.0 41.0 32-33 37.13288680013322 39.0 36.0 41.0 31.0 41.0 34-35 36.992547500688715 39.0 36.0 41.0 31.0 41.0 36-37 36.8500116596738 39.0 35.5 41.0 30.0 41.0 38-39 36.61707825021484 39.0 35.0 40.0 30.0 41.0 40-41 36.384486407522346 39.0 35.0 40.0 30.0 41.0 42-43 36.110250732914764 38.0 35.0 40.0 29.5 41.0 44-45 35.81161212465102 38.0 35.0 40.0 28.5 41.0 46-47 35.39947569648195 38.0 34.0 40.0 27.0 41.0 48-49 34.976150533731115 38.0 34.0 40.0 26.0 41.0 50-51 34.55960545660986 38.0 33.0 40.0 24.5 41.0 52-53 33.93250855091694 37.0 33.0 40.0 23.5 41.0 54-55 33.470350506828694 37.0 32.0 40.0 22.5 41.0 56-57 33.05428371128886 36.5 31.5 39.0 21.5 40.0 58-59 32.47457339397538 36.0 31.0 39.0 19.0 40.0 60-61 31.938103138478745 35.0 30.0 39.0 17.5 40.0 62-63 31.392008071901124 35.0 29.5 38.0 12.5 40.0 64-65 30.700513642405735 34.5 29.0 38.0 8.0 40.0 66-67 30.00709301717702 34.0 28.0 37.5 7.0 39.5 68-69 29.2324375062043 34.0 26.5 37.0 2.0 39.0 70-71 28.415205095717994 33.0 26.0 36.0 2.0 39.0 72-73 27.799526887442738 32.5 25.5 35.5 2.0 38.5 74-75 27.05899060706077 32.0 24.5 35.0 2.0 37.5 76-77 25.917692394896996 30.5 23.0 34.0 2.0 36.0 78-79 26.3818193672762 31.5 24.0 34.5 2.0 36.0 80-81 25.986117498967666 31.5 22.5 35.0 2.0 36.0 82-83 25.271442639397712 31.0 20.0 34.0 2.0 36.0 84-85 24.44532919928879 31.0 17.0 34.0 2.0 35.0 86-87 23.498402742167315 30.0 8.5 34.0 2.0 35.0 88-89 22.47172837482944 29.0 2.0 34.0 2.0 35.0 90-91 21.55477359380516 29.0 2.0 34.0 2.0 35.0 92-93 20.428504466213084 27.0 2.0 33.0 2.0 34.5 94-95 19.214816273497767 25.5 2.0 32.5 2.0 34.0 96-97 17.79895756054954 24.0 2.0 32.0 2.0 34.0 98-99 16.01179168950142 10.0 2.0 32.0 2.0 34.0 100-101 11.92278035935291 2.0 2.0 25.5 2.0 31.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2930546817639197 1101 2 0.27268687037079076 1101 3 0.2142713491011108 1101 4 0.14133601007573304 1101 5 0.09159003275588873 1101 6 0.20750330084886315 1101 7 0.24417184494732425 1101 8 0.28937254015122704 1101 9 0.30615546779331027 1101 10-11 0.36525568641431505 1101 12-13 0.32817375258596826 1101 14-15 0.44160205447800394 1101 16-17 0.39459700047175517 1101 18-19 0.4180451926154589 1101 20-21 0.47299810055110214 1101 22-23 0.4575239100034736 1101 24-25 0.47159233549605517 1101 26-27 0.6227065041458246 1101 28-29 0.5896358025731132 1101 30-31 0.6207761283692506 1101 32-33 0.5285045014825229 1101 34-35 0.27948583203398414 1101 36-37 0.3622126802831076 1101 38-39 0.5412850667216418 1101 40-41 0.5314586798343512 1101 42-43 0.6087807231672784 1101 44-45 0.7387472232771799 1101 46-47 0.4659680730353344 1101 48-49 0.4726472749209094 1101 50-51 0.5762495370394092 1101 52-53 0.7427471341388312 1101 54-55 0.8016392564415327 1101 56-57 0.8781283992264619 1101 58-59 0.9386739982496337 1101 60-61 0.9906078818752668 1101 62-63 1.0022681047556539 1101 64-65 1.0973692711203 1101 66-67 1.1738750039884636 1101 68-69 1.4731900334654888 1101 70-71 1.6644824697484175 1101 72-73 1.8440313916261317 1101 74-75 2.0165893335047365 1101 76-77 1.933061167374948 1101 78-79 2.05413233230475 1101 80-81 2.1788247519437896 1101 82-83 2.347832113337706 1101 84-85 2.5693248606557475 1101 86-87 2.59075135931457 1101 88-89 2.541826153707113 1101 90-91 2.9137241082350265 1101 92-93 3.236435741678431 1101 94-95 3.08758960012781 1101 96-97 3.251880641837939 1101 98-99 3.010001083765797 1101 100-101 2.5641226280429095 1102 1 0.2153100912116841 1102 2 0.20441137820193234 1102 3 0.20595019474961163 1102 4 0.2117958896298049 1102 5 0.17483127378813634 1102 6 0.24830417989537068 1102 7 0.23975060456302089 1102 8 0.22241246801326042 1102 9 0.30773060611174685 1102 10-11 0.23571682586305798 1102 12-13 0.31114906584977575 1102 14-15 0.38720884675016976 1102 16-17 0.3144605898756865 1102 18-19 0.3218797270483762 1102 20-21 0.2479266915208953 1102 22-23 0.3795421442790854 1102 24-25 0.3199061914577044 1102 26-27 0.3943064533900653 1102 28-29 0.2547557985414528 1102 30-31 0.27380001057269254 1102 32-33 0.29275139044260357 1102 34-35 0.48604254889245624 1102 36-37 0.40716315742321996 1102 38-39 0.470434304845341 1102 40-41 0.39324431955487427 1102 42-43 0.4132895712275868 1102 44-45 0.3911958285737782 1102 46-47 0.349729380590027 1102 48-49 0.49252058351230943 1102 50-51 0.609591299668061 1102 52-53 0.699822252658926 1102 54-55 0.5804705511452966 1102 56-57 0.8592227347400083 1102 58-59 0.9779105380749016 1102 60-61 0.9587819525229584 1102 62-63 1.1374978195514949 1102 64-65 1.127093246195983 1102 66-67 1.0789751926126918 1102 68-69 1.3976572780038445 1102 70-71 1.5255166272928484 1102 72-73 1.6650734775077503 1102 74-75 1.7458779249784904 1102 76-77 1.572459018714575 1102 78-79 1.5664292642812363 1102 80-81 1.7537595073982324 1102 82-83 1.8059940260018088 1102 84-85 2.0297516139791725 1102 86-87 2.02513865453669 1102 88-89 1.790791330218358 1102 90-91 1.9883948257630415 1102 92-93 2.1802455641772696 1102 94-95 2.1294406913765833 1102 96-97 2.2417976147789993 1102 98-99 2.1116395317915426 1102 100-101 1.673219427550471 1103 1 0.2374279260005352 1103 2 0.24176466344616898 1103 3 0.2094239018396351 1103 4 0.19289385143890314 1103 5 0.16734400585456655 1103 6 0.20246812225913402 1103 7 0.22288905452008123 1103 8 0.2593328983411993 1103 9 0.26066339917900194 1103 10-11 0.29459066089223995 1103 12-13 0.3190297820263055 1103 14-15 0.4452503262498624 1103 16-17 0.35486859914950486 1103 18-19 0.30722958939009004 1103 20-21 0.348520398116122 1103 22-23 0.4087018013880197 1103 24-25 0.3133084452366788 1103 26-27 0.45108418031419006 1103 28-29 0.4168002803701967 1103 30-31 0.3517666880776673 1103 32-33 0.3677907087601682 1103 34-35 0.5249565946324708 1103 36-37 0.6073958423430099 1103 38-39 0.5659791860421706 1103 40-41 0.6600729570735595 1103 42-43 0.6838586619053402 1103 44-45 0.8193886526715701 1103 46-47 0.877521069492893 1103 48-49 0.878629024315714 1103 50-51 0.9128632021798353 1103 52-53 1.0416743486345013 1103 54-55 1.1644790845455404 1103 56-57 1.3385875539464323 1103 58-59 1.2433908223684327 1103 60-61 1.1839528325817703 1103 62-63 1.3672205238035282 1103 64-65 1.511274912727174 1103 66-67 1.4494600802418276 1103 68-69 1.53351143090174 1103 70-71 1.754508099193945 1103 72-73 1.7213952003038955 1103 74-75 1.6244816917616696 1103 76-77 1.651142349868465 1103 78-79 1.8514748735296074 1103 80-81 2.0913540038940432 1103 82-83 2.159077421478699 1103 84-85 2.2910555419232708 1103 86-87 2.3589164468440806 1103 88-89 2.3840086995490104 1103 90-91 2.6050290364102686 1103 92-93 2.691284954946564 1103 94-95 2.4910952591931697 1103 96-97 2.464639790168736 1103 98-99 2.476850728588115 1103 100-101 2.2494655089720794 1104 1 0.2455759823624959 1104 2 0.27241788699573277 1104 3 0.2679568420283829 1104 4 0.18306371957025647 1104 5 0.16478072677249145 1104 6 0.15276685819477365 1104 7 0.18156268045278523 1104 8 0.1923300694535044 1104 9 0.20953437290305033 1104 10-11 0.34161455470948 1104 12-13 0.28023229531937943 1104 14-15 0.42758933421728074 1104 16-17 0.3907606175726741 1104 18-19 0.4467949050015676 1104 20-21 0.3788573105487316 1104 22-23 0.40376835948473655 1104 24-25 0.3937723457299569 1104 26-27 0.5047395651474389 1104 28-29 0.5016208449339103 1104 30-31 0.5207896934421044 1104 32-33 0.774573506493013 1104 34-35 0.5847148242005815 1104 36-37 0.6361342140635742 1104 38-39 0.7004630404775156 1104 40-41 0.6606778467671646 1104 42-43 0.6320733269400876 1104 44-45 0.755108375312453 1104 46-47 0.7778279818917824 1104 48-49 0.941322688483929 1104 50-51 0.8075140250523489 1104 52-53 0.9256271965725134 1104 54-55 0.9128275654092519 1104 56-57 0.9454175138564764 1104 58-59 1.0237230986620816 1104 60-61 1.0902093863963778 1104 62-63 1.1758096877138051 1104 64-65 1.327430803321036 1104 66-67 1.4471876978058837 1104 68-69 1.6916366437426653 1104 70-71 1.8618723951920337 1104 72-73 1.9454432323893442 1104 74-75 1.70290084424175 1104 76-77 2.01225960035147 1104 78-79 2.0987222292445367 1104 80-81 2.2249061629861018 1104 82-83 2.2677185101452793 1104 84-85 2.4021948351396745 1104 86-87 2.2549163861528108 1104 88-89 2.478362303767728 1104 90-91 2.708795393551437 1104 92-93 2.8388818333920582 1104 94-95 2.5919822368688976 1104 96-97 2.6101901504388785 1104 98-99 2.7634017530905304 1104 100-101 2.4462875621578686 1105 1 0.2173781399429231 1105 2 0.15858786066811348 1105 3 0.17435734289527005 1105 4 0.14773012938211139 1105 5 0.08887852424246745 1105 6 0.04295821503032471 1105 7 0.1167261043274408 1105 8 0.08393733844470574 1105 9 0.17444158410192045 1105 10-11 0.201160724362083 1105 12-13 0.2587512062549706 1105 14-15 0.36347676759555725 1105 16-17 0.254764823570234 1105 18-19 0.2723567749046083 1105 20-21 0.2470643034210056 1105 22-23 0.3038261518176171 1105 24-25 0.283425912801178 1105 26-27 0.2480597346716138 1105 28-29 0.30729332294072265 1105 30-31 0.4478332945465766 1105 32-33 0.4543132804607808 1105 34-35 0.37970294466548893 1105 36-37 0.39950430974199236 1105 38-39 0.3725991093146064 1105 40-41 0.6192985364223418 1105 42-43 0.6007643306002635 1105 44-45 0.5101868765646245 1105 46-47 0.6316840147691991 1105 48-49 0.6799440202761318 1105 50-51 0.7511424203519041 1105 52-53 0.7315581347347191 1105 54-55 0.8765540471404449 1105 56-57 0.9257713313611333 1105 58-59 0.9754335911811864 1105 60-61 1.0298460973704984 1105 62-63 1.1864932425844081 1105 64-65 1.2810307840569237 1105 66-67 1.3481942476036082 1105 68-69 1.4599696560074804 1105 70-71 1.4899626796587349 1105 72-73 1.6194005623835679 1105 74-75 1.7162067262169636 1105 76-77 1.6401468089817897 1105 78-79 1.6278969226241031 1105 80-81 1.8237551323561654 1105 82-83 2.053721528189328 1105 84-85 2.4027904172368935 1105 86-87 2.3410654903716512 1105 88-89 2.159775647375252 1105 90-91 2.4552740746019737 1105 92-93 2.5897150061911347 1105 94-95 2.6746180488511726 1105 96-97 2.620030097783644 1105 98-99 2.6652262249328196 1105 100-101 2.5329471653171804 1106 1 0.08836786569631272 1106 2 0.1171372606703116 1106 3 0.08950420861467734 1106 4 0.07377971372144998 1106 5 0.08053158133678551 1106 6 0.017989238579986022 1106 7 0.09481932087730627 1106 8 0.07600958605577546 1106 9 0.009910090688642015 1106 10-11 0.1703486133330756 1106 12-13 0.13250669518768632 1106 14-15 0.0961912379831702 1106 16-17 0.11372309905308242 1106 18-19 0.18281830021213352 1106 20-21 0.1644053695015586 1106 22-23 0.22658571028701147 1106 24-25 0.23260902469461087 1106 26-27 0.052175630181480415 1106 28-29 0.27023473196680925 1106 30-31 0.2943030420752848 1106 32-33 0.18967302813068443 1106 34-35 0.12317917458646832 1106 36-37 0.15820823918244287 1106 38-39 0.21317861824044826 1106 40-41 0.31868073243469297 1106 42-43 0.34260552924668275 1106 44-45 0.36948260668876287 1106 46-47 -0.049474257889762896 1106 48-49 0.11475687492903575 1106 50-51 0.5176189452123197 1106 52-53 0.3880586861773381 1106 54-55 0.3334493154019853 1106 56-57 0.4467794908292362 1106 58-59 0.7516970066112236 1106 60-61 0.7951851051503134 1106 62-63 0.5561958636233157 1106 64-65 0.8209197738736727 1106 66-67 0.7774499255799476 1106 68-69 0.7804072331741487 1106 70-71 0.717562147198926 1106 72-73 1.0809968593248271 1106 74-75 0.9964123288111715 1106 76-77 0.9795952722375141 1106 78-79 1.1552824021909167 1106 80-81 1.440271858402923 1106 82-83 1.7326712065013936 1106 84-85 1.6893858121027527 1106 86-87 1.7486555527952667 1106 88-89 1.765626390763824 1106 90-91 1.905475786505761 1106 92-93 1.9277209332187262 1106 94-95 1.7102937582354265 1106 96-97 1.7816037643985396 1106 98-99 1.9229512064598886 1106 100-101 1.807967010976002 1107 1 0.05504756040757286 1107 2 0.05753801606926601 1107 3 0.12405235209556054 1107 4 0.120309788510923 1107 5 0.15861130067764861 1107 6 0.03331156962722304 1107 7 0.03456078286064468 1107 8 0.1208678675633621 1107 9 0.06558756759724815 1107 10-11 0.0729216028201165 1107 12-13 0.09417066860774526 1107 14-15 0.17880770638758747 1107 16-17 0.10639381860253394 1107 18-19 0.1292578615805624 1107 20-21 0.22165387531687486 1107 22-23 0.24414724124082454 1107 24-25 0.24482370805204567 1107 26-27 0.2584657248992315 1107 28-29 0.287993314940465 1107 30-31 0.27380875942923666 1107 32-33 0.3188656285352067 1107 34-35 0.34992747253731693 1107 36-37 0.32595224243117826 1107 38-39 0.44035121919280584 1107 40-41 0.36666411329346005 1107 42-43 0.4815962890472534 1107 44-45 0.37889656396603044 1107 46-47 0.5607523701434829 1107 48-49 0.5407199467583297 1107 50-51 0.6207129274925123 1107 52-53 0.5098596733495313 1107 54-55 0.6659567678304583 1107 56-57 0.572054458826301 1107 58-59 0.8120473555420133 1107 60-61 0.9006059629662424 1107 62-63 1.0387119166857808 1107 64-65 1.1617613430633256 1107 66-67 1.2808566036933833 1107 68-69 1.331192743833661 1107 70-71 1.2163981213859465 1107 72-73 1.5715390452637799 1107 74-75 1.3506402327884892 1107 76-77 1.492954822518822 1107 78-79 1.8074588785502534 1107 80-81 1.9358892150968998 1107 82-83 2.001018612323314 1107 84-85 2.131611783650019 1107 86-87 2.527838429589348 1107 88-89 2.410972704187621 1107 90-91 2.4967797298755734 1107 92-93 2.4555075822576953 1107 94-95 2.3053510607655134 1107 96-97 2.167612926824262 1107 98-99 2.2287894473142096 1107 100-101 1.9640066781470207 1108 1 0.05889905685009822 1108 2 0.10580686021098273 1108 3 0.03101591808042059 1108 4 0.025574606983688852 1108 5 -0.005483287183267294 1108 6 0.06316212639918461 1108 7 0.06661363323726022 1108 8 0.04271015891188057 1108 9 0.024425064203363434 1108 10-11 0.08215759840502557 1108 12-13 0.04162248531754642 1108 14-15 0.19236890095564974 1108 16-17 0.15113900757022947 1108 18-19 -0.008772032026804766 1108 20-21 0.1046671152736991 1108 22-23 0.22078601525312536 1108 24-25 0.18534682246675516 1108 26-27 0.2709405485066938 1108 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-0.3102059086134936 2316 64-65 -0.20726010390972505 2316 66-67 0.03528632297761192 2316 68-69 0.4222873892938779 2316 70-71 0.1805718297358503 2316 72-73 0.41641460068937164 2316 74-75 0.45859304088149244 2316 76-77 0.47854622427601257 2316 78-79 0.28216749269644836 2316 80-81 0.012279373809342076 2316 82-83 0.6534349765800442 2316 84-85 0.6479416530277078 2316 86-87 0.6928011383232082 2316 88-89 0.837982610172876 2316 90-91 0.6801353964095718 2316 92-93 0.8312299056119521 2316 94-95 1.1110733253314429 2316 96-97 0.9802703812333426 2316 98-99 0.929292866236981 2316 100-101 0.4579285380813207 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 21.0 4 47.0 5 104.0 6 252.0 7 679.0 8 1668.0 9 3108.0 10 5104.0 11 7725.0 12 11353.0 13 15711.0 14 21524.0 15 27859.0 16 35218.0 17 43435.0 18 44626.0 19 43325.0 20 45666.0 21 49342.0 22 54473.0 23 60390.0 24 67957.0 25 76629.0 26 87470.0 27 102103.0 28 119717.0 29 139007.0 30 160612.0 31 186221.0 32 216905.0 33 256038.0 34 307132.0 35 366180.0 36 408088.0 37 337682.0 38 99766.0 39 1752.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.95669250397019 19.18321376260898 15.296455297430587 23.56363843599025 2 16.05140618588436 20.26827489438222 39.23905850298414 24.441260416749277 3 19.561733844842585 23.232758443456436 29.90164548790452 27.30386222379646 4 13.55570710194549 16.854220008940064 36.17239047983229 33.41768240928216 5 14.099868130835358 36.93808023166681 34.25179668065635 14.710254956841485 6 33.285431751553205 36.05218129882305 16.644551466739973 14.017835482883775 7 29.256588596780286 32.012177750992834 20.083773434622714 18.64746021760417 8 26.541897314925333 35.37056638440645 19.18828064058344 18.899255660084773 9 25.99978384004633 16.793131987385188 19.6796897164839 37.52739445608458 10-11 25.18975605142944 26.29915786023546 27.271609898446297 21.2394761898888 12-13 26.16313322748582 23.28742300063027 27.48539016440434 23.06405360747958 14-15 22.512583343172103 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22.94671729432806 40-41 23.050454656604472 26.157173205698058 28.193776311741033 22.598595825956437 42-43 22.67087376616175 26.30824963337671 27.975845891631064 23.045030708830474 44-45 22.61914883962513 26.460602968777515 28.257411921367325 22.662836270230034 46-47 23.056646835900494 26.775616689445148 27.48179128604425 22.685945188610102 48-49 22.801138242614023 26.829188422090766 27.410659585103573 22.959013750191637 50-51 23.000586214330063 26.89958700671797 27.46944255011457 22.6303842288374 52-53 23.38432751876855 26.5512946349641 27.38720807495555 22.677169771311796 54-55 22.712442487263935 26.758966054196044 27.684426963744578 22.84416449479544 56-57 22.854972454387767 26.920923916076195 27.408589038496896 22.815514591039143 58-59 22.880465141687065 26.531734577658423 27.420013756652917 23.1677865240016 60-61 22.441362413793307 26.836589524855075 27.69708519902799 23.024962862323626 62-63 22.648108695179708 26.79105218423577 27.79732315035575 22.76351597022877 64-65 22.338897381563896 26.849139253927067 27.5711585819827 23.240804782526332 66-67 22.262239977796558 26.871877182071895 27.57278345549877 23.293099384632782 68-69 22.83835504270138 26.478497761953367 27.634416797251816 23.048730398093433 70-71 22.6434536364966 26.55370293030804 27.268922593275924 23.533920839919436 72-73 22.584307826820176 26.70887754474192 27.30794268299828 23.398871945439623 74-75 22.641794262265712 27.007461016453355 27.078916901475463 23.27182781980547 76-77 22.659445304969488 26.620489071919334 27.453950162383716 23.26611546072746 78-79 22.687316918157315 26.878485053003054 27.399132073853604 23.035065954986024 80-81 22.915672080689774 26.85009879166523 27.131076825479806 23.103152302165192 82-83 22.836437573479547 26.96373036299007 27.208160455396246 22.99167160813414 84-85 22.69149975416177 27.012525119849805 27.15443281273589 23.14154231325253 86-87 22.89365282862593 26.982652151629566 26.84960253998464 23.274092479759865 88-89 22.63578820863356 27.210066204626393 26.591413311059537 23.562732275680506 90-91 22.563079137113235 27.085143251538245 26.894474059062805 23.457303552285712 92-93 22.69121751463121 26.728791243389267 27.105456610939772 23.474534631039752 94-95 22.431052225971072 26.535259814102986 27.179185502288473 23.854502457637473 96-97 22.593634991014415 26.30013477647048 27.153141490036266 23.953088742478847 98-99 22.342915999245793 26.546801108285766 26.818821004329646 24.291461888138794 100-101 22.55887623032356 26.086736250758836 27.171497405061007 24.1828901138566 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 280.0 1 201.5 2 172.5 3 251.0 4 232.5 5 219.5 6 383.5 7 918.5 8 1533.0 9 1734.0 10 1522.0 11 1327.0 12 1432.0 13 1613.5 14 1850.0 15 2112.0 16 2442.0 17 2751.0 18 3148.0 19 3688.0 20 4268.5 21 5222.5 22 6424.5 23 7996.5 24 10446.0 25 13562.5 26 17376.5 27 22033.5 28 26690.5 29 31672.0 30 37651.0 31 46124.5 32 57682.0 33 62269.5 34 62057.5 35 65604.0 36 71776.5 37 78734.0 38 84355.0 39 89352.0 40 95584.0 41 102633.0 42 107196.5 43 110873.5 44 114776.5 45 120778.5 46 125317.0 47 129554.5 48 132002.0 49 129655.5 50 126697.0 51 122563.0 52 119086.0 53 119479.0 54 121733.5 55 116203.0 56 106990.0 57 98663.5 58 91117.0 59 80750.5 60 64950.0 61 53484.5 62 46038.5 63 35679.0 64 25700.5 65 19302.0 66 14427.5 67 11215.5 68 8699.5 69 6602.0 70 5062.5 71 3576.0 72 2528.5 73 1967.0 74 1537.0 75 1125.0 76 800.5 77 560.5 78 369.5 79 237.5 80 154.5 81 98.0 82 63.5 83 40.0 84 24.0 85 14.0 86 6.5 87 5.5 88 3.0 89 1.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02279069740121437 2 0.011894629442644097 3 0.002114600789803395 4 0.0 5 2.3495564331148832E-4 6 0.0 7 0.0 8 5.873891082787208E-5 9 3.524334649672325E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0016153200477664823 26-27 0.0090457922674923 28-29 2.055861878975523E-4 30-31 0.0 32-33 3.3774873726026447E-4 34-35 0.00979471338054767 36-37 0.001321625493627122 38-39 0.012775713105062179 40-41 0.011909314170351066 42-43 0.005198393608266679 44-45 8.370294792971771E-4 46-47 1.0279309394877614E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 8.810836624180813E-5 66-67 6.020738359856888E-4 68-69 9.251378455389853E-4 70-71 0.0 72-73 2.790098264323924E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.6153200477664822E-4 82-83 0.0011454087611435057 84-85 0.006461280191065929 86-87 0.0044935266783322145 88-89 0.0 90-91 0.00140973385986893 92-93 5.580196528647848E-4 94-95 5.873891082787208E-5 96-97 2.936945541393604E-5 98-99 0.0 100-101 2.936945541393604E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3404898.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.99347698875716 #Duplication Level Percentage of deduplicated Percentage of total 1 73.21842797804031 37.33662222251172 2 14.51298879812152 14.801355206302006 3 5.1514500650084205 7.880710510462154 4 2.4001097823006585 4.895597718169584 5 1.2861152298733394 3.279174368971813 6 0.7862461544549414 2.405605511081812 7 0.5253493517516451 1.8752573055724209 8 0.3594626468991032 1.4664200170373727 9 0.27168046511683885 1.2468538391607327 >10 1.347770334086146 12.301258922649478 >50 0.08940421552002821 3.1553730298787954 >100 0.04384252149940686 4.172241638525891 >500 0.0037492719910669 1.2580491836011005 >1k 0.0032878231216436657 3.508042683262714 >5k 1.1536221479451458E-4 0.41743784281240615 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7843 0.23034463881150039 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6349 0.18646667242307993 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4662 0.13692040113976983 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4589 0.1347764308945525 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4558 0.1338659777767205 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4374 0.12846199798055624 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4199 0.12332234328311745 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4080 0.11982737808885904 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3969 0.11656736853791214 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3415 0.10029669023859158 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.873891082787208E-5 2 2.936945541393604E-5 0.0 0.0 0.0 5.873891082787208E-5 3 2.936945541393604E-5 0.0 0.0 0.0 5.873891082787208E-5 4 2.936945541393604E-5 0.0 0.0 0.0 5.873891082787208E-5 5 2.936945541393604E-5 0.0 0.0 0.0 5.873891082787208E-5 6 2.936945541393604E-5 0.0 0.0 0.0 5.873891082787208E-5 7 5.873891082787208E-5 0.0 0.0 0.0 5.873891082787208E-5 8 5.873891082787208E-5 0.0 0.0 0.0 5.873891082787208E-5 9 5.873891082787208E-5 0.0 0.0 0.0 5.873891082787208E-5 10-11 5.873891082787208E-5 0.0 0.0 0.0 5.873891082787208E-5 12-13 7.34236385348401E-5 0.0 0.0 0.0 1.6153200477664822E-4 14-15 1.1747782165574418E-4 0.0 0.0 1.468472770696802E-5 2.2027091560452032E-4 16-17 1.6153200477664825E-4 2.936945541393604E-5 0.0 4.405418312090406E-5 2.936945541393604E-4 18-19 1.7621673248361625E-4 2.936945541393604E-5 0.0 5.873891082787208E-5 3.2306400955329644E-4 20-21 1.7621673248361625E-4 2.936945541393604E-5 0.0 5.873891082787208E-5 3.2306400955329644E-4 22-23 1.7621673248361625E-4 2.936945541393604E-5 0.0 1.3216254936271218E-4 3.3774873726026447E-4 24-25 2.2027091560452032E-4 2.936945541393604E-5 0.0 2.4964037101845633E-4 3.524334649672325E-4 26-27 2.3495564331148832E-4 2.936945541393604E-5 0.0 6.46128019106593E-4 3.964876480881366E-4 28-29 3.230640095532965E-4 2.936945541393604E-5 0.0 0.0024817189824775953 4.111723757951046E-4 30-31 4.111723757951046E-4 2.936945541393604E-5 0.0 0.009471649370994373 4.111723757951046E-4 32-33 4.4054183120904064E-4 2.936945541393604E-5 0.0 0.023789258885288194 4.258571035020726E-4 34-35 5.139654697438806E-4 2.936945541393604E-5 0.0 0.045287700248289375 5.139654697438806E-4 36-37 5.286501974508487E-4 2.936945541393604E-5 0.0 0.08130933731348193 5.286501974508487E-4 38-39 5.580196528647848E-4 2.936945541393604E-5 0.0 0.1371553567830813 5.873891082787209E-4 40-41 5.873891082787209E-4 2.936945541393604E-5 0.0 0.19701030691668298 5.873891082787209E-4 42-43 6.608127468135609E-4 2.936945541393604E-5 0.0 0.2616524782827562 5.873891082787209E-4 44-45 6.90182202227497E-4 2.936945541393604E-5 0.0 0.34161082064719706 6.314432913996249E-4 46-47 7.04866929934465E-4 2.936945541393604E-5 0.0 0.43838317623611633 6.90182202227497E-4 48-49 7.04866929934465E-4 2.936945541393604E-5 0.0 0.5418958218425339 7.04866929934465E-4 50-51 7.04866929934465E-4 2.936945541393604E-5 0.0 0.6542780429839602 7.04866929934465E-4 52-53 7.19551657641433E-4 2.936945541393604E-5 0.0 0.7632827767527838 7.636058407623371E-4 54-55 7.929752961762732E-4 2.936945541393604E-5 0.0 0.8840499774148889 7.636058407623371E-4 56-57 8.223447515902091E-4 4.405418312090406E-5 0.0 1.0255960677823537 7.782905684693051E-4 58-59 8.223447515902091E-4 5.873891082787208E-5 0.0 1.1795066988790852 7.929752961762731E-4 60-61 8.223447515902091E-4 5.873891082787208E-5 0.0 1.347367821297437 8.223447515902091E-4 62-63 8.223447515902091E-4 5.873891082787208E-5 0.0 1.5173729139610055 9.398225732459533E-4 64-65 8.223447515902091E-4 5.873891082787208E-5 0.0 1.6935309075337939 9.691920286598894E-4 66-67 8.810836624180813E-4 5.873891082787208E-5 0.0 1.9012757504042705 9.691920286598894E-4 68-69 8.810836624180813E-4 5.873891082787208E-5 0.0 2.1386837432428223 9.691920286598894E-4 70-71 9.251378455389853E-4 5.873891082787208E-5 0.0 2.3839921195877234 9.985614840738255E-4 72-73 9.398225732459533E-4 5.873891082787208E-5 0.0 2.641253864286096 0.0011894629442644098 74-75 9.398225732459533E-4 5.873891082787208E-5 0.0 2.9000134512105795 0.0012628865827992498 76-77 9.398225732459533E-4 5.873891082787208E-5 0.0 3.1829440999407326 0.0012628865827992498 78-79 9.398225732459533E-4 5.873891082787208E-5 0.0 3.496110603019533 0.0012628865827992498 80-81 0.0010279309394877615 5.873891082787208E-5 0.0 3.8339033944629177 0.0012628865827992498 82-83 0.0011013545780226017 5.873891082787208E-5 0.0 4.182974056785255 0.001321625493627122 84-85 0.0011454087611435055 5.873891082787208E-5 0.0 4.53912275786235 0.001321625493627122 86-87 0.0011747782165574417 5.873891082787208E-5 0.0 4.908311497143233 0.0013950491321619621 88-89 0.0011747782165574417 5.873891082787208E-5 0.0 5.3025083277090825 0.0014684727706968021 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5375 0.0 45.26308 1 GTATCAA 9650 0.0 40.377472 1 ATCAACG 12245 0.0 31.032131 3 TATCAAC 12485 0.0 30.554665 2 TCAACGC 12470 0.0 30.472208 4 CAACGCA 12770 0.0 29.905119 5 AACGCAG 13270 0.0 28.81412 6 ACGCAGA 15155 0.0 25.07347 7 CGCAGAG 15255 0.0 24.815697 8 GCAGAGT 17040 0.0 21.770172 9 AGAGTAC 14370 0.0 21.53466 10-11 GTAAGGT 2140 0.0 21.529716 4 GGACCGA 1005 0.0 21.268007 6 GTACATG 9195 0.0 21.239424 1 TAAGGTG 2370 0.0 20.843248 5 AGGACCG 1260 0.0 20.733494 5 TACATGG 9065 0.0 20.648046 2 GAGTACT 10710 0.0 20.378696 12-13 GGTAAGG 2230 0.0 19.808813 3 ACATGGG 9210 0.0 19.803957 3 >>END_MODULE