FastQCFastQC Report
Thu 26 May 2016
SRR938383_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938383_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3222705
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77560.24066738966179035No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA67650.20991682453094526No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA62640.19437087788053825No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA59440.18444133111780323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58850.18261057093342392No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA58390.18118319858628076No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC49610.15393900465602653No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA49500.15359767648605752No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG49490.15356664665242398No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA45210.14028587785726587No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA43980.13646920832033957No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42440.13169061394077336No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG39900.12380903619785243No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT36930.11459317560868897No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA35370.10975252156185565No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT33570.10416715150781718No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG32550.1010021084771954No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39500.054.784271
GTATCAA81050.047.3545951
ATCAACG106600.035.690623
TCAACGC106600.035.6460084
TATCAAC107400.035.5133322
CAACGCA109050.034.8015485
AACGCAG114850.033.22326
ACGCAGA131800.028.8468887
CGCAGAG133000.028.5151528
TAAGGTA9750.026.827454
TACATGG108850.026.1272742
GTACATG113250.025.7852751
TAAGGTG21650.025.2616255
GTGTAAG12000.024.9689851
GCAGAGT150400.024.5522239
ACATGGG112150.023.4502333
GTAAGGT22400.023.3542564
AGGTAAG21400.023.1121792
AAGGTAA20600.023.0873641
TGGTATC18300.022.3495452
Produced by FastQC (version 0.11.5)