##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938383_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3222705 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.08672279963571 31.0 31.0 34.0 28.0 34.0 2 31.122040645979077 31.0 31.0 34.0 27.0 34.0 3 31.169308081254723 31.0 31.0 34.0 27.0 34.0 4 34.838507713240894 37.0 35.0 37.0 32.0 37.0 5 34.800888384136925 37.0 35.0 37.0 32.0 37.0 6 34.82151515574649 37.0 35.0 37.0 32.0 37.0 7 34.71977174454379 37.0 35.0 37.0 31.0 37.0 8 34.682570077000534 37.0 35.0 37.0 30.0 37.0 9 36.16997211969448 39.0 35.0 39.0 31.0 39.0 10-11 36.001150586231134 38.5 35.0 39.0 30.0 39.0 12-13 35.85324533272515 38.0 35.0 39.0 30.0 39.0 14-15 36.81081855149634 39.0 36.0 41.0 30.5 41.0 16-17 36.614590382923666 39.0 36.0 40.5 30.0 41.0 18-19 36.755074696567014 39.0 36.0 41.0 30.0 41.0 20-21 36.71751029026858 39.0 36.0 41.0 30.0 41.0 22-23 36.62573536826982 39.0 36.0 41.0 30.0 41.0 24-25 36.465838170108654 39.0 36.0 41.0 29.5 41.0 26-27 36.23629869938452 39.0 35.0 40.5 29.0 41.0 28-29 36.017403857939215 39.0 35.0 40.0 27.5 41.0 30-31 35.74651806479339 38.0 35.0 40.0 27.0 41.0 32-33 35.45908483711665 38.0 34.0 40.0 26.5 41.0 34-35 35.19561889778928 38.0 34.0 40.0 25.5 41.0 36-37 34.89355293146596 38.0 34.0 40.0 25.0 41.0 38-39 34.726918070378765 38.0 33.0 40.0 24.5 41.0 40-41 34.609321672321855 38.0 33.0 40.0 24.0 41.0 42-43 34.27684305575596 38.0 33.0 40.0 23.5 41.0 44-45 33.91398685265949 38.0 33.0 40.0 22.5 41.0 46-47 33.510028376782856 37.5 32.5 40.0 20.5 41.0 48-49 33.11096796014528 37.0 31.5 40.0 18.5 41.0 50-51 32.38867224893374 36.5 30.5 39.5 16.0 40.5 52-53 32.386516451242045 36.5 31.0 39.0 15.0 40.0 54-55 32.443371329364616 36.5 31.0 39.0 14.0 41.0 56-57 32.074312727972305 36.0 31.0 39.0 10.5 41.0 58-59 31.65422587546797 36.0 30.5 39.0 8.0 40.0 60-61 31.13392445166405 35.0 29.5 39.0 6.5 40.0 62-63 30.522977591805642 35.0 28.5 38.0 2.0 40.0 64-65 29.95433913436073 34.5 28.0 38.0 2.0 40.0 66-67 29.377812272609503 34.0 27.5 37.0 2.0 39.5 68-69 28.745055628734246 34.0 26.0 36.5 2.0 39.0 70-71 28.06139438763399 33.5 26.0 36.0 2.0 39.0 72-73 27.40610760215409 33.0 25.0 35.5 2.0 38.5 74-75 26.715869898113542 32.0 23.5 35.0 2.0 37.5 76-77 25.945562966514153 32.0 21.0 35.0 2.0 37.0 78-79 25.140109783551395 31.0 18.5 35.0 2.0 36.0 80-81 24.43505998842587 31.0 15.0 34.0 2.0 36.0 82-83 23.678882181273185 30.0 7.5 34.0 2.0 35.0 84-85 22.958637697213987 30.0 3.5 34.0 2.0 35.0 86-87 22.174308694093938 29.0 2.0 34.0 2.0 35.0 88-89 21.301058272476073 29.0 2.0 34.0 2.0 35.0 90-91 20.353083046695247 27.0 2.0 33.0 2.0 35.0 92-93 19.374532884641937 25.5 2.0 33.0 2.0 35.0 94-95 18.32652538783413 24.5 2.0 32.5 2.0 34.0 96-97 17.042259064978023 19.5 2.0 32.0 2.0 34.0 98-99 15.500455828256076 2.0 2.0 31.5 2.0 34.0 100-101 12.14099087567742 2.0 2.0 27.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.49250029507241067 1101 2 0.431427244868388 1101 3 0.41242601803975987 1101 4 0.32096387136954974 1101 5 0.34148102855972695 1101 6 0.5472684463023754 1101 7 0.4641718093257978 1101 8 0.5183054956098587 1101 9 0.735372750853962 1101 10-11 0.7293321750502386 1101 12-13 0.7902911120287328 1101 14-15 0.8931022710435386 1101 16-17 0.8354136110901038 1101 18-19 0.8693562390495586 1101 20-21 0.906874826634116 1101 22-23 0.8859572452161615 1101 24-25 0.9003619908118878 1101 26-27 0.8425279870824127 1101 28-29 0.8490444994854371 1101 30-31 0.7946359197272557 1101 32-33 0.9523904145981206 1101 34-35 0.8352360451668517 1101 36-37 0.9375230100492757 1101 38-39 0.9083137434150146 1101 40-41 0.8601144504727003 1101 42-43 0.9319788102296869 1101 44-45 0.9781882110843796 1101 46-47 0.7790767329664376 1101 48-49 0.8034755513604068 1101 50-51 0.9440710866151178 1101 52-53 1.0352692484445427 1101 54-55 0.9873467170190224 1101 56-57 0.9456984957031906 1101 58-59 0.9972529545253508 1101 60-61 1.1057629768357309 1101 62-63 0.7911422614956223 1101 64-65 0.805439125565691 1101 66-67 0.9503157915477374 1101 68-69 0.8817186164790591 1101 70-71 1.0559353376462788 1101 72-73 1.0837552940808841 1101 74-75 0.8270597275568186 1101 76-77 1.0406544518593321 1101 78-79 1.2184744186516525 1101 80-81 1.1935454904388685 1101 82-83 1.3224439171674582 1101 84-85 1.222067924965227 1101 86-87 1.54599136012218 1101 88-89 1.465858480161863 1101 90-91 1.4633632882685603 1101 92-93 1.2203278364950094 1101 94-95 1.0316268727309286 1101 96-97 0.7499382608294276 1101 98-99 0.8912040483993007 1101 100-101 0.9241037387105262 1102 1 0.4220617312903663 1102 2 0.34222380852616396 1102 3 0.33641890240283345 1102 4 0.33915715278279635 1102 5 0.36284387784567684 1102 6 0.3718352028323224 1102 7 0.43382909037484296 1102 8 0.4650248783334092 1102 9 0.6750376737970072 1102 10-11 0.6603163378047299 1102 12-13 0.659607089504469 1102 14-15 0.7814353217149232 1102 16-17 0.7801859667318496 1102 18-19 0.7705445549973504 1102 20-21 0.6482878602675228 1102 22-23 0.683056656359895 1102 24-25 0.6899752185896659 1102 26-27 0.5417485463445999 1102 28-29 0.5015302051733599 1102 30-31 0.674282483800198 1102 32-33 0.6043015704940089 1102 34-35 0.4005736661814012 1102 36-37 0.7457530826916496 1102 38-39 0.6543303333971053 1102 40-41 0.7511735585710042 1102 42-43 0.9223407851882897 1102 44-45 0.8839466793231807 1102 46-47 0.9208652186640975 1102 48-49 0.9831828440102086 1102 50-51 1.0792589305158629 1102 52-53 0.9371639170519472 1102 54-55 0.847182766086334 1102 56-57 0.8642821178464217 1102 58-59 0.9606624837867841 1102 60-61 0.9256499203253234 1102 62-63 1.062007271021983 1102 64-65 1.176018349062602 1102 66-67 0.8725642682988486 1102 68-69 0.9388465222383893 1102 70-71 1.026765721480853 1102 72-73 1.0916015254685014 1102 74-75 0.9346122036612279 1102 76-77 1.1051669926492664 1102 78-79 1.1566023254608204 1102 80-81 1.3674100793604218 1102 82-83 1.3043964683260114 1102 84-85 1.4167050657535007 1102 86-87 1.5209437092339044 1102 88-89 1.6238874460609978 1102 90-91 1.5603334066210444 1102 92-93 1.5476986727865913 1102 94-95 1.4807135533019569 1102 96-97 1.4578488379761438 1102 98-99 1.6107332248871575 1102 100-101 1.4939491895884753 1103 1 0.4570101548837968 1103 2 0.4240242036392381 1103 3 0.3641904299504617 1103 4 0.3313830980485122 1103 5 0.20655271638542416 1103 6 0.41806184344384434 1103 7 0.3362355336662901 1103 8 0.44274711835185343 1103 9 0.5705132892168336 1103 10-11 0.734747711570833 1103 12-13 0.6565948788331681 1103 14-15 0.7520800073997762 1103 16-17 0.799167530831312 1103 18-19 0.859505358480142 1103 20-21 0.739171764120627 1103 22-23 0.7700565809876565 1103 24-25 0.827140121583021 1103 26-27 0.8612641910709229 1103 28-29 0.9830029527535942 1103 30-31 1.0064849565267195 1103 32-33 0.9864674747557487 1103 34-35 0.9910166978890942 1103 36-37 1.0270188891542276 1103 38-39 0.9772181928145827 1103 40-41 1.039767790673885 1103 42-43 1.169916219308334 1103 44-45 1.1652213069233852 1103 46-47 0.8432775864347875 1103 48-49 0.9024625533246251 1103 50-51 1.0318856805649048 1103 52-53 1.127217128370937 1103 54-55 1.148098095357696 1103 56-57 1.0838488697502129 1103 58-59 0.986721648403801 1103 60-61 1.3452619232796188 1103 62-63 1.3960248198208838 1103 64-65 1.3974065504261723 1103 66-67 1.3574395272523496 1103 68-69 1.4860057556879944 1103 70-71 1.5176675724233917 1103 72-73 1.6626768027477965 1103 74-75 1.7377992760669692 1103 76-77 1.6859617318890479 1103 78-79 1.5920765350407038 1103 80-81 1.6745069478288563 1103 82-83 1.6608726335197765 1103 84-85 1.9537003627478136 1103 86-87 2.0307192385298727 1103 88-89 2.178643803468738 1103 90-91 2.258177437454197 1103 92-93 2.0826353656524965 1103 94-95 2.0806247600077192 1103 96-97 2.371084682498008 1103 98-99 2.0806572559567744 1103 100-101 1.651477157674984 1104 1 0.2911147583504281 1104 2 0.30469609454916835 1104 3 0.22925576191843078 1104 4 0.1476042142893732 1104 5 0.2591361278220603 1104 6 0.3652007313684962 1104 7 0.3551144764368175 1104 8 0.4618422915676277 1104 9 0.5429016014912023 1104 10-11 0.5819832523349717 1104 12-13 0.5364239049138888 1104 14-15 0.6880307777297716 1104 16-17 0.7772045922957176 1104 18-19 0.7835307304832853 1104 20-21 0.7705116190798975 1104 22-23 0.7987611725941548 1104 24-25 0.732780120310224 1104 26-27 0.7298673134674729 1104 28-29 0.7438589505088444 1104 30-31 0.8580785532128274 1104 32-33 0.8786973891892345 1104 34-35 0.8177616920458064 1104 36-37 0.8249403288006292 1104 38-39 0.8539766019370489 1104 40-41 0.7902333369920385 1104 42-43 1.0753877173875708 1104 44-45 0.9649080258903382 1104 46-47 0.8934049020531276 1104 48-49 0.7734615669088214 1104 50-51 0.7955814204635487 1104 52-53 0.8832806205721724 1104 54-55 0.9821893200175111 1104 56-57 1.0909192075614698 1104 58-59 1.0579580092526442 1104 60-61 1.1468782639373138 1104 62-63 1.1165780660256424 1104 64-65 1.2693355718785462 1104 66-67 1.2012790206222483 1104 68-69 1.3329977660091998 1104 70-71 1.45003011060723 1104 72-73 1.4275325213560528 1104 74-75 1.3463890847427997 1104 76-77 1.1648320760642754 1104 78-79 1.2635921152036218 1104 80-81 1.119309787607154 1104 82-83 1.2478222221757065 1104 84-85 1.207326200188799 1104 86-87 1.4647496076981597 1104 88-89 1.351215586191131 1104 90-91 1.0742092616581225 1104 92-93 1.2547734219215876 1104 94-95 1.571196136303655 1104 96-97 1.6361183030779358 1104 98-99 1.6172987343076972 1104 100-101 1.2608489033706238 1105 1 0.4634904429635718 1105 2 0.3586751809476283 1105 3 0.3508762720322274 1105 4 0.38381657456669416 1105 5 0.38125679753891717 1105 6 0.4747453096243959 1105 7 0.49968947727683144 1105 8 0.398364547794948 1105 9 0.4163136210019758 1105 10-11 0.6088731311206459 1105 12-13 0.5847988587130004 1105 14-15 0.6391547018091188 1105 16-17 0.6105250920493148 1105 18-19 0.6208775325418898 1105 20-21 0.6667266639087543 1105 22-23 0.6741172169302061 1105 24-25 0.7270156846330593 1105 26-27 0.6715605689779878 1105 28-29 0.5527586063822412 1105 30-31 0.5986066713526981 1105 32-33 0.7192519153361374 1105 34-35 0.7384546318502601 1105 36-37 0.8651872515415349 1105 38-39 0.8286100398990612 1105 40-41 0.8968169458973279 1105 42-43 0.9748817126722784 1105 44-45 0.9564497634916975 1105 46-47 0.6853427947606789 1105 48-49 0.6663180347529973 1105 50-51 0.7100843164191488 1105 52-53 0.7966786328140145 1105 54-55 0.8525328306129154 1105 56-57 0.802712386490505 1105 58-59 0.9212882601934638 1105 60-61 0.8690468670744913 1105 62-63 1.0262761572289953 1105 64-65 1.002224268341518 1105 66-67 1.1572911385549105 1105 68-69 0.975655955046431 1105 70-71 1.0119364992098916 1105 72-73 1.0810942072404224 1105 74-75 1.3630582422906272 1105 76-77 1.3789477245847301 1105 78-79 1.3490235054863184 1105 80-81 1.4936991133822097 1105 82-83 1.6264743672968933 1105 84-85 1.6201495888978634 1105 86-87 1.7342601197999912 1105 88-89 1.7959325497568344 1105 90-91 1.788239333535504 1105 92-93 1.8133648093762282 1105 94-95 1.8056079745109486 1105 96-97 1.9034454732596089 1105 98-99 2.136693394185164 1105 100-101 1.792625138474337 1106 1 0.2410340058160081 1106 2 0.09339362948306729 1106 3 0.06255671931848283 1106 4 0.09869345484808889 1106 5 0.26294123647676315 1106 6 -0.02401890339530155 1106 7 0.2733318878748179 1106 8 0.2687854932994824 1106 9 0.11799575034842746 1106 10-11 0.1170194973260692 1106 12-13 0.009162031241032764 1106 14-15 0.33541240311222253 1106 16-17 0.1800172135939846 1106 18-19 0.0894355834127154 1106 20-21 0.1586921469271303 1106 22-23 0.16036525114120082 1106 24-25 0.10479270464819024 1106 26-27 0.025037091791332955 1106 28-29 0.2339523608737082 1106 30-31 -0.1421008968636599 1106 32-33 0.09268237424814885 1106 34-35 -0.25910720201370907 1106 36-37 -0.06338517722718962 1106 38-39 0.08042681185440159 1106 40-41 0.28625934726385793 1106 42-43 -0.2733598602147751 1106 44-45 -0.06143474587485542 1106 46-47 0.06003825629257875 1106 48-49 0.31996865857605883 1106 50-51 0.09452103283710755 1106 52-53 0.05064163060139748 1106 54-55 0.19050695592864741 1106 56-57 -0.7013356218931719 1106 58-59 -0.2404334865975315 1106 60-61 0.27154295451257937 1106 62-63 0.09315706615693031 1106 64-65 0.2469976941414842 1106 66-67 -0.1472797865591815 1106 68-69 -0.006126667456562984 1106 70-71 -0.14086509911033218 1106 72-73 0.2406890036065512 1106 74-75 0.6044815072403473 1106 76-77 0.5005671595888366 1106 78-79 0.6777602160438612 1106 80-81 0.7866450663505624 1106 82-83 0.5904563673511767 1106 84-85 0.6983950358824558 1106 86-87 0.3711309398242193 1106 88-89 0.46183986910434527 1106 90-91 0.7144646141223348 1106 92-93 0.48860860005879303 1106 94-95 0.6764966295594697 1106 96-97 0.6020089195157112 1106 98-99 0.5080413311893306 1106 100-101 0.02358406145793701 1107 1 0.08604081267858987 1107 2 -0.008883981723140977 1107 3 0.036673957386213374 1107 4 -0.13179645454101063 1107 5 -0.03408338186777371 1107 6 0.14660713303071304 1107 7 0.1509865238491841 1107 8 0.03225414755470268 1107 9 0.17365735500425217 1107 10-11 0.2970706528583449 1107 12-13 0.2724231555646526 1107 14-15 0.29184188553954726 1107 16-17 0.35440100589789125 1107 18-19 0.3639503970745821 1107 20-21 0.3995347610109192 1107 22-23 0.42722661118750693 1107 24-25 0.4524696505158232 1107 26-27 0.33336945259412687 1107 28-29 0.33939727210444204 1107 30-31 0.38445088947278805 1107 32-33 0.45130890745213037 1107 34-35 0.38672909466487937 1107 36-37 0.41135039448415256 1107 38-39 0.4534422856027547 1107 40-41 0.4606555249677058 1107 42-43 0.6696789619277368 1107 44-45 0.5779511834425577 1107 46-47 0.4945726925138558 1107 48-49 0.47086726403109225 1107 50-51 0.4715505678874905 1107 52-53 0.6106718339526438 1107 54-55 0.8130294184049305 1107 56-57 0.7382368630428218 1107 58-59 0.7237922858790142 1107 60-61 0.5859779125635285 1107 62-63 0.833915280762799 1107 64-65 0.7856417592834148 1107 66-67 0.7973267463094125 1107 68-69 0.9386996104975189 1107 70-71 1.228888673180304 1107 72-73 1.1421858749523786 1107 74-75 1.134061247211644 1107 76-77 1.2127700472512153 1107 78-79 1.3874972173963833 1107 80-81 1.4815063511481092 1107 82-83 1.2444345535548962 1107 84-85 1.2849583410664565 1107 86-87 1.318993556030975 1107 88-89 1.429000811119991 1107 90-91 1.5232969875658888 1107 92-93 1.4715942526582815 1107 94-95 1.6134116162594232 1107 96-97 1.5340254592596914 1107 98-99 1.5346781128648654 1107 100-101 1.392747207649153 1108 1 0.24514120065811795 1108 2 0.0435783060876318 1108 3 0.16044566576458763 1108 4 0.2052965219816798 1108 5 0.1554702624374471 1108 6 0.21435938521464948 1108 7 0.2313163995137444 1108 8 0.3176115223368612 1108 9 0.30311794764504896 1108 10-11 0.4374520023349717 1108 12-13 0.3879864049138888 1108 14-15 0.5386918354220782 1108 16-17 0.6184005057572577 1108 18-19 0.6098528458679056 1108 20-21 0.3708721960029706 1108 22-23 0.414145787978768 1108 24-25 0.5183870914640707 1108 26-27 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-2.5394432442669874 2316 80-81 -2.651275251472743 2316 82-83 -2.837857474299337 2316 84-85 -2.637458722984835 2316 86-87 -2.826830437594669 2316 88-89 -3.188447966929399 2316 90-91 -3.0488492442646695 2316 92-93 -3.181471949520631 2316 94-95 -3.6448191773173093 2316 96-97 -3.5218497307502794 2316 98-99 -3.224854944434128 2316 100-101 -2.968191715841389 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12245.0 3 4225.0 4 4499.0 5 5427.0 6 6574.0 7 8123.0 8 10235.0 9 12793.0 10 16257.0 11 19782.0 12 23423.0 13 27004.0 14 30572.0 15 35059.0 16 40204.0 17 45132.0 18 44805.0 19 44317.0 20 47273.0 21 51066.0 22 56302.0 23 62050.0 24 69613.0 25 77063.0 26 86106.0 27 97397.0 28 110582.0 29 124941.0 30 142685.0 31 165224.0 32 192170.0 33 226383.0 34 270330.0 35 317077.0 36 345031.0 37 286018.0 38 101064.0 39 3654.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.15406122914459 17.453580078728063 14.081803328797497 24.310555363329854 2 16.035101616072193 18.98612243630066 39.10217521297828 25.876600734648868 3 19.828117811124066 22.321385023496802 28.832050461190907 29.01844670418823 4 13.540996135253694 15.346180611648915 34.59013184874054 36.52269140435685 5 14.675018725311336 36.49689051054057 33.26967245467853 15.558418309469568 6 35.0720345089776 34.093704497000836 16.1582547720688 14.67600622195276 7 30.586810445194267 31.556235367295432 19.157292786315256 18.699661401195044 8 28.086883513331895 33.66863400257932 18.752058253421627 19.49242423066716 9 27.07772593869028 16.222668606701067 18.85853722730681 37.84106822730185 10-11 26.130602094687838 25.39747687445378 26.31528047922931 22.156640551629074 12-13 27.110202949998857 22.43620720996648 26.6246734431023 23.828916396932364 14-15 23.3467695064414 24.083444051269247 24.5731584959908 27.996627946298553 16-17 23.559830931512487 27.220374137127738 25.676120302217527 23.543674629142245 18-19 23.85215672804815 25.577241165550273 27.49493980301822 23.07566230338335 20-21 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27.407963230444178 23.599834101305266 46-47 24.044628560584304 25.941444798597495 26.560811782729505 23.453114858088693 48-49 23.651710202063867 26.104620256287475 26.30017344231639 23.94349609933227 50-51 23.85085521269879 26.133248179027618 26.538041460278418 23.47785514799517 52-53 24.281741376156198 25.52174889238771 26.657764287318038 23.538745444138062 54-55 23.444413686776183 25.98843318575859 26.857449379670932 23.709703747794293 56-57 23.55493320720848 26.151711685541944 26.55500706969515 23.73834803755442 58-59 23.501827950298125 25.55164000327121 26.64740150801438 24.299130538416286 60-61 22.924041696779785 25.959940463322727 27.084206594318626 24.03181124557886 62-63 23.324051711229572 25.926991986357645 27.227996620444756 23.520959681968023 64-65 22.935052965263413 26.048141211738475 26.9472240367801 24.069581786218013 66-67 22.82732828814783 26.1176639628395 26.95297153067982 24.102036218332852 68-69 23.696664006047836 25.506857196767037 27.131407053577743 23.665071743607378 70-71 23.328212803786357 25.810433216044338 26.535119031360114 24.326234948809187 72-73 23.288115980894986 25.985498042692036 26.609455914322925 24.116930062090056 74-75 23.321439662368938 26.440855046874496 26.47754327899988 23.760162011756687 76-77 23.562379933539496 25.920311020143778 26.520482315866023 23.99682673045071 78-79 23.60724251271052 26.23157967905942 26.508241355854782 23.65293645237528 80-81 23.95443161864634 26.255674286894365 26.09806576579386 23.691828328665434 82-83 23.713254089929972 26.296001093554622 26.277770011250517 23.71297480526489 84-85 23.47682073993898 26.585149087550924 26.246316565586852 23.691713606923244 86-87 24.055472858262082 26.484078490860686 25.76965766734254 23.690790983534697 88-89 23.694892188568033 26.677206407781817 25.485447893469352 24.142453510180793 90-91 23.535500368767273 26.609305972539875 25.780697081896136 24.074496576796715 92-93 23.884325225307716 26.075897700130827 26.061992019753514 23.977785054807935 94-95 23.58673129558822 25.75118548186933 26.32365032642759 24.338432896114863 96-97 24.026565767083575 25.29967433838975 26.267004647363933 24.406755247162742 98-99 23.7089257979572 25.811303560809122 25.687834202191784 24.79193643904189 100-101 24.399508738370574 24.94421292558411 26.207814227334268 24.448464108711043 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 366.0 1 257.0 2 236.0 3 346.5 4 330.0 5 318.5 6 534.0 7 1154.5 8 1870.5 9 2087.0 10 1836.0 11 1609.0 12 1585.0 13 1719.5 14 1903.5 15 2116.0 16 2380.5 17 2637.0 18 2962.5 19 3425.0 20 3956.0 21 4666.0 22 5785.0 23 7020.5 24 8615.0 25 10946.0 26 14021.0 27 17886.5 28 21185.0 29 24038.5 30 27687.5 31 34729.0 32 46135.0 33 49098.0 34 45721.0 35 47182.5 36 51179.5 37 56570.5 38 62590.5 39 67838.0 40 72674.5 41 79720.0 42 84629.5 43 87869.5 44 93128.5 45 102017.0 46 108811.5 47 113032.0 48 116795.5 49 117816.5 50 119301.0 51 121046.0 52 123649.5 53 129539.5 54 135875.5 55 132028.5 56 122973.0 57 115490.5 58 110088.0 59 99474.5 60 81391.5 61 68525.0 62 59555.5 63 46761.0 64 33980.5 65 26122.5 66 20125.5 67 15599.0 68 12274.0 69 9371.0 70 7179.0 71 5202.0 72 3703.5 73 2790.0 74 2122.0 75 1618.5 76 1261.5 77 933.5 78 635.0 79 408.5 80 262.5 81 184.5 82 129.0 83 79.5 84 54.0 85 34.5 86 27.0 87 23.0 88 11.5 89 6.0 90 7.5 91 7.5 92 4.0 93 2.0 94 1.5 95 3.0 96 3.5 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006826563399380333 2 0.10798382104474347 3 0.11654805512760244 4 0.11993030699365904 5 0.1593381957082637 6 0.09734058810843685 7 0.027864790602925182 8 0.028547446942863215 9 0.023861942064197623 10-11 0.05275071717702986 12-13 0.055496857453598766 14-15 0.02884223036238191 16-17 0.03264338498249142 18-19 0.03846147878878148 20-21 0.06013581758181404 22-23 0.03265889989930819 24-25 0.05023730065271255 26-27 0.0589877137373728 28-29 0.05479868619684395 30-31 0.06952234225596199 32-33 0.0517267326671228 34-35 0.06449550920732738 36-37 0.05486074586411104 38-39 0.03337258607287977 40-41 0.0379184567001944 42-43 0.03354325015786428 44-45 0.007959152327004799 46-47 0.014382327889149024 48-49 0.009557188759132468 50-51 0.00991403184591826 52-53 0.01072080752039048 54-55 0.011884426281648491 56-57 0.015545946650407034 58-59 0.020277996279522946 60-61 0.025615127664493025 62-63 0.012024060532999451 64-65 0.009619248426399562 66-67 0.011139710274443364 68-69 0.012318843952518149 70-71 0.032053818143454024 72-73 0.028950834780099327 74-75 0.029897244705922508 76-77 0.024560113320952426 78-79 0.005492280553137815 80-81 0.005073377799084931 82-83 0.00578706397265651 84-85 0.005244041884069439 86-87 0.01379276105011163 88-89 0.038694202541033076 90-91 0.03602563684854804 92-93 0.031029833633546975 94-95 0.04798763771428039 96-97 0.043953759341919287 98-99 0.026468448089415567 100-101 0.07524734656135142 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3222705.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.61097562770405 #Duplication Level Percentage of deduplicated Percentage of total 1 72.77840261857574 33.19493948059091 2 13.6399588170522 12.442636583349099 3 5.176194536990671 7.0827384861280915 4 2.562476330274167 4.675081817868143 5 1.4770337308335162 3.3684474749172155 6 0.9184596991366407 2.513510577140983 7 0.6222955684833827 1.9868455605126667 8 0.44858213953070275 1.6368215226526581 9 0.3349065885113574 1.374787462153413 >10 1.8472186539304354 15.247302167271013 >50 0.1207186517470471 3.80463467725735 >100 0.06309279959718464 5.263396686780638 >500 0.00594492490005321 1.781231291514642 >1k 0.004304945616349315 4.429157473944802 >5k 4.099948206046966E-4 1.1984687379185102 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7756 0.24066738966179035 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6765 0.20991682453094526 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6264 0.19437087788053825 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5944 0.18444133111780323 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5885 0.18261057093342392 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5839 0.18118319858628076 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4961 0.15393900465602653 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4950 0.15359767648605752 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4949 0.15356664665242398 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4521 0.14028587785726587 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4398 0.13646920832033957 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4244 0.13169061394077336 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3990 0.12380903619785243 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3693 0.11459317560868897 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3537 0.10975252156185565 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3357 0.10416715150781718 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3255 0.1010021084771954 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.8617900180128186E-4 2 3.1029833633546976E-5 0.0 0.0 0.0 1.8617900180128186E-4 3 3.1029833633546976E-5 0.0 0.0 0.0 1.8617900180128186E-4 4 3.1029833633546976E-5 0.0 0.0 0.0 2.172088354348288E-4 5 3.1029833633546976E-5 0.0 0.0 0.0 2.482386690683758E-4 6 3.1029833633546976E-5 0.0 0.0 0.0 3.1029833633546976E-4 7 3.1029833633546976E-5 0.0 0.0 0.0 3.1029833633546976E-4 8 3.1029833633546976E-5 0.0 0.0 0.0 3.4132816996901673E-4 9 3.1029833633546976E-5 0.0 0.0 0.0 3.4132816996901673E-4 10-11 3.1029833633546976E-5 0.0 0.0 0.0 4.189027540528841E-4 12-13 3.1029833633546976E-5 0.0 0.0 0.0 0.0011325889276244646 14-15 3.1029833633546976E-5 0.0 0.0 0.0 0.0013963425135096138 16-17 3.1029833633546976E-5 0.0 0.0 0.0 0.0016135513489444427 18-19 4.6544750450320464E-5 0.0 0.0 0.0 0.0017376706834786304 20-21 6.205966726709395E-5 0.0 0.0 1.5514916816773488E-5 0.0018928198516463654 22-23 6.205966726709395E-5 0.0 0.0 7.757458408386744E-5 0.0020169391861805533 24-25 6.205966726709395E-5 0.0 0.0 3.2581325315224325E-4 0.0020479690198141 26-27 6.205966726709395E-5 0.0 0.0 6.516265063044865E-4 0.002094513770264421 28-29 9.308950090064093E-5 0.0 0.0 0.00271511044293536 0.0021410585207147412 30-31 1.241193345341879E-4 0.0 0.0 0.010255360015887276 0.0021565734375315147 32-33 1.396342513509614E-4 0.0 0.0 0.02558409783085948 0.0022186331047986084 34-35 1.5514916816773488E-4 0.0 0.0 0.04946155481187388 0.002482386690683758 36-37 1.5514916816773488E-4 0.0 0.0 0.0905915992931404 0.002482386690683758 38-39 1.5514916816773488E-4 0.0 0.0 0.15365973615332462 0.0025289314411340784 40-41 1.5514916816773488E-4 0.0 0.0 0.2207927812195035 0.002544446357950852 42-43 1.5514916816773488E-4 0.0 0.0 0.2946593001841621 0.0025599612747676252 44-45 1.5514916816773488E-4 0.0 0.0 0.3856853171481721 0.002684080609301813 46-47 1.5514916816773488E-4 0.0 0.0 0.49523614479140965 0.002808199943836001 48-49 1.5514916816773488E-4 0.0 0.0 0.6112566927472418 0.0030719535297211503 50-51 1.5514916816773488E-4 0.0 0.0 0.741519934340872 0.0031650430306217913 52-53 1.5514916816773488E-4 0.0 0.0 0.8680906257321102 0.0033046772819727527 54-55 1.5514916816773488E-4 0.0 0.0 1.0061113257341272 0.003413281699690167 56-57 1.5514916816773488E-4 0.0 0.0 1.167016528040885 0.0034598264501404874 58-59 1.5514916816773488E-4 0.0 0.0 1.3386115080343997 0.003475341366957261 60-61 1.5514916816773488E-4 0.0 0.0 1.5269160534395794 0.0035529159510411285 62-63 1.8617900180128186E-4 0.0 0.0 1.7093869901216525 0.003630490535124996 64-65 1.8617900180128186E-4 0.0 0.0 1.9032458757472372 0.0037080651192088632 66-67 1.8617900180128186E-4 0.0 0.0 2.134262987148994 0.0037235800360256367 68-69 1.8617900180128186E-4 0.0 0.0 2.3875284892660047 0.0037235800360256367 70-71 1.8617900180128186E-4 0.0 0.0 2.648892777961371 0.003754609869659184 72-73 1.8617900180128186E-4 0.0 0.0 2.926920087317952 0.0040649082059946535 74-75 1.8617900180128186E-4 0.0 0.0 3.2063127093544086 0.004313146875063029 76-77 1.8617900180128186E-4 0.0 0.0 3.5082950502760877 0.00435969162551335 78-79 1.8617900180128186E-4 0.0 0.0 3.851531555013568 0.00440623637596367 80-81 1.8617900180128186E-4 0.0 0.0 4.210220916900554 0.0044682960432307645 82-83 1.8617900180128186E-4 0.0 0.0 4.590925945750541 0.004856168963650102 84-85 1.8617900180128186E-4 0.0 0.0 4.977774881659972 0.004918228630917195 86-87 1.8617900180128186E-4 0.0 0.0 5.374181006328534 0.005057862882268156 88-89 1.8617900180128186E-4 0.0 0.0 5.788087957166418 0.005181982216802344 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3950 0.0 54.78427 1 GTATCAA 8105 0.0 47.354595 1 ATCAACG 10660 0.0 35.69062 3 TCAACGC 10660 0.0 35.646008 4 TATCAAC 10740 0.0 35.513332 2 CAACGCA 10905 0.0 34.801548 5 AACGCAG 11485 0.0 33.2232 6 ACGCAGA 13180 0.0 28.846888 7 CGCAGAG 13300 0.0 28.515152 8 TAAGGTA 975 0.0 26.82745 4 TACATGG 10885 0.0 26.127274 2 GTACATG 11325 0.0 25.785275 1 TAAGGTG 2165 0.0 25.261625 5 GTGTAAG 1200 0.0 24.968985 1 GCAGAGT 15040 0.0 24.552223 9 ACATGGG 11215 0.0 23.450233 3 GTAAGGT 2240 0.0 23.354256 4 AGGTAAG 2140 0.0 23.112179 2 AAGGTAA 2060 0.0 23.087364 1 TGGTATC 1830 0.0 22.349545 2 >>END_MODULE