FastQCFastQC Report
Thu 26 May 2016
SRR938383_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938383_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3222705
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78210.2426843288479709No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA66420.20610015499401899No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA65140.202128336288925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63380.19666708556942072No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA62130.19278835636522734No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC57320.17786300638749125No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA55720.17289823300612375No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG52400.16259632823978615No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46380.14391636839239086No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA45530.14127883253353937No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA43600.1352900746422648No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA42840.13293180728611523No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG41730.1294874957527915No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA40030.12421242403508853No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT34800.10798382104474347No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT34310.10646335919669966No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT33580.10419818134145074No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG33460.10382582333784818No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG32760.10165373498349989No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45200.053.5228961
GTATCAA82850.043.1405371
TATCAAC110000.032.233422
TCAACGC111050.031.741184
ATCAACG111050.031.6984043
CAACGCA115600.030.4918525
AACGCAG121200.029.1095336
TAAGGTG23000.028.5028955
AGGTAAG22600.027.5500972
AAGGTAA23200.025.8132571
ACGCAGA139500.025.1547077
CGCAGAG141450.024.774368
GTAAGGT26300.024.7458614
TGGTATC21950.023.6021842
TACATGG106600.023.1403752
GTACATG109900.023.0509991
GTGGTAT24100.022.8771131
GGTAAGG27500.022.8023193
GTGTAAG13750.022.122671
TAAGGTA12200.021.8054944
Produced by FastQC (version 0.11.5)