FastQCFastQC Report
Thu 26 May 2016
SRR938382_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938382_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3255462
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80110.24607874396936594No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA69450.21333377566686387No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA66880.20543935085096987No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA61440.18872897303055602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60070.18452066096916506No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA60050.18445922575659No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA50660.15561539345260367No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG50070.15380305468163966No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC49720.15272793846157628No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA46070.1415160121666295No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA44900.13792205223098902No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43300.13300723522498495No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG40900.12563500971597888No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT37220.11433093060216953No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA36430.11190423970545503No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT33500.10290398106321008No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44350.055.290431
GTATCAA82850.045.631461
TATCAAC110400.034.2241252
ATCAACG111800.033.5404973
TCAACGC112800.033.2852864
CAACGCA116750.032.0762525
AACGCAG120300.031.1234746
ACGCAGA137400.027.0046397
CGCAGAG138300.026.8289018
TAAGGTG21850.026.5352175
TACATGG110150.025.4135342
GTACATG114250.025.380341
AGGTAAG21200.023.7631682
TAAGGTA11000.023.7631664
GTAAGGT23600.023.5617834
ACATGGG113200.023.1753853
GCAGAGT157100.023.0437349
AAGGTAA21450.022.8636881
TGGTATC20300.021.5390262
GGTAAGG25400.021.5178283
Produced by FastQC (version 0.11.5)