##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938382_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3255462 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.013711417918564 31.0 31.0 34.0 28.0 34.0 2 31.108690870911715 31.0 31.0 34.0 27.0 34.0 3 31.17720280562329 31.0 31.0 34.0 27.0 34.0 4 34.81637997924719 37.0 35.0 37.0 32.0 37.0 5 34.82023718906871 37.0 35.0 37.0 32.0 37.0 6 34.855869305186175 37.0 35.0 37.0 32.0 37.0 7 34.76784247520014 37.0 35.0 37.0 32.0 37.0 8 34.718841442474215 37.0 35.0 37.0 31.0 37.0 9 36.22190705958171 39.0 35.0 39.0 32.0 39.0 10-11 36.067622045657416 39.0 35.0 39.0 30.0 39.0 12-13 35.909333606105676 38.0 35.0 39.0 30.0 39.0 14-15 36.89095188332716 39.0 36.0 41.0 30.5 41.0 16-17 36.72775707411114 39.0 36.0 41.0 30.0 41.0 18-19 36.69970022688024 39.0 36.0 40.5 30.0 41.0 20-21 36.7675051958831 39.0 36.0 41.0 30.0 41.0 22-23 36.69480491555423 39.0 36.0 41.0 30.0 41.0 24-25 36.55438982239694 39.0 36.0 41.0 30.0 41.0 26-27 36.349306335014816 39.0 35.5 41.0 29.0 41.0 28-29 36.16127173347439 39.0 35.0 40.5 29.0 41.0 30-31 35.920078164020964 38.5 35.0 40.0 27.5 41.0 32-33 35.62662903145544 38.0 34.5 40.0 27.0 41.0 34-35 35.37111199577817 38.0 34.0 40.0 26.5 41.0 36-37 35.05686151458687 38.0 34.0 40.0 25.0 41.0 38-39 34.76843517141346 38.0 33.5 40.0 24.5 41.0 40-41 34.67876018826206 38.0 33.0 40.0 24.0 41.0 42-43 34.46649031688897 38.0 33.0 40.0 24.0 41.0 44-45 34.1670424965796 38.0 33.0 40.0 23.0 41.0 46-47 33.79495291298132 38.0 33.0 40.0 22.5 41.0 48-49 33.39318873941701 37.5 32.0 40.0 19.5 41.0 50-51 32.62014899882106 36.5 31.0 39.5 18.0 40.5 52-53 32.656125459305 37.0 31.0 39.0 18.0 40.0 54-55 32.741223365531525 37.0 31.0 40.0 16.5 41.0 56-57 32.39447181383164 36.0 31.0 39.0 13.5 41.0 58-59 32.0143188585829 36.0 31.0 39.0 10.5 41.0 60-61 31.524895851955883 35.0 30.0 39.0 8.0 40.0 62-63 30.96553208116083 35.0 29.5 38.5 6.5 40.0 64-65 30.423088643025167 35.0 29.0 38.0 2.0 40.0 66-67 29.850529510097182 34.0 28.0 37.5 2.0 40.0 68-69 29.260508800287027 34.0 27.5 37.0 2.0 39.0 70-71 28.597048898128744 34.0 26.0 36.0 2.0 39.0 72-73 28.027935051921972 33.0 26.0 36.0 2.0 38.5 74-75 27.398417336771246 33.0 25.0 35.0 2.0 37.5 76-77 26.6531526093685 32.5 23.5 35.0 2.0 37.0 78-79 25.85839321730679 32.0 21.0 35.0 2.0 36.0 80-81 25.199445270748054 31.0 19.0 34.5 2.0 36.0 82-83 24.528106916929147 31.0 16.5 34.0 2.0 35.5 84-85 23.867287653795376 30.5 9.0 34.0 2.0 35.0 86-87 23.188551271678183 30.0 4.5 34.0 2.0 35.0 88-89 22.34914675705015 29.5 2.0 34.0 2.0 35.0 90-91 21.48634218430441 29.0 2.0 34.0 2.0 35.0 92-93 20.61477311054468 28.0 2.0 34.0 2.0 35.0 94-95 19.62178163959524 26.5 2.0 33.0 2.0 35.0 96-97 18.3609039208567 24.5 2.0 33.0 2.0 34.5 98-99 16.852968641624443 13.5 2.0 32.0 2.0 34.0 100-101 13.555450654930084 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7852939269404473 1101 2 0.7177923964485906 1101 3 0.8221334333038897 1101 4 0.7266115275280427 1101 5 0.6337665634978507 1101 6 0.6835963074923299 1101 7 0.6856766294812644 1101 8 0.7132499825134033 1101 9 0.8382302742317904 1101 10-11 0.8255900847340243 1101 12-13 0.8070617226085304 1101 14-15 1.0403207045906768 1101 16-17 1.0426374241556715 1101 18-19 0.9113327488978271 1101 20-21 1.055700901387894 1101 22-23 1.0405729751232684 1101 24-25 1.1007407036777934 1101 26-27 1.1342437990352394 1101 28-29 1.1833276213802648 1101 30-31 1.0913336316696345 1101 32-33 1.1936583231695224 1101 34-35 1.0884386617343225 1101 36-37 1.1586063400417004 1101 38-39 1.2262102703693714 1101 40-41 1.258315460601679 1101 42-43 1.263040809151569 1101 44-45 1.2578746925681088 1101 46-47 1.404221230605927 1101 48-49 1.3944619180514906 1101 50-51 1.2822837378544065 1101 52-53 1.4287969916462586 1101 54-55 1.40574934590974 1101 56-57 1.4034767719319845 1101 58-59 1.387152158175006 1101 60-61 1.3520137735719295 1101 62-63 1.3535520224839566 1101 64-65 1.286277097705156 1101 66-67 1.3465076810052174 1101 68-69 1.5350645263662273 1101 70-71 1.6629941295080286 1101 72-73 1.6949495410045188 1101 74-75 1.488196754200029 1101 76-77 1.523794664077311 1101 78-79 1.4566006474011814 1101 80-81 1.6242859326669894 1101 82-83 1.6992697639053667 1101 84-85 1.7826597012564953 1101 86-87 1.8509213491540386 1101 88-89 2.028106575556645 1101 90-91 2.100839980762572 1101 92-93 1.9123418412472084 1101 94-95 1.844332765156942 1101 96-97 1.7609264984081214 1101 98-99 1.7111598609901755 1101 100-101 1.4388578379189259 1102 1 0.5960048106855282 1102 2 0.6349516051790332 1102 3 0.5131087412296793 1102 4 0.5340275963384968 1102 5 0.6257283601597834 1102 6 0.554848907829097 1102 7 0.4592496306114384 1102 8 0.49495916315482447 1102 9 0.6190799666886448 1102 10-11 0.6517725116444595 1102 12-13 0.7416157647350659 1102 14-15 0.7067091422306788 1102 16-17 0.7062096373193256 1102 18-19 0.8669317801336192 1102 20-21 0.7121672437852311 1102 22-23 0.7950748716283087 1102 24-25 0.793280843202659 1102 26-27 0.7480392032441117 1102 28-29 0.6988044554479274 1102 30-31 0.6568613150877312 1102 32-33 0.6563551943377561 1102 34-35 0.7941084115483434 1102 36-37 0.7370150085804639 1102 38-39 0.887879978989929 1102 40-41 0.804710532655271 1102 42-43 0.8566013936677805 1102 44-45 1.0303587126259117 1102 46-47 0.9284497839806463 1102 48-49 0.6834677253836645 1102 50-51 0.7782549519445325 1102 52-53 0.806418799082742 1102 54-55 0.7325627601611302 1102 56-57 0.8006272053230177 1102 58-59 1.0497683241868927 1102 60-61 0.853567881645791 1102 62-63 0.6404306508855555 1102 64-65 0.8908478609726167 1102 66-67 0.9474433109428766 1102 68-69 0.9223641027350133 1102 70-71 0.9405527862461902 1102 72-73 1.0940730085560766 1102 74-75 0.925282162401647 1102 76-77 0.9523148572783846 1102 78-79 0.9867702227099002 1102 80-81 0.9753214283857972 1102 82-83 1.0561280393395371 1102 84-85 1.1097419594816316 1102 86-87 1.1034381180075563 1102 88-89 1.1404841596664 1102 90-91 1.2457508035489013 1102 92-93 1.2956502821448979 1102 94-95 1.2258112152173446 1102 96-97 1.2461681877705537 1102 98-99 1.3646479922680435 1102 100-101 1.078479600760657 1103 1 0.6187269800536619 1103 2 0.5462506068276873 1103 3 0.5252974766119145 1103 4 0.4883865936143792 1103 5 0.5054944611235683 1103 6 0.46627089371803976 1103 7 0.3809112548011413 1103 8 0.40939555555801377 1103 9 0.533737213673497 1103 10-11 0.431161682531247 1103 12-13 0.4633973447243207 1103 14-15 0.6633436249434581 1103 16-17 0.7128081295990185 1103 18-19 0.7786072132745048 1103 20-21 0.6775204966260588 1103 22-23 0.7710364384992303 1103 24-25 0.8787304226187231 1103 26-27 0.7197431332738375 1103 28-29 0.6522375623622239 1103 30-31 0.8356358048323997 1103 32-33 0.8915282069419774 1103 34-35 0.8879500034149785 1103 36-37 0.9357899488980195 1103 38-39 0.9302034541330784 1103 40-41 0.9548313563616091 1103 42-43 0.9594000607106565 1103 44-45 1.0694000227737064 1103 46-47 0.9889095566850798 1103 48-49 1.210943346435613 1103 50-51 1.241824933301153 1103 52-53 1.0898775295603755 1103 54-55 1.0582099785752348 1103 56-57 1.2029433715829398 1103 58-59 1.1462039417524394 1103 60-61 1.112004951385611 1103 62-63 1.3326583423568756 1103 64-65 1.3843398525934383 1103 66-67 1.4450681085543984 1103 68-69 1.4116864484913592 1103 70-71 1.366302713120426 1103 72-73 1.297178249826782 1103 74-75 1.2353714127665363 1103 76-77 1.1720106910302839 1103 78-79 1.1758537832994023 1103 80-81 1.3440287347576358 1103 82-83 1.2857392466385242 1103 84-85 1.2283370842829342 1103 86-87 1.2497159611963689 1103 88-89 1.3740408343498167 1103 90-91 1.4201467034833115 1103 92-93 1.3419248568336322 1103 94-95 1.2687416711380166 1103 96-97 1.315901359980991 1103 98-99 1.3308137536032767 1103 100-101 1.1809451393208317 1104 1 0.5800899904509649 1104 2 0.5639961655965386 1104 3 0.4889083582020355 1104 4 0.5424079448044452 1104 5 0.48859741276349666 1104 6 0.6119586246735338 1104 7 0.6077152897801454 1104 8 0.4634068652047816 1104 9 0.6184182655681951 1104 10-11 0.5949148671349818 1104 12-13 0.6081117632328912 1104 14-15 0.622004384313648 1104 16-17 0.7519651276939925 1104 18-19 0.7352668365157982 1104 20-21 0.8649398056531226 1104 22-23 0.8592413548388365 1104 24-25 0.878857017789592 1104 26-27 0.925643902742955 1104 28-29 0.8339557851812032 1104 30-31 0.7675466101067911 1104 32-33 0.700940432377493 1104 34-35 0.7265429536777859 1104 36-37 0.7802152426848892 1104 38-39 0.745971617850401 1104 40-41 0.8308401728366235 1104 42-43 0.7128009162501527 1104 44-45 0.8519206366275895 1104 46-47 0.8488691179557151 1104 48-49 0.7531440080298637 1104 50-51 0.653131208978813 1104 52-53 0.7720641186489487 1104 54-55 0.9861873678887321 1104 56-57 0.9499280395808256 1104 58-59 0.8973296704815894 1104 60-61 0.8611991175358575 1104 62-63 1.0717274052226777 1104 64-65 0.9842539256729204 1104 66-67 0.9741215209577128 1104 68-69 1.0674300678078446 1104 70-71 1.0341589440414936 1104 72-73 0.8686184510019288 1104 74-75 0.72718253420609 1104 76-77 0.7400959408638812 1104 78-79 0.8763536369797684 1104 80-81 1.077993107362012 1104 82-83 0.8837361022009667 1104 84-85 0.8497884746063313 1104 86-87 1.0383941928519071 1104 88-89 1.195439076219035 1104 90-91 1.1845885945067494 1104 92-93 1.2994935983700486 1104 94-95 1.106038186435594 1104 96-97 1.0994888878104092 1104 98-99 1.066251722686797 1104 100-101 0.9345726468376458 1105 1 0.4833329153457626 1105 2 0.5879719613710535 1105 3 0.45160853203652707 1105 4 0.4866923330582864 1105 5 0.38623500410948 1105 6 0.4919923659688621 1105 7 0.3154620094052092 1105 8 0.40829780944307004 1105 9 0.490079358757157 1105 10-11 0.4615224062477452 1105 12-13 0.47557474829638835 1105 14-15 0.6334463300167883 1105 16-17 0.5945404110815034 1105 18-19 0.606060181700542 1105 20-21 0.6475073555813253 1105 22-23 0.5957438007460567 1105 24-25 0.5969597355333534 1105 26-27 0.6547103400475507 1105 28-29 0.6429361029315928 1105 30-31 0.5961244875441523 1105 32-33 0.6565551413860149 1105 34-35 0.6502976750764162 1105 36-37 0.7449385215887503 1105 38-39 0.6639316835718603 1105 40-41 0.6174729687126614 1105 42-43 0.6669999826408386 1105 44-45 0.6164142685667287 1105 46-47 0.5695608352291472 1105 48-49 0.40611369784200235 1105 50-51 0.30051473054651723 1105 52-53 0.25918995492958885 1105 54-55 0.290425826749626 1105 56-57 0.4150201210986353 1105 58-59 0.371304870577724 1105 60-61 0.48252714881362735 1105 62-63 0.4991597931367231 1105 64-65 0.5438071306297907 1105 66-67 0.5605742665688602 1105 68-69 0.42537268792250416 1105 70-71 0.5281581318469719 1105 72-73 0.62559734119613 1105 74-75 0.44343644755405265 1105 76-77 0.5082334410451743 1105 78-79 0.6397226022398073 1105 80-81 0.6890673123126696 1105 82-83 0.7763705131423855 1105 84-85 0.6801002941085308 1105 86-87 0.890965218433962 1105 88-89 0.8770159612044175 1105 90-91 0.9119739238288318 1105 92-93 1.048641130811209 1105 94-95 1.162134125877376 1105 96-97 1.2183023793559187 1105 98-99 1.0967129020678321 1105 100-101 0.8998502951201228 1106 1 0.3529050138186882 1106 2 0.5323174686463084 1106 3 0.19996530154422842 1106 4 0.4009366390252893 1106 5 0.4678016084561065 1106 6 0.028392768843779947 1106 7 0.2655639475532183 1106 8 0.36986668657711874 1106 9 0.37240579018513387 1106 10-11 0.2600832596119531 1106 12-13 0.3054485670825855 1106 14-15 0.6119544785823621 1106 16-17 0.45691638719959116 1106 18-19 0.14736887137632948 1106 20-21 0.35405586324731786 1106 22-23 0.46809967463022417 1106 24-25 0.4802567717251591 1106 26-27 0.38094998213023246 1106 28-29 0.4292493303986973 1106 30-31 0.4716969004712226 1106 32-33 0.6591966081330085 1106 34-35 0.5647944264952258 1106 36-37 0.49053551486743174 1106 38-39 0.6565843015064345 1106 40-41 0.7399514330140704 1106 42-43 0.277496757929093 1106 44-45 0.6281258694860128 1106 46-47 0.35311934575427983 1106 48-49 0.7013329569651887 1106 50-51 0.33125236422196025 1106 52-53 0.739833771261388 1106 54-55 0.5512619115567716 1106 56-57 0.04260872478210587 1106 58-59 -0.3596347194591658 1106 60-61 0.5146214497732338 1106 62-63 0.6280146455638054 1106 64-65 0.7826052204505594 1106 66-67 0.12949667474798687 1106 68-69 0.13489203489567458 1106 70-71 0.3089364992695245 1106 72-73 0.6238837995862205 1106 74-75 0.6619305805972182 1106 76-77 0.5817940479313073 1106 78-79 0.8310030896333593 1106 80-81 0.7302016511728162 1106 82-83 0.6413993718346838 1106 84-85 0.5597371306997836 1106 86-87 0.7738384420404394 1106 88-89 0.44622298671204774 1106 90-91 0.5073972668085425 1106 92-93 0.6026050433057826 1106 94-95 0.47811695854884917 1106 96-97 0.37296893601552483 1106 98-99 0.17975681823622125 1106 100-101 0.05340056549361272 1107 1 0.442342122253887 1107 2 0.38414102107454795 1107 3 0.37055582062052395 1107 4 0.43925694571867524 1107 5 0.3975471785464535 1107 6 0.42228837788496776 1107 7 0.33267986921013915 1107 8 0.32482704310598365 1107 9 0.36035650678109477 1107 10-11 0.4581069577873862 1107 12-13 0.45873561496976123 1107 14-15 0.44615051782915316 1107 16-17 0.4737201706197496 1107 18-19 0.5211705023342645 1107 20-21 0.5908467440425511 1107 22-23 0.6055638811086723 1107 24-25 0.5175933102142736 1107 26-27 0.4446667952443093 1107 28-29 0.5686523051733658 1107 30-31 0.6479037818374351 1107 32-33 0.4322185641925458 1107 34-35 0.5321055832416164 1107 36-37 0.6988893697354257 1107 38-39 0.6832659242169399 1107 40-41 0.6471587056603596 1107 42-43 0.6748357244131498 1107 44-45 0.7898108439768805 1107 46-47 0.7324941446516746 1107 48-49 0.7530117914466317 1107 50-51 0.7281450875093398 1107 52-53 0.6714069601231145 1107 54-55 0.6607277847297226 1107 56-57 0.6322487707368438 1107 58-59 0.7083733511352506 1107 60-61 0.5850245289904912 1107 62-63 0.8078684124401825 1107 64-65 0.593813428861953 1107 66-67 0.6409231411750937 1107 68-69 0.5308662033242832 1107 70-71 0.7249387153421765 1107 72-73 0.5877315202659013 1107 74-75 0.5563189173801923 1107 76-77 0.8572221064751737 1107 78-79 0.8036248736578955 1107 80-81 0.6563365278942861 1107 82-83 0.7394729881686253 1107 84-85 0.7440213977819248 1107 86-87 0.5616993025765105 1107 88-89 0.6357114137475968 1107 90-91 0.8894208411376638 1107 92-93 0.7990040180473805 1107 94-95 0.8094312934545549 1107 96-97 0.7617406995986755 1107 98-99 0.6819766872662285 1107 100-101 0.3817281207389325 1108 1 0.29578722843248073 1108 2 0.39953872188145567 1108 3 0.36003031693295995 1108 4 0.33585341832917237 1108 5 0.3184729813526914 1108 6 0.3718064658390787 1108 7 0.27963047211708414 1108 8 0.28854158398127083 1108 9 0.25681401052401753 1108 10-11 0.40296780702555424 1108 12-13 0.44019934745146116 1108 14-15 0.5129693928343215 1108 16-17 0.3770308200684198 1108 18-19 0.4985247091174543 1108 20-21 0.4797322675884885 1108 22-23 0.5056362741931721 1108 24-25 0.502824242579365 1108 26-27 0.6539168265889543 1108 28-29 0.28798371161536807 1108 30-31 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-1.2001694581200546 2316 64-65 -1.3885138649607818 2316 66-67 -1.282088013576331 2316 68-69 -1.096667957001408 2316 70-71 -1.4762153676504255 2316 72-73 -1.3485907136244322 2316 74-75 -1.2695468441673192 2316 76-77 -1.0633899212499394 2316 78-79 -1.0726131378249377 2316 80-81 -1.1740040035063721 2316 82-83 -1.0276977152099853 2316 84-85 -1.1596356776037773 2316 86-87 -1.2155089600115367 2316 88-89 -1.2197984223184086 2316 90-91 -1.4652142516022089 2316 92-93 -1.5578293655570512 2316 94-95 -1.535930556365198 2316 96-97 -1.4769101299357104 2316 98-99 -1.4674582951254571 2316 100-101 -1.1467885116928702 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11299.0 3 4194.0 4 4503.0 5 5086.0 6 6074.0 7 7462.0 8 9646.0 9 12367.0 10 15019.0 11 18549.0 12 22453.0 13 25478.0 14 29003.0 15 32899.0 16 37668.0 17 42521.0 18 42077.0 19 41437.0 20 43655.0 21 47271.0 22 51986.0 23 57732.0 24 64116.0 25 71818.0 26 81206.0 27 91730.0 28 104593.0 29 119857.0 30 137619.0 31 160618.0 32 187859.0 33 224815.0 34 273353.0 35 330998.0 36 373815.0 37 329362.0 38 129407.0 39 5916.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.26195707700167 17.432282723093113 14.07540759615207 24.230352603753147 2 16.047558109231094 18.915679535027806 39.164324841296775 25.87243751444433 3 19.780787480946056 22.40365466883021 28.790197669272754 29.025360180950976 4 13.55252611184327 15.34166068372706 34.613344332107246 36.49246887232243 5 14.687697288870558 36.496635293061765 33.26975009512897 15.545917322938713 6 35.0435875533337 34.08856813788427 16.162297358066503 14.705546950715526 7 30.530525978094563 31.601921296417217 19.177072767058327 18.690479958429886 8 28.045132790135412 33.68098532775631 18.754647685468 19.51923419664027 9 27.07144255380218 16.208766978980652 18.853586724673544 37.86620374254363 10-11 26.099443955140266 25.42786628617718 26.319215968757742 22.153473789924814 12-13 27.08727827652308 22.472117279819162 26.59232811110435 23.848276332553407 14-15 23.31563372880887 24.09083645995618 24.582891611432224 28.010638199802724 16-17 23.574748582649427 27.24455003142669 25.64072112228385 23.539980263640036 18-19 23.83586110170424 25.565744000993202 27.490733291499215 23.107661605803347 20-21 23.769080706962907 24.930914913512066 27.967938803436926 23.3320655760881 22-23 23.649317392376645 24.773405308888687 28.028639632807884 23.54863766592678 24-25 23.597331989840193 25.113249647809504 27.88432720779562 23.405091154554686 26-27 22.912709623406272 25.52564901656334 27.628368020083034 23.93327333994736 28-29 23.245439889267843 26.179079883146105 27.355470749955902 23.22000947763015 30-31 23.224336744000496 25.46733075890277 27.281316189791543 24.027016307305193 32-33 22.76927114802929 25.461337229493374 27.395305553318856 24.37408606915848 34-35 23.78728037757432 25.616129088767277 27.14703596354196 23.449554570116447 36-37 24.08970424857441 24.93122899645504 27.145331085784356 23.833735669186197 38-39 23.34942639842365 25.432624666972487 27.11483799446385 24.103110940140006 40-41 23.84062354919517 25.212871763216977 27.371592538573164 23.57491214901469 42-43 23.58086498356021 25.283081939641853 27.017067795107803 24.11898528169013 44-45 23.364008258484038 25.556189731328942 27.448369883258756 23.631432126928267 46-47 24.043046693312277 25.935233343676174 26.565880303649386 23.455839659362166 48-49 23.612837680807242 26.115165189774352 26.333300512935203 23.938696616483202 50-51 23.82241105077097 26.178103517264528 26.513356112569053 23.486129319395452 52-53 24.25245775886553 25.550569781642967 26.65789185476985 23.539080604721654 54-55 23.40772547087807 25.99692699076269 26.88265593033488 23.712691608024354 56-57 23.509024374659006 26.173589572816773 26.628156810797794 23.68922924172643 58-59 23.510255479570834 25.539278430690665 26.698055229103378 24.25241086063512 60-61 22.91773038141355 25.951014745467273 27.110671186340706 24.02058368677848 62-63 23.279046374639456 25.90930643012701 27.24342842011603 23.568218775117504 64-65 22.902606308328135 26.099562965701573 26.95120615660337 24.046624569366926 66-67 22.840738531256164 26.08858529068978 26.986621309202214 24.084054868851844 68-69 23.69258261532322 25.50100962251822 27.156203794755246 23.650203967403307 70-71 23.297882146181994 25.83545077748862 26.55176651683253 24.314900559496852 72-73 23.26020031952808 26.019402113801153 26.614968661668918 24.105428905001844 74-75 23.310390277736172 26.47565631963279 26.526750128080163 23.687203274550875 76-77 23.556949011484132 25.9419920975258 26.552211920131036 23.94884697085903 78-79 23.593682657751543 26.285790557101922 26.533863464824826 23.586663320321705 80-81 23.944904173658603 26.23710251556146 26.16495724198311 23.653036068796823 82-83 23.660517763662543 26.34957356826732 26.335749825897647 23.65415884217249 84-85 23.468434206904686 26.628285108328093 26.27099041070305 23.63229027406417 86-87 24.02265794644773 26.529958971717043 25.829739460956773 23.61764362087845 88-89 23.651389553780643 26.70179855695103 25.56781414522301 24.07899774404532 90-91 23.51263013905314 26.69375321789796 25.824722951565477 23.968893691483423 92-93 23.905248450520745 26.105681820800392 26.099060672607266 23.8900090560716 94-95 23.563761328982547 25.833698348666022 26.41656548405796 24.18597483829347 96-97 24.005632582322356 25.33248521946483 26.332875474759394 24.32900672345342 98-99 23.664800178199723 25.865873985144905 25.77233449316773 24.696991343487646 100-101 24.29655480395371 25.048916998264765 26.23736477471731 24.41716342306422 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 409.0 1 295.5 2 265.5 3 364.5 4 319.0 5 311.0 6 544.5 7 1200.0 8 1938.0 9 2175.5 10 1923.0 11 1658.0 12 1694.0 13 1870.5 14 2061.5 15 2301.5 16 2497.0 17 2719.0 18 3061.0 19 3484.5 20 4062.5 21 4911.0 22 6007.0 23 7289.5 24 8895.5 25 11203.5 26 14280.5 27 18010.5 28 21277.0 29 24080.5 30 27793.5 31 35019.0 32 46882.5 33 49827.0 34 45747.5 35 47176.5 36 51745.5 37 57243.5 38 63218.0 39 68683.5 40 73888.5 41 81201.5 42 85912.0 43 89217.5 44 94705.5 45 103512.0 46 110260.5 47 114541.5 48 118047.0 49 119108.5 50 120826.5 51 121913.0 52 124556.0 53 130594.0 54 136918.5 55 133311.5 56 123951.0 57 116403.0 58 110589.0 59 99579.0 60 81550.5 61 68897.5 62 59901.5 63 46812.0 64 34028.0 65 26118.5 66 20229.5 67 15847.0 68 12399.0 69 9535.0 70 7304.5 71 5200.5 72 3763.0 73 2862.0 74 2158.5 75 1635.5 76 1231.0 77 904.5 78 620.5 79 399.0 80 263.5 81 170.0 82 111.5 83 74.0 84 52.0 85 37.5 86 21.5 87 12.5 88 9.5 89 5.5 90 4.5 91 5.0 92 4.5 93 5.0 94 2.0 95 2.0 96 3.0 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1819096644347254 2 0.04884099399716538 3 0.04736654889536416 4 0.054247292703769844 5 0.06094373087445038 6 0.06527491336099146 7 0.036891845151318 8 0.03544811765580431 9 0.041929532582472166 10-11 0.04325038965283576 12-13 0.039810017748632916 14-15 0.035386682443229255 16-17 0.03263745668049573 18-19 0.041161592425284034 20-21 0.05174380779133653 22-23 0.04851845913114636 24-25 0.06914533175321967 26-27 0.05437016312891994 28-29 0.04558492773068769 30-31 0.055214897301826896 32-33 0.05099122643729216 34-35 0.05034615670525413 36-37 0.047520136926801786 38-39 0.026140682950684113 40-41 0.02466623784888289 42-43 0.021701988842136692 44-45 0.004392617699116132 46-47 0.012041301664709955 48-49 0.01144230834210321 50-51 0.009353511114551483 52-53 0.009568534358564161 54-55 0.01064365057862755 56-57 0.008539494547932059 58-59 0.010705085791202601 60-61 0.011058338263509142 62-63 0.004223670864534742 64-65 0.006926820217836976 66-67 0.004177594455103454 68-69 0.008754517791944739 70-71 0.023099639928219097 72-73 0.02033505536234181 74-75 0.020411849378060627 76-77 0.012455989349591549 78-79 0.005191275462591792 80-81 0.006896102611549451 82-83 0.005928498013492401 84-85 0.005099122643729215 86-87 0.008247677288200568 88-89 0.029611772461174482 90-91 0.036937921560749286 92-93 0.02271566984962503 94-95 0.03171592849186997 96-97 0.03630821063185502 98-99 0.021102995519529946 100-101 0.1239762589764525 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3255462.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.97844015300423 #Duplication Level Percentage of deduplicated Percentage of total 1 72.35800499842422 32.54550197412405 2 13.6821157465778 12.308004485478525 3 5.2214559530936775 7.045588322933148 4 2.6797494624385365 4.821238032853478 5 1.5457187760634927 3.4762009731273373 6 0.9576095923741188 2.5843071444325245 7 0.644374716438512 2.0288078733607073 8 0.46007152497115683 1.6554639641613256 9 0.3527019742222337 1.4277586175461063 >10 1.896251595542859 15.377464376366293 >50 0.1252277285871893 3.86577916399756 >100 0.06579487881052236 5.356020146583567 >500 0.00604546299507421 1.7671417259082653 >1k 0.0043280019157227526 4.199673028178227 >5k 5.495875448536828E-4 1.5410501709487816 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8011 0.24607874396936594 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6945 0.21333377566686387 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6688 0.20543935085096987 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6144 0.18872897303055602 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6007 0.18452066096916506 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 6005 0.18445922575659 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 5066 0.15561539345260367 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5007 0.15380305468163966 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4972 0.15272793846157628 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4607 0.1415160121666295 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4490 0.13792205223098902 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4330 0.13300723522498495 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4090 0.12563500971597888 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3722 0.11433093060216953 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3643 0.11190423970545503 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3350 0.10290398106321008 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.53588031437627E-4 2 0.0 0.0 0.0 0.0 1.53588031437627E-4 3 0.0 0.0 0.0 0.0 1.53588031437627E-4 4 0.0 0.0 0.0 0.0 1.8430563772515237E-4 5 0.0 0.0 0.0 0.0 2.1502324401267777E-4 6 0.0 0.0 0.0 0.0 2.1502324401267777E-4 7 0.0 0.0 0.0 0.0 2.1502324401267777E-4 8 0.0 0.0 0.0 0.0 2.457408503002032E-4 9 0.0 0.0 0.0 0.0 2.457408503002032E-4 10-11 1.53588031437627E-5 0.0 0.0 0.0 3.3789366916277936E-4 12-13 6.14352125750508E-5 0.0 0.0 0.0 9.215281886257619E-4 14-15 6.14352125750508E-5 0.0 0.0 0.0 0.0011365514326384398 16-17 6.14352125750508E-5 0.0 0.0 6.14352125750508E-5 0.0013362158735073547 18-19 6.14352125750508E-5 0.0 0.0 1.0751162200633889E-4 0.0014437274955136936 20-21 6.14352125750508E-5 0.0 0.0 1.6894683458138968E-4 0.0017969799678202356 22-23 6.14352125750508E-5 0.0 0.0 2.764584565877286E-4 0.0019352091961141 24-25 6.14352125750508E-5 0.0 0.0 4.607640943128809E-4 0.0019659268024016254 26-27 6.14352125750508E-5 0.0 0.0 8.447341729069484E-4 0.0021195148338392523 28-29 6.14352125750508E-5 0.0 0.0 0.0028720961878836247 0.002242385258989354 30-31 6.14352125750508E-5 0.0 0.0 0.010121451271739617 0.0023345380778519303 32-33 6.14352125750508E-5 0.0 0.0 0.026509294226134416 0.0024881261092895572 34-35 6.14352125750508E-5 0.0 0.0 0.05096050883100463 0.002641714140727184 36-37 6.14352125750508E-5 0.0 0.0 0.09261358295688907 0.0026877905501584724 38-39 9.215281886257618E-5 0.0 0.0 0.16042269883660137 0.002749225762733523 40-41 9.215281886257618E-5 0.0 0.0 0.23131893414820998 0.002795302172164811 42-43 9.215281886257618E-5 0.0 0.0 0.30637740511177824 0.0028720961878836247 44-45 9.215281886257618E-5 0.0 0.0 0.3998971574541494 0.0029181725973149125 46-47 1.53588031437627E-4 0.0 0.0 0.5100812112075029 0.002994966613033726 48-49 1.53588031437627E-4 0.0 0.0 0.6288815535245074 0.0031485546444713535 50-51 1.53588031437627E-4 0.0 0.0 0.7620270179777863 0.0032867838727652175 52-53 1.53588031437627E-4 0.0 0.0 0.8874777220560399 0.0034403719042028443 54-55 1.53588031437627E-4 0.0 0.0 1.0243246580669656 0.003501807116777895 56-57 1.53588031437627E-4 0.0 0.0 1.1860221375644993 0.0035939599356404712 58-59 1.53588031437627E-4 0.0 0.0 1.355107201374183 0.0036246775419279966 60-61 1.8430563772515237E-4 0.0 0.0 1.543098951853838 0.0036246775419279966 62-63 1.8430563772515237E-4 0.0 0.0 1.7294780280033986 0.0037782655733656235 64-65 1.8430563772515237E-4 0.0 0.0 1.926009273030986 0.0038550595890844376 66-67 1.8430563772515237E-4 0.0 0.0 2.1590483931312976 0.0039011359985157254 68-69 1.8430563772515237E-4 0.0 0.0 2.4164158574113292 0.003931853604803251 70-71 2.1502324401267777E-4 0.0 0.0 2.68067942430291 0.003977930014234538 72-73 2.1502324401267777E-4 0.0 0.0 2.963926471880182 0.004346541289684844 74-75 2.1502324401267777E-4 0.0 0.0 3.2465591673317027 0.004530846927409996 76-77 2.1502324401267777E-4 0.0 0.0 3.5508631340190733 0.004576923336841284 78-79 2.1502324401267777E-4 1.53588031437627E-5 0.0 3.897910649855535 0.004638358549416335 80-81 2.457408503002032E-4 3.07176062875254E-5 0.0 4.259579746284859 0.004684434958847622 82-83 2.457408503002032E-4 3.07176062875254E-5 0.0 4.646145462610223 0.0048994582028603 84-85 2.457408503002032E-4 3.07176062875254E-5 0.0 5.032849408163879 0.004945534612291589 86-87 2.457408503002032E-4 6.14352125750508E-5 0.0 5.438122146718346 0.00523735187202308 88-89 2.457408503002032E-4 6.14352125750508E-5 3.07176062875254E-5 5.855021499252641 0.005529169131754571 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4435 0.0 55.29043 1 GTATCAA 8285 0.0 45.63146 1 TATCAAC 11040 0.0 34.224125 2 ATCAACG 11180 0.0 33.540497 3 TCAACGC 11280 0.0 33.285286 4 CAACGCA 11675 0.0 32.076252 5 AACGCAG 12030 0.0 31.123474 6 ACGCAGA 13740 0.0 27.004639 7 CGCAGAG 13830 0.0 26.828901 8 TAAGGTG 2185 0.0 26.535217 5 TACATGG 11015 0.0 25.413534 2 GTACATG 11425 0.0 25.38034 1 AGGTAAG 2120 0.0 23.763168 2 TAAGGTA 1100 0.0 23.763166 4 GTAAGGT 2360 0.0 23.561783 4 ACATGGG 11320 0.0 23.175385 3 GCAGAGT 15710 0.0 23.043734 9 AAGGTAA 2145 0.0 22.863688 1 TGGTATC 2030 0.0 21.539026 2 GGTAAGG 2540 0.0 21.517828 3 >>END_MODULE