FastQCFastQC Report
Thu 26 May 2016
SRR938382_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938382_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3255462
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82280.252744464533759No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA67200.20642231425217067No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA66400.20396490574916865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65250.2004323810261032No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA62460.19186216887188362No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC59570.1829847806547888No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA57530.1767183889721336No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG52190.16031518721459503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47440.14572432422802048No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA46440.14265256359926795No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA45240.1389664508447649No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA43590.1338980458073232No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG41090.12621864423544185No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA40600.12471348152735309No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT36170.11110558194197936No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT34780.10683583466801333No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT33880.10407125010213603No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG33830.10391766207069843No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG33360.10247393457518472No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49050.057.411421
GTATCAA88550.043.5660171
TATCAAC114450.033.332472
ATCAACG114200.033.3026663
TCAACGC115200.033.0528144
CAACGCA120000.031.691095
AACGCAG125550.030.3388426
ACGCAGA140850.026.9420917
CGCAGAG141800.026.7615918
TAAGGTG25050.026.7571455
TAAGGTA11750.026.7014274
AGGTAAG22350.026.3908332
GTGTAAG13200.025.2254921
AAGGTAA23100.024.9166131
TATATCG1201.7028306E-423.7683165
GTAAGGT28450.023.5594564
GTGGTAT23150.023.2189751
GCAGAGT161900.023.0870939
TGGTATC20150.022.4262922
GGTAAGG29000.022.294413
Produced by FastQC (version 0.11.5)