##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938382_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3255462 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.767799163375276 31.0 31.0 34.0 30.0 34.0 2 32.00087391589888 31.0 31.0 34.0 30.0 34.0 3 32.064306387234744 33.0 31.0 34.0 30.0 34.0 4 35.7418627525064 37.0 35.0 37.0 33.0 37.0 5 35.60113096082829 37.0 35.0 37.0 33.0 37.0 6 35.63141944215598 37.0 35.0 37.0 33.0 37.0 7 35.565140063069386 37.0 35.0 37.0 33.0 37.0 8 35.553766254989306 37.0 35.0 37.0 33.0 37.0 9 37.13355216556052 39.0 37.0 39.0 33.0 39.0 10-11 37.038693432760084 39.0 37.0 39.0 33.0 39.0 12-13 36.924819887315536 39.0 37.0 39.0 33.0 39.0 14-15 37.978839869732774 40.0 37.0 41.0 33.0 41.0 16-17 37.89209196728452 40.0 37.0 41.0 33.0 41.0 18-19 37.991547897041954 40.0 37.0 41.0 33.0 41.0 20-21 38.035822104512356 40.0 37.0 41.0 33.0 41.0 22-23 37.940061349203276 40.0 37.0 41.0 33.0 41.0 24-25 37.820761385020006 40.0 37.0 41.0 32.0 41.0 26-27 37.618997088585274 40.0 37.0 41.0 32.0 41.0 28-29 37.4968913782437 39.5 36.5 41.0 32.0 41.0 30-31 37.24683961907711 39.0 36.0 41.0 31.0 41.0 32-33 37.027155592662425 39.0 36.0 41.0 31.0 41.0 34-35 36.86932638132468 39.0 35.5 40.5 30.0 41.0 36-37 36.85626080107831 39.0 35.5 41.0 30.0 41.0 38-39 36.72220670983105 39.0 35.0 41.0 30.0 41.0 40-41 36.52907068182642 39.0 35.0 40.5 30.0 41.0 42-43 36.2972118857477 39.0 35.0 40.0 30.0 41.0 44-45 36.025243575259054 38.0 35.0 40.0 29.0 41.0 46-47 35.65834480635928 38.0 34.5 40.0 28.0 41.0 48-49 35.27697819848611 38.0 34.0 40.0 27.0 41.0 50-51 34.87679767111396 38.0 34.0 40.0 26.0 41.0 52-53 34.342818008626736 37.5 33.0 40.0 24.0 41.0 54-55 33.99295629929024 37.0 33.0 40.0 24.0 41.0 56-57 33.616280269897175 37.0 32.0 39.5 23.0 41.0 58-59 33.12658986650743 36.0 32.0 39.0 22.0 40.0 60-61 32.71801575321721 35.5 31.0 39.0 21.5 40.0 62-63 32.22633638482034 35.0 31.0 38.5 20.0 40.0 64-65 31.691306948138237 35.0 30.0 38.0 18.0 40.0 66-67 31.17126923920476 34.5 30.0 37.5 17.0 40.0 68-69 30.60351311119589 34.0 29.0 37.0 12.5 39.0 70-71 29.996424009864036 34.0 28.5 36.0 8.0 39.0 72-73 29.540835371446512 34.0 28.0 36.0 7.5 38.5 74-75 29.01682741190037 33.0 27.5 35.0 7.0 37.5 76-77 27.801272599710884 31.5 26.0 34.0 2.0 36.0 78-79 28.50007049690643 33.0 27.0 35.0 2.0 36.0 80-81 28.235493303254653 33.0 27.0 35.0 2.0 36.0 82-83 27.763185225322857 33.0 26.0 35.0 2.0 36.0 84-85 27.252694702011574 32.5 25.5 35.0 2.0 35.0 86-87 26.677334123390168 32.0 25.0 34.5 2.0 35.0 88-89 26.002548332617614 32.0 23.5 34.0 2.0 35.0 90-91 25.440157188134894 31.0 21.5 34.0 2.0 35.0 92-93 24.67788350777862 31.0 18.5 34.0 2.0 35.0 94-95 23.882155743178693 31.0 12.0 34.0 2.0 35.0 96-97 22.83923249603282 30.0 2.0 34.0 2.0 35.0 98-99 21.534208355066042 29.5 2.0 34.0 2.0 35.0 100-101 17.85669161550649 23.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4723171040683951 1101 2 0.44963732587344296 1101 3 0.42560739193746855 1101 4 0.3066967734667472 1101 5 0.24446165219364246 1101 6 0.3289618176851974 1101 7 0.4266137027079111 1101 8 0.35773733397243035 1101 9 0.46448364937209163 1101 10-11 0.4853854900572969 1101 12-13 0.5602513550590729 1101 14-15 0.6978949024183478 1101 16-17 0.6489520647880127 1101 18-19 0.6919780240133235 1101 20-21 0.6546115793167502 1101 22-23 0.6549584260886121 1101 24-25 0.6399852903303227 1101 26-27 0.6287875244980867 1101 28-29 0.6283278925981648 1101 30-31 0.65812601415697 1101 32-33 0.6186245826151762 1101 34-35 0.5011356994810683 1101 36-37 0.6566661522271247 1101 38-39 0.7364771727160431 1101 40-41 0.784528687498792 1101 42-43 0.8605392135741852 1101 44-45 0.9507914681757228 1101 46-47 0.980720490579138 1101 48-49 1.0368524773565895 1101 50-51 1.0435745927056743 1101 52-53 1.08921796734478 1101 54-55 1.0806396465337471 1101 56-57 1.0987789331201654 1101 58-59 1.1961456221125104 1101 60-61 1.2009922186276683 1101 62-63 1.0458762925221805 1101 64-65 1.0759126576436238 1101 66-67 1.004674526283324 1101 68-69 1.0021447550100042 1101 70-71 1.0032124956100965 1101 72-73 0.9823144647977493 1101 74-75 1.0611682825840383 1101 76-77 1.0803044096031336 1101 78-79 1.057723534336219 1101 80-81 1.0664199565910941 1101 82-83 1.0666481619555164 1101 84-85 1.3474946969114292 1101 86-87 1.3280694624961527 1101 88-89 1.419693431097837 1101 90-91 1.4328111790854514 1101 92-93 1.3931524472626293 1101 94-95 1.4789597647473514 1101 96-97 1.4830153479585455 1101 98-99 1.0322731378450491 1101 100-101 0.6611930363347405 1102 1 0.3178622493537375 1102 2 0.3388770099839 1102 3 0.33627645865820455 1102 4 0.28976938184190715 1102 5 0.21848322735950632 1102 6 0.2018066961767957 1102 7 0.259421189637024 1102 8 0.2450381858266013 1102 9 0.3181333232352017 1102 10-11 0.38548857762003763 1102 12-13 0.42492934790993075 1102 14-15 0.46870463533549156 1102 16-17 0.48582726355115113 1102 18-19 0.5400055472871443 1102 20-21 0.45399851490738286 1102 22-23 0.45033192883411743 1102 24-25 0.4141686348343896 1102 26-27 0.3578253474580677 1102 28-29 0.3562672392057422 1102 30-31 0.3956052355553439 1102 32-33 0.4365481944572025 1102 34-35 0.47586960961093894 1102 36-37 0.48446665044116344 1102 38-39 0.6493914716251865 1102 40-41 0.7647008421223873 1102 42-43 0.6678763912519443 1102 44-45 0.7228865124331776 1102 46-47 0.5753336339741182 1102 48-49 0.8144185980462311 1102 50-51 0.688177787314693 1102 52-53 0.6774033257930157 1102 54-55 0.731311080933267 1102 56-57 0.6609357448828987 1102 58-59 0.7490539838734165 1102 60-61 0.8712290318567426 1102 62-63 0.8315768920758941 1102 64-65 0.829276726710308 1102 66-67 0.8952026395872394 1102 68-69 0.813641479469073 1102 70-71 0.8590828839982301 1102 72-73 0.7084364809414261 1102 74-75 0.691188852856385 1102 76-77 0.5049743848473831 1102 78-79 0.7393016801130443 1102 80-81 0.8449635242427469 1102 82-83 0.724330750521915 1102 84-85 0.510453259887246 1102 86-87 0.4392132743229453 1102 88-89 0.7198836390359666 1102 90-91 0.5706817949823808 1102 92-93 0.6014559326634235 1102 94-95 0.5783224850118636 1102 96-97 0.6913125031726501 1102 98-99 0.5554809722352729 1102 100-101 0.0915801622088992 1103 1 0.26542110451449474 1103 2 0.3169269083464705 1103 3 0.2595291222087752 1103 4 0.14983178249427453 1103 5 0.18841524255784492 1103 6 0.14105395876181603 1103 7 0.2159639881526445 1103 8 0.19194334097140597 1103 9 0.365608379224426 1103 10-11 0.39498643259761224 1103 12-13 0.3648741675034941 1103 14-15 0.48162370505758645 1103 16-17 0.4596801862180726 1103 18-19 0.47849726930288483 1103 20-21 0.4297885316893826 1103 22-23 0.4051675362063776 1103 24-25 0.4893247472619251 1103 26-27 0.5162799230627186 1103 28-29 0.48718082375685157 1103 30-31 0.59557585598683 1103 32-33 0.7003192901322848 1103 34-35 0.582834598619911 1103 36-37 0.6462056069734388 1103 38-39 0.5613008702802702 1103 40-41 0.7003867322476012 1103 42-43 0.7986028218578056 1103 44-45 0.7210739721770736 1103 46-47 0.6805076880665055 1103 48-49 0.7569732318560369 1103 50-51 0.8917119961382483 1103 52-53 0.9715265072512764 1103 54-55 0.8434896045610145 1103 56-57 0.7664462513563706 1103 58-59 0.871973038822631 1103 60-61 0.9337679030017583 1103 62-63 0.8429305903190993 1103 64-65 0.7633733317543623 1103 66-67 0.735400130856469 1103 68-69 0.6162264752440301 1103 70-71 0.34748414057954946 1103 72-73 0.32433869406683513 1103 74-75 0.4483091971827129 1103 76-77 0.36967574735026076 1103 78-79 0.4275887977170072 1103 80-81 0.45591100517095384 1103 82-83 0.46024120691532033 1103 84-85 0.38207571747430435 1103 86-87 0.4629363987578827 1103 88-89 0.4527725794657975 1103 90-91 0.45575606933843105 1103 92-93 0.4249223967446376 1103 94-95 0.45599693058561286 1103 96-97 0.30732375863122385 1103 98-99 0.1718575795248043 1103 100-101 0.05245473896583519 1104 1 0.2832018086848933 1104 2 0.26239774196670496 1104 3 0.24088627982084176 1104 4 0.19541465104760647 1104 5 0.1828981317680629 1104 6 0.20099049620816345 1104 7 0.1773578404329328 1104 8 0.23043831446172902 1104 9 0.18328766380749784 1104 10-11 0.3449709399308034 1104 12-13 0.3543825478886262 1104 14-15 0.4556125216473603 1104 16-17 0.4194645225581297 1104 18-19 0.45659343572854993 1104 20-21 0.4096717341957472 1104 22-23 0.37871667665027076 1104 24-25 0.43769184887086965 1104 26-27 0.4592170601326515 1104 28-29 0.5207511375120433 1104 30-31 0.4781439519954631 1104 32-33 0.5286928183289419 1104 34-35 0.45511154725311087 1104 36-37 0.5039990639038194 1104 38-39 0.4498652021446645 1104 40-41 0.44017448271238635 1104 42-43 0.4712653657537018 1104 44-45 0.35357608090117054 1104 46-47 0.33641699284742543 1104 48-49 0.5037710554232504 1104 50-51 0.6413745285580816 1104 52-53 0.6191332488565493 1104 54-55 0.4169954946434089 1104 56-57 0.4632920591554992 1104 58-59 0.5017678757479658 1104 60-61 0.38525254517853824 1104 62-63 0.3742286462763147 1104 64-65 0.3434220045492431 1104 66-67 0.06321420164074354 1104 68-69 0.06306152424675915 1104 70-71 0.013914830978688286 1104 72-73 -0.15854513675324 1104 74-75 -0.07075180375832346 1104 76-77 -0.1894269833757143 1104 78-79 -0.027269085755190048 1104 80-81 0.019311157802412993 1104 82-83 0.07350228417208626 1104 84-85 -0.10030244991535753 1104 86-87 0.17227352779458371 1104 88-89 0.13536800281726613 1104 90-91 0.26116853290197284 1104 92-93 0.19617388863107266 1104 94-95 0.2534367673961917 1104 96-97 0.02947927631581848 1104 98-99 -0.19790037624650125 1104 100-101 -0.1568189131721489 1105 1 0.22303596146448257 1105 2 0.22089242644363694 1105 3 0.214967277576946 1105 4 0.13954727081004137 1105 5 0.05721361961311544 1105 6 0.14165419587851602 1105 7 0.15459541561790502 1105 8 0.1703644089042058 1105 9 0.24043704751346695 1105 10-11 0.19443615024152905 1105 12-13 0.2567570773027157 1105 14-15 0.3362077545627855 1105 16-17 0.26893374722315144 1105 18-19 0.22692678770920338 1105 20-21 0.2481245347129004 1105 22-23 0.25374788501753187 1105 24-25 0.30014271835410966 1105 26-27 0.2883389473246396 1105 28-29 0.28262689246964356 1105 30-31 0.3816886009388867 1105 32-33 0.37646539557547953 1105 34-35 0.33734360075850134 1105 36-37 0.42276782777277333 1105 38-39 0.3830465338950475 1105 40-41 0.312373499376875 1105 42-43 0.3174902151765622 1105 44-45 0.2521311464465086 1105 46-47 -0.038935881844629705 1105 48-49 -0.01906285079729031 1105 50-51 0.08259143214934994 1105 52-53 0.11752634168085052 1105 54-55 0.0826238679959701 1105 56-57 0.08307948485109762 1105 58-59 0.08856158248358525 1105 60-61 0.09078942751185792 1105 62-63 0.21313649327710493 1105 64-65 -0.009601736601052124 1105 66-67 -0.05753304321725494 1105 68-69 -0.03744353332166739 1105 70-71 0.015935475637366636 1105 72-73 0.07695462704732847 1105 74-75 -0.04364299869951438 1105 76-77 0.03598898142642781 1105 78-79 0.1301992707733426 1105 80-81 0.39150331121600956 1105 82-83 0.24473210621446384 1105 84-85 0.2089447576480019 1105 86-87 0.2998829875816327 1105 88-89 0.22343218793960418 1105 90-91 0.28772996132837036 1105 92-93 0.33630632088431156 1105 94-95 0.2786774732595312 1105 96-97 0.34057088543477576 1105 98-99 0.24230801794962176 1105 100-101 -0.0889387719497563 1106 1 0.1438588973142778 1106 2 0.1740465416677317 1106 3 0.2180334649864335 1106 4 0.16598139707635085 1106 5 0.08747152909494815 1106 6 0.06982996225672622 1106 7 0.22633610614053623 1106 8 0.16404185678187133 1106 9 0.09148904193632035 1106 10-11 0.11370212292968063 1106 12-13 0.07363709021297637 1106 14-15 0.19246056635886788 1106 16-17 0.02923666100927136 1106 18-19 0.3294881361052049 1106 20-21 0.27515773245734465 1106 22-23 0.23185090144231424 1106 24-25 0.11635153251430808 1106 26-27 0.10425808486927224 1106 28-29 0.12897854976235834 1106 30-31 0.3126120790923679 1106 32-33 0.3532396356575873 1106 34-35 0.039167642386829016 1106 36-37 -0.19370305745717076 1106 38-39 0.09510227011471528 1106 40-41 0.25303428896813074 1106 42-43 0.3938810391346479 1106 44-45 0.3104347597114909 1106 46-47 -0.06279132841363122 1106 48-49 0.14324413740133934 1106 50-51 0.188624359546246 1106 52-53 0.12955135894728897 1106 54-55 -0.22895777410850116 1106 56-57 0.134682396717281 1106 58-59 0.18644270117238193 1106 60-61 -0.17273819801165047 1106 62-63 -0.027013853167311197 1106 64-65 -0.4085864544407194 1106 66-67 -0.10687652937219383 1106 68-69 -0.7109158491623226 1106 70-71 -0.4906243440755844 1106 72-73 -0.23202355782576234 1106 74-75 0.18802473949820708 1106 76-77 0.21439189682113735 1106 78-79 0.07229988157198619 1106 80-81 -0.3823950744303488 1106 82-83 -0.08722639829141343 1106 84-85 0.01778958490318061 1106 86-87 0.10039179618958016 1106 88-89 -0.014148948755067892 1106 90-91 0.008006909296586429 1106 92-93 -0.12804723973814447 1106 94-95 -0.023691207107166434 1106 96-97 -0.15275721218507599 1106 98-99 -0.4312238418079808 1106 100-101 -0.770056319165878 1107 1 0.17284483738447776 1107 2 0.19306622901150305 1107 3 0.24100982232249635 1107 4 0.09677265852180028 1107 5 0.06780755414497008 1107 6 0.1705229109983648 1107 7 0.10876884570340906 1107 8 0.24036062163942518 1107 9 0.2950938804459682 1107 10-11 0.20778836078781637 1107 12-13 0.15782937526329022 1107 14-15 0.3417345469426678 1107 16-17 0.3307742280301156 1107 18-19 0.3105505349000808 1107 20-21 0.27233617962382084 1107 22-23 0.3449973635297354 1107 24-25 0.3536198711735352 1107 26-27 0.2961092091287867 1107 28-29 0.33382585857716407 1107 30-31 0.2906894698701379 1107 32-33 0.3265985076506297 1107 34-35 0.3603701123612808 1107 36-37 0.3210276924153135 1107 38-39 0.3184482766029859 1107 40-41 0.23821508347759135 1107 42-43 0.3701697029292248 1107 44-45 0.44840000378234635 1107 46-47 0.39171354706430606 1107 48-49 0.3160628353582666 1107 50-51 0.4086044547121688 1107 52-53 0.5737952140025797 1107 54-55 0.3835228875614547 1107 56-57 0.358273058706132 1107 58-59 0.16710723950492223 1107 60-61 0.09880300172155643 1107 62-63 0.2087800564630271 1107 64-65 0.3421429985888871 1107 66-67 0.42788652852460274 1107 68-69 0.39608355910882054 1107 70-71 0.10198202327785211 1107 72-73 0.2326756905419174 1107 74-75 0.19566334822842535 1107 76-77 0.07859704231438869 1107 78-79 0.28628195186708183 1107 80-81 0.20269467448730438 1107 82-83 0.17342088254829946 1107 84-85 0.3503785749669639 1107 86-87 0.44343630779899 1107 88-89 0.32120123172476056 1107 90-91 0.4305898099693195 1107 92-93 0.29776367935495784 1107 94-95 0.10084282961916813 1107 96-97 0.17094205153684783 1107 98-99 0.13948602697062285 1107 100-101 -0.2977187573418547 1108 1 0.16783491441068676 1108 2 0.1192732631233504 1108 3 0.15669632479927742 1108 4 0.1357754272092393 1108 5 0.10769741986723602 1108 6 0.04504960267500735 1108 7 0.1436914652358965 1108 8 0.1204643817458475 1108 9 0.12180011691629034 1108 10-11 0.14568498038046584 1108 12-13 0.14747640179772503 1108 14-15 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66-67 -0.48587946094676226 2316 68-69 -0.42051360262285087 2316 70-71 -0.6240689105248798 2316 72-73 -0.6839075681586237 2316 74-75 -0.4850474094149355 2316 76-77 -0.4543812191093757 2316 78-79 -0.4832809576842827 2316 80-81 -0.5084959164458347 2316 82-83 -0.25744018464556717 2316 84-85 -0.40049452575151534 2316 86-87 -0.25035994419962293 2316 88-89 -0.013927128645310205 2316 90-91 -0.09332861611995114 2316 92-93 -0.23779400171056153 2316 94-95 -0.24523712446140777 2316 96-97 -0.07534326792230672 2316 98-99 -0.1325931384830703 2316 100-101 -0.29195521942954983 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 20.0 4 56.0 5 107.0 6 252.0 7 688.0 8 1562.0 9 2895.0 10 4410.0 11 6962.0 12 9608.0 13 13404.0 14 17197.0 15 22399.0 16 27615.0 17 33912.0 18 33471.0 19 30115.0 20 30784.0 21 31554.0 22 34842.0 23 38713.0 24 44993.0 25 52850.0 26 62625.0 27 74903.0 28 89685.0 29 105999.0 30 125826.0 31 150787.0 32 181282.0 33 223329.0 34 284352.0 35 371130.0 36 471889.0 37 478680.0 38 190277.0 39 6287.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.9438942718395 18.329056905619296 14.763612817057464 23.96343600548374 2 16.537794403460744 19.79819095304419 38.03442548990924 25.629589153585826 3 19.91105914993509 22.684647336044296 29.32698126387301 28.0773122501476 4 14.070015254363282 16.100817641244163 34.357581197384576 35.47158590700798 5 14.581700670630457 36.623039152238 33.49951777202992 15.295742405101626 6 35.0184397790544 34.578287198560446 15.857749222690973 14.545523799694177 7 30.446154800762532 31.745018065024254 19.118300259686645 18.690526874526565 8 27.999651047992575 34.17385919417889 18.531716850020057 19.29477290780848 9 26.83889414159956 16.247463493660806 18.76756663109568 38.14607573364395 10-11 26.110103573624883 25.588349672028116 26.302150048134486 21.99939670621251 12-13 26.96700191862169 22.560791678723326 26.706977381397785 23.7652290212572 14-15 23.116273512023795 24.233088882622496 24.613603230509217 28.037034374844495 16-17 23.423372780883327 27.398768592599144 25.77105799422632 23.40680063229121 18-19 23.629426483860048 25.665420146203523 27.712748605267084 22.992404764669345 20-21 23.6054053157432 24.950513936270795 28.165387892716915 23.278692855269085 22-23 23.56805270649757 24.834416743307095 28.24078425734965 23.356746292845685 24-25 23.64929828570867 25.253727895569778 27.911466166337373 23.18550765238418 26-27 22.908750080065076 25.658503587145905 27.752637385913985 23.680108946875038 28-29 23.207074756209717 26.148577375500004 27.522560545937875 23.121787322352404 30-31 23.217580177560052 25.491481700600403 27.438870427607508 23.852067694232034 32-33 22.789561051549672 25.4775819837553 27.515157602822583 24.217699361872448 34-35 23.777509652138985 25.5814439308072 27.274828865098925 23.36621755195489 36-37 24.04063820810494 24.995542102594765 27.303849826376226 23.659969862924072 38-39 23.317979484956695 25.408604411519182 27.321427995448065 23.951988108076062 40-41 23.731789423692636 25.230014387370943 27.580914177880867 23.457282011055558 42-43 23.58346323094959 25.333437159273135 27.115050353276978 23.968049256500297 44-45 23.352184728311986 25.53591471809531 27.61953602898759 23.49236452460511 46-47 24.02296509681268 25.949542645559987 26.66510928402789 23.362382973599445 48-49 23.608031671080788 26.06666887833432 26.437660768271908 23.887638682312986 50-51 23.830872545893637 26.119963925243177 26.651593537261377 23.397569991601806 52-53 24.295860311071056 25.51634760289016 26.736650589071537 23.45114149696725 54-55 23.47439472492691 25.958727209839953 26.90155805842612 23.665320006807022 56-57 23.570371885772282 26.12845734338168 26.64090381027332 23.660266960572724 58-59 23.51105618803107 25.60650377734405 26.678072113881225 24.20436792074366 60-61 22.89289507910091 26.005295100971843 27.108103243103436 23.99370657682381 62-63 23.281119546165797 26.0108242701036 27.19469617522797 23.513360008502634 64-65 22.859044276972053 26.150650813924415 26.91888278837228 24.07142212073125 66-67 22.763929331635058 26.194627824428473 26.928226261146854 24.11321658278962 68-69 23.62015518996802 25.5886051287549 27.09057284146378 23.7006668398133 70-71 23.254805272556677 25.914782255843683 26.501196988874536 24.3292154827251 72-73 23.149387091601746 26.0859288174766 26.58200280021699 24.182681290704668 74-75 23.163563266903438 26.542069912043203 26.387990398904982 23.906376422148377 76-77 23.335643297326154 26.030529614537045 26.63821601972316 23.995611068413638 78-79 23.33917582204922 26.338673282010355 26.65222324819027 23.66992764775015 80-81 23.71277539995168 26.27813976333962 26.29388271790668 23.71520211880202 82-83 23.410228618549635 26.394552264675376 26.416423317921716 23.778795798853274 84-85 23.1420417254874 26.71277198525912 26.34231463424037 23.802871655013107 86-87 23.70462001977338 26.62559422667631 25.870415079745523 23.799370673804784 88-89 23.210659387815973 26.863769440806966 25.597312084102423 24.32825908727464 90-91 23.03580871769738 26.83172539866095 25.936572733054785 24.195893150586883 92-93 23.287278001813586 26.353399487379864 26.2068290782913 24.152493432515254 94-95 22.930062111038072 25.98003047188416 26.52798682828233 24.561920588795438 96-97 23.2510777272166 25.513828759174583 26.49047969228331 24.744613821325515 98-99 22.870081126821308 26.13865297315263 25.948572221086526 25.042693678939543 100-101 22.979288346784575 25.654269655121148 26.46624350092245 24.90019849717183 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 444.0 1 317.5 2 252.0 3 337.5 4 329.5 5 314.0 6 556.0 7 1241.5 8 1999.0 9 2306.5 10 1986.5 11 1651.0 12 1741.0 13 1907.0 14 2073.5 15 2238.5 16 2476.0 17 2858.0 18 3197.5 19 3571.0 20 4042.5 21 4754.5 22 5870.5 23 7119.0 24 8604.0 25 10791.0 26 13968.0 27 18017.5 28 21350.5 29 24377.5 30 28207.0 31 35265.5 32 48439.5 33 51417.0 34 45982.5 35 47388.5 36 52139.5 37 57711.5 38 63905.5 39 69152.0 40 74288.0 41 81992.5 42 86283.0 43 89559.0 44 95787.5 45 105022.0 46 110896.0 47 115770.5 48 119831.5 49 120050.0 50 122194.5 51 123497.5 52 125767.0 53 132941.0 54 140657.0 55 135512.0 56 124837.5 57 116313.0 58 109754.0 59 98560.5 60 79310.0 61 66297.0 62 58024.5 63 44372.0 64 30775.0 65 23354.5 66 18091.0 67 14263.5 68 10937.5 69 8247.5 70 6319.5 71 4341.5 72 3070.5 73 2359.5 74 1776.0 75 1349.0 76 1029.5 77 727.0 78 469.0 79 278.0 80 178.0 81 126.0 82 81.0 83 43.0 84 20.0 85 12.0 86 7.5 87 4.5 88 3.5 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.836345194629826E-4 2 0.04896386442231548 3 0.0012287042515010157 4 0.0 5 0.0875758955257349 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.8430563772515237E-4 26-27 0.009138487870538805 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010889391428927753 36-37 1.0751162200633889E-4 38-39 0.004407976502259894 40-41 0.005421657509748232 42-43 4.146876848815928E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 7.679401571881348E-5 68-69 9.983222043445754E-4 70-71 4.607640943128809E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0011519102357822023 82-83 5.375581100316944E-4 84-85 0.004960893415435351 86-87 0.002319179274708167 88-89 9.215281886257618E-5 90-91 0.0029335314004586754 92-93 9.215281886257619E-4 94-95 6.14352125750508E-5 96-97 0.0 98-99 1.0751162200633889E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3255462.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.86342798503064 #Duplication Level Percentage of deduplicated Percentage of total 1 69.22748683289193 28.98099909613428 2 15.146549607073714 12.681729773948492 3 5.850676525734128 7.347881261963398 4 2.943173309679126 4.9284529558886545 5 1.7160579512298515 3.5920034229725055 6 1.1117023306442855 2.7923802275830667 7 0.7328966405483512 2.1477096012446752 8 0.5166695968795624 1.7303648368817894 9 0.3781384035954594 1.424715284456367 >10 2.1414147539330504 16.327425757316693 >50 0.1466563803004404 4.244901054794198 >100 0.07590989270138394 5.782157447781406 >500 0.007364985342689071 2.051307300693102 >1k 0.004713590618627677 4.38664223993626 >5k 5.891988273284596E-4 1.5813297384051264 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8228 0.252744464533759 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6720 0.20642231425217067 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6640 0.20396490574916865 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6525 0.2004323810261032 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6246 0.19186216887188362 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5957 0.1829847806547888 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5753 0.1767183889721336 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5219 0.16031518721459503 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4744 0.14572432422802048 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4644 0.14265256359926795 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4524 0.1389664508447649 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4359 0.1338980458073232 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4109 0.12621864423544185 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 4060 0.12471348152735309 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3617 0.11110558194197936 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3478 0.10683583466801333 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3388 0.10407125010213603 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3383 0.10391766207069843 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3336 0.10247393457518472 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.215281886257618E-5 2 0.0 0.0 0.0 0.0 9.215281886257618E-5 3 0.0 0.0 0.0 0.0 9.215281886257618E-5 4 0.0 0.0 0.0 0.0 9.215281886257618E-5 5 0.0 0.0 0.0 0.0 9.215281886257618E-5 6 0.0 0.0 0.0 0.0 2.1502324401267777E-4 7 0.0 0.0 0.0 0.0 2.457408503002032E-4 8 0.0 0.0 0.0 0.0 3.3789366916277936E-4 9 0.0 0.0 0.0 0.0 3.3789366916277936E-4 10-11 0.0 0.0 0.0 0.0 3.3789366916277936E-4 12-13 1.53588031437627E-5 0.0 0.0 0.0 8.908105823382365E-4 14-15 3.07176062875254E-5 0.0 0.0 0.0 0.0010290398106321008 16-17 4.607640943128809E-5 0.0 0.0 7.67940157188135E-5 0.001228704251501016 18-19 6.14352125750508E-5 0.0 0.0 1.382292282938643E-4 0.0013362158735073547 20-21 6.14352125750508E-5 0.0 0.0 1.9966444086891508E-4 0.0015358803143762697 22-23 6.14352125750508E-5 0.0 0.0 3.07176062875254E-4 0.001781621164676473 24-25 6.14352125750508E-5 0.0 0.0 5.06840503744169E-4 0.001827697574107761 26-27 6.14352125750508E-5 0.0 0.0 9.061693854819991E-4 0.0018737739835390493 28-29 6.14352125750508E-5 0.0 0.0 0.0029335314004586754 0.0019044915898265746 30-31 6.14352125750508E-5 0.0 0.0 0.010259680500033482 0.0019505679992578627 32-33 7.67940157188135E-5 0.0 0.0 0.02698541712359106 0.002042720818120439 34-35 1.0751162200633889E-4 0.0 0.0 0.05169773138190524 0.0022116676527018284 36-37 1.228704251501016E-4 0.0 0.0 0.09378085199581504 0.002242385258989354 38-39 1.53588031437627E-4 0.0 0.0 0.16215824359184655 0.002319179274708167 40-41 1.53588031437627E-4 0.0 0.0 0.23300840249402388 0.00233453807785193 42-43 1.53588031437627E-4 0.0 0.0 0.30823582029217356 0.002349896880995693 44-45 1.8430563772515237E-4 0.0 0.0 0.40224705433514507 0.002442049699858269 46-47 1.8430563772515237E-4 0.0 0.0 0.5131529718362555 0.002595637731295896 48-49 1.8430563772515237E-4 0.0 0.0 0.6319686729564038 0.002703149353302235 50-51 1.8430563772515237E-4 0.0 0.0 0.7655134662914205 0.0027645845658772856 52-53 1.8430563772515237E-4 0.0 0.0 0.891424934463987 0.0029181725973149125 54-55 1.8430563772515237E-4 0.0 0.0 1.0290398106321008 0.002948890203602438 56-57 1.8430563772515237E-4 0.0 0.0 1.1910598249956534 0.002994966613033726 58-59 1.8430563772515237E-4 0.0 0.0 1.3608206761436628 0.003041043022465014 60-61 1.8430563772515237E-4 0.0 0.0 1.5491042438830496 0.0031331958413275906 62-63 1.8430563772515237E-4 0.0 0.0 1.7352836555917408 0.003317501479052743 64-65 1.8430563772515237E-4 0.0 0.0 1.9323831763356476 0.003409654297915319 66-67 1.8430563772515237E-4 0.0 0.0 2.1665742066717413 0.0034250131010590814 68-69 1.8430563772515237E-4 0.0 0.0 2.424279564620935 0.0034710895104903697 70-71 1.8430563772515237E-4 0.0 0.0 2.6905551347243493 0.003486448313634132 72-73 1.8430563772515237E-4 0.0 0.0 2.9765053316549235 0.0037014715576468103 74-75 1.8430563772515237E-4 0.0 0.0 3.262655193026366 0.0038704183922282 76-77 1.8430563772515237E-4 0.0 0.0 3.5724115348297723 0.003931853604803251 78-79 1.8430563772515237E-4 0.0 0.0 3.9250650138137075 0.004054724029953352 80-81 1.8430563772515237E-4 0.0 0.0 4.292754761075386 0.004085441636240878 82-83 1.8430563772515237E-4 0.0 0.0 4.687675666310957 0.004285106077109793 84-85 1.9966444086891506E-4 0.0 0.0 5.083625611357159 0.004331182486541081 86-87 2.1502324401267777E-4 0.0 0.0 5.497576073687851 0.004500129321122471 88-89 2.1502324401267777E-4 0.0 0.0 5.927315385650331 0.00466907615570386 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4905 0.0 57.41142 1 GTATCAA 8855 0.0 43.566017 1 TATCAAC 11445 0.0 33.33247 2 ATCAACG 11420 0.0 33.302666 3 TCAACGC 11520 0.0 33.052814 4 CAACGCA 12000 0.0 31.69109 5 AACGCAG 12555 0.0 30.338842 6 ACGCAGA 14085 0.0 26.942091 7 CGCAGAG 14180 0.0 26.761591 8 TAAGGTG 2505 0.0 26.757145 5 TAAGGTA 1175 0.0 26.701427 4 AGGTAAG 2235 0.0 26.390833 2 GTGTAAG 1320 0.0 25.225492 1 AAGGTAA 2310 0.0 24.916613 1 TATATCG 120 1.7028306E-4 23.768316 5 GTAAGGT 2845 0.0 23.559456 4 GTGGTAT 2315 0.0 23.218975 1 GCAGAGT 16190 0.0 23.087093 9 TGGTATC 2015 0.0 22.426292 2 GGTAAGG 2900 0.0 22.29441 3 >>END_MODULE