FastQCFastQC Report
Thu 26 May 2016
SRR938381_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938381_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3268797
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82580.25263116675645503No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA68840.2105973543171999No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA65360.1999512358828034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63310.19367981554070196No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA60110.18389028134815347No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA59700.18263599727973318No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA51240.15675491625818305No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG49930.1527473256981085No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC49080.1501469806782128No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA45610.139531454538168No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA45030.1377571014657686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43800.13399424926050776No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG40120.12273628493907698No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT37280.11404807334319017No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA35220.10774606070673706No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG33460.10236181690083537No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT32890.10061805612278768No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43950.057.4869581
GTATCAA81500.044.4537471
TATCAAC107250.033.3237762
ATCAACG108200.032.9877783
TCAACGC108250.032.9286354
CAACGCA112650.031.5159025
AACGCAG117250.030.3964086
GTGTAAG13100.028.0202351
TAAGGTG24100.027.2146265
ACGCAGA133750.026.498377
CGCAGAG133700.026.4727468
AGGTAAG21200.026.0049362
TACATGG107800.025.7470682
GTACATG113050.025.1320571
GTAAGGT26850.024.2502754
TAAGGTA13400.023.7634964
ACATGGG110650.023.7098063
AAGGTAA22150.023.4587651
TGGTATC19150.023.3288212
GTGGTAT21100.023.2705761
Produced by FastQC (version 0.11.5)