FastQCFastQC Report
Thu 26 May 2016
SRR938381_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938381_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3268797
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82260.2516522133372002No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA66860.20454008003556048No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA66750.20420356479769164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66230.2026127654914025No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA62700.19181368558524742No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC58360.1785366298366035No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA56230.1720204711396884No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG53020.16220034465278815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48070.1470571589486897No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA47170.14430385245703542No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA44440.13595215609901748No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG43050.1316998271841292No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA42790.13090442753098464No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA41680.12750868285794437No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT36200.11074410555320506No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT33580.10272892443305595No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT33440.10230063231213196No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44600.054.4950141
GTATCAA81950.042.774931
AGGTAAG21050.032.0853652
TATCAAC110950.031.2943782
ATCAACG110600.031.165023
TCAACGC111250.031.0684014
TAAGGTG23250.030.8772415
CAACGCA115600.029.9404345
AAGGTAA22800.028.3710081
AACGCAG122100.028.240576
GGTAAGG26400.027.3731123
TGGTATC20250.027.0111792
GTAAGGT26150.026.9075744
GTGGTAT22150.026.6267811
TACATGG106700.025.0074372
GTACATG110650.024.8454671
ACGCAGA138350.024.7862347
CGCAGAG139800.024.529158
ACATGGG107550.023.384593
GTGTAAG11350.021.7910541