##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938381_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3268797 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.775716876881617 31.0 31.0 34.0 30.0 34.0 2 32.01789863365636 31.0 31.0 34.0 30.0 34.0 3 32.08201304638985 33.0 31.0 34.0 30.0 34.0 4 35.76075051463887 37.0 35.0 37.0 35.0 37.0 5 35.6228731854563 37.0 35.0 37.0 33.0 37.0 6 35.66203438145593 37.0 35.0 37.0 33.0 37.0 7 35.59434036436034 37.0 35.0 37.0 33.0 37.0 8 35.571643635257864 37.0 35.0 37.0 33.0 37.0 9 37.14305660461632 39.0 37.0 39.0 33.0 39.0 10-11 37.07247452197245 39.0 37.0 39.0 33.0 39.0 12-13 36.95812740895198 39.0 37.0 39.0 33.0 39.0 14-15 38.00721779296787 40.0 37.0 41.0 33.0 41.0 16-17 37.916754237109245 40.0 37.0 41.0 33.0 41.0 18-19 37.90399679148017 40.0 37.0 41.0 33.0 41.0 20-21 37.8820913320711 40.0 37.0 41.0 32.5 41.0 22-23 37.91738199098934 40.0 37.0 41.0 32.5 41.0 24-25 37.8218278773506 40.0 37.0 41.0 32.0 41.0 26-27 37.63764360405372 40.0 37.0 41.0 32.0 41.0 28-29 37.50280225416262 39.5 36.5 41.0 32.0 41.0 30-31 37.24483686200152 39.0 36.0 41.0 31.0 41.0 32-33 37.0169678631007 39.0 36.0 40.5 31.0 41.0 34-35 36.84067640174658 39.0 35.0 40.0 30.0 41.0 36-37 36.839856528257954 39.0 35.0 41.0 30.0 41.0 38-39 36.670239540724 39.0 35.0 40.5 30.0 41.0 40-41 36.479551192686486 39.0 35.0 40.0 30.0 41.0 42-43 36.23278900463993 38.5 35.0 40.0 30.0 41.0 44-45 35.94310016804347 38.0 35.0 40.0 29.0 41.0 46-47 35.57535539833155 38.0 34.5 40.0 27.5 41.0 48-49 35.15210626416997 38.0 34.0 40.0 26.5 41.0 50-51 34.74139385223371 38.0 33.5 40.0 26.0 41.0 52-53 34.18008980062084 37.0 33.0 40.0 24.0 41.0 54-55 33.812191304629806 37.0 32.5 40.0 23.5 41.0 56-57 33.4051681704309 36.5 32.0 39.0 23.0 41.0 58-59 32.89146894102019 36.0 31.0 39.0 21.5 40.0 60-61 32.434193680427384 35.5 31.0 39.0 20.0 40.0 62-63 31.913327441257444 35.0 30.5 38.5 18.5 40.0 64-65 31.375819146921636 35.0 30.0 38.0 16.5 40.0 66-67 30.843413647283697 34.0 29.0 37.5 12.5 39.5 68-69 30.23770411561195 34.0 29.0 37.0 8.0 39.0 70-71 29.632488649493986 34.0 28.0 36.0 7.0 39.0 72-73 29.034510861335225 33.5 27.5 35.5 4.0 38.5 74-75 28.40483165519303 33.0 26.0 35.0 2.0 37.5 76-77 27.175701794880503 31.5 25.5 34.0 2.0 36.0 78-79 27.791667087310714 32.5 26.0 35.0 2.0 36.0 80-81 27.491397905712716 33.0 26.0 35.0 2.0 36.0 82-83 26.98673120417083 32.0 25.0 35.0 2.0 36.0 84-85 26.397316811047 32.0 24.5 35.0 2.0 35.0 86-87 25.75087960494335 31.5 22.0 34.0 2.0 35.0 88-89 24.958148058750666 31.0 19.5 34.0 2.0 35.0 90-91 24.38879058564971 31.0 17.5 34.0 2.0 35.0 92-93 23.595028385060314 30.5 8.5 34.0 2.0 35.0 94-95 22.77271118396156 30.0 2.0 34.0 2.0 35.0 96-97 21.75242711615313 29.5 2.0 34.0 2.0 35.0 98-99 20.530936763586112 29.0 2.0 34.0 2.0 35.0 100-101 17.43009966663577 22.5 2.0 31.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2875208780677596 1101 2 0.36480355820518895 1101 3 0.3206559152063164 1101 4 0.279565217221446 1101 5 0.21187729709652103 1101 6 0.22670311113583352 1101 7 0.24237310833625259 1101 8 0.35999931301486043 1101 9 0.35912961067448634 1101 10-11 0.4055103760211125 1101 12-13 0.43925754692115504 1101 14-15 0.6095985185644821 1101 16-17 0.6070099006803815 1101 18-19 0.6904502278297358 1101 20-21 0.6443554486706589 1101 22-23 0.5251226048394528 1101 24-25 0.5774322092781077 1101 26-27 0.5356667430513298 1101 28-29 0.5935802059041393 1101 30-31 0.6291329805680661 1101 32-33 0.5572644868504 1101 34-35 0.4719703459490816 1101 36-37 0.5810242878323848 1101 38-39 0.7649370946186522 1101 40-41 0.9151396810794026 1101 42-43 0.7973677766103577 1101 44-45 0.8369315280857634 1101 46-47 0.8320862147404569 1101 48-49 1.0426785472654956 1101 50-51 1.1251933360848199 1101 52-53 0.9406109820585371 1101 54-55 1.0845982688262126 1101 56-57 1.2915770314808839 1101 58-59 1.322976079598888 1101 60-61 1.347645984396685 1101 62-63 1.5253880885641635 1101 64-65 1.6024315298226846 1101 66-67 1.600325420530293 1101 68-69 1.7327009460294498 1101 70-71 1.9251704343177352 1101 72-73 2.040449700611507 1101 74-75 2.0199443004028232 1101 76-77 1.8341309880926282 1101 78-79 2.228324189168685 1101 80-81 2.547118331369667 1101 82-83 2.6896342751368145 1101 84-85 2.9122571343811643 1101 86-87 2.994009026945708 1101 88-89 3.2971062818308816 1101 90-91 3.530067003015958 1101 92-93 3.816850602235924 1101 94-95 3.9220437610899204 1101 96-97 4.206974586019172 1101 98-99 4.50644011910029 1101 100-101 4.946207698798574 1102 1 0.33300685477266967 1102 2 0.3569576896593958 1102 3 0.3349500790027733 1102 4 0.2043643967039941 1102 5 0.12496714997412539 1102 6 0.2103662242988662 1102 7 0.31295964834637147 1102 8 0.316139867127859 1102 9 0.3831693029239247 1102 10-11 0.4231516897155245 1102 12-13 0.4610982203633043 1102 14-15 0.6066520207857735 1102 16-17 0.565454534117606 1102 18-19 0.5500970059895778 1102 20-21 0.6119527320722256 1102 22-23 0.5780456511894627 1102 24-25 0.49619528297869664 1102 26-27 0.5474841911026473 1102 28-29 0.605790630205469 1102 30-31 0.5224105202212215 1102 32-33 0.48673892997315704 1102 34-35 0.5118985695080625 1102 36-37 0.6651387232698553 1102 38-39 0.746701630972602 1102 40-41 0.7461577273776072 1102 42-43 0.7273330515430416 1102 44-45 0.753386896438009 1102 46-47 0.6952080425580007 1102 48-49 0.7840274659097588 1102 50-51 0.8974815174123947 1102 52-53 1.0007231627682742 1102 54-55 0.9717901156403528 1102 56-57 1.1957223305054399 1102 58-59 1.1494695541682134 1102 60-61 1.2937943933255553 1102 62-63 1.4654346052735434 1102 64-65 1.4284371393290805 1102 66-67 1.3872780910264133 1102 68-69 1.4517759990777073 1102 70-71 1.7417421650802645 1102 72-73 1.7632688825616292 1102 74-75 1.8135269440852078 1102 76-77 1.806969289079003 1102 78-79 2.2003990472592854 1102 80-81 2.4453856569367147 1102 82-83 2.452940700545728 1102 84-85 2.506140314244206 1102 86-87 2.6097072626259745 1102 88-89 2.9074725196848803 1102 90-91 3.0487966890399427 1102 92-93 3.2937154852754595 1102 94-95 3.456184285064534 1102 96-97 3.887007531038286 1102 98-99 4.287579896208118 1102 100-101 4.877913182759773 1103 1 0.3487034239111111 1103 2 0.33973516120229164 1103 3 0.3143232495525581 1103 4 0.22735420726891675 1103 5 0.28773068631389975 1103 6 0.2602819035854438 1103 7 0.28374429488157915 1103 8 0.273710812047419 1103 9 0.36806743586139845 1103 10-11 0.3800598938389683 1103 12-13 0.4663530290089568 1103 14-15 0.6149003511992674 1103 16-17 0.543526782506234 1103 18-19 0.5798686760490881 1103 20-21 0.6002494354688892 1103 22-23 0.5271427737337504 1103 24-25 0.4839688704930438 1103 26-27 0.46940120933302154 1103 28-29 0.4480241194457051 1103 30-31 0.6618777079116782 1103 32-33 0.6022993307103022 1103 34-35 0.6233167038281451 1103 36-37 0.8135558224553918 1103 38-39 0.8869924692132756 1103 40-41 0.7773184606676438 1103 42-43 0.856538581235398 1103 44-45 0.9426856246233228 1103 46-47 0.9933122363992126 1103 48-49 1.0537002069423806 1103 50-51 1.0432218205303556 1103 52-53 1.199633633636239 1103 54-55 1.1582409503881266 1103 56-57 1.1714924505919626 1103 58-59 1.432336076389042 1103 60-61 1.3657978243690394 1103 62-63 1.5108909285993661 1103 64-65 1.5215843303668883 1103 66-67 1.6487121434962795 1103 68-69 1.7421825395270893 1103 70-71 1.7389562159855991 1103 72-73 1.918972492070317 1103 74-75 2.2054220589414797 1103 76-77 2.0607538974106525 1103 78-79 2.2309785406800877 1103 80-81 2.4371155868786616 1103 82-83 2.6066658204776196 1103 84-85 2.725906744958408 1103 86-87 2.8630352474609815 1103 88-89 3.054836201198178 1103 90-91 3.2074109549767407 1103 92-93 3.4079394891176413 1103 94-95 3.4743035966000058 1103 96-97 3.9755762073941057 1103 98-99 4.364451825142218 1103 100-101 4.443942577669969 1104 1 0.2881084517825272 1104 2 0.2696413457925928 1104 3 0.23842680343362588 1104 4 0.14918125025479156 1104 5 0.1337051724002265 1104 6 0.16162140608615516 1104 7 0.21548360349417806 1104 8 0.2950484637979187 1104 9 0.20375509972991068 1104 10-11 0.30116188339544436 1104 12-13 0.39895007221515755 1104 14-15 0.5141423367898525 1104 16-17 0.4638548059490688 1104 18-19 0.5398190583171427 1104 20-21 0.4172316991126621 1104 22-23 0.43213671473006343 1104 24-25 0.4881307230058809 1104 26-27 0.5159734877387265 1104 28-29 0.4455711262978852 1104 30-31 0.40847915766601517 1104 32-33 0.47266961294970855 1104 34-35 0.5913501495284521 1104 36-37 0.6227819444727061 1104 38-39 0.6001688413021995 1104 40-41 0.7346361507551151 1104 42-43 0.8042246587465769 1104 44-45 0.7790192443822832 1104 46-47 0.7381315899586056 1104 48-49 0.8972357567694331 1104 50-51 0.8792151225772002 1104 52-53 1.0837099109843749 1104 54-55 0.9675808054126875 1104 56-57 1.1960994966624199 1104 58-59 1.3965813448579851 1104 60-61 1.2452160718848475 1104 62-63 1.350597364362912 1104 64-65 1.3093933065054308 1104 66-67 1.499860489939092 1104 68-69 1.4939125943886147 1104 70-71 1.5671577274316064 1104 72-73 1.84424611667648 1104 74-75 1.8191191968884688 1104 76-77 1.9219016710022103 1104 78-79 2.2224710825973766 1104 80-81 2.446612975998896 1104 82-83 2.5448360454319037 1104 84-85 2.6570074566159363 1104 86-87 2.85740552970039 1104 88-89 3.0145992611052 1104 90-91 3.1927980481972646 1104 92-93 3.4516855838143634 1104 94-95 3.7133076285915436 1104 96-97 4.136669780443654 1104 98-99 4.444886046395002 1104 100-101 4.718705571478768 1105 1 0.25059426226833637 1105 2 0.23030320245445068 1105 3 0.19720698621380706 1105 4 0.1668397399132786 1105 5 0.1336820843771349 1105 6 0.11687296933772018 1105 7 0.21241289642347283 1105 8 0.23300709775655548 1105 9 0.2855482695230762 1105 10-11 0.2428877131212701 1105 12-13 0.35381106307615084 1105 14-15 0.432837863485382 1105 16-17 0.4773824634767223 1105 18-19 0.4350302175283005 1105 20-21 0.33848807236903156 1105 22-23 0.3406408032341517 1105 24-25 0.2707435176186763 1105 26-27 0.3867325064977507 1105 28-29 0.45010599883276114 1105 30-31 0.4977047468915998 1105 32-33 0.43638678466687963 1105 34-35 0.39715678733508497 1105 36-37 0.44941397910474024 1105 38-39 0.5621159312492878 1105 40-41 0.5720695321885003 1105 42-43 0.6119803105022328 1105 44-45 0.5859418033048414 1105 46-47 0.8056294254564378 1105 48-49 0.8498495133831838 1105 50-51 0.7725407599028316 1105 52-53 1.0228441101185766 1105 54-55 1.0572700791019685 1105 56-57 1.0339638545267817 1105 58-59 1.291482739759381 1105 60-61 1.2669803816491552 1105 62-63 1.321568023333569 1105 64-65 1.3912057163178453 1105 66-67 1.4780038280824321 1105 68-69 1.5592690157450377 1105 70-71 1.5292337255076056 1105 72-73 1.6033379877683522 1105 74-75 1.790969124738396 1105 76-77 1.5738073949079343 1105 78-79 1.9785441946704871 1105 80-81 2.3652103785962986 1105 82-83 2.394742731338585 1105 84-85 2.5887746523831296 1105 86-87 2.6645108688057277 1105 88-89 2.8740643885703285 1105 90-91 3.060240087639304 1105 92-93 3.3589159830447635 1105 94-95 3.5223061855901 1105 96-97 3.7649680007418738 1105 98-99 4.311525777034731 1105 100-101 4.62632654309974 1106 1 0.06804359068502919 1106 2 0.1357657754265773 1106 3 0.10189998670215772 1106 4 0.09018831789284576 1106 5 0.037360698552660665 1106 6 0.032720146108154324 1106 7 0.10199201605257713 1106 8 0.06437347713822561 1106 9 0.1344119217974935 1106 10-11 0.20780398212458095 1106 12-13 0.10544302091216196 1106 14-15 0.022523139821643667 1106 16-17 0.05361829599011969 1106 18-19 0.08354336598264211 1106 20-21 0.31781645087413324 1106 22-23 0.1602105927833577 1106 24-25 0.06677944288129822 1106 26-27 0.07629456977342386 1106 28-29 0.24445559382922255 1106 30-31 0.3594195128992226 1106 32-33 0.31370747763003237 1106 34-35 0.351878490865964 1106 36-37 0.41100201046636897 1106 38-39 0.3256607336650106 1106 40-41 0.302999616838072 1106 42-43 0.42293715071517113 1106 44-45 -0.006923931372121217 1106 46-47 -0.23623908533297566 1106 48-49 0.26798936954126873 1106 50-51 0.09436330339547538 1106 52-53 0.3908339594668533 1106 54-55 0.47875433825161906 1106 56-57 0.41161192764676713 1106 58-59 0.708279429299381 1106 60-61 0.7501288317485049 1106 62-63 0.9551801559165938 1106 64-65 0.49749314642179243 1106 66-67 0.8109811842882486 1106 68-69 0.5890707504864956 1106 70-71 0.9270009979293903 1106 72-73 1.2622231386852576 1106 74-75 1.498184047095922 1106 76-77 1.4850765106463228 1106 78-79 1.7684269343621075 1106 80-81 1.6074069908311373 1106 82-83 2.047851803324459 1106 84-85 2.1282574375583607 1106 86-87 2.412726252363658 1106 88-89 2.7170240816917364 1106 90-91 2.661555124002856 1106 92-93 2.3619027966167003 1106 94-95 2.8710739064836694 1106 96-97 3.433109999551327 1106 98-99 3.7373999257551027 1106 100-101 3.8986545189560893 1107 1 0.17861588902414027 1107 2 0.150711626660339 1107 3 0.13044351058957204 1107 4 0.1921004847942811 1107 5 0.034337828070853504 1107 6 0.07701142441288766 1107 7 0.06228503553611375 1107 8 0.13753885292121737 1107 9 0.16741302923593793 1107 10-11 0.1928639675279058 1107 12-13 0.16238322228122115 1107 14-15 0.3355087860930155 1107 16-17 0.3353344973781276 1107 18-19 0.27417711142202705 1107 20-21 0.29307553834890854 1107 22-23 0.26548965339946307 1107 24-25 0.2308988685968103 1107 26-27 0.3432324060356251 1107 28-29 0.24774331229286162 1107 30-31 0.32966357568587057 1107 32-33 0.40936018960231024 1107 34-35 0.38579497755555536 1107 36-37 0.3695607451375835 1107 38-39 0.4757020638227587 1107 40-41 0.6942428932859102 1107 42-43 0.7268519016396482 1107 44-45 0.7305908251690525 1107 46-47 0.7394449769935107 1107 48-49 0.6676198934953348 1107 50-51 0.7639092367143476 1107 52-53 0.7177324145005528 1107 54-55 0.8779123060733056 1107 56-57 0.7838798316579414 1107 58-59 0.8032833863701541 1107 60-61 0.9577839873261809 1107 62-63 1.1092367540402748 1107 64-65 1.2365972446207572 1107 66-67 1.5128093882044489 1107 68-69 1.538292980528496 1107 70-71 1.375099252385791 1107 72-73 1.5705841382423493 1107 74-75 1.5809525941522473 1107 76-77 1.575594250808713 1107 78-79 1.706132464347366 1107 80-81 1.9763100735567534 1107 82-83 2.0168188268324023 1107 84-85 2.318696839900255 1107 86-87 2.4659821823023584 1107 88-89 2.6307487731028125 1107 90-91 2.7324216683651876 1107 92-93 2.822569699230126 1107 94-95 3.100283974327386 1107 96-97 3.3958482316221072 1107 98-99 3.666911916914543 1107 100-101 3.8690014841924025 1108 1 0.1410080291408562 1108 2 0.16465621809518893 1108 3 0.1247990900634548 1108 4 0.006456371884034695 1108 5 -0.015955713550404482 1108 6 -0.009281939915780413 1108 7 0.06229603363055247 1108 8 0.05012103680208213 1108 9 0.10477706219251104 1108 10-11 0.08542722145555359 1108 12-13 0.10781183538678363 1108 14-15 0.22915022539130803 1108 16-17 0.17013319586527587 1108 18-19 0.2264604729424562 1108 20-21 0.24464042660991936 1108 22-23 0.17494126691087075 1108 24-25 0.1476412677558656 1108 26-27 0.1709341086067937 1108 28-29 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1.099664961234673 2316 82-83 1.3746725121376642 2316 84-85 1.6014577883509595 2316 86-87 1.7308627721026788 2316 88-89 1.9375590504505844 2316 90-91 2.0117578375426497 2316 92-93 1.9870913814157483 2316 94-95 2.6250440803491095 2316 96-97 2.993728618853627 2316 98-99 3.177275481468076 2316 100-101 3.526278696917153 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 19.0 4 65.0 5 99.0 6 255.0 7 674.0 8 1676.0 9 2875.0 10 4598.0 11 6962.0 12 10054.0 13 13679.0 14 17737.0 15 23478.0 16 29403.0 17 36359.0 18 35770.0 19 33425.0 20 34573.0 21 36421.0 22 39928.0 23 44843.0 24 51421.0 25 59067.0 26 69691.0 27 82477.0 28 97507.0 29 114872.0 30 135625.0 31 159571.0 32 189928.0 33 228095.0 34 283391.0 35 352768.0 36 431342.0 37 436963.0 38 194576.0 39 8602.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.96710470766607 18.35776842605045 14.736710397345076 23.93841646893841 2 16.548528258645987 19.747508228370727 38.0869963891271 25.61696712385618 3 19.904631435710808 22.65173189361697 29.33288505356107 28.110751617111152 4 14.075637000401064 16.10118340172241 34.3686683510784 35.454511246798134 5 14.593302387856847 36.63838658039826 33.41257024977391 15.355740781970978 6 34.99119094884142 34.53564109365005 15.869997433306505 14.603170524202023 7 30.419631442393026 31.751558753877955 19.106815137189614 18.721994666539402 8 27.99614047614459 34.14718013997199 18.53938926155402 19.317290122329407 9 26.777527022938408 16.19709024451503 18.792387535842696 38.23299519670386 10-11 26.11243524758497 25.621841919213708 26.29738096308826 21.968341870113072 12-13 26.992208448551562 22.561128757766234 26.694178316977162 23.75248447670504 14-15 23.12422582375106 24.21857337730058 24.62606273806541 28.03113806088295 16-17 23.395441809326183 27.398107010010104 25.73839856069374 23.468052619969974 18-19 23.647705868550418 25.64493910144925 27.669506549351336 23.037848480648996 20-21 23.615400405715008 24.97437130540685 28.129629952548292 23.28059833632985 22-23 23.582896092966312 24.80845399699033 28.22983501269733 23.378814897346025 24-25 23.644805246753016 25.197960880938346 27.937375115276737 23.2198587570319 26-27 22.905608226087683 25.625843103311652 27.74904839661599 23.71950027398467 28-29 23.237187870644764 26.153000629895338 27.497424893622945 23.11238660583695 30-31 23.253921855655154 25.486975789564177 27.399911955376854 23.85919039940382 32-33 22.829468083544217 25.491666014028457 27.486215057342424 24.192650845084902 34-35 23.768656128459508 25.604716415526646 27.252132932129395 23.374494523884447 36-37 24.056827090384136 24.997938775119984 27.289424271974603 23.65580986252127 38-39 23.29071635923946 25.44494030135699 27.302889228298966 23.961454111104587 40-41 23.758570857546967 25.237386538390055 27.512714691156955 23.49132791290603 42-43 23.579864128560065 25.33409900157697 27.10964266248792 23.976394207375044 44-45 23.33781931243065 25.544917968992593 27.604878754662014 23.51238396391474 46-47 24.036697921298533 25.898148109907766 26.700507878207734 23.364646090585968 48-49 23.599094712825543 26.081292291934922 26.456399709128466 23.86321328611107 50-51 23.845576828417308 26.107035707631894 26.647601548826678 23.399785915124127 52-53 24.308009949837814 25.53572461061363 26.711417074844352 23.444848364704203 54-55 23.46828565192113 25.93169993910603 26.94981165086294 23.650202758109902 56-57 23.602704603559047 26.133375673068716 26.608626965822594 23.655292757549642 58-59 23.496534046011423 25.626476651807987 26.67414036417679 24.2028489380038 60-61 22.93057660050471 25.968789129456493 27.12686043214063 23.973773837898165 62-63 23.288108744593195 25.9813931547294 27.19994542334688 23.53055267733053 64-65 22.855946698433705 26.143639387823715 26.93786123763574 24.062552676106836 66-67 22.77271975674183 26.17042909296267 26.932209329414047 24.124641820881454 68-69 23.592116224081764 25.567817317055336 27.10719474398623 23.732871714876673 70-71 23.236270101814217 25.896208911107056 26.500544389877987 24.366976597200743 72-73 23.20891594472643 26.043492167068877 26.531454782044335 24.216137106160357 74-75 23.197540257164945 26.523228576139786 26.333632831895038 23.945598334800234 76-77 23.33398188997359 26.00387849107791 26.658767736265055 24.003371882683446 78-79 23.341568778972814 26.322176017660322 26.637138984158394 23.699116219208474 80-81 23.702874652146335 26.29139693810763 26.28082729555887 23.72490111418717 82-83 23.427316955874105 26.382503951803145 26.430350827117678 23.759828265205073 84-85 23.153122600783718 26.710850105953675 26.32443622289416 23.811591070368447 86-87 23.707813212475774 26.584767915211664 25.900133430149097 23.80728544216347 88-89 23.223711964982837 26.854145424142278 25.569116099898526 24.35302651097636 90-91 23.057348535395764 26.817604313797833 25.98270234617518 24.14234480463123 92-93 23.274176850869495 26.358760494715472 26.209729722453705 24.157332931961324 94-95 22.95397970568377 25.97871632897363 26.487588553220036 24.579715412122564 96-97 23.26169229842049 25.566515754878626 26.48931701785091 24.68247492884997 98-99 22.925024099079874 26.135471245231805 25.952406955831155 24.987097699857163 100-101 22.97329088722003 25.605946208545593 26.49095746047751 24.929805443756862 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 448.0 1 310.0 2 241.0 3 338.0 4 311.5 5 303.0 6 579.5 7 1329.5 8 2035.0 9 2259.0 10 2021.0 11 1697.0 12 1773.0 13 1956.5 14 2092.5 15 2263.5 16 2505.5 17 2795.5 18 3153.5 19 3608.0 20 4176.5 21 4955.0 22 6010.5 23 7350.5 24 8966.0 25 11290.0 26 14364.5 27 18031.5 28 21366.0 29 24593.0 30 28516.0 31 35775.0 32 48749.0 33 51345.5 34 45928.5 35 47121.5 36 51650.5 37 57392.0 38 64016.0 39 69609.0 40 74338.5 41 82251.5 42 87038.5 43 90000.5 44 95888.0 45 105040.0 46 111410.0 47 115877.5 48 119436.0 49 120205.5 50 122448.0 51 123592.5 52 125785.5 53 132791.5 54 140654.5 55 135701.5 56 124763.5 57 116353.0 58 110256.5 59 99179.0 60 80312.0 61 67386.0 62 58860.0 63 45177.0 64 31633.5 65 23879.5 66 18240.0 67 14527.0 68 11213.5 69 8441.0 70 6426.0 71 4446.0 72 3206.5 73 2486.0 74 1878.0 75 1446.5 76 1104.5 77 786.0 78 516.0 79 314.0 80 183.5 81 115.5 82 81.0 83 52.5 84 29.0 85 16.5 86 10.5 87 7.0 88 4.0 89 1.0 90 1.0 91 1.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.05922943517141E-5 2 0.04414468074952345 3 1.529614717585705E-4 4 0.0 5 0.10658355352137193 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 9.942495664307083E-4 26-27 0.011196779732727362 28-29 0.0 30-31 0.0 32-33 3.9769982657228333E-4 34-35 0.014301897609426344 36-37 0.003349856231512694 38-39 0.011655664148003073 40-41 0.0072044853198286705 42-43 0.005889016662704964 44-45 0.002875675669061126 46-47 4.588844152757115E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.529614717585705E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.9884991328614167E-4 68-69 2.447383548137128E-4 70-71 0.0 72-73 4.588844152757115E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.588844152757115E-5 82-83 0.0011013225966617077 84-85 0.0046347325942846865 86-87 0.0013919493930029917 88-89 0.0 90-91 9.177688305514231E-4 92-93 2.7533064916542693E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.648073587928525E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3268797.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.05163883240519 #Duplication Level Percentage of deduplicated Percentage of total 1 69.40733380608947 29.18692133533861 2 15.120659701617495 12.71697041360245 3 5.7657691714054815 7.2738012836087815 4 2.964587788921191 4.986630999466904 5 1.637826047461805 3.443663470908477 6 1.10065603755497 2.7770634101980685 7 0.7313844360586168 2.1529139906945503 8 0.5384066043940536 1.811270405836832 9 0.38047888823212866 1.4399784712163282 >10 2.1150810011278867 16.122423685568872 >50 0.15042527707443454 4.356387588359257 >100 0.07476614063865458 5.734932973408843 >500 0.007370722785418626 2.0649486175529113 >1k 0.004670557011795779 4.359598524570701 >5k 5.838196264744724E-4 1.5724948296684333 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8226 0.2516522133372002 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6686 0.20454008003556048 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6675 0.20420356479769164 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6623 0.2026127654914025 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6270 0.19181368558524742 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5836 0.1785366298366035 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5623 0.1720204711396884 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5302 0.16220034465278815 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4807 0.1470571589486897 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4717 0.14430385245703542 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4444 0.13595215609901748 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4305 0.1316998271841292 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4279 0.13090442753098464 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 4168 0.12750868285794437 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3620 0.11074410555320506 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3358 0.10272892443305595 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3344 0.10230063231213196 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.177688305514231E-5 2 0.0 0.0 0.0 0.0 9.177688305514231E-5 3 0.0 0.0 0.0 0.0 9.177688305514231E-5 4 0.0 0.0 0.0 0.0 9.177688305514231E-5 5 0.0 0.0 0.0 0.0 9.177688305514231E-5 6 0.0 0.0 0.0 0.0 9.177688305514231E-5 7 3.05922943517141E-5 0.0 0.0 0.0 9.177688305514231E-5 8 3.05922943517141E-5 0.0 0.0 0.0 9.177688305514231E-5 9 3.05922943517141E-5 0.0 0.0 0.0 9.177688305514231E-5 10-11 3.05922943517141E-5 0.0 0.0 0.0 1.3766532458271344E-4 12-13 3.05922943517141E-5 0.0 0.0 0.0 6.424381813859962E-4 14-15 6.11845887034282E-5 0.0 0.0 0.0 9.483611249031372E-4 16-17 6.11845887034282E-5 0.0 0.0 0.0 0.0012083956268927071 18-19 6.11845887034282E-5 0.0 0.0 1.529614717585705E-5 0.0012848763627719923 20-21 6.11845887034282E-5 0.0 0.0 6.11845887034282E-5 0.0015449108647615623 22-23 6.11845887034282E-5 0.0 0.0 1.6825761893442755E-4 0.0016060954534649905 24-25 6.11845887034282E-5 0.0 0.0 4.129959737481404E-4 0.0016519838949925615 26-27 6.11845887034282E-5 0.0 0.0 9.330649777272802E-4 0.0017590569252235607 28-29 6.11845887034282E-5 0.0 0.0 0.0028144910803576976 0.001789649219575275 30-31 6.11845887034282E-5 0.0 0.0 0.011074410555320505 0.0018508338082787032 32-33 7.648073587928525E-5 0.0 0.0 0.027227141973025552 0.0019273145441579885 34-35 1.0707303023099935E-4 0.0 0.0 0.05226693489990354 0.002049683721564845 36-37 1.529614717585705E-4 0.0 0.0 0.09281702106310058 0.002049683721564845 38-39 1.529614717585705E-4 0.0 0.0 0.15865163850798933 0.0020802760159165587 40-41 1.8355376611028462E-4 0.0 0.0 0.2290751001056352 0.002110868310268273 42-43 2.141460604619987E-4 0.0 0.0 0.30425566347497257 0.00212616445744413 44-45 2.447383548137128E-4 0.0 0.0 0.3949924085221566 0.0021873490461475583 46-47 2.7533064916542693E-4 0.0 0.0 0.5046810799202276 0.0022485336348509865 48-49 2.7533064916542693E-4 0.0 0.0 0.6216201250796547 0.002325014370730272 50-51 2.7533064916542693E-4 0.0 0.0 0.7515455991913844 0.0023861989594337 52-53 2.7533064916542693E-4 0.0 0.0 0.8765610100596641 0.0028144910803576976 54-55 2.7533064916542693E-4 0.0 0.0 1.015893614684546 0.0028756756690611254 56-57 2.7533064916542693E-4 0.0 0.0 1.1772679673898379 0.002921564110588697 58-59 2.7533064916542693E-4 0.0 0.0 1.3458926938564861 0.002967452552116268 60-61 2.7533064916542693E-4 0.0 0.0 1.534968369097255 0.003028637140819696 62-63 2.7533064916542693E-4 0.0 0.0 1.7207859649895665 0.0031815986125782666 64-65 2.7533064916542693E-4 0.0 0.0 1.9145422612661478 0.003349856231512694 66-67 2.7533064916542693E-4 0.0 0.0 2.153345710975628 0.003411040820216122 68-69 2.90626796341284E-4 0.0 0.0 2.4180455378538346 0.0034569292617436936 70-71 3.05922943517141E-4 0.0 0.0 2.689995738493397 0.003487521556095408 72-73 3.05922943517141E-4 0.0 0.0 2.9734945302507314 0.0037322599109091206 74-75 3.05922943517141E-4 0.0 0.0 3.258400567548245 0.003915813677019405 76-77 3.05922943517141E-4 0.0 0.0 3.5737765300200657 0.004068775148777975 78-79 3.05922943517141E-4 0.0 0.0 3.9301155746288314 0.004191144326184832 80-81 3.05922943517141E-4 0.0 0.0 4.290814021182716 0.004267625062064117 82-83 3.05922943517141E-4 0.0 0.0 4.6771335142561625 0.004497067269701973 84-85 3.365152378688551E-4 0.0 0.0 5.079575146452961 0.004527659564053687 86-87 3.518113850447122E-4 0.0 0.0 5.4891753755280615 0.004619436447108829 88-89 3.6710753222056925E-4 0.0 0.0 5.918951222728117 0.0047723979188674 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4460 0.0 54.495014 1 GTATCAA 8195 0.0 42.77493 1 AGGTAAG 2105 0.0 32.085365 2 TATCAAC 11095 0.0 31.294378 2 ATCAACG 11060 0.0 31.16502 3 TCAACGC 11125 0.0 31.068401 4 TAAGGTG 2325 0.0 30.877241 5 CAACGCA 11560 0.0 29.940434 5 AAGGTAA 2280 0.0 28.371008 1 AACGCAG 12210 0.0 28.24057 6 GGTAAGG 2640 0.0 27.373112 3 TGGTATC 2025 0.0 27.011179 2 GTAAGGT 2615 0.0 26.907574 4 GTGGTAT 2215 0.0 26.626781 1 TACATGG 10670 0.0 25.007437 2 GTACATG 11065 0.0 24.845467 1 ACGCAGA 13835 0.0 24.786234 7 CGCAGAG 13980 0.0 24.52915 8 ACATGGG 10755 0.0 23.38459 3 GTGTAAG 1135 0.0 21.791054 1 >>END_MODULE