FastQCFastQC Report
Thu 26 May 2016
SRR938380_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938380_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3296369
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75960.23043536691432298No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA67480.20471009161899048No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA65610.19903718303381693No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA61700.18717564690118127No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA58790.1783477517231839No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58380.1771039589317822No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA50280.1525314671992122No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC50020.15174272055100627No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG49820.15113599236007863No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA47180.1431271802398336No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA44790.1358767783582481No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG40700.1234691868537776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40520.12292313148194271No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT37460.1136401901607496No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA36540.11084924048248238No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG33550.10177865402811397No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT33520.10168764479947481No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43650.053.7757841
GTATCAA82000.046.1386831
TATCAAC108500.034.7358472
ATCAACG109950.034.277763
TCAACGC110250.034.0120544
CAACGCA115300.032.5223665
AACGCAG120850.031.1017746
TAAGGTG21850.027.1889865
ACGCAGA137850.027.1566317
CGCAGAG137850.027.0187828
AAGGTAA20100.024.6855721
AGGTAAG21000.024.6684382
GTACATG115350.024.6510011
GTGTAAG13550.024.6470341
GTAAGGT23800.024.5619354
TACATGG113050.024.5093862
TGGTATC20850.023.9341322
GTGGTAT22700.023.749721
GCAGAGT154350.023.5753889
ACATGGG114350.023.3579443