##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938380_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3296369 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.03745029758501 31.0 31.0 34.0 28.0 34.0 2 31.20627180998244 31.0 31.0 34.0 28.0 34.0 3 31.261127319180588 31.0 31.0 34.0 27.0 34.0 4 34.939501615262124 37.0 35.0 37.0 32.0 37.0 5 34.91484236139825 37.0 35.0 37.0 32.0 37.0 6 34.89434283601138 37.0 35.0 37.0 32.0 37.0 7 34.795224685100486 37.0 35.0 37.0 32.0 37.0 8 34.74094041049409 37.0 35.0 37.0 31.0 37.0 9 36.220331522350804 39.0 35.0 39.0 32.0 39.0 10-11 36.04301187154715 38.0 35.0 39.0 30.0 39.0 12-13 35.846668409999 38.0 35.0 39.0 30.0 39.0 14-15 36.99330975992069 39.0 36.0 41.0 31.0 41.0 16-17 36.90896589550502 39.0 36.0 41.0 31.0 41.0 18-19 36.88321619940001 39.0 36.0 41.0 30.5 41.0 20-21 36.79132569806353 39.0 36.0 41.0 30.0 41.0 22-23 36.6307943679849 39.0 36.0 41.0 30.0 41.0 24-25 36.45862629456836 39.0 36.0 41.0 29.5 41.0 26-27 36.21975740580014 39.0 35.0 40.5 29.0 41.0 28-29 35.97136819330603 39.0 35.0 40.0 27.5 41.0 30-31 35.67257685653517 38.0 34.5 40.0 27.0 41.0 32-33 35.351550296705256 38.0 34.0 40.0 26.5 41.0 34-35 35.026888979965534 38.0 34.0 40.0 25.5 41.0 36-37 34.76705201996499 38.0 33.5 40.0 24.5 41.0 38-39 34.47690701496101 38.0 33.0 40.0 24.0 41.0 40-41 34.15870007271637 38.0 33.0 40.0 23.5 41.0 42-43 33.69263559389134 38.0 32.5 40.0 21.5 41.0 44-45 33.18615695026861 37.0 31.5 40.0 18.5 41.0 46-47 32.66979500777977 37.0 31.0 40.0 16.0 41.0 48-49 32.12745736293479 36.5 30.5 39.0 11.5 40.5 50-51 31.36242817475835 35.5 29.5 38.5 9.0 40.0 52-53 31.318676246500317 35.5 30.0 39.0 8.0 40.0 54-55 31.220336072812238 36.0 29.5 39.0 6.5 40.0 56-57 30.721256934524018 35.0 28.5 39.0 2.0 40.0 58-59 30.1998674905631 35.0 28.0 38.5 2.0 40.0 60-61 29.58271009707954 34.5 27.5 38.0 2.0 40.0 62-63 28.79453938560883 34.0 26.0 38.0 2.0 40.0 64-65 28.072947233759326 33.5 25.5 37.0 2.0 39.5 66-67 27.31535289283451 33.0 23.5 36.5 2.0 39.0 68-69 26.400055181928963 32.0 21.0 36.0 2.0 39.0 70-71 25.490541259185484 31.5 17.5 35.0 2.0 38.0 72-73 24.54518577865524 31.0 10.5 35.0 2.0 37.5 74-75 23.569237849282043 30.0 4.5 34.0 2.0 36.5 76-77 22.58380964024355 29.0 2.0 34.0 2.0 36.0 78-79 21.565760993383932 28.5 2.0 34.0 2.0 35.5 80-81 20.57862165916498 26.5 2.0 33.5 2.0 35.0 82-83 19.54741216775185 26.0 2.0 33.0 2.0 35.0 84-85 18.555346959032804 24.5 2.0 32.0 2.0 34.5 86-87 17.552457719387604 21.5 2.0 32.0 2.0 34.0 88-89 16.419520994160543 16.0 2.0 31.0 2.0 34.0 90-91 15.254781549031676 4.5 2.0 31.0 2.0 34.0 92-93 14.110272393654958 2.0 2.0 30.0 2.0 34.0 94-95 12.876822649406058 2.0 2.0 29.0 2.0 34.0 96-97 11.527150176451727 2.0 2.0 28.0 2.0 33.5 98-99 9.927390410478925 2.0 2.0 22.5 2.0 32.5 100-101 7.001585684126989 2.0 2.0 2.0 2.0 27.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5791906018229547 1101 2 0.49534890126021835 1101 3 0.5083309538482759 1101 4 0.3844208004858629 1101 5 0.3823144755351251 1101 6 0.6181349341230558 1101 7 0.4680010150706906 1101 8 0.4461828590383945 1101 9 0.5296355977070846 1101 10-11 0.6177018781705996 1101 12-13 0.5208793358760175 1101 14-15 0.6045493358221421 1101 16-17 0.6238554658155451 1101 18-19 0.6591395476664559 1101 20-21 0.7797320350373909 1101 22-23 0.8850459587145494 1101 24-25 0.8908334328252678 1101 26-27 0.8915027102520767 1101 28-29 0.9003330509295111 1101 30-31 1.0094177097301014 1101 32-33 0.9896233238833148 1101 34-35 1.1697132265358832 1101 36-37 1.1467354217902113 1101 38-39 1.177602082839904 1101 40-41 1.2059559724848796 1101 42-43 1.3131908581570357 1101 44-45 1.4256415206026176 1101 46-47 1.4075168587479396 1101 48-49 1.2073688479323437 1101 50-51 1.396418065229998 1101 52-53 1.4642662318946797 1101 54-55 1.6844877546696324 1101 56-57 1.9054512930945329 1101 58-59 1.7997359359405323 1101 60-61 1.775706670445043 1101 62-63 1.7928625477665499 1101 64-65 1.9757369582424325 1101 66-67 1.9913181829147248 1101 68-69 2.227204540845726 1101 70-71 2.635091439508063 1101 72-73 2.842072075025449 1101 74-75 2.868198243481043 1101 76-77 3.1801497524370106 1101 78-79 3.1969008689420235 1101 80-81 3.560106996592623 1101 82-83 3.672289616459029 1101 84-85 3.770226135970777 1101 86-87 3.7218247089853342 1101 88-89 3.8893130410399444 1101 90-91 4.0191551058379815 1101 92-93 4.093367116865338 1101 94-95 4.085042629322489 1101 96-97 3.916832657332529 1101 98-99 3.4688916357537547 1101 100-101 2.6390577331022307 1102 1 0.7747586526399886 1102 2 0.6853752462160827 1102 3 0.6025050254188322 1102 4 0.4894601671774623 1102 5 0.5452238623802188 1102 6 0.6159852033725173 1102 7 0.5799823355392206 1102 8 0.6350531768158092 1102 9 0.7107035362947158 1102 10-11 0.8255745822704768 1102 12-13 0.8378071695213478 1102 14-15 0.9066187687796372 1102 16-17 0.9211552411177593 1102 18-19 0.8981660214885139 1102 20-21 0.9253472938975804 1102 22-23 0.8982257899237354 1102 24-25 1.087813726592394 1102 26-27 0.8928677083926146 1102 28-29 0.9880175791367805 1102 30-31 0.9778596016475873 1102 32-33 0.9927618866594443 1102 34-35 1.0899558994082241 1102 36-37 1.2868725346837948 1102 38-39 1.3595480947069944 1102 40-41 1.4249512445111137 1102 42-43 1.532968289146062 1102 44-45 1.6143335673525527 1102 46-47 1.5852111594613376 1102 48-49 1.6790161255375153 1102 50-51 1.7094688372230529 1102 52-53 1.7090121770675388 1102 54-55 1.7929786120636635 1102 56-57 2.060274464228659 1102 58-59 2.148365266584534 1102 60-61 2.2812332561288393 1102 62-63 2.4111442944862063 1102 64-65 2.5731077753993183 1102 66-67 2.8255646401514625 1102 68-69 2.8820866760758115 1102 70-71 2.7596536667129463 1102 72-73 2.7758834755840383 1102 74-75 2.8434867723126054 1102 76-77 2.883929943846507 1102 78-79 3.3792859634068293 1102 80-81 3.5915656975515446 1102 82-83 3.7215807053342367 1102 84-85 3.5900269871494395 1102 86-87 3.8327216507755004 1102 88-89 4.0030802888422805 1102 90-91 3.9943012108711002 1102 92-93 3.8197662204272476 1102 94-95 3.712113702980089 1102 96-97 3.3421583294083277 1102 98-99 2.9943651362871417 1102 100-101 2.0658288297939738 1103 1 0.5097739280221774 1103 2 0.47834619368292053 1103 3 0.41212780139252203 1103 4 0.3103097593454862 1103 5 0.3303227520736627 1103 6 0.36064248298205825 1103 7 0.3701030875426383 1103 8 0.4126312460275159 1103 9 0.5817209110590937 1103 10-11 0.5619401689144894 1103 12-13 0.5963841590201469 1103 14-15 0.642399644958985 1103 16-17 0.68012729438788 1103 18-19 0.8594812233172675 1103 20-21 0.7605852846380046 1103 22-23 0.7770146583374427 1103 24-25 0.8569894420942958 1103 26-27 0.8947861555788919 1103 28-29 1.050490684599815 1103 30-31 0.919746895846302 1103 32-33 0.9907495766456762 1103 34-35 1.0351262481544268 1103 36-37 1.1079914742992116 1103 38-39 1.0936699979038167 1103 40-41 1.23233752976261 1103 42-43 1.3312002011850197 1103 44-45 1.5351883061163463 1103 46-47 1.7196196458403747 1103 48-49 1.5501216893649499 1103 50-51 1.7399312338354527 1103 52-53 1.831903010980458 1103 54-55 1.8973975006420076 1103 56-57 2.048237997380955 1103 58-59 2.014970284300972 1103 60-61 2.2855307976286277 1103 62-63 2.5682135279936915 1103 64-65 2.6330242915399715 1103 66-67 2.8544501323268463 1103 68-69 2.8586596133452424 1103 70-71 3.0258914444414806 1103 72-73 3.2662334298007814 1103 74-75 3.282375951094618 1103 76-77 3.4321656532868623 1103 78-79 3.6669319712568154 1103 80-81 3.8565798185237092 1103 82-83 3.8756390462861994 1103 84-85 3.929105222910703 1103 86-87 4.025874775223759 1103 88-89 4.254015432443023 1103 90-91 4.247014476507637 1103 92-93 4.169358299101539 1103 94-95 4.160729009599253 1103 96-97 4.041839186859777 1103 98-99 3.6520497721976586 1103 100-101 2.442466001224936 1104 1 0.4848346047436465 1104 2 0.4192877769794947 1104 3 0.37327916806836825 1104 4 0.3965177300163347 1104 5 0.3346722858845368 1104 6 0.5461519810633888 1104 7 0.4715122509010783 1104 8 0.3418579595236295 1104 9 0.5467344649547243 1104 10-11 0.5020146152480365 1104 12-13 0.6324644169272773 1104 14-15 0.6371556213399288 1104 16-17 0.6722237890129179 1104 18-19 0.6950159861085652 1104 20-21 0.669986276312379 1104 22-23 0.8128584047349747 1104 24-25 0.7739443059306268 1104 26-27 0.7452293406994954 1104 28-29 0.7998475475926057 1104 30-31 0.850533023518679 1104 32-33 0.8737025673729661 1104 34-35 0.8026710705800966 1104 36-37 0.9612460918391292 1104 38-39 1.010877897129376 1104 40-41 1.0830589459531481 1104 42-43 1.0145532251153995 1104 44-45 1.1498611833457417 1104 46-47 1.2864489023054801 1104 48-49 1.2334464112978267 1104 50-51 1.2861938687790264 1104 52-53 1.4116003573575604 1104 54-55 1.7631847690229492 1104 56-57 1.7936169628532106 1104 58-59 2.0234767171379175 1104 60-61 2.0684426986876048 1104 62-63 2.1140606499221484 1104 64-65 2.1944533888236 1104 66-67 2.469223058843127 1104 68-69 2.723877002023478 1104 70-71 2.7545019509713065 1104 72-73 3.0281637571858653 1104 74-75 2.947895006277303 1104 76-77 3.0930101762636006 1104 78-79 3.355729678242785 1104 80-81 3.551209031293297 1104 82-83 3.561979052678307 1104 84-85 3.8928803486992756 1104 86-87 3.8729243523850165 1104 88-89 3.9394563326950482 1104 90-91 3.6444630945763237 1104 92-93 3.455623253428328 1104 94-95 3.566816726118164 1104 96-97 3.4057991643838488 1104 98-99 3.214037445642308 1104 100-101 2.2746838216812044 1105 1 0.36750729411822647 1105 2 0.3562054232130478 1105 3 0.2531569602097896 1105 4 0.2838251987754177 1105 5 0.25110796516782585 1105 6 0.27195517580429396 1105 7 0.29410041192083014 1105 8 0.24203032321101148 1105 9 0.2963426382002723 1105 10-11 0.39266238203595805 1105 12-13 0.43204603413959575 1105 14-15 0.46016454516920646 1105 16-17 0.49836281736273946 1105 18-19 0.5538774337631125 1105 20-21 0.47280134109907834 1105 22-23 0.5116387061710057 1105 24-25 0.5425018373218577 1105 26-27 0.3841539716708482 1105 28-29 0.5563111858693759 1105 30-31 0.5899406736604789 1105 32-33 0.6165290562645325 1105 34-35 0.5928224149166468 1105 36-37 0.7276419370725762 1105 38-39 0.7912278104464718 1105 40-41 1.1658292162234218 1105 42-43 1.1599566513447428 1105 44-45 1.0059803447763542 1105 46-47 1.1171139806207222 1105 48-49 1.0982671831263886 1105 50-51 0.9702050072812689 1105 52-53 1.0442562613149171 1105 54-55 1.3335354589787585 1105 56-57 1.4801014451107655 1105 58-59 1.4246451826000381 1105 60-61 1.626730936870171 1105 62-63 1.6001892562815634 1105 64-65 1.5645184761578754 1105 66-67 1.8602801893885825 1105 68-69 2.0355977327653427 1105 70-71 2.0729496255646964 1105 72-73 2.12326717539829 1105 74-75 2.2519131094750477 1105 76-77 2.43553202235886 1105 78-79 2.6381161386742917 1105 80-81 2.8881340681210013 1105 82-83 3.0457655922768616 1105 84-85 3.0830916946392115 1105 86-87 3.057167605649404 1105 88-89 3.2530035423073294 1105 90-91 3.193111617960664 1105 92-93 3.3941781041633803 1105 94-95 3.2625671344798075 1105 96-97 3.120398719445028 1105 98-99 2.9413469436831736 1105 100-101 1.9943007133225663 1106 1 0.24438145204072725 1106 2 0.10083637067413065 1106 3 0.057825639462677714 1106 4 0.18335019146761056 1106 5 0.1435081842339727 1106 6 -0.18438791470832427 1106 7 0.11820323189348159 1106 8 -0.1615785563597285 1106 9 0.12158715566676648 1106 10-11 0.18019325121112928 1106 12-13 0.3462092316989569 1106 14-15 0.4181506737798486 1106 16-17 -0.021926042518927602 1106 18-19 0.3675157293478577 1106 20-21 0.3810586984624891 1106 22-23 0.29381232449191685 1106 24-25 0.4097450225839552 1106 26-27 0.3525105051842701 1106 28-29 0.29718398974804927 1106 30-31 0.21254463768256215 1106 32-33 0.34135917080387657 1106 34-35 0.3466787938741973 1106 36-37 0.50190258882796 1106 38-39 0.5932173392862623 1106 40-41 0.715065550628573 1106 42-43 0.38304213250068386 1106 44-45 0.718997560956339 1106 46-47 0.7902805196108034 1106 48-49 0.7692956852448063 1106 50-51 0.6239255618680524 1106 52-53 0.8826738107979857 1106 54-55 0.7366787389903138 1106 56-57 1.1975975056160344 1106 58-59 1.3352477153003655 1106 60-61 1.4514139293406139 1106 62-63 1.4872942148370711 1106 64-65 1.5729862059001398 1106 66-67 1.6753587944882504 1106 68-69 1.8723433909213547 1106 70-71 1.7038610516113941 1106 72-73 1.9005829935196346 1106 74-75 1.942051310982304 1106 76-77 2.1156904505256584 1106 78-79 2.453023934337992 1106 80-81 2.0623320155518456 1106 82-83 2.2665260575572255 1106 84-85 2.2087220977948405 1106 86-87 2.198595130705936 1106 88-89 1.9910802708971467 1106 90-91 1.8673665998675482 1106 92-93 1.9325874218664119 1106 94-95 1.7739556761779731 1106 96-97 1.8142520156849518 1106 98-99 1.715294829795658 1106 100-101 1.0611509990829333 1107 1 0.27229710246153616 1107 2 0.21845233727001911 1107 3 0.27051900986804966 1107 4 0.17444669933532708 1107 5 0.22326947679722764 1107 6 0.1377559120543097 1107 7 0.21565825002305417 1107 8 0.2868688501961216 1107 9 0.26230265930954033 1107 10-11 0.20305837384967873 1107 12-13 0.30430858707365616 1107 14-15 0.41116578726932573 1107 16-17 0.37522548485317486 1107 18-19 0.38899089606891835 1107 20-21 0.4563900128886047 1107 22-23 0.4317206654713104 1107 24-25 0.40903351409266264 1107 26-27 0.444338227247421 1107 28-29 0.5121120508344248 1107 30-31 0.5400925574050603 1107 32-33 0.6752734732497672 1107 34-35 0.6084086797349713 1107 36-37 0.7507092807803417 1107 38-39 0.8523042221286516 1107 40-41 0.8608880068169285 1107 42-43 1.018512179012042 1107 44-45 0.7938486306781982 1107 46-47 0.7789231026554546 1107 48-49 0.6309073859812813 1107 50-51 0.982977239586166 1107 52-53 1.1424928652973598 1107 54-55 1.1984969731824577 1107 56-57 1.4491001064743791 1107 58-59 1.4792020167249689 1107 60-61 1.5644331803732783 1107 62-63 1.7210595951882617 1107 64-65 1.7227308173556999 1107 66-67 1.9251310086597968 1107 68-69 2.081808683840759 1107 70-71 2.239276162744037 1107 72-73 2.2798847372780457 1107 74-75 2.37215113575866 1107 76-77 2.5838460162577697 1107 78-79 2.969846165318309 1107 80-81 2.983756952367571 1107 82-83 2.8643314493259417 1107 84-85 2.879403365231685 1107 86-87 2.8040037900208254 1107 88-89 3.1092376942683053 1107 90-91 3.0777647631126115 1107 92-93 3.0784330371672013 1107 94-95 2.929848254558067 1107 96-97 2.7997653771016413 1107 98-99 2.3613359063686854 1107 100-101 1.4251450196128719 1108 1 0.29749291422495716 1108 2 0.15801080029475045 1108 3 0.11994931004215559 1108 4 0.19920065408502552 1108 5 0.22871075805549168 1108 6 0.14702973224980553 1108 7 0.15766775265505828 1108 8 0.22413323364988003 1108 9 0.2646793370405476 1108 10-11 0.24459956138688455 1108 12-13 0.254847585171305 1108 14-15 0.32983758375499406 1108 16-17 0.43431234925131434 1108 18-19 0.3295415175239853 1108 20-21 0.3859840237140659 1108 22-23 0.3601832854504252 1108 24-25 0.48134828163963306 1108 26-27 0.49211734078483005 1108 28-29 0.5286278784617124 1108 30-31 0.44836796743864227 1108 32-33 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-0.7956824997821172 2315 86-87 -0.6722618085428529 2315 88-89 -0.6119630878266875 2315 90-91 -0.7475870181538866 2315 92-93 -0.5599541440150624 2315 94-95 -0.5154042719594099 2315 96-97 -0.4391222631478424 2315 98-99 -0.5995225163778475 2315 100-101 -0.2977605397471894 2316 1 -0.9964497653496274 2316 2 -0.9152686557908751 2316 3 -0.7433646071127029 2316 4 -0.7843593609350776 2316 5 -0.6633908770877639 2316 6 -0.4845645890418737 2316 7 -0.7789969356659299 2316 8 -0.7305491590623276 2316 9 -0.8593816106523775 2316 10-11 -0.8847293653955148 2316 12-13 -0.897313532156879 2316 14-15 -1.1305161322845834 2316 16-17 -1.133013269322504 2316 18-19 -1.040467990026798 2316 20-21 -0.9148321114749933 2316 22-23 -1.092518254853914 2316 24-25 -0.8920421015048632 2316 26-27 -1.1094357985827443 2316 28-29 -1.1330008227825203 2316 30-31 -0.9675427227595179 2316 32-33 -1.0773430441749952 2316 34-35 -0.9613699023746491 2316 36-37 -1.1964252814291498 2316 38-39 -1.3963838044336612 2316 40-41 -1.1739627017500354 2316 42-43 -0.9250944072634653 2316 44-45 -1.0474481731721497 2316 46-47 -0.9352640749232748 2316 48-49 -0.6986623677797041 2316 50-51 -0.5122452859677615 2316 52-53 -0.6966960007928762 2316 54-55 -0.798103546608921 2316 56-57 -0.723539557066065 2316 58-59 -0.45950619768086653 2316 60-61 -0.6744618171750041 2316 62-63 -0.5204537771474378 2316 64-65 -0.41094323508541564 2316 66-67 -0.3216576113130003 2316 68-69 -0.4046221000203154 2316 70-71 -0.415247967436148 2316 72-73 -0.2785193559439989 2316 74-75 -0.026455268157608458 2316 76-77 0.016956983882923993 2316 78-79 -0.03509577014813203 2316 80-81 -0.1177224201005096 2316 82-83 0.017036546064140623 2316 84-85 0.056357851066426434 2316 86-87 0.2754419425460952 2316 88-89 0.24491752952361523 2316 90-91 0.12330222983205097 2316 92-93 0.04035338805340061 2316 94-95 0.02113357653019321 2316 96-97 -0.0315930168942824 2316 98-99 -0.3345121789268113 2316 100-101 0.05330304267658725 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11302.0 3 4419.0 4 5000.0 5 5812.0 6 7259.0 7 9346.0 8 12044.0 9 15613.0 10 19906.0 11 24453.0 12 29935.0 13 34968.0 14 39672.0 15 44909.0 16 51595.0 17 59092.0 18 60490.0 19 62594.0 20 67572.0 21 74358.0 22 82855.0 23 91280.0 24 101009.0 25 110996.0 26 121508.0 27 133445.0 28 145454.0 29 159869.0 30 175003.0 31 191877.0 32 210894.0 33 233619.0 34 251402.0 35 257372.0 36 228339.0 37 134051.0 38 26736.0 39 321.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.16563559839467 17.457186177889046 14.08065202290875 24.296526200807538 2 16.05528919099351 18.950901256296657 39.133610487346 25.860199065363844 3 19.820582050739695 22.36922005904933 28.770813158853866 29.03938473135711 4 13.479500331875832 15.313937540573983 34.65724525760515 36.549316869945045 5 14.698234357715897 36.49968490188245 33.295003478828924 15.507077261572721 6 35.09286020855226 34.04017335443503 16.15669890806618 14.710267528946536 7 30.546229568095928 31.59085481008014 19.1564094292435 18.706506192580434 8 28.067932343217294 33.632706969313844 18.780662935472105 19.518697751996754 9 27.126153192522455 16.26119810633649 18.797371934935665 37.81527676620539 10-11 26.149138028880266 25.366966685907226 26.303563267156726 22.180332018055783 12-13 27.106386058461375 22.46232198045178 26.593533177185886 23.837758783900963 14-15 23.3299782338299 24.066486804202484 24.605497649660247 27.998037312307368 16-17 23.560660758751993 27.252179435498856 25.660938535223327 23.52622127052582 18-19 23.827295677506445 25.547914933542877 27.5178764306948 23.106912958255883 20-21 23.78107032501352 24.913343283201616 27.975294869546058 23.330291522238806 22-23 23.65137301463866 24.747508696328385 28.04389701726368 23.557221271769272 24-25 23.653721136751145 25.08330971121121 27.873669093220077 23.389300058817568 26-27 22.981800944185274 25.483245506379742 27.595462496616204 23.939491052818777 28-29 23.26623298688415 26.105468458983182 27.38708613828501 23.241212415847656 30-31 23.23630316263529 25.417752211932832 27.281739016962575 24.0642056084693 32-33 22.830773516482367 25.47404544931086 27.373853529427738 24.32132750477903 34-35 23.864445190621293 25.565328853844537 27.10915628286388 23.461069672670288 36-37 24.088469415206568 24.90306853172022 27.133315459068918 23.875146594004292 38-39 23.322854556068386 25.407828832950834 27.117196441119606 24.15212016986117 40-41 23.819273158711766 25.195782152410544 27.368071660143755 23.61687302873393 42-43 23.605106005981632 25.280266753845094 26.97913861748873 24.135488622684544 44-45 23.382953405635597 25.58179999987864 27.39048925690645 23.644757337579318 46-47 24.050122849377335 25.94484787385997 26.544932377846603 23.46009689891609 48-49 23.65513031193792 26.035535095746564 26.317106946329005 23.992227645986507 50-51 23.867798288125968 26.14379917830168 26.491586641114317 23.496815892458038 52-53 24.310013552614453 25.49898469164389 26.626746070970555 23.5642556847711 54-55 23.45258348357482 25.98243520907016 26.85782288519781 23.707158422157214 56-57 23.605666697359837 26.118800163042927 26.570308317692202 23.705224821905034 58-59 23.550871214861946 25.51042774466167 26.650684774623617 24.288016265852765 60-61 22.963839978156088 25.857855276481008 27.09705751851613 24.081247226846777 62-63 23.308709809790294 25.872205960768728 27.257459304437926 23.561624925003052 64-65 22.94261426322238 26.01358602945627 26.94433568818241 24.099464019138946 66-67 22.87349527256312 26.032977306088323 26.90014809313591 24.193379328212643 68-69 23.75213885248289 25.410947890879083 27.093709043250332 23.7432042133877 70-71 23.396342624302456 25.75660831461714 26.44804938192945 24.398999679150958 72-73 23.353190675999162 25.869930613074597 26.54797770637832 24.22890100454792 74-75 23.36934986981134 26.355967531415487 26.373959112690404 23.900723486082768 76-77 23.616016406858538 25.796978777722412 26.4681946463826 24.118810169036454 78-79 23.692936647894268 26.084532681039878 26.34249198854694 23.880038682518915 80-81 24.102030429515978 26.016242525927435 25.968381155514564 23.91334588904202 82-83 23.782628384010852 26.08990350956371 26.119895112825013 24.007572993600427 84-85 23.617162901696133 26.3288033139912 26.06483872083435 23.98919506347832 86-87 24.16046372217928 26.26072268044865 25.560100403970047 24.018713193402025 88-89 23.77868194651144 26.3816562975504 25.286808087152156 24.552853668786 90-91 23.664549738901453 26.35770789188527 25.535116229742442 24.442626139470836 92-93 24.044633163376062 25.747199391266662 25.806012667684964 24.402154777672315 94-95 23.679040268570418 25.450090300248323 26.070692107709746 24.800177323471512 96-97 24.078934992035197 25.016581961617234 25.973526511416217 24.93095653493135 98-99 23.791655086960056 25.46855335989485 25.28833469458094 25.451456858564146 100-101 24.79481064806757 24.24171595736958 25.773319567475035 25.19015382708782 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 367.0 1 282.0 2 256.5 3 329.5 4 309.5 5 311.5 6 494.5 7 1015.0 8 1661.0 9 1934.5 10 1745.0 11 1515.0 12 1536.0 13 1683.0 14 1878.0 15 2088.0 16 2337.0 17 2657.5 18 3043.0 19 3484.5 20 4014.0 21 4728.0 22 5761.0 23 7250.0 24 9111.0 25 11350.0 26 14353.0 27 18098.5 28 21312.5 29 24541.0 30 28800.5 31 36120.0 32 46362.0 33 49214.0 34 46555.5 35 47746.5 36 51788.5 37 57563.5 38 63478.5 39 68288.5 40 73575.5 41 80648.0 42 85862.5 43 89671.5 44 94771.5 45 102310.0 46 109262.0 47 114679.0 48 118322.0 49 119751.5 50 120983.0 51 122348.0 52 125200.0 53 131114.5 54 136525.5 55 133550.0 56 126352.5 57 119352.5 58 113170.5 59 102146.0 60 85350.5 61 72439.0 62 62576.5 63 50213.5 64 37868.5 65 29000.0 66 22379.5 67 17432.5 68 13489.0 69 10410.0 70 8021.5 71 5892.0 72 4354.0 73 3270.5 74 2457.0 75 1898.0 76 1449.0 77 1058.5 78 716.5 79 456.5 80 280.5 81 197.0 82 143.5 83 82.5 84 50.5 85 35.0 86 25.5 87 18.5 88 11.0 89 5.0 90 3.5 91 3.0 92 1.5 93 1.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.380964631083474 2 0.029395980850444838 3 0.03512956225471117 4 0.04435183075681151 5 0.06543563539154748 6 0.05603134843216886 7 0.036889074008401364 8 0.03843623089526688 9 0.03546326275972138 10-11 0.03782950270433923 12-13 0.029714513150681857 14-15 0.026938731677187838 16-17 0.02225175640227171 18-19 0.027257263977424857 20-21 0.03588797249337074 22-23 0.035084057640391596 24-25 0.043532747699059175 26-27 0.038011521161617524 28-29 0.03277849051486651 30-31 0.035614944807453294 32-33 0.029835858788867388 34-35 0.03192907104756779 36-37 0.03423463817309288 38-39 0.015198541182737734 40-41 0.012817133033346691 42-43 0.013545206862459875 44-45 0.011861536132635637 46-47 0.02021921696266407 48-49 0.01774679958463388 50-51 0.011846367927862445 52-53 0.009889669512120761 54-55 0.008691381335038644 56-57 0.009237436706873534 58-59 0.004201592722174004 60-61 0.007781289048647164 62-63 0.012180068432872655 64-65 0.007963307505925459 66-67 0.0040195742648957075 68-69 0.006340309595193985 70-71 0.012938478671532222 72-73 0.010890771027151391 74-75 0.011618844856264575 76-77 0.005657740380400374 78-79 0.009298109525966297 80-81 0.011876704337408828 82-83 0.013545206862459875 84-85 0.008585203901626304 86-87 0.0025785948114425295 88-89 0.015896278602304536 90-91 0.026392676305352948 92-93 0.010450893088728841 94-95 0.021144477453828744 96-97 0.018778237509210892 98-99 0.011315480760800748 100-101 0.12638148217023032 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3296369.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.49632191573365 #Duplication Level Percentage of deduplicated Percentage of total 1 72.93539833766842 33.18292361822827 2 13.394232501106035 12.187766273690048 3 5.165162347551257 7.049876667336555 4 2.586932715686763 4.707836944289122 5 1.4660467228491276 3.334986682312513 6 0.948289622521346 2.588621396134842 7 0.6347401134216933 2.0214838373143857 8 0.4722940639935643 1.719011421947305 9 0.35102548480755336 1.4373331611687827 >10 1.849747891088021 15.238444452721586 >50 0.12243018400912864 3.8548886928298556 >100 0.0628825657665123 5.206110022431937 >500 0.006114210605354456 1.822529412603114 >1k 0.004165725905259744 4.155768119199486 >5k 5.375130200335154E-4 1.4924192977922404 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7596 0.23043536691432298 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6748 0.20471009161899048 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6561 0.19903718303381693 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6170 0.18717564690118127 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5879 0.1783477517231839 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5838 0.1771039589317822 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 5028 0.1525314671992122 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5002 0.15174272055100627 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4982 0.15113599236007863 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4718 0.1431271802398336 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4479 0.1358767783582481 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4070 0.1234691868537776 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4052 0.12292313148194271 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3746 0.1136401901607496 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3654 0.11084924048248238 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3355 0.10177865402811397 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3352 0.10168764479947481 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 6.067281909276541E-5 5 0.0 0.0 0.0 0.0 1.2134563818553081E-4 6 0.0 0.0 0.0 0.0 1.5168204773191352E-4 7 0.0 0.0 0.0 0.0 1.5168204773191352E-4 8 0.0 0.0 0.0 0.0 1.8201845727829622E-4 9 0.0 0.0 0.0 0.0 1.8201845727829622E-4 10-11 0.0 0.0 0.0 0.0 1.8201845727829622E-4 12-13 0.0 0.0 0.0 0.0 5.460553718348887E-4 14-15 0.0 0.0 0.0 0.0 6.674010100204195E-4 16-17 0.0 0.0 0.0 3.0336409546382703E-5 8.039148529791416E-4 18-19 0.0 0.0 0.0 3.0336409546382703E-5 9.252604911646724E-4 20-21 0.0 0.0 0.0 3.0336409546382703E-5 0.0011376153579893513 22-23 0.0 0.0 0.0 9.100922863914811E-5 0.0012741292009480734 24-25 3.0336409546382703E-5 0.0 0.0 3.3370050501020974E-4 0.0012741292009480734 26-27 3.0336409546382703E-5 0.0 0.0 8.342512625255244E-4 0.0013196338152676474 28-29 3.0336409546382703E-5 0.0 0.0 0.002730276859174443 0.0013651384295872216 30-31 3.0336409546382703E-5 0.0 0.0 0.01067841616032671 0.0014409794534531784 32-33 4.5504614319574055E-5 0.0 0.0 0.026089312209889123 0.0016078297059582832 34-35 7.584102386595676E-5 0.0 0.0 0.05064663573768592 0.0017898481632365793 36-37 1.2134563818553081E-4 0.0 0.0 0.09053901429117917 0.0018353527775561535 38-39 1.8201845727829622E-4 3.0336409546382703E-5 0.0 0.15429097895290242 0.0018960255966489188 40-41 1.8201845727829622E-4 3.0336409546382703E-5 0.0 0.22661297931147878 0.0019111938014221101 42-43 1.9718666205148757E-4 3.0336409546382703E-5 0.0 0.30083100526670403 0.001941530210968493 44-45 2.1235486682467892E-4 3.0336409546382703E-5 0.0 0.39275032619224365 0.0020022030300612583 46-47 2.1235486682467892E-4 3.0336409546382703E-5 0.0 0.5016883728733039 0.0021538850777931716 48-49 2.1235486682467892E-4 3.0336409546382703E-5 0.0 0.6181801855314135 0.00224489430643232 50-51 2.1235486682467892E-4 3.0336409546382703E-5 0.0 0.7493548204099723 0.0023055671255250852 52-53 2.1235486682467892E-4 3.0336409546382703E-5 0.0 0.8746745282460793 0.0023662399446178505 54-55 2.1235486682467892E-4 3.0336409546382703E-5 0.0 1.0122956501532443 0.002517921992349764 56-57 2.1235486682467892E-4 3.0336409546382703E-5 0.0 1.1722443694865472 0.0025330901971229555 58-59 2.1235486682467892E-4 3.0336409546382703E-5 0.0 1.3451012310818358 0.0025785948114425295 60-61 2.1235486682467892E-4 3.0336409546382703E-5 0.0 1.5332021384741816 0.002608931220988912 62-63 2.4269127637106163E-4 3.0336409546382703E-5 0.0 1.7211968684331154 0.002775781473494017 64-65 2.4269127637106163E-4 3.0336409546382703E-5 0.0 1.9125437716469242 0.002821286087813591 66-67 2.4269127637106163E-4 3.0336409546382703E-5 0.0 2.1433280072710303 0.002821286087813591 68-69 2.4269127637106163E-4 3.0336409546382703E-5 0.0 2.3986847346277074 0.002821286087813591 70-71 2.5785948114425295E-4 3.0336409546382703E-5 0.0 2.6620351058998555 0.0029122953164527395 72-73 2.7302768591744433E-4 3.0336409546382703E-5 0.0 2.939203711720381 0.003109481978504227 74-75 2.7302768591744433E-4 3.0336409546382703E-5 0.0 3.212944303262165 0.0032763322310093317 76-77 2.7302768591744433E-4 3.0336409546382703E-5 0.0 3.5109995270553753 0.0033218368453289057 78-79 2.7302768591744433E-4 3.0336409546382703E-5 0.0 3.845170246413554 0.0034280142787412454 80-81 3.0336409546382703E-4 6.067281909276541E-5 0.0 4.185635770752606 0.0034431824835144367 82-83 3.0336409546382703E-4 6.067281909276541E-5 0.0 4.546077820777953 0.003625200940792733 84-85 3.0336409546382703E-4 6.067281909276541E-5 0.0 4.915256756752657 0.0036403691455659244 86-87 3.0336409546382703E-4 6.067281909276541E-5 0.0 5.288728294678174 0.0037010419646586897 88-89 3.0336409546382703E-4 6.067281909276541E-5 0.0 5.667341854021804 0.0037768829885246463 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4365 0.0 53.775784 1 GTATCAA 8200 0.0 46.138683 1 TATCAAC 10850 0.0 34.735847 2 ATCAACG 10995 0.0 34.27776 3 TCAACGC 11025 0.0 34.012054 4 CAACGCA 11530 0.0 32.522366 5 AACGCAG 12085 0.0 31.101774 6 TAAGGTG 2185 0.0 27.188986 5 ACGCAGA 13785 0.0 27.156631 7 CGCAGAG 13785 0.0 27.018782 8 AAGGTAA 2010 0.0 24.685572 1 AGGTAAG 2100 0.0 24.668438 2 GTACATG 11535 0.0 24.651001 1 GTGTAAG 1355 0.0 24.647034 1 GTAAGGT 2380 0.0 24.561935 4 TACATGG 11305 0.0 24.509386 2 TGGTATC 2085 0.0 23.934132 2 GTGGTAT 2270 0.0 23.74972 1 GCAGAGT 15435 0.0 23.575388 9 ACATGGG 11435 0.0 23.357944 3 >>END_MODULE