FastQCFastQC Report
Thu 26 May 2016
SRR938380_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938380_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3296369
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78340.23765543238636208No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA66970.20316293473212496No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA65550.1988551645765386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65410.19843045484288926No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA63200.19172610833313866No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC58900.1786814522281941No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA57790.17531411076854564No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG53470.16220878184450832No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA47480.14403727252622509No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46870.1421867515438957No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA44750.1357554327200626No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA43180.13099261642128052No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG42340.12844435801938436No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA42240.12814099392392053No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT36820.11169865994978112No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT35190.10675382519372073No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT34340.1041752303822782No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG33760.102415718628588No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG33320.10108091660854716No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA32970.10001914227442377No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44900.052.4886971
GTATCAA79550.044.25971
TATCAAC110950.031.470112
TCAACGC111650.031.0132794
ATCAACG111800.030.971673
CAACGCA116050.029.8783515
AGGTAAG20250.029.3242
AACGCAG121550.028.5259556
TAAGGTG23900.028.419545
GTAAGGT24600.026.8385244
AAGGTAA22000.025.4853021
ACGCAGA139850.024.6913197
GTGGTAT22150.024.4546571
CGCAGAG141800.024.3517688
TACATGG104950.023.809022
TGGTATC21300.023.6409242
GTACATG108700.023.6044921
GTGTAAG11200.023.3332461
GGTAAGG27200.022.8760643
ACATGGG104300.022.5879213
Produced by FastQC (version 0.11.5)