##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938380_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3296369 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.783996269835082 31.0 31.0 34.0 30.0 34.0 2 32.048823720888045 31.0 31.0 34.0 30.0 34.0 3 32.102791890106964 33.0 31.0 34.0 30.0 34.0 4 35.764656808749265 37.0 35.0 37.0 35.0 37.0 5 35.64705225658899 37.0 35.0 37.0 33.0 37.0 6 35.683253907557074 37.0 35.0 37.0 33.0 37.0 7 35.598379307656394 37.0 35.0 37.0 33.0 37.0 8 35.56403970550627 37.0 35.0 37.0 33.0 37.0 9 37.11424479480301 39.0 37.0 39.0 33.0 39.0 10-11 37.036228347008475 39.0 37.0 39.0 33.0 39.0 12-13 36.91142981868838 39.0 37.0 39.0 33.0 39.0 14-15 37.92068303032822 40.0 37.0 41.0 33.0 41.0 16-17 38.00483789891241 40.0 37.0 41.0 33.0 41.0 18-19 38.13385561507222 40.0 37.0 41.0 33.0 41.0 20-21 38.07028930923692 40.0 37.0 41.0 33.0 41.0 22-23 37.94511309261797 40.0 37.0 41.0 33.0 41.0 24-25 37.799831875618295 40.0 37.0 41.0 32.0 41.0 26-27 37.56436688368323 39.5 37.0 41.0 32.0 41.0 28-29 37.4205406008854 39.0 36.5 41.0 31.5 41.0 30-31 37.14602976790523 39.0 36.0 41.0 31.0 41.0 32-33 37.033234598432394 39.0 36.0 41.0 31.0 41.0 34-35 36.89173602833906 39.0 35.5 41.0 30.0 41.0 36-37 36.740989252113465 39.0 35.0 40.5 30.0 41.0 38-39 36.500498427208846 39.0 35.0 40.0 30.0 41.0 40-41 36.25161048414179 38.5 35.0 40.0 30.0 41.0 42-43 35.964431014853005 38.0 35.0 40.0 29.0 41.0 44-45 35.66004761602842 38.0 34.5 40.0 28.0 41.0 46-47 35.22835535099377 38.0 34.0 40.0 27.0 41.0 48-49 34.77682474261832 38.0 33.5 40.0 26.0 41.0 50-51 34.32781842688121 38.0 33.0 40.0 24.0 41.0 52-53 33.670156162735424 37.0 32.5 40.0 23.0 41.0 54-55 33.18447434131312 36.5 31.5 39.0 22.0 40.5 56-57 32.738969453965865 36.0 31.0 39.0 20.5 40.0 58-59 32.13619500729439 35.5 30.5 39.0 18.5 40.0 60-61 31.572437127032806 35.0 30.0 38.0 15.0 40.0 62-63 31.007053215219536 34.5 29.0 38.0 10.5 40.0 64-65 30.29408479451178 34.0 28.5 37.5 7.5 40.0 66-67 29.585521220470163 34.0 28.0 37.0 4.5 39.0 68-69 28.802526203832155 33.5 26.0 36.0 2.0 39.0 70-71 27.969081434754422 32.5 25.5 35.5 2.0 38.5 72-73 27.367339639463907 32.0 25.0 35.0 2.0 37.5 74-75 26.631940932583703 31.5 23.5 35.0 2.0 37.0 76-77 25.526998494403998 30.5 22.5 34.0 2.0 35.5 78-79 25.998344238766958 31.0 23.0 34.0 2.0 36.0 80-81 25.618489616908786 31.0 21.0 34.0 2.0 36.0 82-83 24.910240479752115 31.0 19.0 34.0 2.0 35.0 84-85 24.116543687918437 30.5 14.5 34.0 2.0 35.0 86-87 23.185793216718153 29.5 7.0 34.0 2.0 35.0 88-89 22.143531261214992 29.0 2.0 34.0 2.0 35.0 90-91 21.23113310433389 28.5 2.0 33.0 2.0 35.0 92-93 20.120566144142238 26.5 2.0 33.0 2.0 34.5 94-95 18.903574357118394 25.0 2.0 32.5 2.0 34.0 96-97 17.484070199665148 22.0 2.0 32.0 2.0 34.0 98-99 15.689951428374675 4.5 2.0 31.5 2.0 34.0 100-101 11.657444448725249 2.0 2.0 25.5 2.0 31.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2650195104568631 1101 2 0.24185601550907165 1101 3 0.2634324368711347 1101 4 0.18652107741634438 1101 5 0.17265488605824686 1101 6 0.22648676846774407 1101 7 0.2527180160898794 1101 8 0.26361370717113175 1101 9 0.31802131274677947 1101 10-11 0.34985544748178654 1101 12-13 0.3128888442825186 1101 14-15 0.499565710510673 1101 16-17 0.3980882066742879 1101 18-19 0.3849504905393957 1101 20-21 0.4211644187492283 1101 22-23 0.41755419708062647 1101 24-25 0.3715240482270801 1101 26-27 0.3946684197642796 1101 28-29 0.41396295819294693 1101 30-31 0.5088666828474331 1101 32-33 0.5001931287547521 1101 34-35 0.3583397696331758 1101 36-37 0.4624111504518922 1101 38-39 0.5781669564518879 1101 40-41 0.6712785273432402 1101 42-43 0.7365148214554935 1101 44-45 0.7074119357738837 1101 46-47 0.7888935190219613 1101 48-49 0.8374356050210494 1101 50-51 0.9231623417014916 1101 52-53 0.9615330414914425 1101 54-55 1.1119402440608255 1101 56-57 1.1393564375185505 1101 58-59 1.244563510245257 1101 60-61 1.2738596324848999 1101 62-63 1.207390044226802 1101 64-65 1.2824049605852998 1101 66-67 1.458851910281556 1101 68-69 1.5033101126528337 1101 70-71 1.90323322141019 1101 72-73 1.9506378253178518 1101 74-75 1.9143146515568255 1101 76-77 1.9895781144754636 1101 78-79 2.0652457348154343 1101 80-81 2.180167858681898 1101 82-83 2.2898035103896177 1101 84-85 2.5823980526812598 1101 86-87 2.537504486131688 1101 88-89 2.307701911097368 1101 90-91 2.569537997830018 1101 92-93 2.947926587287494 1101 94-95 2.8159564171031306 1101 96-97 2.706949875697301 1101 98-99 2.713347882609634 1101 100-101 2.131530682816347 1102 1 0.30630788928155894 1102 2 0.3294691250061774 1102 3 0.32195984005558387 1102 4 0.2570130907456303 1102 5 0.2634584908555979 1102 6 0.2958172396602663 1102 7 0.3414854982779403 1102 8 0.3912248790795516 1102 9 0.43850237888381827 1102 10-11 0.42359714999721376 1102 12-13 0.4502083190158288 1102 14-15 0.6171121754132045 1102 16-17 0.5140970552410664 1102 18-19 0.4885647460448439 1102 20-21 0.5242204225994129 1102 22-23 0.5371777331579182 1102 24-25 0.46671006869014775 1102 26-27 0.5538946876577882 1102 28-29 0.5090909796777581 1102 30-31 0.46631900924986525 1102 32-33 0.5175135447076187 1102 34-35 0.7117570951688492 1102 36-37 0.709675547296527 1102 38-39 0.7970458628577148 1102 40-41 0.8279065605013756 1102 42-43 0.8151022420929479 1102 44-45 0.7550214219433826 1102 46-47 0.7165777089784413 1102 48-49 0.908090309620583 1102 50-51 1.0228903899154602 1102 52-53 1.0465127583939235 1102 54-55 0.9179039269573437 1102 56-57 1.099517895276449 1102 58-59 1.2417262020669995 1102 60-61 1.2468862930802018 1102 62-63 1.4528988000503666 1102 64-65 1.5874037096089246 1102 66-67 1.7281440617053967 1102 68-69 1.85404114795206 1102 70-71 1.9966483887588424 1102 72-73 2.1891726865642553 1102 74-75 2.238841913926006 1102 76-77 2.246982819212871 1102 78-79 2.2760244542825596 1102 80-81 2.6000813345009206 1102 82-83 2.774509106289134 1102 84-85 2.737222234891071 1102 86-87 2.8166172157400844 1102 88-89 2.7188561165012537 1102 90-91 3.0955047194685648 1102 92-93 3.338385773746584 1102 94-95 2.859126499704338 1102 96-97 3.2466852422609556 1102 98-99 3.2186605338688583 1102 100-101 2.4077474618564167 1103 1 0.2815370378845081 1103 2 0.2497211938304602 1103 3 0.24576154100548564 1103 4 0.16404680738121868 1103 5 0.20405846598038835 1103 6 0.2007778431843974 1103 7 0.24956461718074507 1103 8 0.2679030624456118 1103 9 0.21424572244124107 1103 10-11 0.24346460013777715 1103 12-13 0.3495689170359597 1103 14-15 0.5387341679606479 1103 16-17 0.3930786082692279 1103 18-19 0.2640233738480404 1103 20-21 0.36784312030195565 1103 22-23 0.4569032140965703 1103 24-25 0.42180820962538235 1103 26-27 0.5142012103246145 1103 28-29 0.57966948206127 1103 30-31 0.5502357723556415 1103 32-33 0.5280225180117952 1103 34-35 0.5680605670480645 1103 36-37 0.579282433155754 1103 38-39 0.5720587650874123 1103 40-41 0.7489461657002892 1103 42-43 0.5723389275994961 1103 44-45 0.6481258234979421 1103 46-47 0.7749010141937376 1103 48-49 0.796448674232586 1103 50-51 0.956760386906133 1103 52-53 1.0343457949640609 1103 54-55 0.9621537051891025 1103 56-57 1.1830476808421082 1103 58-59 1.3171781107034803 1103 60-61 1.19829885491977 1103 62-63 1.2276988691005322 1103 64-65 1.5027562817988631 1103 66-67 1.447042897502545 1103 68-69 1.2997832066475539 1103 70-71 1.6430342812250132 1103 72-73 1.8796317557595223 1103 74-75 1.7997882853257998 1103 76-77 1.9321434859257636 1103 78-79 2.1655551437191 1103 80-81 2.368010296110892 1103 82-83 2.4714750004422434 1103 84-85 2.5424736210766063 1103 86-87 2.4527888579258708 1103 88-89 2.2880114868667256 1103 90-91 2.7064357940420223 1103 92-93 2.6476339919164964 1103 94-95 2.441730166081989 1103 96-97 2.5260262885781763 1103 98-99 2.6111281335466234 1103 100-101 2.2852622350881138 1104 1 0.19976566418148067 1104 2 0.1919720033711343 1104 3 0.18522934955420567 1104 4 0.173051502061341 1104 5 0.2022360071718765 1104 6 0.10179781956258438 1104 7 0.09426689742436167 1104 8 0.14342837513090956 1104 9 0.23962622899177433 1104 10-11 0.2521192301719992 1104 12-13 0.22668470706550892 1104 14-15 0.22342299440106927 1104 16-17 0.29995623526409076 1104 18-19 0.3570356020366887 1104 20-21 0.2807537753827489 1104 22-23 0.3700330370848306 1104 24-25 0.33640507700843614 1104 26-27 0.37809863007981903 1104 28-29 0.28549377428699074 1104 30-31 0.3466544134317857 1104 32-33 0.4497943300506364 1104 34-35 0.30474395950496813 1104 36-37 0.37913950806838415 1104 38-39 0.3952875087341283 1104 40-41 0.4116886913843558 1104 42-43 0.41731111225828954 1104 44-45 0.4115852353272018 1104 46-47 0.465779047425805 1104 48-49 0.4564220910637218 1104 50-51 0.5894979631920947 1104 52-53 0.683379022889703 1104 54-55 0.8187785038825197 1104 56-57 0.9307700603360018 1104 58-59 0.8436920345959606 1104 60-61 0.9494545773420668 1104 62-63 0.995019260137493 1104 64-65 1.1555304211592805 1104 66-67 1.0989319522917604 1104 68-69 1.2676585843239074 1104 70-71 1.42842705082397 1104 72-73 1.6512218655229525 1104 74-75 1.6254094537703132 1104 76-77 1.7329158025685096 1104 78-79 1.8971897790823746 1104 80-81 1.9336045327887597 1104 82-83 2.069068010470126 1104 84-85 2.314184532048877 1104 86-87 2.3445379023883106 1104 88-89 2.3062596743308745 1104 90-91 2.7602899652387336 1104 92-93 3.0086280409038046 1104 94-95 2.794995785160566 1104 96-97 2.8078744055634424 1104 98-99 3.060596297596044 1104 100-101 2.5694700311149283 1105 1 0.07571892556299531 1105 2 0.09660327535502233 1105 3 0.07698645705102791 1105 4 0.04210670854702414 1105 5 7.426060956063907E-4 1105 6 -0.05413519910680975 1105 7 0.0482124725879558 1105 8 0.03339846201423313 1105 9 0.1548080125550939 1105 10-11 0.08727039913851797 1105 12-13 0.11963232654260736 1105 14-15 0.17159551341618595 1105 16-17 0.1642972447941986 1105 18-19 0.15535217500565324 1105 20-21 0.06392626934500356 1105 22-23 0.12610660230745196 1105 24-25 0.0957584375992866 1105 26-27 0.20724425234280375 1105 28-29 0.23278330519720924 1105 30-31 0.2737945541286493 1105 32-33 0.314683997248423 1105 34-35 0.42617104565674424 1105 36-37 0.4305519884018807 1105 38-39 0.3479061590863708 1105 40-41 0.36461561719782765 1105 42-43 0.36544442354510664 1105 44-45 0.467985489864283 1105 46-47 0.4970308637582619 1105 48-49 0.6244126761983537 1105 50-51 0.638944294952104 1105 52-53 0.6313333314808389 1105 54-55 0.5731926928211806 1105 56-57 0.8230973709614204 1105 58-59 0.9196295126747032 1105 60-61 0.885434369703944 1105 62-63 1.0320825887608898 1105 64-65 1.2398013578356668 1105 66-67 1.2273583068999834 1105 68-69 1.3344191992213439 1105 70-71 1.4486046255065546 1105 72-73 1.518449089966527 1105 74-75 1.312935007689081 1105 76-77 1.407049975673761 1105 78-79 1.486841694624168 1105 80-81 1.5448859616035584 1105 82-83 1.7274231397929967 1105 84-85 1.9402976306870023 1105 86-87 1.9776567832268768 1105 88-89 1.940297368866343 1105 90-91 2.140703888617061 1105 92-93 2.096396858108104 1105 94-95 1.9687071912273701 1105 96-97 2.0953815456289853 1105 98-99 2.081329419483227 1105 100-101 2.0464431944896475 1106 1 -0.020392986326719154 1106 2 0.003158292204972213 1106 3 -0.04511486440166124 1106 4 0.057826216521768004 1106 5 0.055502338915545124 1106 6 -0.06022604435942469 1106 7 0.020599638402885034 1106 8 -0.004975660629206402 1106 9 -0.1166247707609358 1106 10-11 -0.034058310958350546 1106 12-13 0.05563594852582554 1106 14-15 0.006528793998931803 1106 16-17 -0.024743652033038188 1106 18-19 0.07230229209852723 1106 20-21 0.014811101702285612 1106 22-23 0.06474128598870976 1106 24-25 0.03694627506026649 1106 26-27 -0.19234512462904974 1106 28-29 0.10844739145282034 1106 30-31 0.3246638889000266 1106 32-33 -0.011304320343732854 1106 34-35 -0.00596384969885122 1106 36-37 -0.021664040385822148 1106 38-39 0.1167532814912633 1106 40-41 0.16584878824748728 1106 42-43 0.28180508638819646 1106 44-45 0.20510821394509549 1106 46-47 -0.1439963699940492 1106 48-49 0.006350176828341603 1106 50-51 0.18366772394208652 1106 52-53 -0.015034395573501058 1106 54-55 0.06375315104573787 1106 56-57 0.2296369322243379 1106 58-59 0.40729805417127807 1106 60-61 0.5746789190895853 1106 62-63 0.4542409026907457 1106 64-65 0.7189827241095585 1106 66-67 0.635868413509936 1106 68-69 0.6438940620530076 1106 70-71 0.5976956017956674 1106 72-73 0.7895603718730761 1106 74-75 0.721012189866773 1106 76-77 0.8905040137818645 1106 78-79 1.0425193549170864 1106 80-81 1.165878890672495 1106 82-83 1.3818906444212722 1106 84-85 1.4486483515557431 1106 86-87 1.118906065046069 1106 88-89 1.1381012895455846 1106 90-91 1.2899011220669827 1106 92-93 1.3868297237419362 1106 94-95 1.2824464941131986 1106 96-97 1.3538723747540296 1106 98-99 1.8317231589625056 1106 100-101 1.672777414839281 1107 1 -0.06897286627904364 1107 2 0.03542180002325779 1107 3 0.05386022621348019 1107 4 0.06660311096171512 1107 5 -0.019630731871473017 1107 6 -0.029484982221973155 1107 7 0.0095532327789698 1107 8 -0.01243084897416935 1107 9 0.06504954632348614 1107 10-11 0.10634307164884405 1107 12-13 0.08093543758507593 1107 14-15 0.17605951182012092 1107 16-17 0.08113735497427399 1107 18-19 0.058876553633808726 1107 20-21 0.14435225176254107 1107 22-23 0.149089637665476 1107 24-25 0.17039530029894934 1107 26-27 0.27016804660468097 1107 28-29 0.20743545280761566 1107 30-31 0.19771930088180767 1107 32-33 0.34921792009662767 1107 34-35 0.43474137555338643 1107 36-37 0.4407578037472888 1107 38-39 0.40914378635336135 1107 40-41 0.4098605619147122 1107 42-43 0.45281168616239853 1107 44-45 0.4586230798039068 1107 46-47 0.44655960582620224 1107 48-49 0.4177347791446451 1107 50-51 0.5646831192689561 1107 52-53 0.5131476664745875 1107 54-55 0.5130375590218961 1107 56-57 0.594921970979442 1107 58-59 0.6738767871337785 1107 60-61 0.7671209550227047 1107 62-63 0.9578672678099416 1107 64-65 1.1232346062170748 1107 66-67 1.1540185699935215 1107 68-69 1.1906876215611248 1107 70-71 1.1481097161862976 1107 72-73 1.437440511109262 1107 74-75 1.4523766740339603 1107 76-77 1.2938296541435967 1107 78-79 1.321824511166625 1107 80-81 1.4173813278621985 1107 82-83 1.6412995862282322 1107 84-85 1.9097529086471283 1107 86-87 2.064731540920821 1107 88-89 2.0498465766252885 1107 90-91 2.158487480024043 1107 92-93 2.4406064512398693 1107 94-95 2.1866248071674406 1107 96-97 1.9201849334087804 1107 98-99 2.1782156418255827 1107 100-101 1.9382717203163207 1108 1 0.08994262066417136 1108 2 0.09882628125190251 1108 3 0.04839784438061656 1108 4 0.037043794501101956 1108 5 0.06131295485229771 1108 6 0.11872561061161946 1108 7 0.058542727046045684 1108 8 0.09030910377936863 1108 9 0.09686968345682345 1108 10-11 0.03978679994624201 1108 12-13 0.10110609652228675 1108 14-15 0.22083638226447277 1108 16-17 0.1917492339859308 1108 18-19 0.08594145272600429 1108 20-21 0.048155085169653944 1108 22-23 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-0.40610928484392517 2316 64-65 -0.34690035313032297 2316 66-67 -0.2871136715257627 2316 68-69 0.048949238182558474 2316 70-71 -0.1616895940905252 2316 72-73 0.09327560162023119 2316 74-75 0.3738581875346263 2316 76-77 0.3799854470213191 2316 78-79 0.11091203279645612 2316 80-81 -0.27294971494899656 2316 82-83 0.21624501353646153 2316 84-85 0.3290758290425906 2316 86-87 0.43051024190164355 2316 88-89 0.35645223624568345 2316 90-91 0.4015742073780686 2316 92-93 0.5500709634559122 2316 94-95 0.8221913433009114 2316 96-97 0.5750243086865403 2316 98-99 0.3190873122497493 2316 100-101 0.1079097169582095 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 24.0 4 59.0 5 124.0 6 289.0 7 793.0 8 1803.0 9 3173.0 10 5304.0 11 7956.0 12 11465.0 13 15764.0 14 21319.0 15 28076.0 16 35050.0 17 43317.0 18 44781.0 19 43919.0 20 46068.0 21 50444.0 22 54406.0 23 60374.0 24 68239.0 25 77316.0 26 87376.0 27 101750.0 28 119857.0 29 139196.0 30 161413.0 31 185850.0 32 217262.0 33 255431.0 34 305336.0 35 359391.0 36 383338.0 37 287018.0 38 71996.0 39 1081.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.964589833834964 18.322121848694874 14.794911607338197 23.918376710131966 2 16.586200420570517 19.780709884924118 38.06336106291294 25.569728631592426 3 19.93405976001102 22.67202375498966 29.346015766682704 28.04790071831662 4 14.115895398846428 16.124378065683786 34.32076323979506 35.438963295674725 5 14.638523679322354 36.62503788272933 33.43982463064529 15.29661380730303 6 35.06597714030195 34.53615174757438 15.848043711125786 14.549827400997886 7 30.466310052060315 31.72739459690344 19.098316966334778 18.70797838470147 8 28.012596898342935 34.1053347518647 18.57875655228923 19.30331179750313 9 26.84350425530237 16.20012146738916 18.769896959007486 38.18647731830099 10-11 26.141157133803894 25.5685118990016 26.291125174396434 21.999205792798076 12-13 27.009658202707282 22.542743242640615 26.696586456188616 23.75101209846349 14-15 23.11467557181857 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23.974721772934675 40-41 23.741993247507256 25.263746492684398 27.50096481519238 23.493295444615967 42-43 23.58526928648021 25.35077118313307 27.0729353166061 23.99102421378062 44-45 23.410595674906602 25.534372160680995 27.535118441789315 23.519913722623087 46-47 24.042673052688933 25.920361998637286 26.662012045992466 23.37495290268132 48-49 23.643757722512255 26.057337634227235 26.407131604501803 23.891773038758707 50-51 23.818131404584864 26.13515052471371 26.60785245826544 23.438865612435986 52-53 24.328192626492967 25.580661631024924 26.657103619163998 23.43404212331811 54-55 23.45902415657956 25.980859545760804 26.881638554421546 23.67847774323809 56-57 23.590812676254593 26.147293020682156 26.56124862272659 23.700645680336663 58-59 23.49319812193356 25.608146418073947 26.664369189250355 24.234286270742142 60-61 22.943456876338782 25.97505012333267 27.060395847673608 24.021097152654935 62-63 23.315775630701538 25.962672868237746 27.16393401345541 23.557617487605302 64-65 22.921998298731573 26.123116257791917 26.872138513829174 24.08274692964733 66-67 22.794147649043996 26.17148874186771 26.880409106980586 24.15395450210771 68-69 23.66959582579277 25.549939728580668 27.024636577827188 23.75582786779938 70-71 23.250676161161085 25.90289945004598 26.456994390641942 24.389429998150995 72-73 23.216271399927283 26.0347586375944 26.50424612235221 24.244723840126102 74-75 23.253176449602577 26.492498260965323 26.237642084366165 24.016683205065938 76-77 23.319127803956412 25.968861495785212 26.64060061237076 24.071410087887614 78-79 23.36155327270703 26.225932230281256 26.62848121675698 23.78403328025473 80-81 23.708972243980188 26.281198750381478 26.246205670122798 23.763623335515536 82-83 23.478857316903543 26.347396399540763 26.346531801508316 23.827214482047378 84-85 23.172362385547245 26.640852903873867 26.284379058208234 23.90240565237065 86-87 23.70906254626472 26.53441124575578 25.81469901536535 23.94182719261415 88-89 23.229559554770717 26.83787828365089 25.449926874084788 24.482635287493604 90-91 23.024026157622853 26.76269080345257 25.89420821496916 24.31907482395541 92-93 23.29409862428495 26.22328548305653 26.130804643973036 24.351811248685483 94-95 22.926592052188372 25.87458039025697 26.383974597484837 24.81485296006982 96-97 23.238937030250106 25.484878283480743 26.351604803892524 24.924579882376623 98-99 22.884043018242192 26.051331025137053 25.795731606504003 25.26889435011675 100-101 23.112084169038848 25.37477249571612 26.34069967644678 25.172443658798255 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 405.0 1 302.0 2 284.0 3 380.0 4 328.5 5 305.5 6 546.0 7 1227.5 8 1883.0 9 2066.0 10 1885.5 11 1603.5 12 1663.5 13 1844.5 14 2003.5 15 2316.5 16 2595.5 17 2857.5 18 3198.5 19 3616.5 20 4141.0 21 4889.5 22 6002.0 23 7384.5 24 9066.0 25 11305.5 26 14399.0 27 18298.5 28 21665.0 29 24675.5 30 28645.0 31 36198.5 32 48457.5 33 51106.0 34 46480.0 35 47613.0 36 52551.0 37 58468.5 38 64663.0 39 69787.5 40 74552.5 41 82239.0 42 87641.5 43 91197.0 44 96392.0 45 105064.0 46 111686.0 47 116338.0 48 120007.5 49 121014.0 50 122518.0 51 123831.5 52 126559.5 53 133441.5 54 141040.5 55 136523.0 56 126108.5 57 118090.5 58 111574.5 59 100210.5 60 81624.5 61 68381.5 62 59649.5 63 46538.0 64 33252.5 65 25030.5 66 19103.0 67 14966.0 68 11624.0 69 8815.0 70 6799.0 71 4761.0 72 3387.0 73 2583.5 74 1963.0 75 1535.0 76 1165.5 77 833.5 78 546.5 79 341.0 80 199.0 81 126.5 82 83.5 83 53.0 84 34.5 85 17.0 86 9.0 87 4.5 88 2.0 89 2.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023965763541642333 2 0.0112244715321616 3 0.001547156886865518 4 0.0 5 3.640369145565924E-4 6 0.0 7 0.0 8 6.06728190927654E-5 9 3.337005050102097E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0019566984157416843 26-27 0.009373950549832255 28-29 2.123548668246789E-4 30-31 0.0 32-33 2.730276859174443E-4 34-35 0.01020820181235778 36-37 0.001167951767535734 38-39 0.012437927914016909 40-41 0.012164900228099464 42-43 0.005081348599019103 44-45 7.280738291131848E-4 46-47 9.10092286391481E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.03364095463827E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.516820477319135E-4 66-67 5.6122357660808E-4 68-69 9.252604911646724E-4 70-71 7.584102386595674E-5 72-73 2.5785948114425295E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.10092286391481E-5 82-83 0.0011982881770821168 84-85 0.005900431656771436 86-87 0.0036707055551123066 88-89 0.0 90-91 0.0013651384295872216 92-93 3.1853230023701836E-4 94-95 7.584102386595674E-5 96-97 1.516820477319135E-5 98-99 0.0 100-101 7.584102386595674E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3296369.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.14728947815189 #Duplication Level Percentage of deduplicated Percentage of total 1 69.44406942891527 29.2687929676137 2 15.006754175680747 12.649880247397622 3 5.924210485925049 7.490682428393577 4 2.9117320215120137 4.9088644957388485 5 1.6892901426692986 3.5599500327835716 6 1.0639357319321976 2.690520436793744 7 0.7269004134648814 2.1445817503664864 8 0.49744250882859614 1.6772682734669573 9 0.3865861154181387 1.4664201223286277 >10 2.113276368419615 16.111331135464027 >50 0.14672515119933072 4.23252860663506 >100 0.07673679268289668 5.851114200149556 >500 0.0071445945727219035 2.0367019996735496 >1k 0.004618727803892143 4.361237198100168 >5k 5.773409754865179E-4 1.550126105094599 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7834 0.23765543238636208 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6697 0.20316293473212496 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6555 0.1988551645765386 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6541 0.19843045484288926 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6320 0.19172610833313866 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5890 0.1786814522281941 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5779 0.17531411076854564 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5347 0.16220878184450832 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4748 0.14403727252622509 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4687 0.1421867515438957 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4475 0.1357554327200626 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4318 0.13099261642128052 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4234 0.12844435801938436 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 4224 0.12814099392392053 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3682 0.11169865994978112 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3519 0.10675382519372073 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3434 0.1041752303822782 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3376 0.102415718628588 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3332 0.10108091660854716 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3297 0.10001914227442377 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.100922863914811E-5 2 0.0 0.0 0.0 0.0 9.100922863914811E-5 3 0.0 0.0 0.0 0.0 9.100922863914811E-5 4 0.0 0.0 0.0 0.0 9.100922863914811E-5 5 0.0 0.0 0.0 0.0 9.100922863914811E-5 6 0.0 0.0 0.0 0.0 1.2134563818553081E-4 7 0.0 0.0 0.0 0.0 1.5168204773191352E-4 8 0.0 0.0 0.0 0.0 1.5168204773191352E-4 9 0.0 0.0 0.0 0.0 1.5168204773191352E-4 10-11 0.0 0.0 0.0 0.0 1.8201845727829622E-4 12-13 0.0 0.0 0.0 0.0 6.825692147936108E-4 14-15 0.0 0.0 0.0 0.0 9.100922863914811E-4 16-17 0.0 0.0 0.0 3.0336409546382703E-5 0.0012134563818553081 18-19 0.0 0.0 0.0 3.0336409546382703E-5 0.0012892974057212648 20-21 0.0 0.0 0.0 3.0336409546382703E-5 0.001516820477319135 22-23 0.0 0.0 0.0 9.100922863914811E-5 0.0016078297059582832 24-25 0.0 0.0 0.0 3.1853230023701836E-4 0.0016533343202778572 26-27 0.0 0.0 0.0 8.039148529791416E-4 0.0017898481632365793 28-29 0.0 0.0 0.0 0.002639267630535295 0.0018505209823293449 30-31 0.0 0.0 0.0 0.010390220269636076 0.0018656891871025362 32-33 1.5168204773191352E-5 0.0 0.0 0.02567977068101296 0.002032539439607641 34-35 3.0336409546382703E-5 0.0 0.0 0.05011574857062422 0.0020628758491540236 36-37 3.0336409546382703E-5 0.0 0.0 0.09044800506254003 0.0020628758491540236 38-39 3.0336409546382703E-5 0.0 0.0 0.15424547433858285 0.002123548668246789 40-41 3.0336409546382703E-5 0.0 0.0 0.22682533417830347 0.002123548668246789 42-43 3.0336409546382703E-5 0.0 0.0 0.30121021038603385 0.002123548668246789 44-45 3.0336409546382703E-5 0.0 0.0 0.39337222258794446 0.002214557896885937 46-47 4.5504614319574055E-5 0.0 0.0 0.5027804836169737 0.002335903535071468 48-49 6.067281909276541E-5 0.0 0.0 0.6188779229509803 0.0024724173780301902 50-51 6.067281909276541E-5 0.0 0.0 0.7499312121913536 0.002548258401896147 52-53 6.067281909276541E-5 0.0 0.0 0.8750689015701822 0.002669604040081678 54-55 6.067281909276541E-5 0.0 0.0 1.0125535096343885 0.002730276859174443 56-57 6.067281909276541E-5 0.0 0.0 1.1722747058960934 0.002775781473494017 58-59 6.067281909276541E-5 0.0 0.0 1.3453135859486605 0.0028364542925867825 60-61 6.067281909276541E-5 0.0 0.0 1.5331718020646354 0.002927463521225931 62-63 6.067281909276541E-5 0.0 0.0 1.7207266540851465 0.003185323002370184 64-65 6.067281909276541E-5 0.0 0.0 1.9121038937085015 0.003185323002370184 66-67 9.100922863914811E-5 0.0 0.0 2.1435555303426286 0.003185323002370184 68-69 9.100922863914811E-5 0.0 0.0 2.4011571520057373 0.0032156594119165664 70-71 1.0617743341233946E-4 0.0 0.0 2.6680568831948124 0.003245995821462949 72-73 1.3651384295872217E-4 0.0 0.0 2.9511410888768825 0.0035796963264731587 74-75 1.5168204773191352E-4 0.0 0.0 3.232844987924592 0.003883060421936986 76-77 1.5168204773191352E-4 0.0 0.0 3.542246028888149 0.003943733241029751 78-79 1.5168204773191352E-4 0.0 0.0 3.8891428720510355 0.004049910674442091 80-81 1.8201845727829622E-4 0.0 0.0 4.244549078091682 0.004095415288761665 82-83 1.8201845727829622E-4 0.0 0.0 4.623739029216693 0.004398779384225492 84-85 1.9718666205148757E-4 0.0 0.0 5.015533758508225 0.004429115793771875 86-87 2.1235486682467892E-4 0.0 0.0 5.41242500460355 0.00448978861286464 88-89 2.1235486682467892E-4 0.0 0.0 5.825015342639128 0.004565629636730597 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4490 0.0 52.488697 1 GTATCAA 7955 0.0 44.2597 1 TATCAAC 11095 0.0 31.47011 2 TCAACGC 11165 0.0 31.013279 4 ATCAACG 11180 0.0 30.97167 3 CAACGCA 11605 0.0 29.878351 5 AGGTAAG 2025 0.0 29.324 2 AACGCAG 12155 0.0 28.525955 6 TAAGGTG 2390 0.0 28.41954 5 GTAAGGT 2460 0.0 26.838524 4 AAGGTAA 2200 0.0 25.485302 1 ACGCAGA 13985 0.0 24.691319 7 GTGGTAT 2215 0.0 24.454657 1 CGCAGAG 14180 0.0 24.351768 8 TACATGG 10495 0.0 23.80902 2 TGGTATC 2130 0.0 23.640924 2 GTACATG 10870 0.0 23.604492 1 GTGTAAG 1120 0.0 23.333246 1 GGTAAGG 2720 0.0 22.876064 3 ACATGGG 10430 0.0 22.587921 3 >>END_MODULE