FastQCFastQC Report
Thu 26 May 2016
SRR938375_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938375_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3176642
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93030.29285641882213986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73930.23273003379039878No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA63360.19945590343513683No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA63120.1987003886493977No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA59100.18604551598826685No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA55560.17490167289861433No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC52440.1650799806840053No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50000.15739891369565723No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA49840.15689523717183113No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG46770.14723094387091776No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA45610.14357928907317855No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA45030.1417534616743089No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT39580.12459698008148226No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG38690.12179527941769956No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA34680.10917188653930786No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA34320.10803861436069913No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG33560.10564615087252514No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT32960.10375736390817725No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG32680.10287592999148155No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT32620.10268705129504677No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58000.050.5061651
GTATCAA105800.044.9924931
TATCAAC139600.033.895042
ATCAACG141300.033.3526233
AGGTAAG19850.033.3006552
TCAACGC144300.032.659224
TAAGGTG22400.032.4819345
CAACGCA146550.032.1578035
AAGGTAA20350.031.5472151
AACGCAG151800.031.148346
GTAAGGT24750.028.6292254
ACGCAGA171750.027.2863797
CGCAGAG174750.026.7907438
TGGTATC27050.026.722362
GTGTAAG9650.026.6108111
TAAGGTA10100.026.3672684
GGTAAGG27300.025.2582863
TACATGG137900.025.2432542
GTACATG141250.025.115511
ACATGGG140100.023.7945983
Produced by FastQC (version 0.11.5)