##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938375_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3176642 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.130539418669148 31.0 31.0 34.0 28.0 34.0 2 31.155921252693883 31.0 31.0 34.0 27.0 34.0 3 31.201799258462238 31.0 31.0 34.0 27.0 34.0 4 34.86876361894101 37.0 35.0 37.0 32.0 37.0 5 34.848143102055566 37.0 35.0 37.0 32.0 37.0 6 34.873114754511214 37.0 35.0 37.0 32.0 37.0 7 34.772845980126185 37.0 35.0 37.0 32.0 37.0 8 34.72956064926422 37.0 35.0 37.0 31.0 37.0 9 36.22278966279487 39.0 35.0 39.0 32.0 39.0 10-11 36.06107754666721 39.0 35.0 39.0 30.0 39.0 12-13 35.92150264335736 38.0 35.0 39.0 30.0 39.0 14-15 36.894113186188434 39.0 36.0 41.0 30.5 41.0 16-17 36.72270403778582 39.0 36.0 41.0 30.0 41.0 18-19 36.857142070148285 39.0 36.0 41.0 30.0 41.0 20-21 36.819749439817265 39.0 36.0 41.0 30.0 41.0 22-23 36.743593392015846 39.0 36.0 41.0 30.0 41.0 24-25 36.593947319213186 39.0 36.0 41.0 30.0 41.0 26-27 36.37893410714837 39.0 35.5 41.0 29.5 41.0 28-29 36.171167698469006 39.0 35.0 40.5 29.0 41.0 30-31 35.8956505013785 38.5 35.0 40.0 27.5 41.0 32-33 35.61146975328035 38.0 34.5 40.0 27.0 41.0 34-35 35.35893248279157 38.0 34.0 40.0 26.5 41.0 36-37 35.06845609294343 38.0 34.0 40.0 25.0 41.0 38-39 34.92146612680938 38.0 34.0 40.0 25.0 41.0 40-41 34.79569731181543 38.0 33.5 40.0 24.5 41.0 42-43 34.4754057901394 38.0 33.0 40.0 24.0 41.0 44-45 34.12005633621919 38.0 33.0 40.0 23.0 41.0 46-47 33.71277027754465 38.0 33.0 40.0 21.5 41.0 48-49 33.30920717537576 37.5 32.0 40.0 19.0 41.0 50-51 32.56090220427735 36.5 31.0 39.5 17.5 40.5 52-53 32.54057980093445 36.5 31.0 39.0 17.0 40.0 54-55 32.616194239073835 37.0 31.0 40.0 14.5 41.0 56-57 32.273214923179886 36.0 31.0 39.0 12.0 41.0 58-59 31.8720918189711 36.0 31.0 39.0 8.5 41.0 60-61 31.369051344155245 35.0 30.0 39.0 7.5 40.0 62-63 30.78073103610668 35.0 29.0 38.0 4.0 40.0 64-65 30.236361384128273 34.5 29.0 38.0 2.0 40.0 66-67 29.690582539675546 34.0 28.0 37.0 2.0 39.5 68-69 29.10559452402883 34.0 27.5 36.5 2.0 39.0 70-71 28.45802470029673 34.0 26.0 36.0 2.0 39.0 72-73 27.835727160945424 33.0 26.0 35.5 2.0 38.5 74-75 27.184442250653362 33.0 25.0 35.0 2.0 37.5 76-77 26.45853719115972 32.0 23.0 35.0 2.0 37.0 78-79 25.70189007763544 31.5 20.5 35.0 2.0 36.0 80-81 25.045385661966314 31.0 18.5 34.5 2.0 36.0 82-83 24.335929733347353 31.0 14.5 34.0 2.0 35.0 84-85 23.65909032242223 30.5 7.5 34.0 2.0 35.0 86-87 22.91861028091928 30.0 2.0 34.0 2.0 35.0 88-89 22.099083088368157 29.0 2.0 34.0 2.0 35.0 90-91 21.201047836048254 29.0 2.0 34.0 2.0 35.0 92-93 20.27030619125479 27.0 2.0 33.5 2.0 35.0 94-95 19.267178360041832 25.5 2.0 33.0 2.0 35.0 96-97 18.027058762051247 24.0 2.0 32.5 2.0 34.0 98-99 16.515453740144466 10.5 2.0 32.0 2.0 34.0 100-101 13.032329107277434 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39086262207193556 1101 2 0.3519774975327934 1101 3 0.35120683136021924 1101 4 0.3103588571828624 1101 5 0.26265916124950195 1101 6 0.5287575042970403 1101 7 0.4011016975557453 1101 8 0.3732778723449002 1101 9 0.6473754130232834 1101 10-11 0.5913627579513019 1101 12-13 0.7064624626282026 1101 14-15 0.7925525873564752 1101 16-17 0.6667475238590583 1101 18-19 0.696298579055032 1101 20-21 0.7167165086628557 1101 22-23 0.7148506123856535 1101 24-25 0.7220968056579338 1101 26-27 0.5176626123500085 1101 28-29 0.6242329909507376 1101 30-31 0.6542676537915568 1101 32-33 0.7540409525928808 1101 34-35 0.5773141303066396 1101 36-37 0.6584646185576588 1101 38-39 0.7391422238842722 1101 40-41 0.8015131550916692 1101 42-43 0.7805893979529799 1101 44-45 0.8860680154450549 1101 46-47 0.8289428956451204 1101 48-49 0.7050584951296059 1101 50-51 0.7287159778155896 1101 52-53 0.7147485681004326 1101 54-55 0.8583047032800906 1101 56-57 0.6419459709037767 1101 58-59 0.6833342477905191 1101 60-61 0.7389419262019139 1101 62-63 0.6931770447600485 1101 64-65 0.5657922500044172 1101 66-67 0.662768875951695 1101 68-69 0.633685266615938 1101 70-71 0.7061191988963849 1101 72-73 0.7143621105481763 1101 74-75 0.5253860533776376 1101 76-77 0.5463145402070246 1101 78-79 0.7863151888267055 1101 80-81 0.7704968620354187 1101 82-83 0.6108979335643205 1101 84-85 0.6080472877718499 1101 86-87 0.7422292000947017 1101 88-89 0.8881340720518551 1101 90-91 0.7360223421112977 1101 92-93 0.6228481873961336 1101 94-95 0.8132978237549437 1101 96-97 0.6906672248691841 1101 98-99 0.534264108006564 1101 100-101 0.39012774643674497 1102 1 0.3418481496015531 1102 2 0.239894831643241 1102 3 0.18951337104087074 1102 4 0.17955435463652236 1102 5 0.16158834322175153 1102 6 0.17162796730348617 1102 7 0.24843919239717138 1102 8 0.3193188402451099 1102 9 0.44288301368432315 1102 10-11 0.44342504321693355 1102 12-13 0.35484788287183733 1102 14-15 0.5803852187524043 1102 16-17 0.6253595749083729 1102 18-19 0.537236162002138 1102 20-21 0.39047506323485237 1102 22-23 0.5030818229538809 1102 24-25 0.4335541316384024 1102 26-27 0.4109994352619424 1102 28-29 0.2273475635918203 1102 30-31 0.3640294405547877 1102 32-33 0.4272854130415453 1102 34-35 0.34265246784417513 1102 36-37 0.38860993723228887 1102 38-39 0.3626282015324023 1102 40-41 0.46204459811285403 1102 42-43 0.5739277057919665 1102 44-45 0.6193425704155118 1102 46-47 0.7513062065622407 1102 48-49 0.8546298152483232 1102 50-51 0.7562346461330023 1102 52-53 0.5506457517506433 1102 54-55 0.6032661910780064 1102 56-57 0.7212639067809192 1102 58-59 0.8344465509680745 1102 60-61 0.7283851466127267 1102 62-63 0.7392526609583712 1102 64-65 0.9443752088577106 1102 66-67 0.5683090150769878 1102 68-69 0.5873148923957565 1102 70-71 0.7362852446779229 1102 72-73 0.7760794534444422 1102 74-75 0.6221549758341283 1102 76-77 0.6624743208337982 1102 78-79 0.5693231684535043 1102 80-81 0.6873477524657474 1102 82-83 0.6524762006668112 1102 84-85 0.6988034311935287 1102 86-87 0.8450382489927328 1102 88-89 0.8658622697649463 1102 90-91 0.9377884772359479 1102 92-93 1.0602848691781226 1102 94-95 1.072751952192469 1102 96-97 1.114498262443405 1102 98-99 1.0535358626520654 1102 100-101 0.9298730566967865 1103 1 0.45544557123906415 1103 2 0.35118457848429685 1103 3 0.1878347740733517 1103 4 0.2536006052927675 1103 5 0.26993065919037207 1103 6 0.36574195324903513 1103 7 0.25173173013303796 1103 8 0.2566327189857489 1103 9 0.40525850356357296 1103 10-11 0.6344354729307113 1103 12-13 0.5809876331979922 1103 14-15 0.6746658088206061 1103 16-17 0.7217232445548589 1103 18-19 0.6771640286831584 1103 20-21 0.5969211924172342 1103 22-23 0.7004151830415069 1103 24-25 0.6161437661349112 1103 26-27 0.7336808064615141 1103 28-29 0.7955711186783176 1103 30-31 0.8370170544922715 1103 32-33 0.7962500619419544 1103 34-35 0.7001735562086608 1103 36-37 0.8593958374401964 1103 38-39 0.8202258799172171 1103 40-41 0.888519117351187 1103 42-43 0.7694086247032388 1103 44-45 0.7755572280580054 1103 46-47 0.6254638864865996 1103 48-49 0.6845409464173287 1103 50-51 0.7379328934219629 1103 52-53 0.7795466503427377 1103 54-55 0.6676322587536845 1103 56-57 0.6837340340914295 1103 58-59 0.6546508622105804 1103 60-61 0.7774999199323211 1103 62-63 0.8407398943512945 1103 64-65 0.9449612216683185 1103 66-67 0.9074766023024239 1103 68-69 1.0094582090217408 1103 70-71 1.0381402204711563 1103 72-73 1.006122513769025 1103 74-75 1.0148506793534189 1103 76-77 0.9564841880034471 1103 78-79 0.9554036392538947 1103 80-81 1.189883425438211 1103 82-83 1.0353708575301432 1103 84-85 1.1839861899784978 1103 86-87 1.305343097691356 1103 88-89 1.2467055884327038 1103 90-91 1.1941359323749907 1103 92-93 1.0873758166296454 1103 94-95 1.127982192800367 1103 96-97 1.296091651693203 1103 98-99 1.5560509418623667 1103 100-101 1.2880471022668516 1104 1 0.34537198643357314 1104 2 0.28953037485740296 1104 3 0.3126665636219492 1104 4 0.1931894149265787 1104 5 0.3465407299232268 1104 6 0.3563917117762685 1104 7 0.27877286277128377 1104 8 0.3260797777215245 1104 9 0.4606908400731058 1104 10-11 0.513120801030496 1104 12-13 0.5032112228956791 1104 14-15 0.6022224431461112 1104 16-17 0.6589492101497569 1104 18-19 0.6918171498837538 1104 20-21 0.709182335770052 1104 22-23 0.7353758633685956 1104 24-25 0.6522206574522045 1104 26-27 0.6455949108667269 1104 28-29 0.6445600351068421 1104 30-31 0.6431746648881287 1104 32-33 0.7260494291591328 1104 34-35 0.6216731883065734 1104 36-37 0.77097264341824 1104 38-39 0.929743333657413 1104 40-41 0.8418002686982504 1104 42-43 0.8690735639631981 1104 44-45 0.7771342490295226 1104 46-47 0.740889382693716 1104 48-49 0.5445208554970336 1104 50-51 0.6436076899130896 1104 52-53 0.6607524477951898 1104 54-55 0.8494858008149677 1104 56-57 0.9204738731271362 1104 58-59 0.8349860677421432 1104 60-61 0.906369089386228 1104 62-63 0.9053408247903754 1104 64-65 1.0282342252016683 1104 66-67 0.9510942798552193 1104 68-69 0.9429394490865164 1104 70-71 1.2111467812820607 1104 72-73 1.3568371915486637 1104 74-75 1.2732158997283065 1104 76-77 1.112004978968809 1104 78-79 1.2477523068867171 1104 80-81 1.268135673326558 1104 82-83 1.3212129013896892 1104 84-85 1.4437817750656734 1104 86-87 1.6502538293316817 1104 88-89 1.7949327917757216 1104 90-91 1.630252597484052 1104 92-93 1.7561483223873005 1104 94-95 1.7132602298134643 1104 96-97 1.9275236310503203 1104 98-99 1.8717866209938236 1104 100-101 1.8588605761269363 1105 1 0.5231971962365733 1105 2 0.3130736272563759 1105 3 0.34639619779141384 1105 4 0.2726382625995214 1105 5 0.3209741698913078 1105 6 0.4076618485608563 1105 7 0.46788449113592634 1105 8 0.40143712534075604 1105 9 0.553547913967023 1105 10-11 0.6328239595432308 1105 12-13 0.731130629126433 1105 14-15 0.7999750607920149 1105 16-17 0.6236397238885161 1105 18-19 0.6148953054949047 1105 20-21 0.7456265238473776 1105 22-23 0.806545675976146 1105 24-25 0.8393883739298929 1105 26-27 0.8746001797450944 1105 28-29 0.8212551650970923 1105 30-31 0.8663792168835087 1105 32-33 1.0150309429946347 1105 34-35 1.0477772667828447 1105 36-37 0.9780453767005142 1105 38-39 0.9817870397316995 1105 40-41 1.0641081006931543 1105 42-43 1.1265937687939527 1105 44-45 1.1278569454692828 1105 46-47 1.1334378197478898 1105 48-49 1.0384289010490235 1105 50-51 1.2188010077699047 1105 52-53 1.1946589506251968 1105 54-55 1.0526943852514705 1105 56-57 1.0461932365889481 1105 58-59 1.132429185774349 1105 60-61 1.2151936338261713 1105 62-63 1.225350463207974 1105 64-65 1.3241431462638111 1105 66-67 1.4513422246557646 1105 68-69 1.379728572136365 1105 70-71 1.311913694886961 1105 72-73 1.4099391309617992 1105 74-75 1.456014950019604 1105 76-77 1.618673964854029 1105 78-79 1.5786440412862746 1105 80-81 1.69082635099236 1105 82-83 1.7894337526230153 1105 84-85 1.806018960253322 1105 86-87 2.086968024572087 1105 88-89 2.0037001568989865 1105 90-91 1.9649965148763187 1105 92-93 1.8514485872383624 1105 94-95 2.116885400545133 1105 96-97 2.36929327081549 1105 98-99 2.383320111310937 1105 100-101 1.9678972294349855 1106 1 0.21999055689845903 1106 2 0.15103889854435337 1106 3 0.10558789754911757 1106 4 0.06877093270945522 1106 5 0.23233590203015098 1106 6 -0.013897836449395129 1106 7 0.27271694811303604 1106 8 0.1560934079253471 1106 9 0.1383937173514198 1106 10-11 0.05687103504426716 1106 12-13 0.08907519764019156 1106 14-15 0.3444694760582294 1106 16-17 0.19742030011303768 1106 18-19 -0.011492678984680538 1106 20-21 0.09748034345169998 1106 22-23 0.11631885668050046 1106 24-25 0.015434559805001413 1106 26-27 -0.04117870259773326 1106 28-29 0.22786982053404614 1106 30-31 -0.07422977526092467 1106 32-33 0.1664821907162093 1106 34-35 -0.31817771931523 1106 36-37 -0.030443002913862927 1106 38-39 -0.08202722340605817 1106 40-41 0.14743131776057083 1106 42-43 -0.3405884591115438 1106 44-45 -0.12852334055715886 1106 46-47 0.0380478399672981 1106 48-49 0.22946905803329543 1106 50-51 0.0015100741541047569 1106 52-53 0.031263546306050216 1106 54-55 0.05883866711919694 1106 56-57 -0.6056549421249535 1106 58-59 -0.4276345411508551 1106 60-61 0.06602497897268123 1106 62-63 0.08847995857633606 1106 64-65 0.07934681722209191 1106 66-67 -0.4776858882706243 1106 68-69 -0.3534499949422454 1106 70-71 -0.26994919756992175 1106 72-73 -0.047109020344844765 1106 74-75 0.37405432585659426 1106 76-77 0.2655564445208398 1106 78-79 0.4101884353461642 1106 80-81 0.4136295142906903 1106 82-83 0.1305415750647363 1106 84-85 0.11321282110779407 1106 86-87 0.06875652589446801 1106 88-89 0.13308482657338772 1106 90-91 0.2541325635667917 1106 92-93 0.31579437563756585 1106 94-95 0.5007623734314208 1106 96-97 0.6437106823773782 1106 98-99 0.8483644013826748 1106 100-101 0.5024797727661579 1107 1 0.23663116061215916 1107 2 0.048393444497097704 1107 3 0.11094148022747063 1107 4 -0.01484917672407704 1107 5 0.03950330414549086 1107 6 0.22628041176511005 1107 7 0.18187970983924373 1107 8 0.057876565443095274 1107 9 0.2943457574014019 1107 10-11 0.31662770984885924 1107 12-13 0.3423531671380502 1107 14-15 0.4181107613194328 1107 16-17 0.5714618980524904 1107 18-19 0.4004165821884911 1107 20-21 0.5630793681363784 1107 22-23 0.5043090228684974 1107 24-25 0.4234359699831387 1107 26-27 0.38281008119887616 1107 28-29 0.43168913982014345 1107 30-31 0.5810929139418377 1107 32-33 0.6094774569326802 1107 34-35 0.5637856141235886 1107 36-37 0.4921063880543599 1107 38-39 0.560512204850454 1107 40-41 0.4543126030680895 1107 42-43 0.5849960373421723 1107 44-45 0.5012530879332218 1107 46-47 0.5046072445896286 1107 48-49 0.4670418632071929 1107 50-51 0.6150155207452457 1107 52-53 0.7844653916557434 1107 54-55 0.7553644439714375 1107 56-57 0.5556847869232513 1107 58-59 0.47488443489154974 1107 60-61 0.49786202737027097 1107 62-63 0.7840066662850802 1107 64-65 0.6543872334523435 1107 66-67 0.8077335490320223 1107 68-69 0.9445228836350097 1107 70-71 0.9022183473385041 1107 72-73 0.9722493794773222 1107 74-75 0.9564279385446461 1107 76-77 0.8550651282696755 1107 78-79 1.0365746377526506 1107 80-81 1.228929464660446 1107 82-83 1.1585523188931823 1107 84-85 1.1564724667802757 1107 86-87 1.1648319546017412 1107 88-89 1.2176892916743753 1107 90-91 1.1657260880164415 1107 92-93 1.362775807134323 1107 94-95 1.3243378098572407 1107 96-97 1.2977425896838284 1107 98-99 1.516982187534218 1107 100-101 1.3310948883011946 1108 1 0.28343887524661326 1108 2 0.18661287484606248 1108 3 0.2280412379495722 1108 4 0.2805965984217309 1108 5 0.24268473740355745 1108 6 0.2948075159151742 1108 7 0.27628421607570885 1108 8 0.32755667842273084 1108 9 0.3435911496701394 1108 10-11 0.4113368568143514 1108 12-13 0.3114306386779333 1108 14-15 0.4213746705943393 1108 16-17 0.5196597768517393 1108 18-19 0.6153130034209511 1108 20-21 0.4458414759614513 1108 22-23 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-1.4428000482054273 2316 56-57 -1.342974641405661 2316 58-59 -1.1569064651459868 2316 60-61 -1.4229676993715685 2316 62-63 -1.4087023053235725 2316 64-65 -1.6373824978818554 2316 66-67 -1.6594498008346577 2316 68-69 -1.6377280422538867 2316 70-71 -1.8982885534523888 2316 72-73 -1.8595766566088052 2316 74-75 -1.7000036675807344 2316 76-77 -1.871710249987057 2316 78-79 -1.9787079308972864 2316 80-81 -2.133620860997027 2316 82-83 -2.027735414868772 2316 84-85 -2.0910296326752196 2316 86-87 -2.0888561211360326 2316 88-89 -2.472294257753454 2316 90-91 -2.367246328169813 2316 92-93 -2.6322461291118913 2316 94-95 -3.0005726810992996 2316 96-97 -3.1399842869584855 2316 98-99 -3.2150373145046096 2316 100-101 -3.088882640107567 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11190.0 3 3967.0 4 4219.0 5 4945.0 6 5885.0 7 7607.0 8 9360.0 9 11925.0 10 14870.0 11 18232.0 12 22116.0 13 25723.0 14 28917.0 15 33517.0 16 38299.0 17 44533.0 18 42837.0 19 41088.0 20 43693.0 21 46943.0 22 51077.0 23 57207.0 24 63648.0 25 71249.0 26 79668.0 27 89786.0 28 102159.0 29 117017.0 30 135185.0 31 156697.0 32 184183.0 33 219391.0 34 265892.0 35 322032.0 36 361879.0 37 316330.0 38 118570.0 39 4806.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.64222694518802 17.423053033253254 14.315738904012385 23.618981117546337 2 16.32773389418062 19.240038899641824 38.72316904768358 25.70905815849398 3 19.850634034790918 22.827206452418082 28.780256740283367 28.541902772507637 4 13.48398603318238 15.54307995495673 34.73141232336227 36.241521688498615 5 14.688588640758363 36.68751475956065 32.94488429292679 15.679012306754196 6 34.43492065092583 34.224211708447136 16.357876865563473 14.982990775063557 7 30.72820151754701 31.815240524935113 18.9572146459629 18.499343311554977 8 28.317838938059786 33.13288863306308 18.77511987636392 19.774152552513215 9 27.328221536926918 16.174987924766416 18.796840728456175 37.699949809850494 10-11 26.27212304021352 25.649183455762735 25.82642604299113 22.252267461032616 12-13 27.323910612765555 22.68364856139467 26.24509047386573 23.74735035197405 14-15 23.627898657911174 24.164038291954505 24.4384609220128 27.76960212812152 16-17 23.563336205180914 27.393253551392217 25.457307088677112 23.58610315474976 18-19 23.83489149303847 25.526379807824785 27.608863206043168 23.029865493093578 20-21 23.97368413178156 24.701947153132885 28.01587305337052 23.30849566171504 22-23 23.91358119353857 24.49386736581473 28.094834656385597 23.497716784261108 24-25 23.732528109350888 24.856366728606975 28.001496015873517 23.409609146168624 26-27 22.805857660721713 25.332527416247252 27.842771204065954 24.01884371896508 28-29 23.293551154724664 25.86435843520351 27.59652417230349 23.245566237768333 30-31 23.05869974901579 25.247121399337374 27.435747806152307 24.25843104549453 32-33 22.760681581571856 25.233157268224993 27.491321807974128 24.514839342229028 34-35 23.98100906471455 25.364994341936058 27.19556111693411 23.458435476415286 36-37 24.12604812806265 24.620780951865008 27.251660343199536 24.001510576872803 38-39 23.33831346084538 25.081846183495106 27.253861960564258 24.32597839509526 40-41 23.833309006030497 24.893381068382713 27.57963491410415 23.693675011482647 42-43 23.477924197178407 24.927989555297124 27.25252481356955 24.34156143395492 44-45 23.323699785921168 25.307911472106788 27.523312555093817 23.845076186878227 46-47 24.22111660247828 25.714962624333026 26.475132607465373 23.588788165723322 48-49 23.716462973107415 25.793932639452954 26.348423027761513 24.14118135967812 50-51 23.98018743420158 25.903437511018872 26.50004659409741 23.616328460682137 52-53 24.447887976216542 25.26595589778189 26.605805985564135 23.680350140437433 54-55 23.544387726911452 25.85559177732725 26.718302762063573 23.881717733697723 56-57 23.583603810492928 26.088942776173994 26.445503509407693 23.881949903925378 58-59 23.57219199477584 25.257055739844937 26.477359117018867 24.693393148360357 60-61 22.8867750157832 25.81541290585655 26.961895180652707 24.335916897707538 62-63 23.348245010323467 25.767380987312745 27.17871155302875 23.705662449335037 64-65 23.018533554425886 25.936980436961075 26.778765040469445 24.265720968143594 66-67 22.882381137157335 25.905665322596516 26.840936656594668 24.371016883651482 68-69 23.7363925065871 25.21884857237217 27.16091418806196 23.88384473297877 70-71 23.507877687851224 25.562667247749932 26.416091303967214 24.51336376043163 72-73 23.382473131027442 25.772391938812284 26.392516001411327 24.452618928748944 74-75 23.37130824673544 26.325828724627982 26.299629718615748 24.003233310020835 76-77 23.74377241007435 25.70843583092915 26.432705818452323 24.11508594054418 78-79 23.75135960459314 26.035794394730004 26.433357731447593 23.779488269229258 80-81 24.052481943079815 26.052492646741694 26.02930662622781 23.865718783950676 82-83 23.81689203930308 26.15159472403566 26.120900013001965 23.910613223659293 84-85 23.595474667111187 26.360632317568523 26.19282043435905 23.851072580961244 86-87 24.162788753719248 26.314894664702276 25.65692587827428 23.8653907033042 88-89 23.825571887384648 26.47803280722586 25.450675397036964 24.245719908352527 90-91 23.675872076545478 26.428362689118394 25.60542763965884 24.29033759467729 92-93 23.950131026973104 25.870474436672186 25.89462672899868 24.284767807356026 94-95 23.73049318604956 25.626283309352765 26.188242693512304 24.454980811085367 96-97 24.22867067788043 25.20731340533782 26.035716703960144 24.528299212821608 98-99 23.788636835239245 25.70831245947901 25.61380152483309 24.889249180448658 100-101 24.53073538508406 24.83149948720146 26.024333003543987 24.613432124170494 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 465.0 1 337.0 2 314.0 3 437.5 4 396.5 5 401.5 6 669.5 7 1395.5 8 2240.5 9 2585.5 10 2306.5 11 1956.5 12 1933.5 13 2112.0 14 2354.5 15 2561.0 16 2794.5 17 3089.5 18 3485.5 19 3885.5 20 4335.5 21 5053.5 22 6042.5 23 7146.0 24 8491.5 25 10431.5 26 13018.0 27 16088.0 28 18899.5 29 21610.5 30 24934.5 31 32002.0 32 43492.0 33 45800.5 34 41509.0 35 43041.0 36 47188.0 37 52607.0 38 59448.5 39 64699.5 40 69311.0 41 76391.5 42 81887.5 43 87083.5 44 92804.0 45 100481.0 46 106563.5 47 111514.5 48 115102.0 49 114539.5 50 115823.0 51 117761.0 52 121312.0 53 128604.0 54 136012.0 55 132613.0 56 124844.0 57 119806.5 58 115547.0 59 104789.5 60 84832.0 61 69721.0 62 59495.5 63 45788.0 64 33556.0 65 26252.0 66 19903.5 67 15204.5 68 11814.0 69 9307.0 70 7274.0 71 5204.5 72 3865.0 73 2978.0 74 2155.5 75 1597.5 76 1187.0 77 813.5 78 549.5 79 376.5 80 227.5 81 137.5 82 87.0 83 53.5 84 29.0 85 22.5 86 22.0 87 14.5 88 11.5 89 11.5 90 9.0 91 5.5 92 4.0 93 4.0 94 4.0 95 2.5 96 1.0 97 0.5 98 0.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006327436330565421 2 0.10539431261061209 3 0.11427161134304716 4 0.11625483765561244 5 0.15642304043074418 6 0.09664293300913354 7 0.027041133372913913 8 0.02738741098304436 9 0.022665443572174645 10-11 0.05083984912369728 12-13 0.053263792394610404 14-15 0.027812388050022636 16-17 0.031039065780783606 18-19 0.03634340917232726 20-21 0.05752930295576272 22-23 0.030456689800109675 24-25 0.0485418249837407 26-27 0.05638029088578442 28-29 0.053074913698175616 30-31 0.06735099517037173 32-33 0.049911195532892916 34-35 0.061999432104719386 36-37 0.05338971152556694 38-39 0.032125118285283645 40-41 0.03769703983010991 42-43 0.03340004948621846 44-45 0.007618107422869811 46-47 0.013992763427543927 48-49 0.009506894387217697 50-51 0.008625460470522015 52-53 0.009963351236935104 54-55 0.011820658418543859 56-57 0.014638098973696124 58-59 0.019785043451544113 60-61 0.025089386843087765 62-63 0.012088236571826476 64-65 0.009286535908043778 66-67 0.010844785153630784 68-69 0.01130124200334819 70-71 0.030346510560522715 72-73 0.028536423053022654 74-75 0.029874313819435745 76-77 0.023751496076674677 78-79 0.0052571237174349516 80-81 0.0050052854555219005 82-83 0.006264476765087158 84-85 0.005304343391543649 86-87 0.013457607120978696 88-89 0.038200716353936015 90-91 0.03519439710234896 92-93 0.03022059142956619 94-95 0.047266893782805866 96-97 0.043347660831784 98-99 0.025781942063348655 100-101 0.07534685998611111 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3176642.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.825225071702135 #Duplication Level Percentage of deduplicated Percentage of total 1 72.52781753232121 29.60964474716358 2 13.204402550087515 10.78145412089361 3 5.199163695400019 6.367710841479851 4 2.6218617492106135 4.281523840736398 5 1.528717169012265 3.1205111247950517 6 0.9793303493191865 2.398882915830268 7 0.6577245389646657 1.8796226636889797 8 0.49735134313347096 1.624358441850985 9 0.3691461527589852 1.3563427293664658 >10 2.1059795899835256 15.997844889122733 >50 0.1806941043331536 5.106722298243528 >100 0.11248165035244795 8.631471068945116 >500 0.009135807396499272 2.4774150675272826 >1k 0.00565181305025252 4.910832153674125 >5k 5.419546760516116E-4 1.4556630966821236 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9303 0.29285641882213986 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7393 0.23273003379039878 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6336 0.19945590343513683 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6312 0.1987003886493977 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5910 0.18604551598826685 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5556 0.17490167289861433 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5244 0.1650799806840053 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5000 0.15739891369565723 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4984 0.15689523717183113 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4677 0.14723094387091776 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4561 0.14357928907317855 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4503 0.1417534616743089 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3958 0.12459698008148226 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3869 0.12179527941769956 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3468 0.10917188653930786 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3432 0.10803861436069913 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3356 0.10564615087252514 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3296 0.10375736390817725 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3268 0.10287592999148155 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3262 0.10268705129504677 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.147978273913145E-5 2 0.0 0.0 0.0 0.0 3.147978273913145E-5 3 0.0 0.0 0.0 0.0 3.147978273913145E-5 4 0.0 0.0 0.0 0.0 3.147978273913145E-5 5 0.0 0.0 0.0 0.0 3.147978273913145E-5 6 0.0 0.0 0.0 0.0 3.147978273913145E-5 7 0.0 0.0 0.0 0.0 3.147978273913145E-5 8 0.0 0.0 0.0 0.0 3.147978273913145E-5 9 0.0 0.0 0.0 0.0 3.147978273913145E-5 10-11 0.0 0.0 0.0 0.0 3.147978273913145E-5 12-13 0.0 0.0 0.0 0.0 7.869945684782861E-5 14-15 0.0 0.0 0.0 0.0 9.443934821739434E-5 16-17 0.0 0.0 0.0 3.147978273913145E-5 9.443934821739434E-5 18-19 0.0 0.0 0.0 3.147978273913145E-5 9.443934821739434E-5 20-21 0.0 0.0 0.0 9.443934821739434E-5 1.259191309565258E-4 22-23 0.0 0.0 0.0 2.046185878043544E-4 1.259191309565258E-4 24-25 0.0 0.0 0.0 5.351563065652346E-4 1.4165902232609153E-4 26-27 0.0 0.0 0.0 0.0015582492455870067 1.5739891369565725E-4 28-29 0.0 0.0 0.0 0.004769187084978414 1.5739891369565725E-4 30-31 0.0 0.0 0.0 0.014732538321913517 1.5739891369565725E-4 32-33 0.0 0.0 0.0 0.03387224622730543 1.5739891369565725E-4 34-35 0.0 0.0 0.0 0.06119669764487154 1.5739891369565725E-4 36-37 0.0 0.0 0.0 0.1111708527432427 1.5739891369565725E-4 38-39 0.0 0.0 0.0 0.18961847132915827 1.5739891369565725E-4 40-41 0.0 0.0 0.0 0.2717964441696609 1.5739891369565725E-4 42-43 0.0 0.0 0.0 0.35698073626175064 1.7313880506522296E-4 44-45 0.0 3.147978273913145E-5 0.0 0.46533414844984106 1.8887869643478868E-4 46-47 0.0 3.147978273913145E-5 0.0 0.5944799571371278 1.8887869643478868E-4 48-49 0.0 3.147978273913145E-5 0.0 0.7269626227947625 1.8887869643478868E-4 50-51 0.0 3.147978273913145E-5 0.0 0.8783331580958761 1.8887869643478868E-4 52-53 0.0 3.147978273913145E-5 0.0 1.0220226264086416 2.518382619130516E-4 54-55 0.0 3.147978273913145E-5 0.0 1.1779105105328205 2.518382619130516E-4 56-57 0.0 3.147978273913145E-5 0.0 1.3583526251935218 2.518382619130516E-4 58-59 0.0 3.147978273913145E-5 0.0 1.5551327470958327 2.518382619130516E-4 60-61 0.0 3.147978273913145E-5 0.0 1.7749245901804485 2.518382619130516E-4 62-63 0.0 3.147978273913145E-5 0.0 1.990088905202412 2.83318044652183E-4 64-65 0.0 3.147978273913145E-5 0.0 2.2087632159997885 2.9905793602174875E-4 66-67 0.0 3.147978273913145E-5 0.0 2.4693843372970576 3.147978273913145E-4 68-69 0.0 3.147978273913145E-5 0.0 2.7601001308929365 3.147978273913145E-4 70-71 0.0 3.147978273913145E-5 0.0 3.054499059069294 3.147978273913145E-4 72-73 0.0 3.147978273913145E-5 0.0 3.3705718176615433 3.3053771876088024E-4 74-75 0.0 3.147978273913145E-5 0.0 3.6807106372074663 3.462776101304459E-4 76-77 0.0 3.147978273913145E-5 0.0 4.0131371429326945 3.462776101304459E-4 78-79 0.0 3.147978273913145E-5 0.0 4.3937591960315325 3.462776101304459E-4 80-81 0.0 3.147978273913145E-5 0.0 4.790829435611567 3.462776101304459E-4 82-83 0.0 3.147978273913145E-5 0.0 5.201624860465863 3.462776101304459E-4 84-85 0.0 4.721967410869717E-5 0.0 5.6268222859233115 3.462776101304459E-4 86-87 0.0 6.29595654782629E-5 0.0 6.056914817596695 3.620175015000116E-4 88-89 0.0 6.29595654782629E-5 0.0 6.507170150114492 3.7775739286957736E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5800 0.0 50.506165 1 GTATCAA 10580 0.0 44.992493 1 TATCAAC 13960 0.0 33.89504 2 ATCAACG 14130 0.0 33.352623 3 AGGTAAG 1985 0.0 33.300655 2 TCAACGC 14430 0.0 32.65922 4 TAAGGTG 2240 0.0 32.481934 5 CAACGCA 14655 0.0 32.157803 5 AAGGTAA 2035 0.0 31.547215 1 AACGCAG 15180 0.0 31.14834 6 GTAAGGT 2475 0.0 28.629225 4 ACGCAGA 17175 0.0 27.286379 7 CGCAGAG 17475 0.0 26.790743 8 TGGTATC 2705 0.0 26.72236 2 GTGTAAG 965 0.0 26.610811 1 TAAGGTA 1010 0.0 26.367268 4 GGTAAGG 2730 0.0 25.258286 3 TACATGG 13790 0.0 25.243254 2 GTACATG 14125 0.0 25.11551 1 ACATGGG 14010 0.0 23.794598 3 >>END_MODULE