FastQCFastQC Report
Thu 26 May 2016
SRR938375_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938375_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3176642
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90170.2838532009587483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76380.24044258056148599No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA64190.20206872540248477No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA62630.19715787929518025No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA60100.18919349426218No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC58770.18500668315787552No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA56440.1776718937796579No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG53300.16778724199957062No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52440.1650799806840053No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA46610.14672726734709168No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA43110.1357093433883957No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA42580.1340409149032217No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA39530.12443958116778661No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG39290.12368406638204746No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT36280.11420865177756889No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT36090.11361053590552539No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG35470.11165878937569926No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT34180.10759789740235129No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA33430.10523691369691643No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG32740.10306480868791637No Hit
CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT31930.10051494628604671No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55900.053.3013081
GTATCAA101000.044.227111
TATCAAC135100.032.817722
ATCAACG135250.032.730723
AGGTAAG18450.032.195542
TCAACGC138450.032.0085224
CAACGCA141350.031.3182165
AACGCAG147500.030.0635536
TAAGGTG23600.029.1831995
AAGGTAA20350.028.7225381
GTAAGGT24300.027.3652024
ACGCAGA168800.026.1574847
CGCAGAG171750.025.6805578
GGTAAGG27000.024.2768463
TACATGG131550.023.6248472
GTACATG134700.023.5309981
ACATGGG132700.022.7289683
TGGTATC25600.022.4609182
GCAGAGT194400.022.0046279
AGAGTAC166600.021.21656810-11
Produced by FastQC (version 0.11.5)