##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938375_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3176642 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.84297317733632 31.0 31.0 34.0 30.0 34.0 2 32.063101854096246 33.0 31.0 34.0 30.0 34.0 3 32.118841846201114 33.0 31.0 34.0 30.0 34.0 4 35.788897521344865 37.0 35.0 37.0 35.0 37.0 5 35.68259155422613 37.0 35.0 37.0 33.0 37.0 6 35.686636391510284 37.0 35.0 37.0 33.0 37.0 7 35.62537799349124 37.0 35.0 37.0 33.0 37.0 8 35.59476547876657 37.0 35.0 37.0 33.0 37.0 9 37.189942083495716 39.0 37.0 39.0 33.0 39.0 10-11 37.08836847211616 39.0 37.0 39.0 33.0 39.0 12-13 36.98267305538364 39.0 37.0 39.0 33.0 39.0 14-15 38.04732591837544 40.0 37.0 41.0 33.0 41.0 16-17 37.952423502553955 40.0 37.0 41.0 33.0 41.0 18-19 38.182388981824204 40.0 37.5 41.0 33.0 41.0 20-21 38.140349463364146 40.0 37.0 41.0 33.0 41.0 22-23 38.04058436550294 40.0 37.0 41.0 33.0 41.0 24-25 37.9017393209559 40.0 37.0 41.0 32.5 41.0 26-27 37.703174767569024 40.0 37.0 41.0 32.0 41.0 28-29 37.59249122186258 40.0 37.0 41.0 32.0 41.0 30-31 37.330575809298 39.0 36.0 41.0 31.0 41.0 32-33 37.123848863044685 39.0 36.0 41.0 31.0 41.0 34-35 37.036880769063686 39.0 36.0 41.0 31.0 41.0 36-37 36.97988284484056 39.0 36.0 41.0 31.0 41.0 38-39 36.808218552798834 39.0 35.0 41.0 30.0 41.0 40-41 36.62327010723903 39.0 35.0 41.0 30.0 41.0 42-43 36.38858628073292 39.0 35.0 40.0 30.0 41.0 44-45 36.08001987633482 38.5 35.0 40.0 29.5 41.0 46-47 35.681278375089164 38.0 35.0 40.0 28.5 41.0 48-49 35.294268129679075 38.0 34.0 40.0 27.0 41.0 50-51 34.890001454365965 38.0 34.0 40.0 26.0 41.0 52-53 34.375484867353634 37.5 33.0 40.0 24.5 41.0 54-55 34.011557015238104 37.0 33.0 40.0 24.0 41.0 56-57 33.639343054709975 37.0 32.5 39.5 23.0 41.0 58-59 33.134751728397475 36.0 32.0 39.0 22.0 40.5 60-61 32.71129214434613 35.5 31.0 39.0 21.5 40.0 62-63 32.20215324861914 35.0 31.0 38.5 19.5 40.0 64-65 31.654551567346903 35.0 30.5 38.0 18.0 40.0 66-67 31.140510167655027 34.5 30.0 37.5 16.0 39.5 68-69 30.6345612757119 34.0 29.0 37.0 12.5 39.0 70-71 30.046644223680225 34.0 29.0 36.0 8.0 39.0 72-73 29.562222938562165 34.0 28.5 36.0 7.0 38.5 74-75 28.99482220533507 33.5 27.5 35.0 6.0 37.5 76-77 27.798215222237822 31.5 26.0 34.0 2.0 36.0 78-79 28.490721648835468 33.0 27.0 35.0 2.0 36.0 80-81 28.16175209545174 33.0 27.0 35.0 2.0 36.0 82-83 27.74586371394699 33.0 26.0 35.0 2.0 36.0 84-85 27.228966310966108 32.5 25.5 35.0 2.0 35.0 86-87 26.661758863605026 32.0 25.0 34.5 2.0 35.0 88-89 25.92292143716541 32.0 23.5 34.0 2.0 35.0 90-91 25.34108958453612 31.0 21.0 34.0 2.0 35.0 92-93 24.54572186604597 31.0 18.5 34.0 2.0 35.0 94-95 23.759957527477127 31.0 11.0 34.0 2.0 35.0 96-97 22.71416892429175 30.0 2.0 34.0 2.0 35.0 98-99 21.377738504999932 29.5 2.0 34.0 2.0 35.0 100-101 17.42438713584974 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.295046453507787 1101 2 0.31290266916909104 1101 3 0.31681837324199336 1101 4 0.21762000657935943 1101 5 0.12576441786179515 1101 6 0.20264407379607974 1101 7 0.18953053946052734 1101 8 0.2622187942340233 1101 9 0.2741928326807326 1101 10-11 0.27121870778059787 1101 12-13 0.36837241276098354 1101 14-15 0.413398828123988 1101 16-17 0.48776113091069107 1101 18-19 0.39034044113043365 1101 20-21 0.25789911265647447 1101 22-23 0.30823051505556265 1101 24-25 0.36664758751027193 1101 26-27 0.34032300143683614 1101 28-29 0.31684553392928194 1101 30-31 0.5455758666197355 1101 32-33 0.48239382723298263 1101 34-35 0.4908563755897717 1101 36-37 0.42073587123927325 1101 38-39 0.5316233040262048 1101 40-41 0.5844001030010091 1101 42-43 0.6680770637281555 1101 44-45 0.6143479799942284 1101 46-47 0.5769318245182191 1101 48-49 0.5826227084826385 1101 50-51 0.4610610552425598 1101 52-53 0.5839062640687018 1101 54-55 0.6645557361332806 1101 56-57 0.5269084213288906 1101 58-59 0.7134228155859219 1101 60-61 0.5697245032023872 1101 62-63 0.5866675122900631 1101 64-65 0.45380652192300985 1101 66-67 0.35954871483376394 1101 68-69 0.45936677021324 1101 70-71 0.39876733232291883 1101 72-73 0.43905478201021 1101 74-75 0.6132354048201343 1101 76-77 0.5544832917118683 1101 78-79 0.7840621223525126 1101 80-81 0.7907499407931304 1101 82-83 0.631603677609089 1101 84-85 0.8719055523225414 1101 86-87 0.904234692734228 1101 88-89 0.8200909114266537 1101 90-91 0.9095983703535744 1101 92-93 1.2420332367908777 1101 94-95 1.1145283994176403 1101 96-97 1.2047036098367485 1101 98-99 1.3141048723392892 1101 100-101 1.592516162560468 1102 1 0.22104783572738995 1102 2 0.2107450082757012 1102 3 0.19250770422667074 1102 4 0.11663135451571094 1102 5 0.06192864089536698 1102 6 0.04943808533272431 1102 7 0.13470913596933798 1102 8 0.18745011802793954 1102 9 0.1607619767625863 1102 10-11 0.20515578770865517 1102 12-13 0.21912777659596117 1102 14-15 0.3001701697741552 1102 16-17 0.33792394697587014 1102 18-19 0.21716354486309797 1102 20-21 0.3395022894104329 1102 22-23 0.3395723755825557 1102 24-25 0.337359999829971 1102 26-27 0.25423317943627666 1102 28-29 0.1269950722716473 1102 30-31 0.24243178105329122 1102 32-33 0.2201977191030693 1102 34-35 0.256949551050603 1102 36-37 0.354511341605388 1102 38-39 0.16333441641599933 1102 40-41 0.09413243871085086 1102 42-43 0.03354347622481413 1102 44-45 0.28027781347822156 1102 46-47 0.29748779358470756 1102 48-49 0.4006265210328692 1102 50-51 0.40080568491103463 1102 52-53 0.17598399257578023 1102 54-55 0.22037012272538448 1102 56-57 0.39897007550051455 1102 58-59 0.3690741076890234 1102 60-61 0.21543130064163307 1102 62-63 0.4433792279314872 1102 64-65 0.468611244733534 1102 66-67 0.4583628149586829 1102 68-69 0.5266548169834948 1102 70-71 0.4890531250976373 1102 72-73 0.5620014217749443 1102 74-75 0.6810945297897391 1102 76-77 0.6581902883788224 1102 78-79 0.7409549990955249 1102 80-81 0.643543367021028 1102 82-83 0.7018144939415549 1102 84-85 0.631942762119845 1102 86-87 0.5546732721292571 1102 88-89 0.5580964440173979 1102 90-91 0.5844117181154509 1102 92-93 0.8653788888316285 1102 94-95 0.8623142184879384 1102 96-97 0.8337831839844156 1102 98-99 0.6039688220844077 1102 100-101 0.785868020358901 1103 1 0.2688370982923445 1103 2 0.1925449335983771 1103 3 0.17526080977773972 1103 4 0.1429380961976463 1103 5 0.09410912251481562 1103 6 0.15643963590807175 1103 7 0.21058899419038113 1103 8 0.1659744030606234 1103 9 0.2670688545628366 1103 10-11 0.20292010307225183 1103 12-13 0.2803706916963833 1103 14-15 0.4213771918832876 1103 16-17 0.4462404474021824 1103 18-19 0.4289015081904779 1103 20-21 0.32932944027341193 1103 22-23 0.3389023449160433 1103 24-25 0.4073722786406435 1103 26-27 0.27875806198142783 1103 28-29 0.40310467708497555 1103 30-31 0.21524824967954714 1103 32-33 0.37627790123875826 1103 34-35 0.4346328830579651 1103 36-37 0.4413609864891299 1103 38-39 0.5260667240274302 1103 40-41 0.5835057640742249 1103 42-43 0.6274292058389221 1103 44-45 0.5637701940209041 1103 46-47 0.5659692576732951 1103 48-49 0.70075842671978 1103 50-51 0.7553292954540112 1103 52-53 0.7071590761653255 1103 54-55 0.6782166864068131 1103 56-57 0.7805011436842904 1103 58-59 0.8513538500686835 1103 60-61 0.7785695459216697 1103 62-63 0.7929125181293912 1103 64-65 0.8720559533570231 1103 66-67 0.8532391690718839 1103 68-69 0.753856290159149 1103 70-71 0.5780285038761797 1103 72-73 0.494964794918193 1103 74-75 0.6236724322957024 1103 76-77 0.5535305595218141 1103 78-79 0.8495855103911119 1103 80-81 0.9145928321816612 1103 82-83 0.9900092341890883 1103 84-85 0.9568339437418025 1103 86-87 0.9590782602552785 1103 88-89 0.8488695702255953 1103 90-91 0.8485774102453938 1103 92-93 1.196867584322991 1103 94-95 1.2753956930435528 1103 96-97 1.2646519778987049 1103 98-99 1.4293854673359085 1103 100-101 1.615523108285469 1104 1 0.24287751443194594 1104 2 0.16175084863910172 1104 3 0.20214354926839917 1104 4 0.1509566944490075 1104 5 0.032108077383298905 1104 6 0.007883975195845494 1104 7 0.06545480097092593 1104 8 0.1499404972742724 1104 9 0.2319781909394294 1104 10-11 0.2573493195439127 1104 12-13 0.180788130036035 1104 14-15 0.25587697447662805 1104 16-17 0.3258847999405745 1104 18-19 0.2896813904179396 1104 20-21 0.27359446754906713 1104 22-23 0.21576648089019557 1104 24-25 0.34947837660131853 1104 26-27 0.30414791314890266 1104 28-29 0.2989009624365977 1104 30-31 0.3189941159913374 1104 32-33 0.35459021566978066 1104 34-35 0.38053284791310915 1104 36-37 0.33094615439684105 1104 38-39 0.26262436706719683 1104 40-41 0.2778397867029412 1104 42-43 0.3835502673450435 1104 44-45 0.41723207151061104 1104 46-47 0.26641841851868264 1104 48-49 0.3153526879737001 1104 50-51 0.3844617222169191 1104 52-53 0.48828497502449864 1104 54-55 0.3970109328305682 1104 56-57 0.46585129126194147 1104 58-59 0.6722221692014827 1104 60-61 0.5615143334050146 1104 62-63 0.46056453496557026 1104 64-65 0.46011102590854236 1104 66-67 0.3214425125274616 1104 68-69 0.3490481586673866 1104 70-71 0.5229408150603589 1104 72-73 0.2837428159164723 1104 74-75 0.44161322046175755 1104 76-77 0.48353022167493265 1104 78-79 0.7809131959776465 1104 80-81 0.7489717357939476 1104 82-83 0.7948641596675259 1104 84-85 0.8702205340287499 1104 86-87 0.9298185266453487 1104 88-89 0.8829760902754913 1104 90-91 1.0392092672756128 1104 92-93 1.2532310838726026 1104 94-95 1.2873666610275158 1104 96-97 1.3228419347201203 1104 98-99 1.4038797829524654 1104 100-101 1.811768598358693 1105 1 0.2367615769222553 1105 2 0.2481505730444482 1105 3 0.2816755930011752 1105 4 0.19596216792805166 1105 5 0.11456646548737126 1105 6 0.1733509752219362 1105 7 0.23854286821141812 1105 8 0.2523029777822572 1105 9 0.328518898771506 1105 10-11 0.24606978263167179 1105 12-13 0.3077614429996842 1105 14-15 0.4310719562546339 1105 16-17 0.29844802329693465 1105 18-19 0.33138351812121414 1105 20-21 0.3463971312198595 1105 22-23 0.3716864249167031 1105 24-25 0.3164499516917232 1105 26-27 0.22348984669518046 1105 28-29 0.38595238931064557 1105 30-31 0.49917639342287856 1105 32-33 0.5341840607225592 1105 34-35 0.5522870049942554 1105 36-37 0.48391094356466624 1105 38-39 0.35547553506797414 1105 40-41 0.46806480431277464 1105 42-43 0.6012658309038557 1105 44-45 0.5155305785624478 1105 46-47 0.5097107618723129 1105 48-49 0.5796313401317903 1105 50-51 0.7040056681218161 1105 52-53 0.7134096099119205 1105 54-55 0.8035347672691842 1105 56-57 0.676493597711449 1105 58-59 0.7922731591421481 1105 60-61 0.7619304574998509 1105 62-63 0.8399872015574701 1105 64-65 0.7331543829702092 1105 66-67 0.7249205494866438 1105 68-69 0.7775817675132046 1105 70-71 0.8523424669438064 1105 72-73 0.8800174504238569 1105 74-75 0.8085285406361464 1105 76-77 0.843095067251749 1105 78-79 1.139492579090163 1105 80-81 1.0301196729733206 1105 82-83 1.0393450768537562 1105 84-85 1.1149754333009163 1105 86-87 1.0608232022639115 1105 88-89 1.0404914984939069 1105 90-91 1.194759348325011 1105 92-93 1.4870642406454095 1105 94-95 1.661113404826171 1105 96-97 1.8468534474114229 1105 98-99 1.6895680680639238 1105 100-101 1.5834132345032934 1106 1 -0.01667898782068633 1106 2 0.023114464786580413 1106 3 0.056335845400248274 1106 4 0.0027195913751683065 1106 5 -0.06468912467035182 1106 6 -0.058031992497944884 1106 7 0.05247269896553064 1106 8 0.07793427566234357 1106 9 -0.1962352759667283 1106 10-11 0.08513472482061957 1106 12-13 0.052619428285545666 1106 14-15 0.06364166035410079 1106 16-17 0.0606011400705313 1106 18-19 0.004066613200095048 1106 20-21 0.12019121934469013 1106 22-23 0.14756007421861028 1106 24-25 0.14561281025279982 1106 26-27 0.1221954490277497 1106 28-29 0.0642808653226794 1106 30-31 0.08225651737715367 1106 32-33 0.08557454021136834 1106 34-35 0.08900577068266813 1106 36-37 0.11693726714814545 1106 38-39 0.08944180859787565 1106 40-41 0.0032418611665931962 1106 42-43 -0.06825458139982032 1106 44-45 -0.139663738003307 1106 46-47 -0.8946634812367407 1106 48-49 -0.5113570296438112 1106 50-51 -0.06334115470972534 1106 52-53 -0.13622690584226405 1106 54-55 -0.06949282576691473 1106 56-57 -0.06385883987472596 1106 58-59 -0.3498323238941552 1106 60-61 -0.26825602589742203 1106 62-63 -0.44223244946819307 1106 64-65 -0.32634464945057573 1106 66-67 -0.20870751280291344 1106 68-69 -0.37463286725730427 1106 70-71 -0.25417111665333536 1106 72-73 0.047855838005098406 1106 74-75 0.03189180701644645 1106 76-77 -0.07205852390156053 1106 78-79 0.15523631434652074 1106 80-81 -0.36944296124379505 1106 82-83 0.05929223412248774 1106 84-85 0.2681167785755818 1106 86-87 0.4829274501571277 1106 88-89 0.5078431549328037 1106 90-91 0.46215264038801607 1106 92-93 0.5067897482574324 1106 94-95 0.6713927923768388 1106 96-97 0.7480553708036908 1106 98-99 0.86699041928998 1106 100-101 0.9757162748423163 1107 1 0.13570283932867966 1107 2 0.13388204705275797 1107 3 0.18904908370799944 1107 4 0.052297251899673824 1107 5 -0.008767592168716476 1107 6 0.1006431501600602 1107 7 0.1107612197141421 1107 8 0.1238941577538526 1107 9 0.18896777193224068 1107 10-11 0.19495027282571442 1107 12-13 0.18574917856745543 1107 14-15 0.2607781188233176 1107 16-17 0.2316450095777256 1107 18-19 0.13324220121813113 1107 20-21 0.2524841346467781 1107 22-23 0.26876341850970675 1107 24-25 0.2031002425322157 1107 26-27 0.21480583000739983 1107 28-29 0.2331227304781649 1107 30-31 0.16193722626375262 1107 32-33 0.20383750485812158 1107 34-35 0.3502855555822606 1107 36-37 0.3372977656426457 1107 38-39 0.2755576805503068 1107 40-41 0.29933252435167645 1107 42-43 0.31536447638197984 1107 44-45 0.2364124683473534 1107 46-47 0.2633384595913526 1107 48-49 0.25608996903863357 1107 50-51 0.3879110248143647 1107 52-53 0.38413123849606734 1107 54-55 0.19966199743595325 1107 56-57 0.2176477293787613 1107 58-59 0.06980604684452629 1107 60-61 0.04712574104729583 1107 62-63 0.3659384117130102 1107 64-65 0.18175012617518504 1107 66-67 0.2452296590142069 1107 68-69 0.34246331391546647 1107 70-71 0.5371983056367426 1107 72-73 0.5913479565989945 1107 74-75 0.31325239929126525 1107 76-77 0.23606004413857207 1107 78-79 0.5605486799956019 1107 80-81 0.5865259245302319 1107 82-83 0.6995054376967893 1107 84-85 0.7275368186965814 1107 86-87 0.8299880063863441 1107 88-89 0.8078202019101788 1107 90-91 0.949101883623289 1107 92-93 1.2606452578704932 1107 94-95 1.1937290550265693 1107 96-97 1.1398768779113126 1107 98-99 1.4983230511362322 1107 100-101 1.5544136411204583 1108 1 0.20068084449795975 1108 2 0.18787394880550323 1108 3 0.1578058662751758 1108 4 0.17749111836209863 1108 5 0.1668146325942459 1108 6 0.17155523484623103 1108 7 0.19728268150404915 1108 8 0.16083160473486657 1108 9 0.1714004171316006 1108 10-11 0.21389708683089736 1108 12-13 0.2742710148470522 1108 14-15 0.431306848214426 1108 16-17 0.3185799090724899 1108 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64-65 -0.8700405545643903 2316 66-67 -1.008439228151829 2316 68-69 -0.9765483843408553 2316 70-71 -1.0004363691121263 2316 72-73 -1.0042982854724158 2316 74-75 -1.0334992012613675 2316 76-77 -1.0370521067745493 2316 78-79 -1.1684809523191113 2316 80-81 -1.1340125229175904 2316 82-83 -1.0714176035531544 2316 84-85 -1.1437560782424079 2316 86-87 -1.3013409166929613 2316 88-89 -1.3755178130046595 2316 90-91 -1.3890190521082175 2316 92-93 -1.6896255428637694 2316 94-95 -1.6425884774585562 2316 96-97 -1.8561228692510383 2316 98-99 -1.587887251095058 2316 100-101 -1.71259235151887 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 13.0 4 44.0 5 111.0 6 228.0 7 677.0 8 1528.0 9 2592.0 10 4305.0 11 6771.0 12 9243.0 13 12923.0 14 16992.0 15 21957.0 16 27914.0 17 35251.0 18 33659.0 19 29791.0 20 29708.0 21 31398.0 22 33908.0 23 37515.0 24 43424.0 25 50820.0 26 59792.0 27 71977.0 28 85771.0 29 102606.0 30 120702.0 31 144884.0 32 173913.0 33 214812.0 34 275026.0 35 362059.0 36 462977.0 37 473259.0 38 191360.0 39 6727.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.42406890320875 18.146400430645055 14.837296128286894 23.5922345378593 2 16.790461513361684 19.86818313385371 37.53583845851243 25.80551689427218 3 19.83566294218864 22.86087006341917 29.177603267853286 28.125863726538903 4 14.082174876599174 16.136263473355495 34.33656316107586 35.444998488969475 5 14.712696241090258 36.75371066044843 33.03290904189248 15.500684056568833 6 34.69541106615099 34.456542474726454 15.973250999010904 14.874795460111653 7 30.761697415069122 31.86679518812633 18.854280715296216 18.517226681508333 8 28.49087180739913 33.18507404989294 18.611067913853685 19.71298622885424 9 27.291964281779315 15.960470207218819 18.68696567003773 38.06059984096414 10-11 26.32090742362532 25.64086541700324 25.824707348199766 22.213519811171672 12-13 27.206717030121748 22.680144630713816 26.29734480624509 23.81579353291935 14-15 23.476047977707275 24.179322063990842 24.396532564890848 27.948097393411032 16-17 23.52383428790528 27.430443846048753 25.518220183451586 23.527501682594387 18-19 23.78538406279335 25.57439585574956 27.615812546708128 23.02440753474896 20-21 23.905967370575596 24.704782597472423 28.055884169509817 23.33336586244216 22-23 23.91382157636901 24.52196375921492 28.19352951953667 23.370685144879406 24-25 23.862185513640394 24.95405611867035 27.92929923362064 23.254459134068615 26-27 22.92294468179681 25.4163696373585 27.795474052479786 23.865211628364904 28-29 23.374288556462176 25.839692253450185 27.603820388838525 23.18219880124912 30-31 23.163222695283732 25.278903679559477 27.429921303964523 24.12795232119227 32-33 22.793977144983383 25.226962006627772 27.5491377190881 24.42992312930074 34-35 24.04977708206547 25.34857589740958 27.21950608274268 23.382140937782268 36-37 24.167216906646868 24.63488153073456 27.299869296357205 23.898032266261364 38-39 23.3784703328129 25.07309505434337 27.30920951250183 24.239225100341898 40-41 23.84473046491583 24.92540809124673 27.611850087105218 23.61801135673222 42-43 23.55347646821334 24.996619821617873 27.219998063972334 24.229905646196453 44-45 23.427332365244837 25.26490600490341 27.587059096686854 23.720702533164904 46-47 24.226683397121867 25.68788047252413 26.510573114628595 23.574863015725413 48-49 23.686238487056457 25.83602433009448 26.39102863967674 24.08670854317232 50-51 23.963781011332483 25.849637082925536 26.602638463952733 23.58394344178925 52-53 24.553679640324592 25.23726312250483 26.585290378959918 23.62376685821065 54-55 23.6464416245093 25.820124724940896 26.69710286816996 23.836330782379846 56-57 23.656946549217697 26.050197031960167 26.444922027726133 23.847934391096008 58-59 23.62082618970026 25.32553713036127 26.434260470637504 24.619376209300974 60-61 22.971269661485305 25.860452641500046 26.933692874425258 24.23458482258939 62-63 23.37844100385162 25.83439168397702 27.07146558634095 23.71570172583041 64-65 23.00474472318775 25.943655231474484 26.70235441539469 24.349245629943077 66-67 22.834216821043523 25.986470328602017 26.783860225297236 24.395452625057217 68-69 23.739029408662496 25.264230873979294 27.036936751146452 23.95980296621176 70-71 23.465214272751357 25.54314849288108 26.38269895507534 24.60893827929223 72-73 23.26598486607301 25.78342665790478 26.36152220705069 24.589066268971514 74-75 23.228976564989427 26.26071653927099 26.217579595532904 24.29272730020668 76-77 23.42322849370293 25.6721901364923 26.564858448782907 24.33972292102186 78-79 23.43802154118609 25.944977522952534 26.620737453726505 23.99626348213487 80-81 23.7164123671542 25.936912896860665 26.238520468207298 24.108154267777834 82-83 23.555668923796862 26.00909937592358 26.284127580209997 24.151104120069565 84-85 23.237618257198704 26.182571987023195 26.345112665484514 24.23469709029359 86-87 23.7156352147895 26.18365822578576 25.860657382825813 24.240049176598927 88-89 23.322968465734423 26.364155778934844 25.59773108170119 24.715144673629545 90-91 23.10030533913678 26.290181443302863 25.926416322371626 24.683096895188726 92-93 23.20931183038118 25.756043848087174 26.178762129731847 24.8558821917998 94-95 22.97277893654968 25.40297237588545 26.535357027699686 25.08889165986518 96-97 23.31055334554368 25.022327558190216 26.43138857887139 25.23573051739471 98-99 22.82402001274025 25.610811415199002 25.96345958357185 25.601708988488898 100-101 22.97079607470077 24.938514696416167 26.577125782645773 25.5135634462373 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 428.0 1 317.5 2 314.0 3 417.5 4 375.5 5 387.0 6 663.0 7 1461.0 8 2278.5 9 2562.5 10 2242.0 11 1854.0 12 1886.0 13 2072.5 14 2272.0 15 2424.5 16 2671.5 17 2997.5 18 3336.5 19 3673.5 20 4109.5 21 4814.0 22 5830.0 23 7003.0 24 8166.5 25 9879.0 26 12384.0 27 15593.0 28 18623.5 29 21324.0 30 24652.0 31 31881.0 32 44641.5 33 46846.0 34 41295.0 35 42489.5 36 46820.0 37 52474.5 38 59544.0 39 64753.0 40 69255.0 41 76741.0 42 81930.5 43 87088.0 44 93324.5 45 101777.0 46 107579.5 47 112440.5 48 115924.5 49 114937.0 50 116619.5 51 118473.0 52 121998.5 53 130841.5 54 139762.5 55 135077.0 56 127046.0 57 120804.0 58 115187.5 59 105147.0 60 84043.0 61 68712.0 62 59148.5 63 44531.0 64 31168.5 65 23950.0 66 18229.5 67 14158.5 68 11002.0 69 8618.0 70 6765.0 71 4539.5 72 3247.0 73 2685.5 74 1994.5 75 1431.5 76 1047.0 77 705.5 78 468.0 79 275.5 80 160.0 81 99.5 82 68.0 83 46.5 84 21.0 85 8.5 86 7.0 87 5.0 88 1.5 89 3.0 90 3.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.7775739286957736E-4 2 0.05134352564752338 3 0.0 4 6.29595654782629E-5 5 0.0461493614955667 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006689453832065433 26-27 0.0014952896801087438 28-29 6.29595654782629E-5 30-31 0.001652688593804401 32-33 2.83318044652183E-4 34-35 0.011364201568826452 36-37 0.023767235968044243 38-39 0.0040136722992392595 40-41 7.555147857391547E-4 42-43 0.001086052504500035 44-45 0.00879859927558724 46-47 0.0 48-49 0.0 50-51 6.610754375217604E-4 52-53 0.0 54-55 0.0016369487024348352 56-57 0.0 58-59 0.023200599878739875 60-61 0.0 62-63 0.009947611345565537 64-65 0.015173255280261358 66-67 0.006799633071652393 68-69 0.016904643330913585 70-71 4.721967410869717E-5 72-73 1.416590223260915E-4 74-75 0.022350645744783328 76-77 0.04893532226797983 78-79 0.030157631864087927 80-81 0.026018040433892144 82-83 0.0361860102586316 84-85 0.016180608327913563 86-87 0.01052998732623947 88-89 0.08242981110241569 90-91 0.02609673989073997 92-93 0.02417647314365295 94-95 0.027261491852087837 96-97 0.03758686059052295 98-99 0.052398098369284295 100-101 0.015000116475196135 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3176642.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.4346553014657 #Duplication Level Percentage of deduplicated Percentage of total 1 68.80115205560685 25.75547411545371 2 14.938234193095449 11.184152956621936 3 5.870667504773186 6.592992432920999 4 3.0206942911194252 4.523145982366438 5 1.7175018124205834 3.2147044163803575 6 1.0973990921057613 2.4648454044672357 7 0.7745270161193702 2.029590630907098 8 0.5605018742386608 1.6785755566359795 9 0.4396809457492383 1.4813374182070662 >10 2.414641233124852 16.81007551860942 >50 0.21626291544145415 5.608069112300696 >100 0.1307294427517446 9.23267515948586 >500 0.010770914962888775 2.728535644096188 >1k 0.0064793785322954465 4.886378019192641 >5k 7.57329958320247E-4 1.8094476323545081 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9017 0.2838532009587483 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7638 0.24044258056148599 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6419 0.20206872540248477 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6263 0.19715787929518025 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6010 0.18919349426218 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5877 0.18500668315787552 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5644 0.1776718937796579 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5330 0.16778724199957062 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5244 0.1650799806840053 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4661 0.14672726734709168 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4311 0.1357093433883957 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4258 0.1340409149032217 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3953 0.12443958116778661 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3929 0.12368406638204746 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3628 0.11420865177756889 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3609 0.11361053590552539 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3547 0.11165878937569926 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3418 0.10759789740235129 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3343 0.10523691369691643 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3274 0.10306480868791637 No Hit CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT 3193 0.10051494628604671 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 6.29595654782629E-5 14-15 0.0 0.0 0.0 0.0 6.29595654782629E-5 16-17 0.0 0.0 0.0 3.147978273913145E-5 6.29595654782629E-5 18-19 0.0 0.0 0.0 3.147978273913145E-5 6.29595654782629E-5 20-21 0.0 0.0 0.0 9.443934821739434E-5 6.29595654782629E-5 22-23 0.0 0.0 0.0 2.046185878043544E-4 6.29595654782629E-5 24-25 0.0 0.0 0.0 5.508961979348003E-4 6.29595654782629E-5 26-27 0.0 0.0 0.0 0.001542509354217441 6.29595654782629E-5 28-29 0.0 0.0 0.0 0.004769187084978414 6.29595654782629E-5 30-31 0.0 0.0 0.0 0.014779757996022214 6.29595654782629E-5 32-33 0.0 0.0 0.0 0.034124084489218484 6.29595654782629E-5 34-35 0.0 0.0 0.0 0.06168463427732808 6.29595654782629E-5 36-37 0.0 0.0 0.0 0.11222542546500361 6.29595654782629E-5 38-39 0.0 0.0 0.0 0.19117672057474527 6.29595654782629E-5 40-41 0.0 0.0 0.0 0.2736537513512697 6.29595654782629E-5 42-43 0.0 0.0 0.0 0.35912136148801155 6.29595654782629E-5 44-45 0.0 0.0 0.0 0.4675534731329498 6.29595654782629E-5 46-47 0.0 0.0 0.0 0.5966363222547584 6.29595654782629E-5 48-49 0.0 0.0 0.0 0.7295597048707408 6.29595654782629E-5 50-51 0.0 0.0 0.0 0.8807571013667892 6.29595654782629E-5 52-53 0.0 0.0 0.0 1.0242104713090112 9.443934821739434E-5 54-55 0.0 0.0 0.0 1.18009835543319 1.259191309565258E-4 56-57 0.0 0.0 0.0 1.360666389224848 1.259191309565258E-4 58-59 0.0 0.0 0.0 1.5579344477596153 1.259191309565258E-4 60-61 0.0 0.0 0.0 1.7784031061731225 1.259191309565258E-4 62-63 0.0 0.0 0.0 1.9939766583706946 1.259191309565258E-4 64-65 0.0 0.0 0.0 2.212902807429984 1.5739891369565725E-4 66-67 0.0 0.0 0.0 2.475318276343384 1.7313880506522296E-4 68-69 0.0 0.0 0.0 2.7670886426610237 1.8887869643478868E-4 70-71 0.0 0.0 0.0 3.064100392804729 1.8887869643478868E-4 72-73 0.0 0.0 0.0 3.3846590204373044 2.046185878043544E-4 74-75 0.0 0.0 0.0 3.6992207494580756 2.3609837054348586E-4 76-77 0.0 0.0 0.0 4.037911731948391 2.518382619130516E-4 78-79 0.0 0.0 0.0 4.426183372252838 2.6757815328261726E-4 80-81 0.0 0.0 0.0 4.833704899702264 2.9905793602174875E-4 82-83 0.0 0.0 0.0 5.257643133850148 3.147978273913145E-4 84-85 0.0 0.0 0.0 5.69650278501638 3.147978273913145E-4 86-87 0.0 0.0 0.0 6.140414941312241 3.147978273913145E-4 88-89 0.0 0.0 0.0 6.609117426515169 3.147978273913145E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5590 0.0 53.301308 1 GTATCAA 10100 0.0 44.22711 1 TATCAAC 13510 0.0 32.81772 2 ATCAACG 13525 0.0 32.73072 3 AGGTAAG 1845 0.0 32.19554 2 TCAACGC 13845 0.0 32.008522 4 CAACGCA 14135 0.0 31.318216 5 AACGCAG 14750 0.0 30.063553 6 TAAGGTG 2360 0.0 29.183199 5 AAGGTAA 2035 0.0 28.722538 1 GTAAGGT 2430 0.0 27.365202 4 ACGCAGA 16880 0.0 26.157484 7 CGCAGAG 17175 0.0 25.680557 8 GGTAAGG 2700 0.0 24.276846 3 TACATGG 13155 0.0 23.624847 2 GTACATG 13470 0.0 23.530998 1 ACATGGG 13270 0.0 22.728968 3 TGGTATC 2560 0.0 22.460918 2 GCAGAGT 19440 0.0 22.004627 9 AGAGTAC 16660 0.0 21.216568 10-11 >>END_MODULE