FastQCFastQC Report
Thu 26 May 2016
SRR938374_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938374_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3175555
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95410.3004514171538518No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73170.2304164153982532No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA64310.2025157807060498No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA63660.2004688944137324No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA59450.1872113693511843No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA55170.17373341038023274No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC53320.16790765708671399No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50100.15776769730015697No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA49190.1549020564909126No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG47110.1483520203554969No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA46650.14690345467170304No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA44310.13953466401936038No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT40130.1263716106318423No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG38250.12045138566329351No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA35000.11021695420170648No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA34690.10924074689306279No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT33130.104328219791501No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG32860.1034779747162307No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT32840.10341499359954402No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG32700.1029741257827372No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58800.051.4928251
GTATCAA107750.043.8289761
TATCAAC138300.033.7090452
AGGTAAG19850.033.517162
ATCAACG140700.033.1340523
TCAACGC144350.032.296234
CAACGCA146350.031.8533695
TAAGGTG21950.031.8245225
AACGCAG151800.030.9235216
AAGGTAA21500.029.6710741
GTAAGGT25250.027.4783764
ACGCAGA171150.027.3414867
CGCAGAG173300.026.838228
TAAGGTA10550.026.5765844
GTGTAAG10100.025.9243721
GGTAAGG26800.025.8891433
TGTAAGG12050.024.0570532
TACATGG131500.023.7793392
GTACATG134350.023.6704061
GCAGAGT192750.023.5370049
Produced by FastQC (version 0.11.5)