##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938374_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3175555 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.07885834129782 31.0 31.0 34.0 28.0 34.0 2 31.166452163480084 31.0 31.0 34.0 27.0 34.0 3 31.233408018440873 31.0 31.0 34.0 27.0 34.0 4 34.87638097907295 37.0 35.0 37.0 32.0 37.0 5 34.898507504987315 37.0 35.0 37.0 32.0 37.0 6 34.937089107258416 37.0 35.0 37.0 32.0 37.0 7 34.854911976016794 37.0 35.0 37.0 32.0 37.0 8 34.80465241508965 37.0 35.0 37.0 32.0 37.0 9 36.30714945891348 39.0 35.0 39.0 32.0 39.0 10-11 36.15934395719803 39.0 35.0 39.0 31.0 39.0 12-13 36.01335341381271 38.5 35.0 39.0 30.0 39.0 14-15 37.01563884108447 39.0 36.0 41.0 31.0 41.0 16-17 36.871675030034126 39.0 36.0 41.0 31.0 41.0 18-19 36.85342814090765 39.0 36.0 41.0 30.5 41.0 20-21 36.9136653277931 39.0 36.0 41.0 30.0 41.0 22-23 36.848100568247126 39.0 36.0 41.0 30.0 41.0 24-25 36.71984440515122 39.0 36.0 41.0 30.0 41.0 26-27 36.52799652344236 39.0 36.0 41.0 30.0 41.0 28-29 36.34607021449794 39.0 35.5 41.0 29.5 41.0 30-31 36.1036592028795 39.0 35.0 40.0 29.0 41.0 32-33 35.814115957682986 38.0 35.0 40.0 27.0 41.0 34-35 35.56458745006778 38.0 34.5 40.0 27.0 41.0 36-37 35.26282303408381 38.0 34.0 40.0 26.0 41.0 38-39 34.991153042539025 38.0 34.0 40.0 25.0 41.0 40-41 34.91003241952981 38.0 34.0 40.0 25.0 41.0 42-43 34.706251978000694 38.0 33.5 40.0 24.0 41.0 44-45 34.40843143954364 38.0 33.0 40.0 24.0 41.0 46-47 34.022520945157616 38.0 33.0 40.0 23.0 41.0 48-49 33.628816380128825 38.0 32.5 40.0 21.0 41.0 50-51 32.84157068606905 36.5 31.5 39.5 19.0 40.5 52-53 32.85719047536573 37.0 31.5 39.0 19.0 40.0 54-55 32.95584740305238 37.0 32.0 40.0 18.0 41.0 56-57 32.6308640536851 36.5 31.0 39.5 15.5 41.0 58-59 32.27423568478581 36.0 31.0 39.0 13.0 41.0 60-61 31.798684167019623 35.5 31.0 39.0 8.5 40.5 62-63 31.259764513604708 35.0 30.0 38.5 7.5 40.0 64-65 30.734811552626233 35.0 29.0 38.0 4.5 40.0 66-67 30.185115672693435 34.5 29.0 37.5 2.0 40.0 68-69 29.631841205710497 34.0 28.0 37.0 2.0 39.0 70-71 28.997132312304466 34.0 27.5 36.0 2.0 39.0 72-73 28.466411068301447 34.0 26.0 36.0 2.0 39.0 74-75 27.882910388892647 33.0 26.0 35.0 2.0 37.5 76-77 27.18289180946323 33.0 25.0 35.0 2.0 37.0 78-79 26.43290448441296 32.0 23.5 35.0 2.0 36.5 80-81 25.82777231066695 32.0 21.0 35.0 2.0 36.0 82-83 25.199284849420025 31.0 19.5 34.5 2.0 35.5 84-85 24.583224979570502 31.0 17.5 34.0 2.0 35.0 86-87 23.94643456025797 31.0 9.5 34.0 2.0 35.0 88-89 23.16310204043073 30.0 4.5 34.0 2.0 35.0 90-91 22.36339395790657 29.5 2.0 34.0 2.0 35.0 92-93 21.548644567642505 29.0 2.0 34.0 2.0 35.0 94-95 20.599378691283885 28.0 2.0 34.0 2.0 35.0 96-97 19.388503426960014 26.0 2.0 33.5 2.0 35.0 98-99 17.930652437133038 24.0 2.0 33.0 2.0 34.5 100-101 14.527293339274552 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5505517562684474 1101 2 0.6022229604152827 1101 3 0.5381310975148814 1101 4 0.41989714727858285 1101 5 0.42390579253846283 1101 6 0.4767891880163049 1101 7 0.39505233345341395 1101 8 0.2968037461916353 1101 9 0.2836862520349541 1101 10-11 0.5050771883575464 1101 12-13 0.5677887354578743 1101 14-15 0.6874335456818486 1101 16-17 0.6209685415684802 1101 18-19 0.47866643123741426 1101 20-21 0.7502558371954677 1101 22-23 0.7047755537311104 1101 24-25 0.6943544857923527 1101 26-27 0.7941106201044832 1101 28-29 0.760148152450661 1101 30-31 0.6743513681989697 1101 32-33 0.794804284582419 1101 34-35 0.736731111891892 1101 36-37 0.741260888803815 1101 38-39 0.7078252698552774 1101 40-41 0.773687373541712 1101 42-43 0.7818380909115277 1101 44-45 0.8611791838275451 1101 46-47 0.8105425117259273 1101 48-49 0.6874899170853439 1101 50-51 0.7744811742056612 1101 52-53 0.7875201559868827 1101 54-55 0.8978096833810127 1101 56-57 0.7460478895173139 1101 58-59 0.763061186478744 1101 60-61 0.7624326684345739 1101 62-63 0.6494010847848095 1101 64-65 0.6940545793406621 1101 66-67 0.7174394627363831 1101 68-69 0.7216784933128544 1101 70-71 0.8283740149149814 1101 72-73 0.8138295467802941 1101 74-75 0.6034194235244001 1101 76-77 0.795162785945223 1101 78-79 0.6233415950214365 1101 80-81 0.7392772704006596 1101 82-83 0.4079956362968069 1101 84-85 0.4937500056222781 1101 86-87 0.5363042565586049 1101 88-89 0.7218184491730071 1101 90-91 0.6518236794346919 1101 92-93 0.7183134869640888 1101 94-95 0.5331584325499144 1101 96-97 0.5154717397465447 1101 98-99 0.5054718120945765 1101 100-101 0.3405823630270497 1102 1 0.4904698369998748 1102 2 0.5754944217379254 1102 3 0.5743953499468866 1102 4 0.5375819651482274 1102 5 0.6235175013533194 1102 6 0.5415704877608789 1102 7 0.5060145930846858 1102 8 0.5682598206419769 1102 9 0.6635500914592924 1102 10-11 0.7090949412394565 1102 12-13 0.6652306545365079 1102 14-15 0.7288140349307426 1102 16-17 0.6738796195882202 1102 18-19 0.71161021548577 1102 20-21 0.6187079843742467 1102 22-23 0.7772160889116719 1102 24-25 0.7699261294146709 1102 26-27 0.7329091491614079 1102 28-29 0.6207556905778375 1102 30-31 0.7390343893128204 1102 32-33 0.6630288851794433 1102 34-35 0.7364850092050332 1102 36-37 0.7015964956453047 1102 38-39 0.8721016561056629 1102 40-41 0.8974742135019937 1102 42-43 0.96301577755127 1102 44-45 0.9881622972720692 1102 46-47 0.6978890224346728 1102 48-49 0.8106784632326196 1102 50-51 0.8855012888986309 1102 52-53 0.914712322386535 1102 54-55 0.7384448544851736 1102 56-57 0.7838634823186439 1102 58-59 0.8457626854746536 1102 60-61 0.8671486431917721 1102 62-63 0.7193249247814499 1102 64-65 0.7248737082983432 1102 66-67 0.7469281751888524 1102 68-69 0.6540737915831905 1102 70-71 0.6310497053051769 1102 72-73 0.6907546489669514 1102 74-75 0.6325946815011179 1102 76-77 0.6001319359519037 1102 78-79 0.6966645136106173 1102 80-81 0.7442377483959284 1102 82-83 0.8679886112178288 1102 84-85 0.7063365554825332 1102 86-87 0.8298150429038635 1102 88-89 1.0733351827187079 1102 90-91 1.0855118446507568 1102 92-93 1.2277351150355145 1102 94-95 0.9466335636828482 1102 96-97 0.948025295977228 1102 98-99 0.7166059874793831 1102 100-101 0.35807727134427125 1103 1 0.6564932949378495 1103 2 0.6113744628713818 1103 3 0.48617829583169936 1103 4 0.6309145025456502 1103 5 0.6328589018719342 1103 6 0.6510685791895128 1103 7 0.5549817585750745 1103 8 0.5122102212338078 1103 9 0.5842565715100889 1103 10-11 0.5941672190788267 1103 12-13 0.6506972675771934 1103 14-15 0.8337575864860938 1103 16-17 0.8423709773033536 1103 18-19 0.7802744021501269 1103 20-21 0.6684790012693043 1103 22-23 0.7174414017790838 1103 24-25 0.7957849745674892 1103 26-27 0.7297954287416317 1103 28-29 0.840856413954377 1103 30-31 0.7912736648739624 1103 32-33 0.9199441366672758 1103 34-35 0.9782571812439542 1103 36-37 1.007973820468024 1103 38-39 0.9332996369486466 1103 40-41 0.948889958581745 1103 42-43 0.988074898522207 1103 44-45 0.9993613667448358 1103 46-47 0.9757773247129506 1103 48-49 1.0950562550894816 1103 50-51 1.125465557916037 1103 52-53 1.1052377262203308 1103 54-55 1.1807406574465347 1103 56-57 1.339387309433711 1103 58-59 1.1521126151085 1103 60-61 1.2358657540114173 1103 62-63 1.2676155248102567 1103 64-65 1.0734199449171697 1103 66-67 1.0947342033745535 1103 68-69 1.1710101194220144 1103 70-71 1.30848415663338 1103 72-73 1.299324115270391 1103 74-75 1.0833263483775362 1103 76-77 0.9788210151529491 1103 78-79 1.2767115806984428 1103 80-81 1.1250617980699822 1103 82-83 1.0914415965277833 1103 84-85 1.0693347112719174 1103 86-87 1.1016754465917096 1103 88-89 1.0512924036359053 1103 90-91 1.1827723018369838 1103 92-93 1.2334337330259935 1103 94-95 1.1418741700041721 1103 96-97 0.9983431399830422 1103 98-99 1.2312286779692592 1103 100-101 0.9364543085773231 1104 1 0.5127970153508201 1104 2 0.5466627802433663 1104 3 0.558456874303463 1104 4 0.5534542258222075 1104 5 0.4722386379355825 1104 6 0.4669572144377554 1104 7 0.4184757409883275 1104 8 0.38896878385678235 1104 9 0.5550121804952752 1104 10-11 0.5426145331144596 1104 12-13 0.5218311988420794 1104 14-15 0.6039996857301233 1104 16-17 0.6513985956177066 1104 18-19 0.6826483435006026 1104 20-21 0.6585736212360729 1104 22-23 0.6824663456104432 1104 24-25 0.7129931790168555 1104 26-27 0.6710252406723427 1104 28-29 0.6433467448428587 1104 30-31 0.625065577489508 1104 32-33 0.45920016663588825 1104 34-35 0.6466398619403932 1104 36-37 0.5871275987380784 1104 38-39 0.5987830474998077 1104 40-41 0.5644339277126278 1104 42-43 0.5615926766301627 1104 44-45 0.5360174300319258 1104 46-47 0.6278876448854831 1104 48-49 0.5068114858649437 1104 50-51 0.3971378752159751 1104 52-53 0.5911906120955521 1104 54-55 0.8950504922499363 1104 56-57 0.8923810194459136 1104 58-59 0.7238726185303932 1104 60-61 0.853694965309078 1104 62-63 0.8911366797906943 1104 64-65 0.8758875381976452 1104 66-67 0.9314893215876019 1104 68-69 0.9425212473424445 1104 70-71 0.8257714924610973 1104 72-73 0.739788165086015 1104 74-75 0.5883604773020252 1104 76-77 0.764073312551858 1104 78-79 0.8150850087643065 1104 80-81 0.782483375834854 1104 82-83 0.6174849375603877 1104 84-85 0.46488928378237304 1104 86-87 0.6509512200080714 1104 88-89 0.6642565547576993 1104 90-91 0.7104212331694058 1104 92-93 0.8644657277670795 1104 94-95 0.6458746382638232 1104 96-97 0.6165283862020701 1104 98-99 0.37830340590589984 1104 100-101 0.09986296867934996 1105 1 0.4012684898612946 1105 2 0.5249767250630306 1105 3 0.4147751399012485 1105 4 0.49865320262472324 1105 5 0.46127141176727093 1105 6 0.44742160820170795 1105 7 0.41391988969669313 1105 8 0.385158864255402 1105 9 0.5154259854130956 1105 10-11 0.47480242668754613 1105 12-13 0.5227956544673873 1105 14-15 0.6984759949438768 1105 16-17 0.6268505272254785 1105 18-19 0.6641077005865839 1105 20-21 0.6466142340125032 1105 22-23 0.7742155734008875 1105 24-25 0.6379899273299472 1105 26-27 0.7915661733301746 1105 28-29 0.7112558731475502 1105 30-31 0.5976433351050616 1105 32-33 0.7000635080164912 1105 34-35 0.6709355148315339 1105 36-37 0.789547276784063 1105 38-39 0.7367096661789674 1105 40-41 0.7247207846584942 1105 42-43 0.7075670353604906 1105 44-45 0.5910520459574755 1105 46-47 0.5118039025798211 1105 48-49 0.6243553677763671 1105 50-51 0.6026595039568718 1105 52-53 0.5535785875159647 1105 54-55 0.508936464313976 1105 56-57 0.6309700305418247 1105 58-59 0.6935870939830622 1105 60-61 0.7305242374705152 1105 62-63 0.750605539204674 1105 64-65 0.8037347205448455 1105 66-67 0.6525324415996998 1105 68-69 0.4506211730030678 1105 70-71 0.6787243942876451 1105 72-73 0.715774060695761 1105 74-75 0.509605519140397 1105 76-77 0.641268729946848 1105 78-79 0.7280625705384267 1105 80-81 0.7749716089580723 1105 82-83 0.8003971906568914 1105 84-85 0.6952403179245543 1105 86-87 0.7855393872515783 1105 88-89 0.7553501049970563 1105 90-91 0.8678654284059775 1105 92-93 0.9355833986498361 1105 94-95 0.9524494963263699 1105 96-97 1.0319000355105459 1105 98-99 0.9314663191212844 1105 100-101 0.7891906678702867 1106 1 0.2343535480008896 1106 2 0.3060307617699536 1106 3 0.13427378506802157 1106 4 0.3742231078222247 1106 5 0.2637041742655697 1106 6 -0.0032111762032229763 1106 7 0.14633137000170393 1106 8 0.21907770529205806 1106 9 0.2730585538227004 1106 10-11 -0.04702896797685696 1106 12-13 0.04309829360267514 1106 14-15 0.4262236653165914 1106 16-17 0.12183902624223464 1106 18-19 -0.24637610846535551 1106 20-21 0.14028367722569612 1106 22-23 0.17490721909076257 1106 24-25 0.38147608162021385 1106 26-27 0.15720761290318563 1106 28-29 0.2107235623968151 1106 30-31 0.2283184586881788 1106 32-33 0.25552235476525453 1106 34-35 0.10757681825582921 1106 36-37 -0.08273975540947731 1106 38-39 0.19606001161794495 1106 40-41 0.17985901654738257 1106 42-43 -0.2304285382708784 1106 44-45 0.20168398071255922 1106 46-47 -0.10369693529777635 1106 48-49 0.24960875281208672 1106 50-51 -0.14471410550925157 1106 52-53 0.2552069977596787 1106 54-55 0.08805969732379992 1106 56-57 -0.4583206960930539 1106 58-59 -0.786709525946037 1106 60-61 0.11636538570575183 1106 62-63 0.14858403794503872 1106 64-65 0.21440386586874993 1106 66-67 -0.3873357398322774 1106 68-69 -0.4750928143832134 1106 70-71 -0.5331720856027822 1106 72-73 -0.04679963107379237 1106 74-75 -0.11953869919709348 1106 76-77 -0.17877087602035502 1106 78-79 0.11699245190478891 1106 80-81 -0.057045193635513414 1106 82-83 -0.013140648959925727 1106 84-85 -0.03876005878766975 1106 86-87 0.14221958007091118 1106 88-89 0.10210648991095894 1106 90-91 -0.14186843669214966 1106 92-93 -0.07443177220656239 1106 94-95 -0.2427413319593974 1106 96-97 -0.06321043432534879 1106 98-99 -0.31628057325879055 1106 100-101 -0.38008937160260103 1107 1 0.3961425109558867 1107 2 0.4633398073637309 1107 3 0.4315383134755848 1107 4 0.44837009593514665 1107 5 0.3829564522101876 1107 6 0.4480083950790288 1107 7 0.2947488005053742 1107 8 0.36942776697074464 1107 9 0.43874446637172326 1107 10-11 0.39592672511619753 1107 12-13 0.33918826211892394 1107 14-15 0.4283697250998486 1107 16-17 0.46666329770211235 1107 18-19 0.5042209102204254 1107 20-21 0.47420574404799254 1107 22-23 0.5307792433242327 1107 24-25 0.4720024193403489 1107 26-27 0.454595062622694 1107 28-29 0.6342080739239009 1107 30-31 0.5230742682584975 1107 32-33 0.490754354811763 1107 34-35 0.6526684447804811 1107 36-37 0.7027013126153818 1107 38-39 0.6280728008609486 1107 40-41 0.6087921351439292 1107 42-43 0.7284108461632215 1107 44-45 0.873781133608837 1107 46-47 0.7645723597627949 1107 48-49 0.7297402209586608 1107 50-51 0.6952630835511897 1107 52-53 0.5997336320026392 1107 54-55 0.6765267517958122 1107 56-57 0.6500791368463652 1107 58-59 0.7291816753687428 1107 60-61 0.8018911953051493 1107 62-63 0.7921886412197701 1107 64-65 0.778759786295975 1107 66-67 0.750261562778963 1107 68-69 0.6247922587930006 1107 70-71 0.6202020729167756 1107 72-73 0.6724654865033237 1107 74-75 0.8753094588243826 1107 76-77 0.8490192730259736 1107 78-79 0.766656344241806 1107 80-81 0.676332569671434 1107 82-83 0.9137418107454316 1107 84-85 0.8345714430811064 1107 86-87 0.6988944447218053 1107 88-89 0.7753062038536385 1107 90-91 0.7476823796662195 1107 92-93 0.8226639998235861 1107 94-95 0.7675769358821221 1107 96-97 1.0082268099115943 1107 98-99 0.9398979548057405 1107 100-101 0.7212673657159581 1108 1 0.3186410338518222 1108 2 0.3554126455563775 1108 3 0.27238470602254594 1108 4 0.3233146701495855 1108 5 0.2809986795987598 1108 6 0.3179417264318829 1108 7 0.2727390330974657 1108 8 0.2756948325196902 1108 9 0.27264480228224386 1108 10-11 0.35745614121722724 1108 12-13 0.384220406323891 1108 14-15 0.38146635319494493 1108 16-17 0.20548879000595122 1108 18-19 0.28773801510379826 1108 20-21 0.3237827751623499 1108 22-23 0.39067826111880066 1108 24-25 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-1.0118395364058799 2316 54-55 -1.0492561029528247 2316 56-57 -0.7919125766255419 2316 58-59 -0.814456120822264 2316 60-61 -0.8186812991539121 2316 62-63 -0.915606496560013 2316 64-65 -1.0743402373584416 2316 66-67 -1.0162048758614155 2316 68-69 -0.9541363745053992 2316 70-71 -1.0465561035405777 2316 72-73 -0.8953976562882069 2316 74-75 -0.7785110173833623 2316 76-77 -0.6577196460880046 2316 78-79 -0.6369685945358441 2316 80-81 -0.7414360993330469 2316 82-83 -0.47923633798272647 2316 84-85 -0.49189951925081843 2316 86-87 -0.5766239812637544 2316 88-89 -0.8112180298381944 2316 90-91 -0.749292329675086 2316 92-93 -0.6459915947339461 2316 94-95 -0.8313088892513001 2316 96-97 -0.9348388486509869 2316 98-99 -0.7904497421115302 2316 100-101 -0.611785970679211 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9896.0 3 3678.0 4 3847.0 5 4303.0 6 5273.0 7 6573.0 8 8636.0 9 10762.0 10 13374.0 11 16621.0 12 20154.0 13 23716.0 14 26342.0 15 31514.0 16 36261.0 17 41402.0 18 39911.0 19 37882.0 20 39122.0 21 42352.0 22 46915.0 23 51926.0 24 58056.0 25 65027.0 26 73641.0 27 84328.0 28 96415.0 29 110759.0 30 128472.0 31 149714.0 32 177687.0 33 214519.0 34 264940.0 35 330311.0 36 386113.0 37 358012.0 38 149520.0 39 7581.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.66614272648082 17.433830992376574 14.28921182758687 23.610814453555733 2 16.34772488546136 19.227243026075932 38.68419344695936 25.740838641503345 3 19.771835326489843 22.864858853392338 28.80762519017136 28.555680629946462 4 13.54892169063785 15.516312481293026 34.71708754076151 36.21767828730761 5 14.680685542301921 36.70231252211521 32.93878069307289 15.678221242509974 6 34.44695164279261 34.22057904622898 16.34042102656065 14.99204828441776 7 30.710624718449843 31.878250624529436 18.917429805411434 18.493694851609284 8 28.317195482262747 33.14872223942571 18.783708903135405 19.75037337517613 9 27.325192361490906 16.157168096093795 18.84887431038437 37.66876523203093 10-11 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25.390586971350075 27.18866227368892 23.446024817226185 36-37 24.13685170937828 24.62001563918719 27.250606317803154 23.99252633363137 38-39 23.323910495164395 25.107887008468584 27.221828225896754 24.34637427047026 40-41 23.843820073598636 24.888110603955475 27.596119046726596 23.671950275719283 42-43 23.498423089509867 24.901279702313065 27.277433250212056 24.322863957965012 44-45 23.33328294628205 25.303227700029506 27.532996432911688 23.830492920776756 46-47 24.18593972403765 25.747567972141418 26.485620734197862 23.58087156962307 48-49 23.651963505308245 25.80593704053113 26.382892550283817 24.15920690387681 50-51 23.9201424756242 25.952246730996954 26.522684613640372 23.60492617973848 52-53 24.438165003550886 25.272646535013614 26.589103586629776 23.700084874805725 54-55 23.552594925935182 25.84125127490945 26.751674802309317 23.85447899684605 56-57 23.56503422439532 26.101854836411185 26.47482823241379 23.858282706779704 58-59 23.570808481462997 25.241679304856767 26.53152267213357 24.65598954154667 60-61 22.896019218759204 25.81046859267877 27.029639733298144 24.263872455263886 62-63 23.324058478351684 25.78372419622341 27.200476533661643 23.69174079176326 64-65 22.973296623440426 25.938625614991746 26.807837567384357 24.280240194183474 66-67 22.88925408814004 25.910445984495674 26.825407097703607 24.374892829660688 68-69 23.70632533155964 25.294964626919818 27.145422033095496 23.853288008425043 70-71 23.523299350422118 25.560478652249518 26.408996258383517 24.507225738944843 72-73 23.375361876290473 25.768662800668547 26.394909770892127 24.46106555214885 74-75 23.358513041782903 26.317792845216765 26.347006262537754 23.97668785046258 76-77 23.727128339552962 25.74291461572209 26.459476253660046 24.0704807910649 78-79 23.727258169328984 26.080332909397043 26.455876325443878 23.7365325958301 80-81 24.04764769089876 26.08447955940577 26.064182571485077 23.80369017821039 82-83 23.791322016369378 26.17171390956139 26.170958094628343 23.86600597944089 84-85 23.630644958115514 26.348271083957925 26.23781255904768 23.78327139887888 86-87 24.129738002990248 26.37187246334821 25.684036818307433 23.814352715354108 88-89 23.831736546751486 26.520118250932008 25.496589339461128 24.15155586285538 90-91 23.649831889543886 26.471372079330873 25.669976792472028 24.208819238653216 92-93 23.90546010047719 25.85411909224058 25.966297620360017 24.274123186922218 94-95 23.698947818050204 25.650234710727137 26.29917789819395 24.35163957302871 96-97 24.239552335309384 25.21287683345937 26.09402728899791 24.453543542233337 98-99 23.78500851289738 25.756369081937834 25.66675963581083 24.791862769353948 100-101 24.444996896790745 24.929923591846876 26.062795965528444 24.562283545833935 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 428.0 1 321.5 2 314.0 3 435.0 4 387.5 5 389.0 6 682.0 7 1450.0 8 2378.0 9 2738.5 10 2376.0 11 1994.5 12 2030.5 13 2154.5 14 2336.0 15 2586.5 16 2860.5 17 3194.5 18 3520.5 19 3839.0 20 4333.5 21 5182.5 22 6119.0 23 7152.5 24 8579.5 25 10534.0 26 13029.5 27 16080.5 28 18860.0 29 21633.0 30 24890.0 31 31848.5 32 43727.0 33 46183.5 34 41663.5 35 42882.5 36 46872.0 37 52616.5 38 59733.0 39 64649.0 40 69412.0 41 76722.5 42 81821.5 43 87047.0 44 92927.5 45 100589.0 46 106654.5 47 111745.0 48 115462.0 49 114693.5 50 115799.0 51 117437.0 52 120999.0 53 128781.0 54 136213.5 55 132208.5 56 124680.0 57 119897.5 58 115459.5 59 104646.0 60 84032.0 61 69352.5 62 59802.0 63 45560.5 64 32787.5 65 25837.5 66 19826.5 67 15192.5 68 11798.5 69 9160.5 70 7293.5 71 5163.0 72 3681.0 73 2931.5 74 2158.0 75 1546.5 76 1136.5 77 801.5 78 525.5 79 331.0 80 229.0 81 154.0 82 93.5 83 54.5 84 34.5 85 24.0 86 17.0 87 12.0 88 11.5 89 9.5 90 7.0 91 7.5 92 4.5 93 2.5 94 1.0 95 1.5 96 1.5 97 0.0 98 0.0 99 1.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18837652000988803 2 0.04777117700685392 3 0.045472366247789754 4 0.05054234614106825 5 0.057753684001694186 6 0.06379987120361637 7 0.03668650046999659 8 0.0357102931613529 9 0.04166200868824505 10-11 0.04211862178422354 12-13 0.03885934899568737 14-15 0.03391533133578225 16-17 0.0321203695102116 18-19 0.04074878249628805 20-21 0.050558091420239926 22-23 0.04740903558590546 24-25 0.06740554013392934 26-27 0.0523373079666389 28-29 0.04490553619760956 30-31 0.05397481700049283 32-33 0.04981806329917133 34-35 0.0488103654321843 36-37 0.04651155467312013 38-39 0.025869493679057675 40-41 0.024184748807688734 42-43 0.02128761744010102 44-45 0.00428271593469488 46-47 0.011824704657925938 48-49 0.011131912374372355 50-51 0.008659903544419794 52-53 0.009132261919569966 54-55 0.01037613897413208 56-57 0.00823478100678464 58-59 0.010218686182415357 60-61 0.010202940903243685 62-63 0.0038890839554030715 64-65 0.006534290856244026 66-67 0.0037631217220296925 68-69 0.00815605461092628 70-71 0.022799164240581565 72-73 0.0191934953102686 74-75 0.02027991957311399 76-77 0.012344298870591125 78-79 0.0049282723807334465 80-81 0.006518545577072354 82-83 0.005495102430913651 84-85 0.0048810365432184295 86-87 0.007321554814827644 88-89 0.02991603042617747 90-91 0.0363401043282198 92-93 0.02220084363205802 94-95 0.03177397336843481 96-97 0.035442623415434464 98-99 0.020988457135839248 100-101 0.12087650820092866 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3175555.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.228655862100986 #Duplication Level Percentage of deduplicated Percentage of total 1 71.82460680076386 28.894073894186473 2 13.507119751500923 10.867465443426353 3 5.34883582385281 6.455294268619563 4 2.673161045171667 4.301507030007408 5 1.6160180606042365 3.250511721349384 6 0.981572485324053 2.36924050294851 7 0.680376894611867 1.9159453564906024 8 0.4935757175975064 1.5884710148095702 9 0.38097898907758326 1.3793645378063906 >10 2.176298216502178 16.192510324148618 >50 0.1863314668043681 5.153238773763439 >100 0.11549131369377698 8.707479789491973 >500 0.009316895305423824 2.4961138354182304 >1k 0.005684885270995718 4.794279189217655 >5k 6.316539189995242E-4 1.6345043183158072 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9541 0.3004514171538518 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7317 0.2304164153982532 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6431 0.2025157807060498 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6366 0.2004688944137324 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5945 0.1872113693511843 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5517 0.17373341038023274 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5332 0.16790765708671399 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5010 0.15776769730015697 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4919 0.1549020564909126 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4711 0.1483520203554969 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4665 0.14690345467170304 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4431 0.13953466401936038 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 4013 0.1263716106318423 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3825 0.12045138566329351 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3500 0.11021695420170648 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3469 0.10924074689306279 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3313 0.104328219791501 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3286 0.1034779747162307 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3284 0.10341499359954402 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3270 0.1029741257827372 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.149055834334471E-5 7 0.0 0.0 0.0 0.0 3.149055834334471E-5 8 0.0 0.0 0.0 0.0 3.149055834334471E-5 9 0.0 0.0 0.0 0.0 3.149055834334471E-5 10-11 0.0 0.0 0.0 0.0 3.149055834334471E-5 12-13 0.0 0.0 0.0 3.149055834334471E-5 1.7319807088839588E-4 14-15 0.0 0.0 0.0 3.149055834334471E-5 1.8894335006006824E-4 16-17 0.0 0.0 0.0 4.723583751501706E-5 1.8894335006006824E-4 18-19 0.0 0.0 0.0 9.447167503003412E-5 1.8894335006006824E-4 20-21 0.0 0.0 0.0 1.8894335006006824E-4 1.8894335006006824E-4 22-23 0.0 0.0 0.0 5.195942126651876E-4 1.8894335006006824E-4 24-25 0.0 0.0 0.0 9.762073086436859E-4 2.046886292317406E-4 26-27 0.0 0.0 0.0 0.001731980708883959 2.2043390840341295E-4 28-29 0.0 0.0 0.0 0.0048023101473600675 2.5192446674675767E-4 30-31 0.0 0.0 0.0 0.015430373588238906 2.5192446674675767E-4 32-33 0.0 0.0 0.0 0.03682820798254163 2.834150250901024E-4 34-35 0.0 0.0 0.0 0.06666551201286075 2.834150250901024E-4 36-37 0.0 0.0 0.0 0.11694018840801057 2.834150250901024E-4 38-39 0.0 0.0 0.0 0.19725685746271124 2.834150250901024E-4 40-41 0.0 0.0 0.0 0.28154133686867333 2.834150250901024E-4 42-43 0.0 0.0 0.0 0.3696204285550085 2.991603042617747E-4 44-45 0.0 0.0 0.0 0.4826557877284443 3.1490558343344704E-4 46-47 0.0 0.0 0.0 0.6121134730779344 3.1490558343344704E-4 48-49 0.0 0.0 0.0 0.7517268634931531 3.1490558343344704E-4 50-51 0.0 0.0 0.0 0.9088332590680999 3.1490558343344704E-4 52-53 0.0 0.0 0.0 1.0582087225697556 3.1490558343344704E-4 54-55 0.0 0.0 0.0 1.2149214861654105 3.1490558343344704E-4 56-57 0.0 0.0 0.0 1.397550979277638 3.1490558343344704E-4 58-59 0.0 0.0 0.0 1.5962406571449717 3.1490558343344704E-4 60-61 0.0 0.0 0.0 1.8193355177283972 3.1490558343344704E-4 62-63 0.0 0.0 0.0 2.0299128813703433 3.4639614177679176E-4 64-65 0.0 0.0 0.0 2.248441610993984 3.4639614177679176E-4 66-67 0.0 0.0 0.0 2.5106005092023285 3.621414209484641E-4 68-69 1.5745279171672354E-5 0.0 0.0 2.8006915326612196 3.778867001201365E-4 70-71 3.149055834334471E-5 0.0 0.0 3.101111459256728 4.093772584634812E-4 72-73 3.149055834334471E-5 0.0 0.0 3.4167885613695876 4.093772584634812E-4 74-75 3.149055834334471E-5 0.0 0.0 3.7324184276449315 4.093772584634812E-4 76-77 3.149055834334471E-5 0.0 0.0 4.072721146382285 4.093772584634812E-4 78-79 3.149055834334471E-5 0.0 0.0 4.459787344259507 4.093772584634812E-4 80-81 3.149055834334471E-5 0.0 0.0 4.860929821716204 4.093772584634812E-4 82-83 3.149055834334471E-5 0.0 0.0 5.2760698523565175 4.093772584634812E-4 84-85 3.149055834334471E-5 0.0 0.0 5.702105616183628 4.093772584634812E-4 86-87 3.149055834334471E-5 0.0 0.0 6.1376987644679435 4.093772584634812E-4 88-89 3.149055834334471E-5 0.0 0.0 6.596594925926334 4.093772584634812E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5880 0.0 51.492825 1 GTATCAA 10775 0.0 43.828976 1 TATCAAC 13830 0.0 33.709045 2 AGGTAAG 1985 0.0 33.51716 2 ATCAACG 14070 0.0 33.134052 3 TCAACGC 14435 0.0 32.29623 4 CAACGCA 14635 0.0 31.853369 5 TAAGGTG 2195 0.0 31.824522 5 AACGCAG 15180 0.0 30.923521 6 AAGGTAA 2150 0.0 29.671074 1 GTAAGGT 2525 0.0 27.478376 4 ACGCAGA 17115 0.0 27.341486 7 CGCAGAG 17330 0.0 26.83822 8 TAAGGTA 1055 0.0 26.576584 4 GTGTAAG 1010 0.0 25.924372 1 GGTAAGG 2680 0.0 25.889143 3 TGTAAGG 1205 0.0 24.057053 2 TACATGG 13150 0.0 23.779339 2 GTACATG 13435 0.0 23.670406 1 GCAGAGT 19275 0.0 23.537004 9 >>END_MODULE