FastQCFastQC Report
Thu 26 May 2016
SRR938374_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938374_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3175555
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91440.287949665491544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77160.24298114817724775No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA63380.19958715878011876No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA63340.19946119654674538No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA60690.191116198585759No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC59860.1885024822432614No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA56430.1777012207314942No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG54540.17174950520460203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50990.16057035699271466No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA46510.14646258685489624No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA44480.14007000351119725No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA43010.13544089143472557No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA40700.12816657245741297No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG40280.12684396900699246No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT36980.11645208475368873No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT36240.11412178343628122No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG35670.11232682161071057No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT33410.10520995542511466No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG32950.10376138974132082No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA32750.10313157857445392No Hit
CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT31780.10007699441514947No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59950.051.092541
GTATCAA100250.042.0823551
AGGTAAG19450.033.0122382
ATCAACG136450.030.6241423
TATCAAC136950.030.561982
TAAGGTG23850.030.4956535
TCAACGC138600.030.1138444
CAACGCA141650.029.4654355
AAGGTAA21700.029.370131
AACGCAG147150.028.3709286
GTAAGGT26200.026.8531534
TAAGGTA10700.026.6564564
GTGTAAG10300.025.8589951
TGGTATC28000.024.9700892
GGTAAGG27550.024.8478893
ACGCAGA168050.024.7294527
CGCAGAG171000.024.2472828
GTACATG138500.024.244641
TACATGG135000.024.1685812
ACATGGG133700.023.6094173
Produced by FastQC (version 0.11.5)