##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938374_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3175555 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.828911166709442 31.0 31.0 34.0 30.0 34.0 2 32.052031534645124 33.0 31.0 34.0 30.0 34.0 3 32.113240362708254 33.0 31.0 34.0 30.0 34.0 4 35.779834706059255 37.0 35.0 37.0 35.0 37.0 5 35.642649552597895 37.0 35.0 37.0 33.0 37.0 6 35.67645970546881 37.0 35.0 37.0 33.0 37.0 7 35.61506697254496 37.0 35.0 37.0 33.0 37.0 8 35.59968194536073 37.0 35.0 37.0 33.0 37.0 9 37.19172491107854 39.0 37.0 39.0 33.0 39.0 10-11 37.098284079475874 39.0 37.0 39.0 33.0 39.0 12-13 36.99221380199681 39.0 37.0 39.0 33.0 39.0 14-15 38.061103334692675 40.0 37.0 41.0 33.0 41.0 16-17 37.983784724245055 40.0 37.0 41.0 33.0 41.0 18-19 38.08231568969833 40.0 37.5 41.0 33.0 41.0 20-21 38.119841571000975 40.0 37.0 41.0 33.0 41.0 22-23 38.03019330479239 40.0 37.0 41.0 33.0 41.0 24-25 37.9176246356936 40.0 37.0 41.0 32.5 41.0 26-27 37.71952792503987 40.0 37.0 41.0 32.0 41.0 28-29 37.60102989871062 40.0 37.0 41.0 32.0 41.0 30-31 37.35772628721594 39.0 36.0 41.0 31.5 41.0 32-33 37.13418583523195 39.0 36.0 41.0 31.0 41.0 34-35 36.98277041336081 39.0 36.0 41.0 31.0 41.0 36-37 36.97334324236236 39.0 36.0 41.0 31.0 41.0 38-39 36.84122098341865 39.0 35.0 41.0 30.0 41.0 40-41 36.651672857185595 39.0 35.0 41.0 30.0 41.0 42-43 36.41955547927843 39.0 35.0 40.5 30.0 41.0 44-45 36.14372416790136 39.0 35.0 40.0 29.5 41.0 46-47 35.76115308977486 38.0 35.0 40.0 28.5 41.0 48-49 35.37265281187068 38.0 34.0 40.0 27.0 41.0 50-51 34.979227410641606 38.0 34.0 40.0 26.0 41.0 52-53 34.4670504840886 38.0 33.0 40.0 25.0 41.0 54-55 34.122185728164055 37.0 33.0 40.0 24.0 41.0 56-57 33.75201327012128 37.0 33.0 40.0 23.0 41.0 58-59 33.28228435659278 36.0 32.0 39.0 22.5 41.0 60-61 32.88459907008381 36.0 31.5 39.0 22.0 40.0 62-63 32.406776925608284 35.0 31.0 38.5 20.5 40.0 64-65 31.877992193490584 35.0 31.0 38.0 19.0 40.0 66-67 31.37809249091891 35.0 30.0 37.5 18.0 40.0 68-69 30.83596237508089 34.0 29.5 37.0 14.5 39.0 70-71 30.251428962811225 34.0 29.0 36.0 11.0 39.0 72-73 29.824838492798897 34.0 29.0 36.0 8.0 38.5 74-75 29.336803645347032 34.0 28.0 35.0 7.0 37.5 76-77 28.09382186735862 31.5 26.5 34.5 6.5 36.0 78-79 28.80079513659817 33.0 28.0 35.0 2.0 36.0 80-81 28.571193854302635 33.0 27.5 35.0 2.0 36.0 82-83 28.13998104268388 33.0 27.0 35.0 2.0 36.0 84-85 27.660170741807338 33.0 26.0 35.0 2.0 35.0 86-87 27.13781291774194 32.5 25.5 35.0 2.0 35.0 88-89 26.513909222167463 32.0 24.5 34.0 2.0 35.0 90-91 26.000421658576215 32.0 24.0 34.0 2.0 35.0 92-93 25.297740709891656 31.0 21.0 34.0 2.0 35.0 94-95 24.567374364481168 31.0 18.5 34.0 2.0 35.0 96-97 23.606457611346677 31.0 4.5 34.0 2.0 35.0 98-99 22.389863189269278 30.5 2.0 34.0 2.0 35.0 100-101 18.722033313861672 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3294222210841582 1101 2 0.2451690606970658 1101 3 0.27203191534705695 1101 4 0.19776899733471964 1101 5 0.13198176803828687 1101 6 0.1086282277079178 1101 7 0.2850534658995727 1101 8 0.24162891797830355 1101 9 0.27951531160405807 1101 10-11 0.34511099446389437 1101 12-13 0.32184478361332225 1101 14-15 0.3954352844649591 1101 16-17 0.36057170300914265 1101 18-19 0.44041623219121107 1101 20-21 0.36450538903998364 1101 22-23 0.4033822692397493 1101 24-25 0.4266084526952838 1101 26-27 0.45691263942550364 1101 28-29 0.36026417540249867 1101 30-31 0.24510887455065244 1101 32-33 0.25666053615551476 1101 34-35 0.2986410384926401 1101 36-37 0.31277725222154373 1101 38-39 0.3990132875309058 1101 40-41 0.49234006983436274 1101 42-43 0.6106534817579998 1101 44-45 0.6596201486989273 1101 46-47 0.5775414864292898 1101 48-49 0.5161650073082527 1101 50-51 0.5539528445336614 1101 52-53 0.6775022821136361 1101 54-55 0.6272126621599625 1101 56-57 0.4586224321108361 1101 58-59 0.5449113743789482 1101 60-61 0.5288776482944755 1101 62-63 0.5763412321052641 1101 64-65 0.5509832893768305 1101 66-67 0.494631139092057 1101 68-69 0.3809356365266332 1101 70-71 0.3582679201751411 1101 72-73 0.30149781371331486 1101 74-75 0.467135742107299 1101 76-77 0.2880657110471354 1101 78-79 0.205565734226699 1101 80-81 0.35777893250086734 1101 82-83 0.400579915224629 1101 84-85 0.5900222406769657 1101 86-87 0.6505160804551373 1101 88-89 0.745272151421748 1101 90-91 0.787618029789062 1101 92-93 0.7098082181409922 1101 94-95 0.7683222225917667 1101 96-97 0.9069277941081175 1101 98-99 0.7718185282380716 1101 100-101 0.6641997126912571 1102 1 0.39624894092582963 1102 2 0.408725694991972 1102 3 0.37392280120052135 1102 4 0.2572938585794091 1102 5 0.30157657142916605 1102 6 0.2588215923196344 1102 7 0.31650487695704044 1102 8 0.3305578958293083 1102 9 0.3979641161847738 1102 10-11 0.48161101564409137 1102 12-13 0.4961972598911828 1102 14-15 0.5518864924673963 1102 16-17 0.6421652838213561 1102 18-19 0.6048247604021739 1102 20-21 0.5642516139972074 1102 22-23 0.4552499568379105 1102 24-25 0.4529767405241003 1102 26-27 0.5399438372989422 1102 28-29 0.43959564829365405 1102 30-31 0.47311760954035265 1102 32-33 0.5022204100839573 1102 34-35 0.5870309646143497 1102 36-37 0.5614302476829849 1102 38-39 0.6550557988804258 1102 40-41 0.7523960105557919 1102 42-43 0.6111850485667816 1102 44-45 0.6070292103510653 1102 46-47 0.5480793898043359 1102 48-49 0.5011409349791762 1102 50-51 0.5146292714200413 1102 52-53 0.4782429518603166 1102 54-55 0.3595863928630223 1102 56-57 0.47468152274672093 1102 58-59 0.6539388632805796 1102 60-61 0.7109075412426122 1102 62-63 0.8141623979859673 1102 64-65 0.7898947045303828 1102 66-67 0.7958861315902688 1102 68-69 0.8196259528763647 1102 70-71 0.7646230050540126 1102 72-73 0.5973647880572805 1102 74-75 0.6100077548399128 1102 76-77 0.7991409567405903 1102 78-79 0.6974010479816997 1102 80-81 0.6828780202277578 1102 82-83 0.5369517305877629 1102 84-85 0.5243396265478601 1102 86-87 0.44097366900868096 1102 88-89 0.7450849034520779 1102 90-91 0.4247993786667017 1102 92-93 0.46546448759912806 1102 94-95 0.48141372343328825 1102 96-97 0.31551835858483557 1102 98-99 0.24265401655790697 1102 100-101 -0.16720626402401706 1103 1 0.2563521191027007 1103 2 0.26088510580161284 1103 3 0.2830340582066313 1103 4 0.25847974725780887 1103 5 0.14324733341737073 1103 6 0.14175919876011278 1103 7 0.297475149947104 1103 8 0.27524748800227883 1103 9 0.3646045520577843 1103 10-11 0.41783280278659163 1103 12-13 0.25466572288470246 1103 14-15 0.42526521169327225 1103 16-17 0.48456333424094566 1103 18-19 0.41704240453077546 1103 20-21 0.4241688278897513 1103 22-23 0.4285345634214721 1103 24-25 0.49790634565534475 1103 26-27 0.5116746940433998 1103 28-29 0.48455300750271846 1103 30-31 0.6291557005766037 1103 32-33 0.6407405937585295 1103 34-35 0.5667907650338222 1103 36-37 0.4984495458589109 1103 38-39 0.5577255377959034 1103 40-41 0.5593741752743284 1103 42-43 0.6386008735591346 1103 44-45 0.7532376324695278 1103 46-47 0.6242862256337034 1103 48-49 0.6769751603508993 1103 50-51 0.655644690245829 1103 52-53 0.5869571700351273 1103 54-55 0.6134204033799051 1103 56-57 0.6987785342546218 1103 58-59 0.6733776040493566 1103 60-61 0.694324001229127 1103 62-63 0.6473640988324263 1103 64-65 0.6098947954489091 1103 66-67 0.5367854857337342 1103 68-69 0.4160362619850133 1103 70-71 0.2711554416754218 1103 72-73 0.17228949315824238 1103 74-75 0.32212158436192695 1103 76-77 0.35511148095291034 1103 78-79 0.34262940422729926 1103 80-81 0.3902744939286187 1103 82-83 0.49362845252491994 1103 84-85 0.26970379833029057 1103 86-87 0.5425950454865252 1103 88-89 0.5056650689525206 1103 90-91 0.4214934732210196 1103 92-93 0.34843917922979273 1103 94-95 0.3998953313322886 1103 96-97 0.47991910019293726 1103 98-99 0.44442795672298985 1103 100-101 0.07883830136865555 1104 1 0.3479387538916967 1104 2 0.2763788720950231 1104 3 0.27376814525789683 1104 4 0.22337130462636878 1104 5 0.13403545042825726 1104 6 0.10817732761256593 1104 7 0.13805345061482655 1104 8 0.12483956180719957 1104 9 0.25378437727086123 1104 10-11 0.3165370968176262 1104 12-13 0.2883171576052561 1104 14-15 0.5011392588524188 1104 16-17 0.47146725996775274 1104 18-19 0.38448316293130347 1104 20-21 0.35935054865391436 1104 22-23 0.40603434955287554 1104 24-25 0.4334734801314184 1104 26-27 0.436784329836442 1104 28-29 0.4797084336865325 1104 30-31 0.5498590806008252 1104 32-33 0.6126786709223211 1104 34-35 0.45725211335146554 1104 36-37 0.5647976608887006 1104 38-39 0.6028305360174215 1104 40-41 0.6882300008295985 1104 42-43 0.6546825533505896 1104 44-45 0.7920729041276644 1104 46-47 0.6684415347526098 1104 48-49 0.657269871268106 1104 50-51 0.7376922231027905 1104 52-53 0.7126530308899746 1104 54-55 0.6057938494557504 1104 56-57 0.5557041620152106 1104 58-59 0.5079644324457107 1104 60-61 0.3956995139509374 1104 62-63 0.3027684455426751 1104 64-65 0.35601949659569954 1104 66-67 0.34599095266514723 1104 68-69 0.3034638280704094 1104 70-71 0.27795432415575405 1104 72-73 0.08239140716956328 1104 74-75 0.10744603426939747 1104 76-77 0.08514673550155649 1104 78-79 0.10920444508745675 1104 80-81 0.2836581876868074 1104 82-83 0.1259250860081842 1104 84-85 0.3640453982474412 1104 86-87 0.4260905812611142 1104 88-89 0.31439495967692466 1104 90-91 0.1585991107970557 1104 92-93 0.08595848368411296 1104 94-95 0.3867914902524099 1104 96-97 0.24993596204283008 1104 98-99 0.1226772959816067 1104 100-101 -0.27889563710022003 1105 1 0.2357475187476723 1105 2 0.23306767138186757 1105 3 0.16471016159987073 1105 4 0.20707173490649922 1105 5 0.21205256646219084 1105 6 0.1770882398508533 1105 7 0.14797432094601248 1105 8 0.18512361648630105 1105 9 0.1805015058885644 1105 10-11 0.2934793056171685 1105 12-13 0.2435320174443376 1105 14-15 0.376351305850136 1105 16-17 0.32630614020310134 1105 18-19 0.3599482599056003 1105 20-21 0.3812731574479571 1105 22-23 0.44597992213000026 1105 24-25 0.47597535447777517 1105 26-27 0.33126592634598495 1105 28-29 0.3139040720904873 1105 30-31 0.3563320524734692 1105 32-33 0.3180819699834032 1105 34-35 0.48146195343191067 1105 36-37 0.4797156073453124 1105 38-39 0.3252231325343615 1105 40-41 0.41314574777116064 1105 42-43 0.2978594038776734 1105 44-45 0.27511966602910576 1105 46-47 -0.03000622094894112 1105 48-49 0.04839710158164934 1105 50-51 -0.0319827033773592 1105 52-53 0.10822275287338101 1105 54-55 0.15275238542469793 1105 56-57 0.06482617482527075 1105 58-59 0.23958917614404385 1105 60-61 0.08592309254076724 1105 62-63 -0.032096934619175954 1105 64-65 0.03531846462130517 1105 66-67 -0.00805821707079346 1105 68-69 -0.14873351918754807 1105 70-71 -0.21614907208582324 1105 72-73 0.08174931796865081 1105 74-75 0.01977785860851 1105 76-77 -0.029593875054210628 1105 78-79 -0.03285683890060653 1105 80-81 0.08086633689006462 1105 82-83 0.21544809790566077 1105 84-85 0.07027335683311264 1105 86-87 0.06502992857616974 1105 88-89 0.06135217382191982 1105 90-91 0.03844573692073894 1105 92-93 0.022157637082308668 1105 94-95 0.055202118562917235 1105 96-97 -0.0408599663164253 1105 98-99 0.015057298159973698 1105 100-101 -0.19694815686677813 1106 1 0.028180004853851415 1106 2 0.10025828879068399 1106 3 0.0875167086126396 1106 4 0.08217095455940893 1106 5 0.003178946247487602 1106 6 0.0075246425357491376 1106 7 0.11154747733379367 1106 8 0.07485063320188345 1106 9 0.028275883929516965 1106 10-11 -0.014410614566926938 1106 12-13 -0.04648234470918311 1106 14-15 0.05155417254825778 1106 16-17 -0.09605823060046959 1106 18-19 0.17827474656250786 1106 20-21 0.011894333383828837 1106 22-23 0.07492636655010187 1106 24-25 -0.04756491124766171 1106 26-27 -0.09913812822406243 1106 28-29 0.0909184844040638 1106 30-31 0.12626330249266005 1106 32-33 0.04101616920888773 1106 34-35 -0.11338338470313403 1106 36-37 -0.3825151534598277 1106 38-39 -0.14324175491774582 1106 40-41 0.12338853265722349 1106 42-43 0.21297344633764936 1106 44-45 0.13422750297413444 1106 46-47 -0.09352189801239064 1106 48-49 -0.010114854424912778 1106 50-51 -0.04072488378361783 1106 52-53 -0.06367210961129643 1106 54-55 -0.3135062275167968 1106 56-57 -0.11352843217073172 1106 58-59 -0.03905630796699455 1106 60-61 -0.4174670103692293 1106 62-63 -0.39414896185301274 1106 64-65 -0.6706325136532527 1106 66-67 -0.4023984230136328 1106 68-69 -1.007213741283973 1106 70-71 -0.8042180943640496 1106 72-73 -0.6533786874117844 1106 74-75 -0.365357875514551 1106 76-77 -0.3927911381580138 1106 78-79 -0.5044592375747428 1106 80-81 -1.112145753729699 1106 82-83 -0.7496914852336012 1106 84-85 -0.722708996041824 1106 86-87 -0.5174547956407913 1106 88-89 -0.34312409893820117 1106 90-91 -0.399924808687544 1106 92-93 -0.38606779242592637 1106 94-95 -0.12324973751253765 1106 96-97 -0.41255362557407693 1106 98-99 -0.47317356705996616 1106 100-101 -0.9934049634322157 1107 1 0.19588663330201683 1107 2 0.18706515455401984 1107 3 0.14343393804281845 1107 4 0.10764323500411876 1107 5 0.118927506512847 1107 6 0.1366745813415733 1107 7 0.12304190396425696 1107 8 0.1332858841464386 1107 9 0.2852077834877349 1107 10-11 0.19770539563113232 1107 12-13 0.12178457623065242 1107 14-15 0.3102391906836388 1107 16-17 0.2570989623554638 1107 18-19 0.2168112319529456 1107 20-21 0.2444684325988149 1107 22-23 0.2762807781993857 1107 24-25 0.35966215450385874 1107 26-27 0.24194278471741626 1107 28-29 0.24864944516712484 1107 30-31 0.24794149575772195 1107 32-33 0.3397849211287749 1107 34-35 0.31452905578638024 1107 36-37 0.3743948944974065 1107 38-39 0.3435415634440915 1107 40-41 0.30929865910579935 1107 42-43 0.36249808922628546 1107 44-45 0.43236110492226487 1107 46-47 0.39020689600210545 1107 48-49 0.4166083938373788 1107 50-51 0.3493271627569001 1107 52-53 0.30911441042191967 1107 54-55 0.38720644476441635 1107 56-57 0.3685707944473293 1107 58-59 0.22293348622096687 1107 60-61 0.13060883745835383 1107 62-63 0.29750503476120116 1107 64-65 0.1664385093795957 1107 66-67 0.19090607202057797 1107 68-69 0.1773344635415981 1107 70-71 0.20155513737629605 1107 72-73 0.36905374947294334 1107 74-75 0.2418122597909509 1107 76-77 0.03643893933522335 1107 78-79 0.22035889993239266 1107 80-81 0.45019197027343694 1107 82-83 0.4515012924004367 1107 84-85 0.4146565148641095 1107 86-87 0.5088952311528558 1107 88-89 0.31559376927581084 1107 90-91 0.3319763484298264 1107 92-93 0.19186664501889794 1107 94-95 -0.23518168067705858 1107 96-97 -0.0019223129566441344 1107 98-99 0.012053927002305898 1107 100-101 -0.3723475300602175 1108 1 0.1984862814376811 1108 2 0.14843581176722154 1108 3 0.15039805734221545 1108 4 0.1509224757047818 1108 5 0.12059688166206683 1108 6 0.07379423392229967 1108 7 0.13534514471773207 1108 8 0.12537352088629916 1108 9 0.2125390314545399 1108 10-11 0.1968096434193285 1108 12-13 0.18680736636011375 1108 14-15 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-0.5037292244110816 2316 74-75 -0.27902981445399533 2316 76-77 -0.33806718117338264 2316 78-79 -0.31357454194651524 2316 80-81 -0.592143969650099 2316 82-83 -0.3208085355784256 2316 84-85 -0.3519201383983912 2316 86-87 -0.11466773420177034 2316 88-89 0.06235826508851616 2316 90-91 0.09324255616625976 2316 92-93 -0.029708198191450208 2316 94-95 -0.06748538498187528 2316 96-97 0.15082536922697543 2316 98-99 0.26710207323840507 2316 100-101 0.461924498044219 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 21.0 4 63.0 5 124.0 6 242.0 7 679.0 8 1530.0 9 2574.0 10 4211.0 11 6564.0 12 9328.0 13 12388.0 14 16157.0 15 21138.0 16 26738.0 17 33896.0 18 32057.0 19 28055.0 20 27633.0 21 29002.0 22 31041.0 23 34816.0 24 40532.0 25 47309.0 26 56632.0 27 68397.0 28 82261.0 29 97816.0 30 116584.0 31 139944.0 32 168840.0 33 208755.0 34 270445.0 35 358929.0 36 472265.0 37 501423.0 38 218199.0 39 8964.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.43221860148157 18.165397168096558 14.825106470348906 23.577277760072956 2 16.79446601213742 19.87815951299092 37.46005810989714 25.867316364974513 3 19.901887182226076 22.845366187922135 29.15348250747592 28.09926412237587 4 14.029232685310127 16.118914646416137 34.34602770224417 35.50582496602956 5 14.734705892735125 36.806177147654985 32.9689087686299 15.490208190979986 6 34.72737836378208 34.43391785058045 15.985961509090538 14.852742276546934 7 30.766212520331095 31.89061439653856 18.834660397946184 18.508512685184165 8 28.484658587239082 33.210887545641626 18.647291575803283 19.657162291316006 9 27.281782239640002 15.975097266462083 18.71701167197545 38.02610882192246 10-11 26.27775302269997 25.67998349894743 25.825973727427176 22.216289750925426 12-13 27.22160378264587 22.66408549056779 26.29060117050405 23.82370955628229 14-15 23.494711947990194 24.146613741534946 24.455819533908247 27.902854776566617 16-17 23.498978918645715 27.43418079674262 25.554635331461746 23.512204953149922 18-19 23.772631870649384 25.585023720263074 27.63197299369716 23.01037141539038 20-21 23.875653232269634 24.696958484422407 28.085216599932927 23.34217168337503 22-23 23.919141693341793 24.558573225782578 28.167328230813197 23.35495685006243 24-25 23.859675696510582 24.97731097411971 27.913910183577993 23.249103145791715 26-27 22.90907579229714 25.408747868099045 27.82620663113905 23.85596970846476 28-29 23.3448011449967 25.86958815073271 27.61337781899542 23.172232885275175 30-31 23.139687393227327 25.290177622494337 27.44071508759886 24.129419896679476 32-33 22.813177539044354 25.242406445487482 27.511852258896475 24.432563756571685 34-35 24.00749749582191 25.395932562850103 27.21501854926322 23.381551392064768 36-37 24.169695797865757 24.608375297152968 27.312298046985834 23.90963085799544 38-39 23.335279536866544 25.105962594471965 27.334050720331497 24.224707148329998 40-41 23.857315547140825 24.933846594010898 27.617679055874966 23.591158802973307 42-43 23.52272092091351 24.94713895342292 27.267104754541943 24.263035371121628 44-45 23.458639513407892 25.237179012802486 27.626320438474533 23.677861035315086 46-47 24.235117955758913 25.707049633843532 26.53824292131612 23.519589489081437 48-49 23.701274265443363 25.814023060535874 26.391859060857076 24.092843613163684 50-51 23.952033581531417 25.912415310079655 26.627014805286002 23.50853630310292 52-53 24.516265660648294 25.2517906318738 26.62298401381806 23.60895969365985 54-55 23.617477889691724 25.876059460472263 26.693696062578038 23.812766587257975 56-57 23.618894964817176 26.02757628194127 26.506752362972776 23.846776390268786 58-59 23.570415250247596 25.334894215341887 26.49140071578039 24.603289818630127 60-61 22.916734240156444 25.844757845478977 27.006035165506503 24.232472748858076 62-63 23.338345580536316 25.860518869929823 27.128171296041163 23.672964253492694 64-65 22.96395433239229 25.97262525763213 26.763038272050082 24.300382137925496 66-67 22.836645196397225 25.976018672647356 26.82437458156907 24.362961549386345 68-69 23.71221708029112 25.28515359111429 27.07617998973713 23.92644933885746 70-71 23.469261953396124 25.57447108006279 26.38152774407847 24.574739222462618 72-73 23.27108174791493 25.814826069773627 26.330578434320927 24.58351374799051 74-75 23.21512302573881 26.258622508506384 26.248860435419953 24.277394030334857 76-77 23.4287864640984 25.67200064240739 26.61312746905659 24.286085424437616 78-79 23.440674149873015 25.95362070567192 26.63740668953931 23.968298454915754 80-81 23.736936602736524 25.937786320901107 26.24768797963548 24.077589096726886 82-83 23.504817526339217 26.015004374845795 26.345042678561338 24.135135420253654 84-85 23.18818444003696 26.20538916913302 26.395601066446307 24.210825324383716 86-87 23.662757558675985 26.245114927145103 25.840122311832193 24.252005202346723 88-89 23.29463453743933 26.442938564047623 25.65520125483735 24.607225643675694 90-91 23.096875393152256 26.294810303592662 25.95004086815262 24.65827343510246 92-93 23.17405898296566 25.754575539745055 26.2290952021507 24.842270275138585 94-95 22.91936960881132 25.449843050908942 26.566703993510423 25.064083346769316 96-97 23.28877944170389 25.0407409098567 26.45946928962024 25.21101035881917 98-99 22.829164819218832 25.61782959309122 26.028592824176712 25.524412763513244 100-101 22.93400681140777 24.982451886363172 26.57178036595178 25.511760936277277 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 467.0 1 328.0 2 292.5 3 428.0 4 403.5 5 392.5 6 695.0 7 1490.5 8 2320.0 9 2673.0 10 2358.0 11 1962.5 12 1945.0 13 2051.5 14 2332.5 15 2574.5 16 2714.5 17 3023.0 18 3389.0 19 3669.0 20 4186.5 21 4964.0 22 5830.5 23 6878.0 24 8228.0 25 9911.5 26 12332.0 27 15497.0 28 18434.5 29 21356.0 30 24733.0 31 31657.5 32 44360.0 33 46905.0 34 41414.5 35 42544.0 36 46818.0 37 52360.5 38 59470.5 39 64632.5 40 69230.5 41 76982.0 42 82287.5 43 87444.5 44 93717.5 45 101999.0 46 107439.0 47 112235.0 48 115629.0 49 114829.5 50 117524.5 51 119209.0 52 121982.0 53 130868.5 54 140274.0 55 135674.0 56 126704.5 57 120773.0 58 115495.5 59 104675.5 60 83284.0 61 67930.0 62 58556.0 63 44025.5 64 30689.5 65 23507.0 66 17902.5 67 14173.0 68 11040.5 69 8511.0 70 6640.0 71 4500.5 72 3271.5 73 2670.5 74 1939.5 75 1391.0 76 998.5 77 675.5 78 449.0 79 267.5 80 141.5 81 85.0 82 58.5 83 35.0 84 17.5 85 9.5 86 9.5 87 6.0 88 3.5 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.242828418969283E-4 2 0.04940868604070784 3 8.50245075270307E-4 4 0.0 5 0.08499301696868737 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.463961417767918E-4 26-27 0.008770120498621501 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011383836841119111 36-37 6.298111668668942E-5 38-39 0.004471659284754948 40-41 0.005369140197540272 42-43 3.9363197929180886E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.149055834334471E-5 68-69 9.289714711286689E-4 70-71 1.1021695420170648E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.919525878153582E-4 82-83 4.5661309597849823E-4 84-85 0.004692093193158361 86-87 0.002046886292317406 88-89 1.2596223337337883E-4 90-91 0.0031333105551627984 92-93 8.817356336136518E-4 94-95 9.447167503003412E-5 96-97 0.0 98-99 9.447167503003412E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3175555.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.50246760404553 #Duplication Level Percentage of deduplicated Percentage of total 1 68.79313297229359 25.799122406742363 2 15.045805954624717 11.285097007801374 3 5.794765858341219 6.519540566264119 4 2.9648915126373505 4.447629916087671 5 1.7332212712895798 3.250003728859004 6 1.128700908533595 2.539744155416274 7 0.7891924819724951 2.0717665841970847 8 0.548420721883353 1.6453704284654165 9 0.4237783836950444 1.4303461595236362 >10 2.4186265368698026 16.845106625740712 >50 0.21110934839747628 5.507207062855689 >100 0.13011056532592588 9.138808164453733 >500 0.011433704343289351 2.93476681911835 >1k 0.006053137593427079 4.763659826060866 >5k 7.566421991783849E-4 1.8218305484136832 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9144 0.287949665491544 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7716 0.24298114817724775 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6338 0.19958715878011876 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6334 0.19946119654674538 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6069 0.191116198585759 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5986 0.1885024822432614 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5643 0.1777012207314942 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5454 0.17174950520460203 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5099 0.16057035699271466 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4651 0.14646258685489624 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4448 0.14007000351119725 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4301 0.13544089143472557 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 4070 0.12816657245741297 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4028 0.12684396900699246 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3698 0.11645208475368873 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3624 0.11412178343628122 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3567 0.11232682161071057 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3341 0.10520995542511466 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3295 0.10376138974132082 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3275 0.10313157857445392 No Hit CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT 3178 0.10007699441514947 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 3.149055834334471E-5 14-15 0.0 0.0 0.0 0.0 7.872639585836176E-5 16-17 0.0 0.0 0.0 4.723583751501706E-5 9.447167503003412E-5 18-19 0.0 0.0 0.0 9.447167503003412E-5 9.447167503003412E-5 20-21 0.0 0.0 0.0 1.8894335006006824E-4 9.447167503003412E-5 22-23 0.0 0.0 0.0 5.195942126651876E-4 1.5745279171672352E-4 24-25 0.0 0.0 0.0 9.762073086436859E-4 1.5745279171672352E-4 26-27 0.0 0.0 0.0 0.001731980708883959 1.7319807088839588E-4 28-29 0.0 0.0 0.0 0.004739329030673378 1.8894335006006824E-4 30-31 0.0 0.0 0.0 0.01539888302989556 1.8894335006006824E-4 32-33 0.0 0.0 0.0 0.036985660774258355 1.8894335006006824E-4 34-35 0.0 0.0 0.0 0.06718510622552594 1.8894335006006824E-4 36-37 0.0 0.0 0.0 0.11761723541239247 1.8894335006006824E-4 38-39 0.0 0.0 0.0 0.19798114030460817 1.8894335006006824E-4 40-41 0.0 0.0 0.0 0.2822971102689136 1.8894335006006824E-4 42-43 0.0 0.0 0.0 0.37069110753868223 1.8894335006006824E-4 44-45 0.0 0.0 0.0 0.48405711757472314 2.046886292317406E-4 46-47 0.0 0.0 0.0 0.6135462934825566 2.2043390840341295E-4 48-49 0.0 0.0 0.0 0.7534273536436937 2.2043390840341295E-4 50-51 0.0 0.0 0.0 0.9106912020103572 2.2043390840341295E-4 52-53 0.0 0.0 0.0 1.0606649861205364 2.2043390840341295E-4 54-55 0.0 0.0 0.0 1.2174564761120497 2.2043390840341295E-4 56-57 0.0 0.0 0.0 1.4009047237412042 2.361791875750853E-4 58-59 0.0 0.0 0.0 1.600791042825585 2.5192446674675767E-4 60-61 0.0 0.0 0.0 1.824625931530079 2.5192446674675767E-4 62-63 3.149055834334471E-5 0.0 0.0 2.0356126724304886 2.5192446674675767E-4 64-65 3.149055834334471E-5 0.0 0.0 2.255117609362773 2.5192446674675767E-4 66-67 3.149055834334471E-5 0.0 0.0 2.5183786771131347 2.5192446674675767E-4 68-69 3.149055834334471E-5 0.0 0.0 2.8105480774226868 2.5192446674675767E-4 70-71 3.149055834334471E-5 0.0 0.0 3.113140852543886 2.6766974591843E-4 72-73 3.149055834334471E-5 0.0 0.0 3.4327857650080063 2.834150250901024E-4 74-75 3.149055834334471E-5 0.0 0.0 3.7537060450850324 2.834150250901024E-4 76-77 3.149055834334471E-5 0.0 0.0 4.0997085548825325 2.834150250901024E-4 78-79 3.149055834334471E-5 0.0 0.0 4.493545222803573 2.834150250901024E-4 80-81 3.149055834334471E-5 0.0 0.0 4.902843754871196 2.834150250901024E-4 82-83 3.149055834334471E-5 0.0 0.0 5.327572660527057 2.834150250901024E-4 84-85 3.149055834334471E-5 0.0 0.0 5.764992261195287 2.834150250901024E-4 86-87 3.149055834334471E-5 0.0 0.0 6.21308716114191 2.834150250901024E-4 88-89 3.149055834334471E-5 0.0 0.0 6.68637450776321 2.834150250901024E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5995 0.0 51.09254 1 GTATCAA 10025 0.0 42.082355 1 AGGTAAG 1945 0.0 33.012238 2 ATCAACG 13645 0.0 30.624142 3 TATCAAC 13695 0.0 30.56198 2 TAAGGTG 2385 0.0 30.495653 5 TCAACGC 13860 0.0 30.113844 4 CAACGCA 14165 0.0 29.465435 5 AAGGTAA 2170 0.0 29.37013 1 AACGCAG 14715 0.0 28.370928 6 GTAAGGT 2620 0.0 26.853153 4 TAAGGTA 1070 0.0 26.656456 4 GTGTAAG 1030 0.0 25.858995 1 TGGTATC 2800 0.0 24.970089 2 GGTAAGG 2755 0.0 24.847889 3 ACGCAGA 16805 0.0 24.729452 7 CGCAGAG 17100 0.0 24.247282 8 GTACATG 13850 0.0 24.24464 1 TACATGG 13500 0.0 24.168581 2 ACATGGG 13370 0.0 23.609417 3 >>END_MODULE