FastQCFastQC Report
Thu 26 May 2016
SRR938373_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938373_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3174206
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96430.30379250748061093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74450.23454684415567234No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA63230.19919942183966635No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA62400.19658459469864276No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA59570.187668979265996No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA55090.17355521349276007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52180.16438756652844838No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC51720.16293838522137505No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA50100.1578347467051603No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG47920.15096688746729103No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA47910.1509353835258329No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA43650.13751470446467556No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT39330.12390500175476953No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG39250.12365297022310462No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA34510.10872010197195772No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA34310.1080900231427954No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG32810.10336443192407803No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT32340.10188374667554657No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG32130.10122216390492614No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57000.054.061151
GTATCAA104200.044.382051
TATCAAC138950.033.1070062
ATCAACG139250.033.002073
TCAACGC143200.032.091754
CAACGCA144250.031.792265
AACGCAG149800.030.7383696
AGGTAAG19600.028.85322
TAAGGTG22800.027.0968275
ACGCAGA170750.026.6283827
AAGGTAA20600.026.3568521
CGCAGAG173150.026.2044128
TAAGGTA11600.024.1715184
GTAAGGT24800.023.9534594
GTACATG144450.023.8383711
TACATGG141550.023.7698522
GTGTAAG11250.023.7078081
TGGTATC24050.023.3168562
GCAGAGT193250.022.9850049
GAGTACT101150.022.35420812-13
Produced by FastQC (version 0.11.5)