FastQCFastQC Report
Thu 26 May 2016
SRR938373_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938373_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3174206
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89950.28337795341575184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77870.24532119213434792No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA63690.20064860314673968No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA63090.19875836665925276No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA61590.19403277544053538No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC58820.18530618365663729No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA56920.17932043477959528No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52810.16637231484030968No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG52120.16419854287969968No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA47290.14898213915542974No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA44750.1409801380250683No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA42650.134364310318864No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG39670.12497613576434548No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA39170.12340093869143968No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT36540.11511540208795522No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG36010.11344569319067509No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT35670.11237455918109915No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT34020.10717640884051002No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA33870.10670384971863829No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG32810.10336443192407803No Hit
CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT31850.1003400535440989No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57050.052.9436721
GTATCAA98300.041.3238071
TAAGGTG21000.034.863535
AGGTAAG18650.034.6861462
TATCAAC130650.030.8368472
ATCAACG131800.030.4797383
AAGGTAA20450.030.4701021
TCAACGC134050.029.9326764
CAACGCA137150.029.186785
GTAAGGT24750.028.8128324
AACGCAG142000.028.1623786
GTGTAAG9800.025.2390611
GGTAAGG27400.025.1586513
ACGCAGA163050.024.4391867
TAAGGTA9350.024.4061644
TACATGG131850.024.0986272
TGGTATC26850.024.0929832
CGCAGAG165500.024.0200028
GGACCGA13350.023.8363136
GTACATG135150.023.5102041
Produced by FastQC (version 0.11.5)