##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938373_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3174206 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.83607869180513 31.0 31.0 34.0 30.0 34.0 2 32.06574589046836 33.0 31.0 34.0 30.0 34.0 3 32.13051421363327 33.0 31.0 34.0 30.0 34.0 4 35.79830074040563 37.0 35.0 37.0 35.0 37.0 5 35.66268036794083 37.0 35.0 37.0 33.0 37.0 6 35.70448672833458 37.0 35.0 37.0 33.0 37.0 7 35.63868759620516 37.0 35.0 37.0 33.0 37.0 8 35.6164149396731 37.0 35.0 37.0 33.0 37.0 9 37.199733728686795 39.0 37.0 39.0 33.0 39.0 10-11 37.13110916556771 39.0 37.0 39.0 33.0 39.0 12-13 37.0229627503697 39.0 37.0 39.0 33.0 39.0 14-15 38.08686896817661 40.0 37.0 41.0 33.0 41.0 16-17 38.00639561515541 40.0 37.0 41.0 33.0 41.0 18-19 38.00120912127316 40.0 37.0 41.0 33.0 41.0 20-21 37.978736887271964 40.0 37.0 41.0 33.0 41.0 22-23 38.0113756007014 40.0 37.0 41.0 33.0 41.0 24-25 37.92186266423792 40.0 37.0 41.0 33.0 41.0 26-27 37.73630854456201 40.0 37.0 41.0 32.0 41.0 28-29 37.60818863047956 40.0 37.0 41.0 32.0 41.0 30-31 37.357140021788126 39.0 36.0 41.0 31.5 41.0 32-33 37.13134323985274 39.0 36.0 41.0 31.0 41.0 34-35 36.96451742577514 39.0 36.0 41.0 31.0 41.0 36-37 36.96680445440529 39.0 36.0 41.0 31.0 41.0 38-39 36.805101181208784 39.0 35.0 41.0 30.0 41.0 40-41 36.610064375153975 39.0 35.0 41.0 30.0 41.0 42-43 36.36375506189579 39.0 35.0 40.0 30.0 41.0 44-45 36.0707874662199 38.5 35.0 40.0 29.5 41.0 46-47 35.69469656348706 38.0 35.0 40.0 28.5 41.0 48-49 35.26415661113362 38.0 34.0 40.0 27.0 41.0 50-51 34.86345514437311 38.0 34.0 40.0 26.0 41.0 52-53 34.32680219872309 37.5 33.0 40.0 24.0 41.0 54-55 33.974779992224825 37.0 33.0 40.0 24.0 41.0 56-57 33.57266305337461 36.5 32.0 39.5 23.0 41.0 58-59 33.0848616945466 36.0 31.5 39.0 22.0 40.5 60-61 32.64989071282708 35.5 31.0 39.0 21.0 40.0 62-63 32.15223035303947 35.0 31.0 38.5 19.5 40.0 64-65 31.627193698203584 35.0 30.0 38.0 18.0 40.0 66-67 31.122102346224537 34.5 30.0 37.5 15.0 40.0 68-69 30.548140385343608 34.0 29.0 37.0 10.0 39.0 70-71 29.965667162118653 34.0 29.0 36.0 7.5 39.0 72-73 29.40086386957872 34.0 28.0 36.0 7.0 38.5 74-75 28.81328826799521 33.0 27.0 35.0 2.0 37.5 76-77 27.562146565156766 31.5 26.0 34.0 2.0 36.0 78-79 28.18858432628506 33.0 27.0 35.0 2.0 36.0 80-81 27.93335278176653 33.0 26.0 35.0 2.0 36.0 82-83 27.47662565063515 33.0 26.0 35.0 2.0 36.0 84-85 26.930637929611375 32.0 25.0 35.0 2.0 35.0 86-87 26.334451985787943 32.0 24.5 34.5 2.0 35.0 88-89 25.598053497473067 31.5 21.5 34.0 2.0 35.0 90-91 25.078387004498133 31.0 19.5 34.0 2.0 35.0 92-93 24.33607774668689 31.0 17.0 34.0 2.0 35.0 94-95 23.583179226553035 31.0 4.5 34.0 2.0 35.0 96-97 22.63035007809827 30.0 2.0 34.0 2.0 35.0 98-99 21.469922084451987 30.0 2.0 34.0 2.0 35.0 100-101 18.30879643602211 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32433251937886 1101 2 0.35847008178072315 1101 3 0.3377379781879952 1101 4 0.28884284258042214 1101 5 0.22900672792116694 1101 6 0.2721738621788745 1101 7 0.3378072347828507 1101 8 0.32759634812556016 1101 9 0.38659205949404907 1101 10-11 0.32143925668293605 1101 12-13 0.3843782260738067 1101 14-15 0.5482822812875696 1101 16-17 0.5538252731774378 1101 18-19 0.6400932349139339 1101 20-21 0.609785727736238 1101 22-23 0.48720157797062313 1101 24-25 0.5445329363632752 1101 26-27 0.542347589933307 1101 28-29 0.510420347877556 1101 30-31 0.6593479861753266 1101 32-33 0.6068168997697185 1101 34-35 0.5111823729270668 1101 36-37 0.6542773789043324 1101 38-39 0.7739862228439733 1101 40-41 0.8084340399675796 1101 42-43 0.7324893151343801 1101 44-45 0.9930101559742681 1101 46-47 0.9726772964591319 1101 48-49 0.8950931810544844 1101 50-51 1.0109624438998779 1101 52-53 1.0434528510455436 1101 54-55 1.0420102584718904 1101 56-57 1.0962960164403555 1101 58-59 1.2372622932392616 1101 60-61 1.1528434518870014 1101 62-63 1.3013805364954578 1101 64-65 1.3599111035402949 1101 66-67 1.4570896843591754 1101 68-69 1.5633845761633403 1101 70-71 1.614403058622102 1101 72-73 1.7815482647663963 1101 74-75 1.8813874154255252 1101 76-77 1.7086849322526412 1101 78-79 1.8985362046474528 1101 80-81 2.1342812806597067 1101 82-83 2.2098177981983547 1101 84-85 2.3614724973552974 1101 86-87 2.424175989126706 1101 88-89 2.580855998497121 1101 90-91 2.7645000109042073 1101 92-93 2.9476613073598408 1101 94-95 3.1423033993384983 1101 96-97 3.3945488593298094 1101 98-99 3.7689167744275345 1101 100-101 4.3570513291670885 1102 1 0.25714816288079234 1102 2 0.19293531519576845 1102 3 0.2530173303621268 1102 4 0.09307475336997584 1102 5 0.08036653176289832 1102 6 0.08465172418164713 1102 7 0.13968133958343998 1102 8 0.20972328446654842 1102 9 0.2821706549421066 1102 10-11 0.30327585740564444 1102 12-13 0.3630350735030987 1102 14-15 0.43634145150173254 1102 16-17 0.40687605780550484 1102 18-19 0.46701056657397544 1102 20-21 0.46647838464062374 1102 22-23 0.4631601501278766 1102 24-25 0.4032473013693263 1102 26-27 0.4386704467378735 1102 28-29 0.29758345689416643 1102 30-31 0.380789407723249 1102 32-33 0.29203281424823757 1102 34-35 0.46762965714123084 1102 36-37 0.5802341775238702 1102 38-39 0.57957189245937 1102 40-41 0.6164796813362656 1102 42-43 0.561552127845296 1102 44-45 0.4245336148823924 1102 46-47 0.4742478765158822 1102 48-49 0.5975578739152212 1102 50-51 0.6884763336977429 1102 52-53 0.7924246987571095 1102 54-55 0.6453732856559782 1102 56-57 0.8342736788576914 1102 58-59 0.9225655107364403 1102 60-61 0.9965722974538309 1102 62-63 1.1911087056200174 1102 64-65 1.100157020781527 1102 66-67 1.081843949467597 1102 68-69 1.113020151631364 1102 70-71 1.3678505911315462 1102 72-73 1.2111097163495685 1102 74-75 1.3890739528753961 1102 76-77 1.3741119127072565 1102 78-79 1.7279341160694806 1102 80-81 1.91003841889923 1102 82-83 1.9846134791594032 1102 84-85 2.2138760656132703 1102 86-87 2.1737549612641764 1102 88-89 2.2159715956872397 1102 90-91 2.388947653822619 1102 92-93 2.7146483015831038 1102 94-95 2.7426293056287605 1102 96-97 3.2465811087401697 1102 98-99 3.5978307098877487 1102 100-101 4.036371979291058 1103 1 0.27310479317035785 1103 2 0.2811714746052445 1103 3 0.3339240144023634 1103 4 0.2361199905336946 1103 5 0.14881150618773376 1103 6 0.22062313086438934 1103 7 0.22589713328904537 1103 8 0.24659925885318046 1103 9 0.2795193030848182 1103 10-11 0.46770356518324974 1103 12-13 0.42742988553708017 1103 14-15 0.6406493466462848 1103 16-17 0.5081124418977794 1103 18-19 0.48784455730812937 1103 20-21 0.48995506733560745 1103 22-23 0.4577187101270752 1103 24-25 0.4879769144902326 1103 26-27 0.4886682402437117 1103 28-29 0.4455498284995869 1103 30-31 0.5019149048072435 1103 32-33 0.6182757881658105 1103 34-35 0.5943977292181444 1103 36-37 0.7238966992240279 1103 38-39 0.7545768926598555 1103 40-41 0.7224801354470003 1103 42-43 0.8734674217206688 1103 44-45 0.904448586562161 1103 46-47 1.0356359250521123 1103 48-49 1.0849881528516718 1103 50-51 1.065101695426364 1103 52-53 1.2045467387629785 1103 54-55 1.0944328090389988 1103 56-57 1.2052024895264992 1103 58-59 1.4016083833925421 1103 60-61 1.3479007374316154 1103 62-63 1.5106482672299606 1103 64-65 1.3416450543468095 1103 66-67 1.386732864349355 1103 68-69 1.339179516028178 1103 70-71 1.4004187415508653 1103 72-73 1.2924747224435258 1103 74-75 1.5383466046139844 1103 76-77 1.3592305924360524 1103 78-79 1.5269327591941995 1103 80-81 1.8528053412266026 1103 82-83 2.074460557251026 1103 84-85 2.3346838749892562 1103 86-87 2.3866111825456464 1103 88-89 2.5730277267634314 1103 90-91 2.6299709000690292 1103 92-93 2.661807986040202 1103 94-95 2.6858193172804725 1103 96-97 3.1729533049207532 1103 98-99 3.3715606055822605 1103 100-101 3.8129466509682857 1104 1 0.2782508720880834 1104 2 0.28221969157264937 1104 3 0.23328167758094054 1104 4 0.18345845711339592 1104 5 0.10012097254826813 1104 6 0.20868176482560585 1104 7 0.22012676192058933 1104 8 0.23093769064990965 1104 9 0.3173477559393092 1104 10-11 0.34462401584900704 1104 12-13 0.38167592407729956 1104 14-15 0.5188247422069168 1104 16-17 0.49744071065016016 1104 18-19 0.5087477168965719 1104 20-21 0.48934642159433395 1104 22-23 0.435362095052902 1104 24-25 0.4284105106995426 1104 26-27 0.4388142780747515 1104 28-29 0.617337359242832 1104 30-31 0.5968562673564648 1104 32-33 0.5999184832856272 1104 34-35 0.5578048598626566 1104 36-37 0.6589407357058548 1104 38-39 0.6921498426285453 1104 40-41 0.8329040858539116 1104 42-43 0.7407087503761929 1104 44-45 0.8842964690276816 1104 46-47 0.9907323453664034 1104 48-49 0.9880139000186361 1104 50-51 0.9605555608783973 1104 52-53 1.1766999297292742 1104 54-55 1.193696490651206 1104 56-57 1.3964125396218563 1104 58-59 1.3535764749116126 1104 60-61 1.3944399048037894 1104 62-63 1.4949356502299977 1104 64-65 1.3891051262518808 1104 66-67 1.5716394855752753 1104 68-69 1.6433028629786541 1104 70-71 1.8104511189828116 1104 72-73 1.8202930522146978 1104 74-75 1.8706688115609644 1104 76-77 1.7349011074052818 1104 78-79 2.1477662177574928 1104 80-81 2.434530243482506 1104 82-83 2.4897021069866803 1104 84-85 2.6707306741846786 1104 86-87 2.7429038669660386 1104 88-89 2.810663308596453 1104 90-91 3.0817506682433304 1104 92-93 3.202260133168352 1104 94-95 3.4081455507223986 1104 96-97 3.630901591307765 1104 98-99 3.8353584536981877 1104 100-101 4.1754630110663875 1105 1 0.31020149853054946 1105 2 0.28150716850169033 1105 3 0.26598922701143124 1105 4 0.1842687024898524 1105 5 0.13103754829069914 1105 6 0.2085135125083113 1105 7 0.2800308538509384 1105 8 0.28172524745630767 1105 9 0.32496127951672804 1105 10-11 0.3070917993541613 1105 12-13 0.36856180311917797 1105 14-15 0.4718675811163706 1105 16-17 0.4775554430100115 1105 18-19 0.5115713073160464 1105 20-21 0.4081064168866675 1105 22-23 0.3055489528474027 1105 24-25 0.3172299317756071 1105 26-27 0.45427484967375875 1105 28-29 0.46399385429213424 1105 30-31 0.5161366339255906 1105 32-33 0.5456040541198561 1105 34-35 0.5424390895219631 1105 36-37 0.6482266533267946 1105 38-39 0.6542621081375017 1105 40-41 0.68422544013211 1105 42-43 0.6441247504067888 1105 44-45 0.6220563253843849 1105 46-47 0.6936773557504594 1105 48-49 0.896516983369807 1105 50-51 0.9993475984649436 1105 52-53 1.0641760939843223 1105 54-55 1.056823708522657 1105 56-57 0.9952191378319242 1105 58-59 1.2037955853157243 1105 60-61 1.2892166157089164 1105 62-63 1.3688047269836474 1105 64-65 1.5163749923468295 1105 66-67 1.4056130062295153 1105 68-69 1.471950411763327 1105 70-71 1.4311914886620478 1105 72-73 1.5791698490304817 1105 74-75 1.8852340556457925 1105 76-77 1.9470964899838599 1105 78-79 2.169014870889196 1105 80-81 2.3473952469155357 1105 82-83 2.341917354810448 1105 84-85 2.574379986219647 1105 86-87 2.720402721915544 1105 88-89 2.9187727516303 1105 90-91 2.9906652474765743 1105 92-93 3.2173875991694842 1105 94-95 3.269245190084604 1105 96-97 3.6564082814617755 1105 98-99 3.961312361485721 1105 100-101 4.0746983021598915 1106 1 0.19277087613722088 1106 2 0.2534009898802978 1106 3 0.1491227291893793 1106 4 0.11596063796839218 1106 5 0.03880824539901084 1106 6 0.08371567954044679 1106 7 0.1551859932759072 1106 8 0.08637897543291473 1106 9 0.19787625112238771 1106 10-11 0.2668248499843884 1106 12-13 0.165395718561264 1106 14-15 0.15024660254525202 1106 16-17 0.10359051547592912 1106 18-19 0.34091168672858174 1106 20-21 0.41571253804136177 1106 22-23 0.30139905074751283 1106 24-25 0.17657356281126368 1106 26-27 0.15550479214090984 1106 28-29 0.3613957391999705 1106 30-31 0.319151475631692 1106 32-33 0.30963517998764445 1106 34-35 0.3185426155796023 1106 36-37 0.3369240485554954 1106 38-39 0.2958081257363645 1106 40-41 0.278322100779242 1106 42-43 0.40317570435404804 1106 44-45 0.03482290473473881 1106 46-47 -0.20978065138699264 1106 48-49 0.18892467076087627 1106 50-51 0.0860395298091916 1106 52-53 0.25906056524105026 1106 54-55 0.31734977897435357 1106 56-57 0.3877952667425504 1106 58-59 0.8338040771579287 1106 60-61 0.6655903903038407 1106 62-63 0.8580810889031554 1106 64-65 0.5076636707449786 1106 66-67 0.6046769397963594 1106 68-69 0.39002459920896015 1106 70-71 0.603208966808161 1106 72-73 0.9878182569961105 1106 74-75 1.3192794903090466 1106 76-77 1.2503636787293146 1106 78-79 1.6281386814524872 1106 80-81 1.67753778294178 1106 82-83 1.9506802724155996 1106 84-85 1.7480165576983033 1106 86-87 2.105224721905625 1106 88-89 2.3672725606258638 1106 90-91 2.3026734213914857 1106 92-93 2.1010061058455456 1106 94-95 2.6285505788902555 1106 96-97 3.2579152527102337 1106 98-99 3.6998576249891535 1106 100-101 4.007858790949335 1107 1 0.1254675781630148 1107 2 0.07628345874024234 1107 3 0.11490116793437011 1107 4 0.027465447094456863 1107 5 -0.03428408382751513 1107 6 -0.03667838531458756 1107 7 0.01713132984594523 1107 8 0.042733914828154695 1107 9 0.1065145980623825 1107 10-11 0.0901064048229756 1107 12-13 0.14719866549899052 1107 14-15 0.21756655571418548 1107 16-17 0.20501598397083853 1107 18-19 0.19900652897342752 1107 20-21 0.2852776507636179 1107 22-23 0.18489694624820174 1107 24-25 0.1831017604489844 1107 26-27 0.2725721906535412 1107 28-29 0.2039389530433411 1107 30-31 0.2294729307159784 1107 32-33 0.30206743289505056 1107 34-35 0.3353113997159909 1107 36-37 0.2720653794283763 1107 38-39 0.3857977150114067 1107 40-41 0.45604039805825636 1107 42-43 0.5168153753574529 1107 44-45 0.5906637439600289 1107 46-47 0.5916593105664489 1107 48-49 0.4991909524419711 1107 50-51 0.4603257761066004 1107 52-53 0.5566189824767633 1107 54-55 0.39628940806955626 1107 56-57 0.5320957264554025 1107 58-59 0.5935958331804514 1107 60-61 0.666841445992624 1107 62-63 0.778770132487189 1107 64-65 0.9098332677924397 1107 66-67 1.0069593892989417 1107 68-69 1.0031342026728467 1107 70-71 1.0790943083207196 1107 72-73 1.1297032713004178 1107 74-75 1.1593077697488212 1107 76-77 1.113905211822793 1107 78-79 1.411123852433537 1107 80-81 1.5683574297828713 1107 82-83 1.4952865686687709 1107 84-85 1.6974747234356684 1107 86-87 1.986651231964327 1107 88-89 2.2779740391060237 1107 90-91 2.413052948570652 1107 92-93 2.57854817249482 1107 94-95 2.5528059958817977 1107 96-97 3.0319048989764994 1107 98-99 3.3266637432921335 1107 100-101 3.830593230010731 1108 1 0.13831710286007493 1108 2 0.17998200902713535 1108 3 0.10842925744952936 1108 4 0.11704994493457122 1108 5 0.07873911544979251 1108 6 0.045003274520816205 1108 7 0.07127009822981023 1108 8 0.03424522439526356 1108 9 0.12392202149960951 1108 10-11 0.17069136401497076 1108 12-13 0.22169681871017133 1108 14-15 0.2584128647610058 1108 16-17 0.32255274248121424 1108 18-19 0.3509829291211304 1108 20-21 0.30306543626538485 1108 22-23 0.18476111909768633 1108 24-25 0.15682588307465295 1108 26-27 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-0.0526004262798665 2316 56-57 -0.07599675641794335 2316 58-59 -0.15906802992920888 2316 60-61 0.018390409135228936 2316 62-63 0.07831525936055783 2316 64-65 0.09862589926019538 2316 66-67 0.2648097487072043 2316 68-69 0.371646537355776 2316 70-71 0.3642560767583589 2316 72-73 0.5213251723746666 2316 74-75 0.693761263400841 2316 76-77 0.702256352498484 2316 78-79 0.8198291452310187 2316 80-81 0.9995089375890238 2316 82-83 1.1110318111015438 2316 84-85 1.3865849118109672 2316 86-87 1.3682083970472085 2316 88-89 1.8027442353844734 2316 90-91 1.869314723867788 2316 92-93 1.9276166063029727 2316 94-95 2.3738886856637116 2316 96-97 2.7626402919268678 2316 98-99 3.0766734854633633 2316 100-101 3.4097177560004432 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 26.0 4 66.0 5 102.0 6 246.0 7 685.0 8 1538.0 9 2648.0 10 4186.0 11 6546.0 12 9360.0 13 12814.0 14 16664.0 15 22535.0 16 27922.0 17 34972.0 18 34333.0 19 30458.0 20 30572.0 21 32593.0 22 35278.0 23 39312.0 24 45083.0 25 52662.0 26 61887.0 27 74408.0 28 88467.0 29 104552.0 30 124043.0 31 146018.0 32 175647.0 33 214541.0 34 271369.0 35 346679.0 36 438625.0 37 460564.0 38 216005.0 39 10796.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.44716236033905 18.1531438580684 14.809408970120078 23.59028481147248 2 16.75917293408448 19.88216561111815 37.52807494299912 25.830586511798252 3 19.881904027411018 22.862593952866202 29.117087213841103 28.138414805881673 4 14.028988666772099 16.11965322981558 34.373698493418516 35.47765960999381 5 14.715626142205377 36.7581755493387 33.03518914999894 15.491009158456977 6 34.736875930547676 34.399720749062915 15.958195529842738 14.905207790546676 7 30.756730974612235 31.82691986594443 18.855014450857947 18.56133470858539 8 28.433976874846813 33.214069912286725 18.686877915295984 19.66507529757048 9 27.25472133818662 15.952808355853401 18.729660267796103 38.062810038163875 10-11 26.29501676954804 25.643672779901493 25.841423020434085 22.21988743011638 12-13 27.22661982240598 22.64490710432782 26.293252548826384 23.835220524439812 14-15 23.477981580275507 24.175683619777672 24.398463741798736 27.94787105814808 16-17 23.497718799599017 27.41625779801311 25.571150706664913 23.514872695722964 18-19 23.798896479938605 25.53419658333454 27.62560148900229 23.041305447724564 20-21 23.882350420861155 24.714180491121244 28.04049264603495 23.362976441982656 22-23 23.915429559392177 24.53167815825438 28.179141492392112 23.37375078996133 24-25 23.873517039426687 24.952267237312284 27.89174850532381 23.282467217937217 26-27 22.901559464939215 25.407211127197453 27.816103180842166 23.875126227021166 28-29 23.354155338374383 25.850748187105687 27.605407462527637 23.18968901199229 30-31 23.162296334894457 25.29624731350139 27.424842622060446 24.116613729543705 32-33 22.817233444075548 25.224037576848378 27.50457321611492 24.454155762961147 34-35 24.045325711199673 25.36043049531948 27.181783508066747 23.4124602854141 36-37 24.1754871417943 24.641461528561706 27.303065135125642 23.879986194518356 38-39 23.345310457907612 25.09907594236361 27.32859184555685 24.227021754171936 40-41 23.845589770046587 24.918852714033104 27.616101070805488 23.61945644511482 42-43 23.486597522400473 24.978150873965042 27.24179594460057 24.293455659033913 44-45 23.40816996490989 25.25711111825221 27.599985381747473 23.73473353509042 46-47 24.231437478045684 25.732600761450502 26.47644055755693 23.55952120294688 48-49 23.69616212684369 25.795411513934507 26.375399076178418 24.133027283043383 50-51 23.945531575455405 25.877179993989046 26.614797527318647 23.562490903236906 52-53 24.5097356630288 25.244722617246644 26.62048398875183 23.625057730972724 54-55 23.592309383827008 25.84520349340906 26.73038548852847 23.832101634235457 56-57 23.657632806440414 26.04852993157974 26.472352456015773 23.821484805964076 58-59 23.590812946607752 25.27817980307516 26.491191813007724 24.639815437309363 60-61 22.925449703012344 25.848290879671953 26.97852628342332 24.247733133892382 62-63 23.354171090345112 25.82915223523615 27.118718822911937 23.6979578515068 64-65 23.01550371967037 25.942629432368285 26.715106076921284 24.326760771040064 66-67 22.832778993745663 25.970044397048387 26.82536294606747 24.37181366313848 68-69 23.730652805328457 25.267197204461407 27.026191319672467 23.97595867053767 70-71 23.502696422349402 25.559903799564367 26.362970771273194 24.57442900681304 72-73 23.30002682561883 25.80345721266541 26.333905077634412 24.56261088408135 74-75 23.234125321418965 26.24963534187762 26.18761983311732 24.328619503586093 76-77 23.442917693432623 25.707673036973656 26.526791266855398 24.322618002738324 78-79 23.51088744712851 25.949985602698757 26.596273209741273 23.942853740431467 80-81 23.75705097811893 25.92269560409615 26.27390165411497 24.046351763669957 82-83 23.49538989647528 26.01352889314827 26.379750671789896 24.11133053858655 84-85 23.224994730725086 26.209893256619832 26.38290505443972 24.182206958215357 86-87 23.71524529430102 26.207797252592936 25.879442302230533 24.197515150875514 88-89 23.325793387635642 26.42735740130206 25.598913743756313 24.647935467305988 90-91 23.119522886974238 26.29393878628916 25.94756526460659 24.638973062130013 92-93 23.217996715079366 25.748507144237692 26.233258978675423 24.800237162007516 94-95 22.937657480327363 25.429382970103386 26.55816919254768 25.074790357021566 96-97 23.346121833302565 25.041490690900336 26.424497968940898 25.187889506856205 98-99 22.823628964219715 25.60418258928375 26.01089847350802 25.561289972988522 100-101 22.997374910508313 24.88167407147206 26.58458766089798 25.53636335712165 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 453.0 1 326.0 2 293.0 3 424.0 4 392.5 5 362.0 6 672.0 7 1516.5 8 2373.5 9 2749.0 10 2433.5 11 1959.0 12 1953.5 13 2130.5 14 2386.5 15 2614.0 16 2880.0 17 3128.0 18 3334.0 19 3710.0 20 4254.0 21 4939.0 22 5768.0 23 6822.5 24 8171.0 25 10037.5 26 12435.5 27 15396.0 28 18423.0 29 21385.0 30 24735.0 31 31731.5 32 44343.0 33 46708.0 34 41255.5 35 42531.5 36 46896.5 37 52620.0 38 59649.0 39 64742.0 40 68950.5 41 76364.5 42 81760.0 43 86909.5 44 93259.5 45 101394.0 46 106905.0 47 112043.0 48 115765.5 49 115073.5 50 116843.0 51 118020.5 52 121144.0 53 130220.5 54 139751.5 55 135384.0 56 126725.0 57 120493.0 58 115139.5 59 104980.5 60 83979.0 61 68773.5 62 59320.5 63 44628.0 64 31152.0 65 23913.0 66 18209.5 67 14272.5 68 11184.5 69 8727.5 70 6885.0 71 4698.5 72 3433.5 73 2759.5 74 2049.5 75 1471.5 76 1044.0 77 702.0 78 465.5 79 290.5 80 165.5 81 92.5 82 65.0 83 42.5 84 19.0 85 8.5 86 5.5 87 3.5 88 2.5 89 2.0 90 2.0 91 2.5 92 1.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.1503941458115825E-5 2 0.045617707231351716 3 2.520315316649266E-4 4 0.0 5 0.10260833732908324 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 7.403426242657219E-4 26-27 0.011372922866379812 28-29 0.0 30-31 0.0 32-33 5.513189755170269E-4 34-35 0.014696588690211034 36-37 0.003292161882373104 38-39 0.011908489871167782 40-41 0.006096012672145413 42-43 0.005229654282047227 44-45 0.002898362614146656 46-47 3.1503941458115825E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.677835023939845E-4 68-69 1.7327167801963703E-4 70-71 0.0 72-73 4.725591218717374E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.1503941458115825E-5 82-83 0.0011498938632212277 84-85 0.004568071511426795 86-87 0.0015594451021767335 88-89 3.1503941458115825E-5 90-91 9.923741559306485E-4 92-93 1.417677365615212E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 8.978623315563011E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3174206.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.415895564516646 #Duplication Level Percentage of deduplicated Percentage of total 1 68.76801472478392 25.730168571216588 2 14.924659710312383 11.16839018113995 3 5.944733743907021 6.672826107626495 4 2.992700735385115 4.478983126840866 5 1.738367173865269 3.2521282315063433 6 1.0989595681258921 2.467113385837482 7 0.7438234758439345 1.9481575043428698 8 0.5506685078530724 1.6483004304399018 9 0.4182322055927435 1.4083679273557992 >10 2.4627076749099484 17.08839242529449 >50 0.2068764432545465 5.389649038688569 >100 0.1319136501573294 9.21438612864735 >500 0.01149568892720057 2.949241895353975 >1k 0.0060859529613972955 4.7594841164629536 >5k 7.607441201746619E-4 1.8244109292463415 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8995 0.28337795341575184 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7787 0.24532119213434792 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6369 0.20064860314673968 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6309 0.19875836665925276 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6159 0.19403277544053538 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5882 0.18530618365663729 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5692 0.17932043477959528 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5281 0.16637231484030968 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5212 0.16419854287969968 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4729 0.14898213915542974 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4475 0.1409801380250683 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4265 0.134364310318864 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3967 0.12497613576434548 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3917 0.12340093869143968 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3654 0.11511540208795522 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3601 0.11344569319067509 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3567 0.11237455918109915 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3402 0.10717640884051002 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3387 0.10670384971863829 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3281 0.10336443192407803 No Hit CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT 3185 0.1003400535440989 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 3.1503941458115825E-5 6 0.0 0.0 0.0 0.0 3.1503941458115825E-5 7 0.0 0.0 0.0 0.0 3.1503941458115825E-5 8 0.0 0.0 0.0 0.0 3.1503941458115825E-5 9 0.0 0.0 0.0 0.0 3.1503941458115825E-5 10-11 0.0 0.0 0.0 0.0 3.1503941458115825E-5 12-13 0.0 0.0 0.0 0.0 4.725591218717374E-5 14-15 0.0 0.0 0.0 0.0 6.300788291623165E-5 16-17 0.0 0.0 0.0 3.1503941458115825E-5 6.300788291623165E-5 18-19 0.0 0.0 0.0 4.725591218717374E-5 9.451182437434748E-5 20-21 0.0 0.0 0.0 1.1026379510340538E-4 9.451182437434748E-5 22-23 0.0 0.0 0.0 3.4654335603927406E-4 9.451182437434748E-5 24-25 0.0 0.0 0.0 8.663583900981851E-4 9.451182437434748E-5 26-27 0.0 0.0 0.0 0.0019059884582160076 9.451182437434748E-5 28-29 0.0 0.0 0.0 0.004615327423613968 9.451182437434748E-5 30-31 0.0 0.0 0.0 0.014523317012191395 9.451182437434748E-5 32-33 0.0 0.0 0.0 0.03523715852090255 9.451182437434748E-5 34-35 0.0 0.0 0.0 0.06477210363788613 1.575197072905791E-4 36-37 0.0 0.0 0.0 0.1132881734833845 1.575197072905791E-4 38-39 0.0 0.0 0.0 0.19171723574336386 1.575197072905791E-4 40-41 0.0 0.0 0.0 0.2761635508218433 1.575197072905791E-4 42-43 0.0 0.0 0.0 0.36377601201686344 1.575197072905791E-4 44-45 0.0 0.0 0.0 0.4731576967594416 1.575197072905791E-4 46-47 0.0 0.0 0.0 0.6035997663667702 1.575197072905791E-4 48-49 0.0 0.0 0.0 0.7401536006169732 1.575197072905791E-4 50-51 0.0 0.0 0.0 0.8969487172540156 1.575197072905791E-4 52-53 0.0 0.0 0.0 1.0414730486931219 1.575197072905791E-4 54-55 0.0 0.0 0.0 1.1968662399352783 1.575197072905791E-4 56-57 0.0 0.0 0.0 1.3848187546743973 1.575197072905791E-4 58-59 0.0 0.0 0.0 1.5867275154794616 1.575197072905791E-4 60-61 0.0 0.0 0.0 1.806057955910864 1.575197072905791E-4 62-63 0.0 0.0 0.0 2.020158742060219 1.575197072905791E-4 64-65 0.0 0.0 0.0 2.243395671232428 1.575197072905791E-4 66-67 0.0 0.0 0.0 2.50821780312935 1.575197072905791E-4 68-69 0.0 0.0 0.0 2.799062190670675 1.575197072905791E-4 70-71 0.0 0.0 0.0 3.1022088673513943 1.575197072905791E-4 72-73 0.0 0.0 0.0 3.4189337428005615 1.575197072905791E-4 74-75 0.0 0.0 0.0 3.7347922598596313 1.575197072905791E-4 76-77 0.0 0.0 0.0 4.0807842969233885 1.575197072905791E-4 78-79 0.0 0.0 0.0 4.469779214077473 1.575197072905791E-4 80-81 0.0 0.0 0.0 4.8798817720084955 1.7327167801963703E-4 82-83 0.0 6.300788291623165E-5 0.0 5.309800309116674 2.362795609358687E-4 84-85 0.0 6.300788291623165E-5 0.0 5.747279792174799 2.8353547312304244E-4 86-87 0.0 6.300788291623165E-5 0.0 6.192115445563394 2.9928744385210033E-4 88-89 0.0 6.300788291623165E-5 0.0 6.662626811240354 3.150394145811582E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5705 0.0 52.943672 1 GTATCAA 9830 0.0 41.323807 1 TAAGGTG 2100 0.0 34.86353 5 AGGTAAG 1865 0.0 34.686146 2 TATCAAC 13065 0.0 30.836847 2 ATCAACG 13180 0.0 30.479738 3 AAGGTAA 2045 0.0 30.470102 1 TCAACGC 13405 0.0 29.932676 4 CAACGCA 13715 0.0 29.18678 5 GTAAGGT 2475 0.0 28.812832 4 AACGCAG 14200 0.0 28.162378 6 GTGTAAG 980 0.0 25.239061 1 GGTAAGG 2740 0.0 25.158651 3 ACGCAGA 16305 0.0 24.439186 7 TAAGGTA 935 0.0 24.406164 4 TACATGG 13185 0.0 24.098627 2 TGGTATC 2685 0.0 24.092983 2 CGCAGAG 16550 0.0 24.020002 8 GGACCGA 1335 0.0 23.836313 6 GTACATG 13515 0.0 23.510204 1 >>END_MODULE