FastQCFastQC Report
Thu 26 May 2016
SRR938372_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938372_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3182078
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89350.2807913570943264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71850.2257958478704796No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA63640.1999950975431778No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA63070.19820381524274389No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA59770.18783323350338993No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA55320.17384866115789746No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC51750.1626295772762327No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA48980.15392457381622954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47100.14801648482532484No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA46560.1463194805407033No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG46350.1456595344300171No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA43550.13686025295420162No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG40100.12601828113578611No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT38370.12058158222394298No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA35040.1101167224687767No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA34770.10926822032646592No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG33820.1062827498257428No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG32600.10244877718270891No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT32110.10090890292444119No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57850.049.5584071
GTATCAA105400.043.9465521
TATCAAC141250.032.6041832
ATCAACG141450.032.5580833
AGGTAAG19000.031.7678722
TCAACGC145200.031.619034
CAACGCA145550.031.4445425
AACGCAG151100.030.2509756
TAAGGTG24800.028.9371975
AAGGTAA20900.027.8458041
TAAGGTA10350.026.633344
ACGCAGA172050.026.31437
CGCAGAG173800.025.9946758
GTGTAAG11800.025.4685821
GTAAGGT26050.024.9948674
GGTAAGG26750.024.5184673
TACATGG137000.023.6592942
GTACATG140000.023.5448671
TGGTATC28900.023.352242
GCAGAGT191500.022.9700369
Produced by FastQC (version 0.11.5)