##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938372_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3182078 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.090771187884144 31.0 31.0 34.0 28.0 34.0 2 31.25466974725321 31.0 31.0 34.0 28.0 34.0 3 31.312874165875254 31.0 31.0 34.0 27.0 34.0 4 34.99329086213474 37.0 35.0 37.0 32.0 37.0 5 34.98564082967168 37.0 35.0 37.0 32.0 37.0 6 34.96020682082589 37.0 35.0 37.0 32.0 37.0 7 34.876837085703116 37.0 35.0 37.0 32.0 37.0 8 34.81965841189311 37.0 35.0 37.0 32.0 37.0 9 36.303508587784464 39.0 35.0 39.0 32.0 39.0 10-11 36.133755363633455 39.0 35.0 39.0 31.0 39.0 12-13 35.95427421955087 38.0 35.0 39.0 30.0 39.0 14-15 37.11087927448667 39.0 36.0 41.0 31.0 41.0 16-17 37.04367554786526 39.0 36.0 41.0 31.0 41.0 18-19 37.031219065026065 39.0 36.0 41.0 31.0 41.0 20-21 36.93996863056154 39.0 36.0 41.0 30.5 41.0 22-23 36.799221452145424 39.0 36.0 41.0 30.0 41.0 24-25 36.647913721788086 39.0 36.0 41.0 30.0 41.0 26-27 36.42535758080098 39.0 36.0 41.0 30.0 41.0 28-29 36.19177326891421 39.0 35.0 40.0 29.0 41.0 30-31 35.89552141713685 38.5 35.0 40.0 27.5 41.0 32-33 35.58614308008792 38.0 34.5 40.0 27.0 41.0 34-35 35.28132402788367 38.0 34.0 40.0 26.5 41.0 36-37 35.05428433872457 38.0 34.0 40.0 25.0 41.0 38-39 34.80154477671509 38.0 33.5 40.0 24.5 41.0 40-41 34.50230871147722 38.0 33.0 40.0 24.0 41.0 42-43 34.05647724537236 38.0 33.0 40.0 23.0 41.0 44-45 33.582298736863144 37.5 32.5 40.0 21.0 41.0 46-47 33.07448277509225 37.0 31.5 40.0 18.5 41.0 48-49 32.55374412569397 37.0 31.0 39.5 14.5 41.0 50-51 31.772631123435694 35.5 30.5 38.5 13.0 40.5 52-53 31.732697312887993 36.0 30.0 39.0 10.0 40.0 54-55 31.68418058891077 36.0 30.0 39.0 8.0 40.0 56-57 31.238365150068603 35.5 29.5 39.0 4.5 40.0 58-59 30.766912061866492 35.0 29.0 39.0 2.0 40.0 60-61 30.192165622590018 35.0 28.0 38.0 2.0 40.0 62-63 29.444879101015125 34.0 27.5 38.0 2.0 40.0 64-65 28.75649748371976 34.0 26.0 37.0 2.0 39.5 66-67 28.048863509945388 33.5 25.5 36.5 2.0 39.0 68-69 27.207415091647658 33.0 23.5 36.0 2.0 39.0 70-71 26.366733625008564 32.0 21.5 35.0 2.0 38.5 72-73 25.48426877028156 31.5 19.0 35.0 2.0 37.5 74-75 24.5736149459567 31.0 12.0 35.0 2.0 37.0 76-77 23.649519590657427 30.0 6.5 34.0 2.0 36.0 78-79 22.687749797459396 29.5 2.0 34.0 2.0 35.5 80-81 21.75563845386568 29.0 2.0 34.0 2.0 35.0 82-83 20.77423133562408 27.5 2.0 33.5 2.0 35.0 84-85 19.818760885182577 26.0 2.0 33.0 2.0 35.0 86-87 18.851645528487985 25.0 2.0 32.5 2.0 34.5 88-89 17.74826104199834 22.0 2.0 32.0 2.0 34.0 90-91 16.61373919809634 18.0 2.0 31.0 2.0 34.0 92-93 15.470317823761706 4.5 2.0 31.0 2.0 34.0 94-95 14.215701814977507 2.0 2.0 30.5 2.0 34.0 96-97 12.810185513994314 2.0 2.0 29.5 2.0 34.0 98-99 11.124018801550434 2.0 2.0 28.0 2.0 33.5 100-101 7.83351979429794 2.0 2.0 8.5 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6420838255483403 1101 2 0.4865661576883866 1101 3 0.5535165852438055 1101 4 0.4038909508949544 1101 5 0.5468500568503245 1101 6 0.49822793464236526 1101 7 0.47058604094479506 1101 8 0.45883475612671276 1101 9 0.5579817337257751 1101 10-11 0.715290459028374 1101 12-13 0.6344655649705899 1101 14-15 0.7386397437882337 1101 16-17 0.7979629478658339 1101 18-19 0.6926029777487557 1101 20-21 0.8167451974170348 1101 22-23 0.8618281367878922 1101 24-25 0.8473631158756518 1101 26-27 0.944048442856726 1101 28-29 0.9810073809505937 1101 30-31 1.0210285818308975 1101 32-33 1.108057948787092 1101 34-35 1.2037659505947502 1101 36-37 1.2584624399843634 1101 38-39 1.3958946429827606 1101 40-41 1.3229602537102565 1101 42-43 1.3520930835798168 1101 44-45 1.3678005155157535 1101 46-47 1.299410097559722 1101 48-49 1.3288192751142134 1101 50-51 1.5651158293328322 1101 52-53 1.521268710266682 1101 54-55 1.6880640087490342 1101 56-57 1.9141415537806097 1101 58-59 1.8478188759786356 1101 60-61 1.9750540202208562 1101 62-63 1.8889030064260801 1101 64-65 1.9328167856453788 1101 66-67 2.1015144114296582 1101 68-69 2.1390706286594856 1101 70-71 2.375400792171714 1101 72-73 2.5939366973060594 1101 74-75 2.6920435333719652 1101 76-77 2.880791372076633 1101 78-79 3.0341757207898397 1101 80-81 3.2492114169783157 1101 82-83 3.3964910897808878 1101 84-85 3.568086411969812 1101 86-87 3.708348365186577 1101 88-89 3.896972473543041 1101 90-91 3.9476181392667407 1101 92-93 3.7375070726610975 1101 94-95 3.4173117603286762 1101 96-97 3.2246602203823507 1101 98-99 2.704695031129148 1101 100-101 2.1897562709978233 1102 1 0.5819727807685275 1102 2 0.38412212085492925 1102 3 0.34311895832333406 1102 4 0.32019164407420675 1102 5 0.2667620871138965 1102 6 0.38536610342423927 1102 7 0.39182702048966433 1102 8 0.38182152045504125 1102 9 0.46133219290676664 1102 10-11 0.5256887045827199 1102 12-13 0.5274805080717329 1102 14-15 0.5312209626825606 1102 16-17 0.6373709771148413 1102 18-19 0.6345668309716785 1102 20-21 0.6057614149039665 1102 22-23 0.56040051540419 1102 24-25 0.6887729824672562 1102 26-27 0.6252926392977258 1102 28-29 0.6629915872278715 1102 30-31 0.41280205048007446 1102 32-33 0.554865272174851 1102 34-35 0.7554538003162108 1102 36-37 0.882398683577641 1102 38-39 1.1282063230995547 1102 40-41 1.1356761491982468 1102 42-43 1.1883995300540704 1102 44-45 1.0710745734137035 1102 46-47 0.9962048936115977 1102 48-49 1.0266136597745685 1102 50-51 1.047094653495435 1102 52-53 1.1515936477073367 1102 54-55 1.2137894033601135 1102 56-57 1.2866221209441449 1102 58-59 1.4430581171623267 1102 60-61 1.470582905782873 1102 62-63 1.536667294548618 1102 64-65 1.743004324503321 1102 66-67 1.8685331220110832 1102 68-69 1.9439922322822056 1102 70-71 1.6971681054138834 1102 72-73 1.915341862280247 1102 74-75 1.69943466154362 1102 76-77 1.9059304255049447 1102 78-79 2.3548778083698387 1102 80-81 2.4947102678659014 1102 82-83 2.8742717407693092 1102 84-85 2.784748511056403 1102 86-87 2.9448225233032126 1102 88-89 3.092345525232993 1102 90-91 3.0353636209196075 1102 92-93 3.1196591761709147 1102 94-95 3.1193592528509413 1102 96-97 2.923887484814305 1102 98-99 2.758451592514639 1102 100-101 2.2398809566544875 1103 1 0.4236050169659755 1103 2 0.33616121795563814 1103 3 0.23784925209577423 1103 4 0.1733469765739173 1103 5 0.19425606952841434 1103 6 0.23148164885635225 1103 7 0.31207334044000135 1103 8 0.2683441003150193 1103 9 0.41375540502195207 1103 10-11 0.47033488613833185 1103 12-13 0.4099031264258457 1103 14-15 0.5406602881510949 1103 16-17 0.5016320025083374 1103 18-19 0.5258834707656419 1103 20-21 0.5900486436935637 1103 22-23 0.6566207953312571 1103 24-25 0.573217870756956 1103 26-27 0.7068856211599126 1103 28-29 0.5729182099278844 1103 30-31 0.5488672395434051 1103 32-33 0.6183486004145919 1103 34-35 0.7024294611882098 1103 36-37 0.6598761575457033 1103 38-39 0.8029600627614215 1103 40-41 0.82061967874305 1103 42-43 0.9301775623345989 1103 44-45 1.0441403672161798 1103 46-47 1.2510599527569255 1103 48-49 1.0300369933569726 1103 50-51 1.0148140597201945 1103 52-53 1.1626334130548486 1103 54-55 1.1515490188956967 1103 56-57 1.5032508896005439 1103 58-59 1.453800411754937 1103 60-61 1.5434834852724286 1103 62-63 1.7403842388487512 1103 64-65 1.5626221312443604 1103 66-67 1.9002677625159023 1103 68-69 1.9678238303104472 1103 70-71 1.950848037033385 1103 72-73 2.31985009059283 1103 74-75 2.294334974017765 1103 76-77 2.3698669952964835 1103 78-79 2.6817975777922314 1103 80-81 2.7047127574128105 1103 82-83 2.6237467107890957 1103 84-85 2.8421338068645063 1103 86-87 2.85853363505176 1103 88-89 3.1284902877232525 1103 90-91 3.137807413997532 1103 92-93 3.151930267408149 1103 94-95 3.082926522143776 1103 96-97 3.152298515265983 1103 98-99 2.8007254920174525 1103 100-101 2.1830704168406827 1104 1 0.5675318939872298 1104 2 0.4731444154552271 1104 3 0.3973921309376287 1104 4 0.3870377876765261 1104 5 0.3379686027839526 1104 6 0.47808018199081914 1104 7 0.49894934951785785 1104 8 0.5037825342030331 1104 9 0.5154271210058781 1104 10-11 0.5153480692722567 1104 12-13 0.536685148408651 1104 14-15 0.5201431573540916 1104 16-17 0.6976894003287128 1104 18-19 0.6694969361850127 1104 20-21 0.5813763213946288 1104 22-23 0.6773005032283308 1104 24-25 0.6579076395799248 1104 26-27 0.8460094447086846 1104 28-29 0.9543207789437673 1104 30-31 0.6794118546359158 1104 32-33 0.8079268955943988 1104 34-35 0.8198938141330103 1104 36-37 0.9451749868251937 1104 38-39 0.9990814660118374 1104 40-41 1.0376996352524444 1104 42-43 0.9284444540622161 1104 44-45 0.8528638607813974 1104 46-47 0.948393655237858 1104 48-49 1.1898737679331646 1104 50-51 1.1148950671094369 1104 52-53 1.1744735906690948 1104 54-55 1.6451039863290013 1104 56-57 1.7888533725481466 1104 58-59 1.8309791185541648 1104 60-61 1.9823048790295381 1104 62-63 2.0769663025756664 1104 64-65 2.1157645001597523 1104 66-67 2.254286261573686 1104 68-69 2.5328894019715555 1104 70-71 2.429987393889622 1104 72-73 2.551536020082331 1104 74-75 2.5005804816469777 1104 76-77 2.5384040428632666 1104 78-79 2.7772999641859 1104 80-81 2.9083834936340303 1104 82-83 3.057430304328488 1104 84-85 3.181103091204413 1104 86-87 3.274981575151937 1104 88-89 3.4267365720178447 1104 90-91 3.2876485912211244 1104 92-93 3.4147230012868004 1104 94-95 3.562855113915159 1104 96-97 3.413458697206792 1104 98-99 3.1719186160811432 1104 100-101 2.166529230586865 1105 1 0.5383976066181049 1105 2 0.4006664526736401 1105 3 0.43538705606726325 1105 4 0.4053622214686996 1105 5 0.3555849947459464 1105 6 0.4298642451052004 1105 7 0.33800723995157256 1105 8 0.5279673757588341 1105 9 0.42996195930935244 1105 10-11 0.47702915665021806 1105 12-13 0.5747161758909485 1105 14-15 0.6650520876133754 1105 16-17 0.6967989793721685 1105 18-19 0.746981610580697 1105 20-21 0.7250518583471006 1105 22-23 0.680510967184027 1105 24-25 0.7156513296823093 1105 26-27 0.6398324877822219 1105 28-29 0.6598416689787996 1105 30-31 0.7516112690930967 1105 32-33 0.9708697290751047 1105 34-35 0.869929651942563 1105 36-37 0.9395632495768709 1105 38-39 0.932033795033739 1105 40-41 1.012929686601808 1105 42-43 1.1790501799575992 1105 44-45 1.1287068429353226 1105 46-47 1.3150037096808305 1105 48-49 1.288882602739065 1105 50-51 1.1223673204284381 1105 52-53 1.2077232350142282 1105 54-55 1.3554131064991033 1105 56-57 1.4561810857410933 1105 58-59 1.5044882703449467 1105 60-61 1.6873816478814234 1105 62-63 1.6820275723140874 1105 64-65 1.7456260631986602 1105 66-67 1.923821820368076 1105 68-69 2.259393228354096 1105 70-71 2.2640916369431103 1105 72-73 2.378669396800735 1105 74-75 2.6216238894351918 1105 76-77 2.7590630228410866 1105 78-79 2.9778542909128127 1105 80-81 2.902822019574973 1105 82-83 3.1431687234873813 1105 84-85 3.3832121532995245 1105 86-87 3.6245289107827467 1105 88-89 3.5212430388517646 1105 90-91 3.3148269713894827 1105 92-93 3.1474527717536436 1105 94-95 3.216305829690988 1105 96-97 3.367366885811709 1105 98-99 3.0995049217118353 1105 100-101 2.1188330430886753 1106 1 0.3502504643862103 1106 2 0.32550921996923776 1106 3 0.133064089000694 1106 4 0.26029714001946047 1106 5 0.261745698860679 1106 6 -0.027756369915991286 1106 7 0.1836538875524667 1106 8 0.038107311366871954 1106 9 0.2926463967190429 1106 10-11 0.27326591402921707 1106 12-13 0.4675551180401101 1106 14-15 0.5738954904348716 1106 16-17 -0.004312891172411071 1106 18-19 0.4934965604281061 1106 20-21 0.45280185684821816 1106 22-23 0.37158992228352616 1106 24-25 0.5998276341591691 1106 26-27 0.5367639768913435 1106 28-29 0.5762962438659329 1106 30-31 0.418610537638763 1106 32-33 0.5661475018566051 1106 34-35 0.48986852754136834 1106 36-37 0.5867771280235488 1106 38-39 0.6683878249274429 1106 40-41 0.8357219835482894 1106 42-43 0.46842190864799704 1106 44-45 0.7767917216158224 1106 46-47 0.886299672892271 1106 48-49 0.5486074979672253 1106 50-51 0.5475555799868133 1106 52-53 0.7550534266912408 1106 54-55 0.6469836948402055 1106 56-57 1.1444590803571089 1106 58-59 1.268055699043142 1106 60-61 1.4680998820787075 1106 62-63 1.4400782165335002 1106 64-65 1.285629720470336 1106 66-67 1.5199882801890503 1106 68-69 1.6496458498984232 1106 70-71 1.3384736479628891 1106 72-73 1.7731449680245994 1106 74-75 2.1728291403180293 1106 76-77 2.189444458290243 1106 78-79 2.581355828765574 1106 80-81 2.418355681463204 1106 82-83 2.62474761563384 1106 84-85 2.462857937544481 1106 86-87 2.1316638880896797 1106 88-89 2.1226548356513533 1106 90-91 2.187875277353232 1106 92-93 2.581306816636163 1106 94-95 2.6384162852911075 1106 96-97 2.6065406122118144 1106 98-99 2.5238610020758827 1106 100-101 1.8784126966671062 1107 1 0.35111197450255816 1107 2 0.2912631977007649 1107 3 0.27518482552076406 1107 4 0.2923561207648504 1107 5 0.23378898476831012 1107 6 0.34017953145673374 1107 7 0.3531837248038201 1107 8 0.2998443204767014 1107 9 0.34020857898387646 1107 10-11 0.34331976551965226 1107 12-13 0.4034735503760203 1107 14-15 0.43940835906452946 1107 16-17 0.44070172322363277 1107 18-19 0.5201143797555048 1107 20-21 0.5950300688877519 1107 22-23 0.6252605808980505 1107 24-25 0.5399649071242436 1107 26-27 0.5814265947447907 1107 28-29 0.6028537859178655 1107 30-31 0.7128224975600617 1107 32-33 0.8132322994728085 1107 34-35 0.6643874466447173 1107 36-37 0.6082022875308652 1107 38-39 0.8613595514733206 1107 40-41 0.8933157829500615 1107 42-43 0.9888485125353128 1107 44-45 0.8225219864946425 1107 46-47 0.7512908018970208 1107 48-49 0.6402781452086828 1107 50-51 0.9731981584894314 1107 52-53 1.1309675372602328 1107 54-55 1.2373404680725493 1107 56-57 1.3717865793051018 1107 58-59 1.3979819446787296 1107 60-61 1.5171527896042711 1107 62-63 1.5740424709718397 1107 64-65 1.538123266252633 1107 66-67 1.7246364542425248 1107 68-69 1.8876657413993279 1107 70-71 1.8111896510108707 1107 72-73 1.6317967473415358 1107 74-75 1.8670610031133066 1107 76-77 1.9000964764416644 1107 78-79 2.1939534950114385 1107 80-81 2.344164875453675 1107 82-83 2.3334528483837325 1107 84-85 2.4760006582104843 1107 86-87 2.5210653233712215 1107 88-89 2.7199909781331826 1107 90-91 2.5309274683753316 1107 92-93 2.7481537464756887 1107 94-95 2.7854806113797963 1107 96-97 2.6892236902197073 1107 98-99 2.5491811540201113 1107 100-101 1.7547009812668302 1108 1 0.20785659646168142 1108 2 0.20098091808244334 1108 3 0.14190842862333497 1108 4 0.2615550016546848 1108 5 0.26988249131350983 1108 6 0.31894174329155334 1108 7 0.1819832900681888 1108 8 0.2534178459580687 1108 9 0.27662831495803886 1108 10-11 0.33959846119902437 1108 12-13 0.2530307469709214 1108 14-15 0.26489391810782337 1108 16-17 0.4306222503370307 1108 18-19 0.3067237616859657 1108 20-21 0.35744022162809586 1108 22-23 0.3842176738558507 1108 24-25 0.4331707945365295 1108 26-27 0.41268771902970514 1108 28-29 0.4445155834885739 1108 30-31 0.45918640241864495 1108 32-33 0.5608802062591209 1108 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0.1324233246537645 2316 96-97 0.3468533798621678 2316 98-99 0.3374447167713761 2316 100-101 0.46139532885153844 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10139.0 3 3885.0 4 4310.0 5 5055.0 6 6059.0 7 7864.0 8 10110.0 9 13300.0 10 17054.0 11 21349.0 12 26293.0 13 30855.0 14 35002.0 15 40388.0 16 46868.0 17 54133.0 18 54405.0 19 54336.0 20 58782.0 21 64446.0 22 71930.0 23 79315.0 24 88531.0 25 97603.0 26 107367.0 27 119888.0 28 132274.0 29 147180.0 30 163051.0 31 182330.0 32 204229.0 33 229879.0 34 259100.0 35 276682.0 36 258759.0 37 163624.0 38 35256.0 39 447.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.61631898433852 17.373042315021646 14.364927624023524 23.645711076616312 2 16.33627869590926 19.246570196585584 38.704226981424064 25.712924126081095 3 19.76827932228539 22.837988861940563 28.753546464381312 28.640185351392727 4 13.496677140271904 15.505263945541445 34.72845863198409 36.26960028220256 5 14.682627281469196 36.70825055234494 32.915733513454704 15.69338865273116 6 34.52632796955298 34.12693721182363 16.35904795204544 14.987686866577954 7 30.68294636665038 31.856735873625464 18.958663822183322 18.50165393754083 8 28.330509670821417 33.06860383852528 18.82684446361468 19.774042027038625 9 27.3032815256172 16.198884036707515 18.797118703372504 37.700715734302776 10-11 26.284810045998153 25.57926818245908 25.857225111788733 22.278696659754026 12-13 27.345182761640647 22.686991259102875 26.232420599169227 23.73540538008725 14-15 23.621485382624368 24.139636629286805 24.467530320185944 27.771347667902884 16-17 23.548998319583422 27.37435066955768 25.515912407107162 23.560738603751734 18-19 23.830658235738543 25.55494764828689 27.558767876573278 23.055626239401285 20-21 23.9931184837401 24.701452537500177 28.011229226167366 23.294199752592355 22-23 23.94541621581519 24.518691815010165 28.063814791365534 23.472077177809116 24-25 23.755056806794023 24.833313028818 27.952935443430967 23.45869472095701 26-27 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26.44016571599818 23.689410893549802 52-53 24.470232643801854 25.224638833556373 26.587746332706217 23.717382189935552 54-55 23.598581562958465 25.85471737487947 26.67775468820911 23.868946373952955 56-57 23.606612197267165 26.058206608142942 26.438606110120837 23.896575084469056 58-59 23.58044327618588 25.206980386832704 26.49080247798201 24.721773858999406 60-61 22.93143305310297 25.753929361457256 27.00306252988634 24.311575055553433 62-63 23.401921167573043 25.70921746192536 27.16444076787615 23.724420602625443 64-65 23.019826251586345 25.882727677867095 26.765486784070358 24.3319592864762 66-67 22.936009465878683 25.83432198129766 26.799656186212562 24.43001236661109 68-69 23.762192492730705 25.21224900027531 27.090543440410624 23.93501506658336 70-71 23.588128884475392 25.482001429758817 26.32175293648228 24.60811674928351 72-73 23.40629890226352 25.676291098330502 26.33733105490757 24.580078944498414 74-75 23.429520064757618 26.20815124443283 26.241545231723627 24.120783459085924 76-77 23.779136222397447 25.603356729156445 26.354698613127287 24.26280843531882 78-79 23.799154532378715 25.923949400172454 26.318522956971226 23.95837311047761 80-81 24.16122464501927 25.898004288243914 25.908784614117764 24.03198645261905 82-83 23.887897706405656 25.965363109352634 25.98052812277092 24.166211061470793 84-85 23.743460096492157 26.100119648766285 26.021249520320648 24.13517073442091 86-87 24.294098069983182 26.095893359916257 25.457079886725083 24.15292868337548 88-89 23.9612831136951 26.22691873398485 25.25986093289215 24.551937219427906 90-91 23.82656404296842 26.17089765978424 25.422217849400546 24.580320447846795 92-93 24.09645673158141 25.55837808744436 25.66963805153631 24.675527129437924 94-95 23.887771374794845 25.321268057797525 25.93600995028472 24.854950617122906 96-97 24.294535856975056 24.94416918881014 25.764980337810588 24.996314616404216 98-99 23.876248935320945 25.368025370005437 25.277036562100253 25.47868913257336 100-101 25.040735361975596 24.0536629102189 25.538558692667447 25.367043035138053 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 416.0 1 326.0 2 307.5 3 419.5 4 388.5 5 364.5 6 605.5 7 1291.5 8 2075.0 9 2377.5 10 2121.0 11 1785.0 12 1826.0 13 1966.5 14 2165.5 15 2422.5 16 2719.5 17 3017.5 18 3338.0 19 3757.0 20 4289.5 21 5004.0 22 6066.0 23 7347.0 24 8646.5 25 10377.0 26 12845.5 27 15969.5 28 18839.0 29 21679.0 30 25381.5 31 32603.5 32 42798.5 33 45130.0 34 41668.5 35 42551.5 36 46657.0 37 52475.0 38 59027.5 39 64216.5 40 69092.0 41 75909.5 42 81435.5 43 86408.0 44 92010.5 45 99223.0 46 105292.0 47 110492.5 48 114331.0 49 114523.5 50 115772.5 51 117122.0 52 120176.5 53 127242.5 54 134035.0 55 131699.0 56 125532.5 57 120687.0 58 115871.5 59 105507.0 60 86817.0 61 71966.0 62 61440.0 63 48395.0 64 36172.5 65 27994.0 66 21506.5 67 16592.0 68 12887.0 69 9922.0 70 7792.5 71 5731.0 72 4177.5 73 3239.5 74 2445.5 75 1793.0 76 1292.5 77 876.5 78 581.0 79 383.5 80 244.5 81 152.5 82 92.5 83 49.5 84 30.0 85 22.5 86 18.0 87 13.0 88 8.0 89 7.0 90 6.0 91 3.5 92 1.5 93 1.5 94 2.0 95 2.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3933907339794939 2 0.028880498843837265 3 0.03434862376095118 4 0.04349359129474513 5 0.0628520105415392 6 0.0544926931395145 7 0.03720839024059121 8 0.038371152435609686 9 0.03610848005611427 10-11 0.03738123326958045 12-13 0.029540444954523428 14-15 0.02715206855394494 16-17 0.022752427816037195 18-19 0.02685352150387263 20-21 0.03434862376095118 22-23 0.033311565587015785 24-25 0.04212656006546665 26-27 0.03617133206665581 28-29 0.03164598730766499 30-31 0.03453717979257579 32-33 0.02872336881748342 34-35 0.03076605916008344 36-37 0.03280874950268346 38-39 0.015351603574770951 40-41 0.01272753213466169 42-43 0.013481756261160162 44-45 0.011313361897477057 46-47 0.019971226349574083 48-49 0.017237163891017128 50-51 0.011611908947549368 52-53 0.00955350560231396 54-55 0.008359317402024714 56-57 0.00916068053642934 58-59 0.004211084706283127 60-61 0.007275120220183164 62-63 0.011533343934372445 64-65 0.007416537243901626 66-67 0.0038653986483046616 68-69 0.006348053064695459 70-71 0.013277487226900158 72-73 0.010967675839498592 74-75 0.01154905693700783 76-77 0.005373846901301603 78-79 0.00906640252061703 80-81 0.01118765787639398 82-83 0.012963227174192462 84-85 0.008327891396753946 86-87 0.0026712104480154162 88-89 0.016357235743435578 90-91 0.025502203277229532 92-93 0.01070055479469705 94-95 0.020788302486614094 96-97 0.018572769115024836 98-99 0.011564769939643215 100-101 0.12389702578000916 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3182078.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.59692401902969 #Duplication Level Percentage of deduplicated Percentage of total 1 71.91134711750352 29.193794950353603 2 13.584428747913591 11.029720434419414 3 5.288122400083225 6.440445098385229 4 2.7055246716521966 4.393439181066979 5 1.5469242081451169 3.1400182270632495 6 0.9805886286885671 2.388532923167657 7 0.6917401667602638 1.965776609361415 8 0.49072813431578166 1.593764222625439 9 0.3541135294523968 1.2938328044360448 >10 2.1373613573446546 16.052454065023053 >50 0.18251664577355256 5.1366282627948605 >100 0.11087214901344816 8.475279639029687 >500 0.009470653959211286 2.5464929862291443 >1k 0.005713700322377271 4.904845258241675 >5k 5.478890720087794E-4 1.444975337802555 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8935 0.2807913570943264 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7185 0.2257958478704796 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6364 0.1999950975431778 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6307 0.19820381524274389 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5977 0.18783323350338993 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5532 0.17384866115789746 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5175 0.1626295772762327 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4898 0.15392457381622954 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4710 0.14801648482532484 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4656 0.1463194805407033 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4635 0.1456595344300171 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4355 0.13686025295420162 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4010 0.12601828113578611 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3837 0.12058158222394298 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3504 0.1101167224687767 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3477 0.10926822032646592 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3382 0.1062827498257428 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3260 0.10244877718270891 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3211 0.10090890292444119 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.285201054153921E-5 2 0.0 0.0 0.0 0.0 6.285201054153921E-5 3 0.0 0.0 0.0 0.0 6.285201054153921E-5 4 0.0 0.0 0.0 0.0 6.285201054153921E-5 5 0.0 0.0 0.0 0.0 6.285201054153921E-5 6 0.0 0.0 0.0 0.0 9.427801581230881E-5 7 0.0 0.0 0.0 0.0 9.427801581230881E-5 8 0.0 0.0 0.0 0.0 9.427801581230881E-5 9 0.0 0.0 0.0 0.0 9.427801581230881E-5 10-11 0.0 0.0 0.0 0.0 9.427801581230881E-5 12-13 0.0 0.0 0.0 0.0 1.57130026353848E-4 14-15 0.0 3.142600527076961E-5 0.0 0.0 1.57130026353848E-4 16-17 0.0 3.142600527076961E-5 0.0 0.0 1.57130026353848E-4 18-19 0.0 3.142600527076961E-5 0.0 0.0 1.57130026353848E-4 20-21 0.0 3.142600527076961E-5 0.0 0.0 1.57130026353848E-4 22-23 0.0 3.142600527076961E-5 0.0 2.5140804216615685E-4 1.57130026353848E-4 24-25 0.0 3.142600527076961E-5 0.0 6.128071027800073E-4 1.57130026353848E-4 26-27 0.0 3.142600527076961E-5 0.0 0.0012570402108307843 1.57130026353848E-4 28-29 0.0 3.142600527076961E-5 0.0 0.0040696676825646635 1.57130026353848E-4 30-31 0.0 3.142600527076961E-5 0.0 0.013418904250618622 1.57130026353848E-4 32-33 0.0 3.142600527076961E-5 0.0 0.03337441759755732 1.57130026353848E-4 34-35 0.0 3.142600527076961E-5 0.0 0.06351195665222537 1.57130026353848E-4 36-37 0.0 3.142600527076961E-5 0.0 0.11369928706964443 1.57130026353848E-4 38-39 0.0 3.142600527076961E-5 0.0 0.1913843720989869 1.57130026353848E-4 40-41 0.0 3.142600527076961E-5 0.0 0.27643885536432483 1.57130026353848E-4 42-43 0.0 3.142600527076961E-5 0.0 0.36407027106186585 1.7284302898923283E-4 44-45 0.0 3.142600527076961E-5 0.0 0.47338563039623793 2.0426903426000242E-4 46-47 0.0 3.142600527076961E-5 0.0 0.6016194449036133 2.1998203689538724E-4 48-49 0.0 3.142600527076961E-5 0.0 0.7384325588499088 2.8283404743692644E-4 50-51 0.0 3.142600527076961E-5 0.0 0.893771302903323 2.8283404743692644E-4 52-53 0.0 3.142600527076961E-5 0.0 1.0417406487207415 2.8283404743692644E-4 54-55 0.0 3.142600527076961E-5 0.0 1.1979750339243727 3.14260052707696E-4 56-57 0.0 3.142600527076961E-5 0.0 1.3821157118084473 3.14260052707696E-4 58-59 0.0 3.142600527076961E-5 0.0 1.5779311506506124 3.14260052707696E-4 60-61 0.0 3.142600527076961E-5 0.0 1.7993430707858198 3.14260052707696E-4 62-63 0.0 3.142600527076961E-5 0.0 2.010133001139507 3.4568605797846567E-4 64-65 0.0 3.142600527076961E-5 0.0 2.2254482762521848 3.4568605797846567E-4 66-67 0.0 3.142600527076961E-5 0.0 2.4814916541957803 3.4568605797846567E-4 68-69 0.0 3.142600527076961E-5 0.0 2.7685367863389896 3.4568605797846567E-4 70-71 0.0 3.142600527076961E-5 0.0 3.0643340609501086 3.4568605797846567E-4 72-73 0.0 3.142600527076961E-5 0.0 3.374555871980511 3.4568605797846567E-4 74-75 0.0 3.142600527076961E-5 0.0 3.678618185977842 3.4568605797846567E-4 76-77 0.0 3.142600527076961E-5 0.0 4.00379877551713 3.4568605797846567E-4 78-79 3.142600527076961E-5 3.142600527076961E-5 0.0 4.376134085965209 3.4568605797846567E-4 80-81 6.285201054153921E-5 3.142600527076961E-5 0.0 4.767670685633727 3.4568605797846567E-4 82-83 6.285201054153921E-5 3.142600527076961E-5 0.0 5.172610476550229 3.7711206324923525E-4 84-85 6.285201054153921E-5 3.142600527076961E-5 0.0 5.579262984753988 3.9282506588462005E-4 86-87 6.285201054153921E-5 3.142600527076961E-5 0.0 5.994667635425656 4.242510711553897E-4 88-89 6.285201054153921E-5 3.142600527076961E-5 0.0 6.424339692490253 4.399640737907745E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5785 0.0 49.558407 1 GTATCAA 10540 0.0 43.946552 1 TATCAAC 14125 0.0 32.604183 2 ATCAACG 14145 0.0 32.558083 3 AGGTAAG 1900 0.0 31.767872 2 TCAACGC 14520 0.0 31.61903 4 CAACGCA 14555 0.0 31.444542 5 AACGCAG 15110 0.0 30.250975 6 TAAGGTG 2480 0.0 28.937197 5 AAGGTAA 2090 0.0 27.845804 1 TAAGGTA 1035 0.0 26.63334 4 ACGCAGA 17205 0.0 26.3143 7 CGCAGAG 17380 0.0 25.994675 8 GTGTAAG 1180 0.0 25.468582 1 GTAAGGT 2605 0.0 24.994867 4 GGTAAGG 2675 0.0 24.518467 3 TACATGG 13700 0.0 23.659294 2 GTACATG 14000 0.0 23.544867 1 TGGTATC 2890 0.0 23.35224 2 GCAGAGT 19150 0.0 22.970036 9 >>END_MODULE