FastQCFastQC Report
Thu 26 May 2016
SRR938372_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938372_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3182078
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90140.28327401151071724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75500.23726633979431053No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA63770.20040363561169777No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA62640.1968524970161008No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA60320.18956166379328226No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC59550.187141861387433No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA56550.1777140598062021No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG54510.17130315473096513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50730.1594241247386142No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA46150.14503101432460172No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA43860.13783445911759548No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA41590.13070075592113078No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG39780.1250126489671215No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA39780.1250126489671215No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT37410.11756468571794908No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT35840.11263080289043827No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG35060.11017957447931823No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT33770.10612561979938895No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG33210.10436576350422586No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA31990.10053179086119196No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57700.052.3738061
GTATCAA100250.042.2778931
AGGTAAG19050.033.16452
TATCAAC132600.031.8474772
TAAGGTG23950.031.731985
ATCAACG133500.031.4879743
TCAACGC137400.030.628784
CAACGCA138800.030.3540635
AACGCAG144100.029.2371836
TGGTATC26350.028.8440462
AAGGTAA20900.027.9635091
GTGTAAG11200.027.5758041
GTAAGGT26500.026.8861164
TAAGGTA11150.026.4118924
GTGGTAT31950.026.0255581
ACGCAGA164650.025.4438427
CGCAGAG167450.024.9049228
GGTAAGG28050.024.553753
TACATGG130350.024.3434562
GTACATG132750.024.0529081
Produced by FastQC (version 0.11.5)