##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938372_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3182078 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85081195369818 31.0 31.0 34.0 30.0 34.0 2 32.10413132550491 33.0 31.0 34.0 30.0 34.0 3 32.15692481453943 34.0 31.0 34.0 30.0 34.0 4 35.80613957294573 37.0 35.0 37.0 35.0 37.0 5 35.6950656143564 37.0 35.0 37.0 33.0 37.0 6 35.732710511810204 37.0 35.0 37.0 33.0 37.0 7 35.65121470938173 37.0 35.0 37.0 33.0 37.0 8 35.615375550190784 37.0 35.0 37.0 33.0 37.0 9 37.17567702614455 39.0 37.0 39.0 33.0 39.0 10-11 37.10191469222313 39.0 37.0 39.0 33.0 39.0 12-13 36.98096338304718 39.0 37.0 39.0 33.0 39.0 14-15 38.01187321618138 40.0 37.0 41.0 33.0 41.0 16-17 38.096218885897834 40.0 37.0 41.0 33.0 41.0 18-19 38.231639513550576 40.0 38.0 41.0 33.0 41.0 20-21 38.173547285767356 40.0 37.5 41.0 33.0 41.0 22-23 38.05089048728535 40.0 37.0 41.0 33.0 41.0 24-25 37.913324249122745 40.0 37.0 41.0 32.5 41.0 26-27 37.689889437028256 40.0 37.0 41.0 32.0 41.0 28-29 37.54959259326767 39.5 37.0 41.0 32.0 41.0 30-31 37.280418016151714 39.0 36.0 41.0 31.0 41.0 32-33 37.17420424640754 39.0 36.0 41.0 31.0 41.0 34-35 37.044552176282295 39.0 36.0 41.0 31.0 41.0 36-37 36.90351697852787 39.0 35.5 41.0 30.5 41.0 38-39 36.67251651279447 39.0 35.0 41.0 30.0 41.0 40-41 36.43310377684017 39.0 35.0 40.0 30.0 41.0 42-43 36.14939152968594 38.5 35.0 40.0 30.0 41.0 44-45 35.838774222379215 38.0 35.0 40.0 29.0 41.0 46-47 35.40264066437089 38.0 34.0 40.0 27.5 41.0 48-49 34.95121536932784 38.0 34.0 40.0 26.0 41.0 50-51 34.52284670583185 38.0 33.0 40.0 25.0 41.0 52-53 33.897140799188456 37.0 33.0 40.0 23.5 41.0 54-55 33.44483856146832 37.0 32.0 39.5 22.5 41.0 56-57 33.01730394415222 36.0 31.5 39.0 21.5 40.0 58-59 32.447416593810715 35.5 31.0 39.0 19.5 40.0 60-61 31.931502464741595 35.0 31.0 38.5 18.0 40.0 62-63 31.40834951248838 35.0 30.0 38.0 14.0 40.0 64-65 30.73519740873731 34.5 29.0 37.5 9.0 40.0 66-67 30.081039653961973 34.0 28.5 37.0 7.5 39.0 68-69 29.349836019104497 34.0 27.5 36.0 4.0 39.0 70-71 28.568876690011997 33.0 26.0 35.5 2.0 38.5 72-73 28.011289792393526 33.0 26.0 35.0 2.0 38.0 74-75 27.32996677014203 32.0 25.0 35.0 2.0 37.0 76-77 26.201883171939848 30.5 24.0 34.0 2.0 36.0 78-79 26.70866616091749 32.0 25.0 34.5 2.0 36.0 80-81 26.375995497281963 32.0 24.0 35.0 2.0 36.0 82-83 25.738193092689748 31.5 22.0 34.0 2.0 35.0 84-85 25.007240865874437 31.0 19.5 34.0 2.0 35.0 86-87 24.161157268929298 30.5 15.5 34.0 2.0 35.0 88-89 23.205276394858956 30.0 7.0 34.0 2.0 35.0 90-91 22.383629659612367 29.0 2.0 34.0 2.0 35.0 92-93 21.354239116702985 29.0 2.0 33.5 2.0 35.0 94-95 20.225998545604476 27.0 2.0 33.0 2.0 34.5 96-97 18.888841976846578 25.5 2.0 33.0 2.0 34.0 98-99 17.141476733128478 21.0 2.0 32.0 2.0 34.0 100-101 12.848731080759176 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34096969850511627 1101 2 0.3027035849936013 1101 3 0.336813112497822 1101 4 0.27461776225192125 1101 5 0.1583950095461475 1101 6 0.19234518564299918 1101 7 0.29846038468780023 1101 8 0.28910684171677303 1101 9 0.3313156039229739 1101 10-11 0.31587623190580416 1101 12-13 0.36491695164183113 1101 14-15 0.45162598442068 1101 16-17 0.4086199233068868 1101 18-19 0.39209930721481356 1101 20-21 0.49208176450013497 1101 22-23 0.4359874635340617 1101 24-25 0.464043881211488 1101 26-27 0.4993613277113056 1101 28-29 0.5505227268646635 1101 30-31 0.6992531895854555 1101 32-33 0.5712024576563124 1101 34-35 0.5034819975720026 1101 36-37 0.5967349823894352 1101 38-39 0.65342879226246 1101 40-41 0.6166488803536296 1101 42-43 0.7448585155418783 1101 44-45 0.8383237893326623 1101 46-47 0.697376518410401 1101 48-49 0.8474711092332115 1101 50-51 0.8740689912783708 1101 52-53 0.9175719424646189 1101 54-55 0.9354580033093782 1101 56-57 1.0538579821363996 1101 58-59 1.071245383966108 1101 60-61 1.0512658058721236 1101 62-63 1.1476900401922734 1101 64-65 1.2772432810763803 1101 66-67 1.4405431634409744 1101 68-69 1.6387175208216611 1101 70-71 1.7999943485318255 1101 72-73 1.8660383415068367 1101 74-75 1.9134242618573936 1101 76-77 1.851615062250815 1101 78-79 1.9413936017210922 1101 80-81 1.919941236295351 1101 82-83 2.222698132589006 1101 84-85 2.412110181876624 1101 86-87 2.3346542436791644 1101 88-89 2.232676725263296 1101 90-91 2.5354134811070566 1101 92-93 2.776133653347781 1101 94-95 2.5994773103169457 1101 96-97 2.7747609577121715 1101 98-99 2.6013250842668008 1101 100-101 2.254865028552633 1102 1 0.23106332874145963 1102 2 0.20547722995343065 1102 3 0.16863885570909076 1102 4 0.17074740316589043 1102 5 0.17586089962109952 1102 6 0.18094908365501539 1102 7 0.24166336155398938 1102 8 0.1695440921366682 1102 9 0.17543038525136723 1102 10-11 0.24275946166952167 1102 12-13 0.27680752768276307 1102 14-15 0.3928685724136116 1102 16-17 0.28335627701699195 1102 18-19 0.29959727345596576 1102 20-21 0.31591989276149945 1102 22-23 0.30050522296591 1102 24-25 0.2175847249662226 1102 26-27 0.2861938913610018 1102 28-29 0.2449656006664469 1102 30-31 0.16961901872497265 1102 32-33 0.2663804540867929 1102 34-35 0.46582284627580606 1102 36-37 0.44117819643985 1102 38-39 0.46063018542593426 1102 40-41 0.49812283741425034 1102 42-43 0.5692532286968159 1102 44-45 0.347044361692717 1102 46-47 0.4428147431786513 1102 48-49 0.530166991915479 1102 50-51 0.6392130556410365 1102 52-53 0.6779744256609774 1102 54-55 0.5919757132505126 1102 56-57 0.7388603174792578 1102 58-59 0.8438949000397642 1102 60-61 0.8427555071647568 1102 62-63 1.0686807204730115 1102 64-65 1.1051439161040406 1102 66-67 1.03701490888551 1102 68-69 1.2992621660815367 1102 70-71 1.532908578038807 1102 72-73 1.5659154808200881 1102 74-75 1.4358418211611799 1102 76-77 1.3578182896637223 1102 78-79 1.4754094944252358 1102 80-81 1.63195986954754 1102 82-83 1.869708244159625 1102 84-85 1.9324298299976768 1102 86-87 1.743562076511921 1102 88-89 1.7270111525475258 1102 90-91 1.870256301706327 1102 92-93 2.0078458153803673 1102 94-95 1.8216065862369284 1102 96-97 1.9004301185043921 1102 98-99 1.9785038663622991 1102 100-101 1.651728307374011 1103 1 0.1386344434340252 1103 2 0.18203877730186235 1103 3 0.18532590266378435 1103 4 0.06557106970306847 1103 5 0.15088917409831026 1103 6 0.1710856852948126 1103 7 0.1815001374620664 1103 8 0.17194121063662493 1103 9 0.1828080141049 1103 10-11 0.2799889300196412 1103 12-13 0.2616014011072991 1103 14-15 0.3430452310875225 1103 16-17 0.2585619125296148 1103 18-19 0.26445688791734057 1103 20-21 0.21057139219706755 1103 22-23 0.3561383333968493 1103 24-25 0.35079130270566594 1103 26-27 0.3053897263926899 1103 28-29 0.3616126779395543 1103 30-31 0.38139382014258416 1103 32-33 0.33403575064211566 1103 34-35 0.47360259184623743 1103 36-37 0.46533677621058445 1103 38-39 0.4161481557876954 1103 40-41 0.5073201137625816 1103 42-43 0.3334423771289323 1103 44-45 0.49772132604144304 1103 46-47 0.6180023533800494 1103 48-49 0.7152920140339702 1103 50-51 0.7103901934553463 1103 52-53 0.7863058968509904 1103 54-55 0.8648963956248181 1103 56-57 1.043723161623113 1103 58-59 0.9483593104379722 1103 60-61 0.8911610816250466 1103 62-63 0.9702142412365333 1103 64-65 1.0652543273325747 1103 66-67 1.0507984287932075 1103 68-69 0.8825285389840261 1103 70-71 1.0860838363573357 1103 72-73 1.2265715266003667 1103 74-75 1.1830083236877336 1103 76-77 1.258606421902691 1103 78-79 1.2433697339175218 1103 80-81 1.499396395249665 1103 82-83 1.5182537479186742 1103 84-85 1.5902809744514848 1103 86-87 1.6783106859836963 1103 88-89 1.610435255776487 1103 90-91 1.809363743067113 1103 92-93 2.1208208404676547 1103 94-95 1.9162960722157294 1103 96-97 2.0825526927454803 1103 98-99 2.1743743112380756 1103 100-101 1.9353025152902497 1104 1 0.16101517419410882 1104 2 0.19063865934663937 1104 3 0.22549451975948642 1104 4 0.18908798781018277 1104 5 0.09533808403786992 1104 6 0.10381713604155607 1104 7 0.18417414209493188 1104 8 0.12925603895818227 1104 9 0.13950065010651258 1104 10-11 0.1731374050861305 1104 12-13 0.17197527551309832 1104 14-15 0.3625341402861437 1104 16-17 0.35399599338683174 1104 18-19 0.27977932478103185 1104 20-21 0.2593144214764962 1104 22-23 0.32555382654793874 1104 24-25 0.32403521367228905 1104 26-27 0.4075437158494921 1104 28-29 0.2843589508223161 1104 30-31 0.3983822174631584 1104 32-33 0.4773602819884104 1104 34-35 0.5035382787932008 1104 36-37 0.5124637924229773 1104 38-39 0.5243791879356223 1104 40-41 0.5758345361540975 1104 42-43 0.6639392941180304 1104 44-45 0.6080212055392238 1104 46-47 0.6839755782713155 1104 48-49 0.736740117868969 1104 50-51 0.7362081977362607 1104 52-53 0.8893111419884789 1104 54-55 1.0056481402275992 1104 56-57 1.0863372161570695 1104 58-59 1.136903264637212 1104 60-61 0.8912518800258091 1104 62-63 1.0319096871922966 1104 64-65 1.2793331403444341 1104 66-67 1.266960147195114 1104 68-69 1.3716567122558239 1104 70-71 1.398286612175255 1104 72-73 1.5850859522996572 1104 74-75 1.400623808834009 1104 76-77 1.4571659854882029 1104 78-79 1.672585186118365 1104 80-81 1.9742480498479154 1104 82-83 1.8878125007687352 1104 84-85 1.9741772744083796 1104 86-87 1.9932379099904622 1104 88-89 2.231028101524064 1104 90-91 2.46196972332433 1104 92-93 2.5053477675686118 1104 94-95 2.16511577570024 1104 96-97 2.262322114424169 1104 98-99 2.2428604521604996 1104 100-101 1.955805918824474 1105 1 0.1383749531232219 1105 2 0.21702232091571716 1105 3 0.16099144218953398 1105 4 0.10862424686573036 1105 5 0.014592371270055082 1105 6 0.062198592877308556 1105 7 0.1803955329256226 1105 8 0.15126633622059416 1105 9 0.20807644652908408 1105 10-11 0.2870194755062627 1105 12-13 0.1609122386267643 1105 14-15 0.2608199632346029 1105 16-17 0.13808774066912122 1105 18-19 0.2664085383690846 1105 20-21 0.2659518078902465 1105 22-23 0.2813875777037609 1105 24-25 0.256928409530353 1105 26-27 0.2779000118352499 1105 28-29 0.37351059844343837 1105 30-31 0.3541428302321208 1105 32-33 0.4431984307842498 1105 34-35 0.4631067560862192 1105 36-37 0.5163723389391492 1105 38-39 0.5656717206308173 1105 40-41 0.537127212013786 1105 42-43 0.5811138057325564 1105 44-45 0.5551860348762503 1105 46-47 0.6492619212803916 1105 48-49 0.739573996939157 1105 50-51 0.8820382048291293 1105 52-53 0.8342536536602765 1105 54-55 0.7714983957606805 1105 56-57 0.9506902511044544 1105 58-59 1.1047886658106023 1105 60-61 1.0290537820595809 1105 62-63 1.1615947844212258 1105 64-65 1.3576976539726004 1105 66-67 1.3250877360216862 1105 68-69 1.541292019360796 1105 70-71 1.6981810750391624 1105 72-73 1.7490769989161947 1105 74-75 1.5927308074929307 1105 76-77 1.5479543768389483 1105 78-79 1.6705701457537572 1105 80-81 1.8328342007986969 1105 82-83 2.0372206867282223 1105 84-85 2.0945353599504166 1105 86-87 1.9874900108517188 1105 88-89 1.9168083184929792 1105 90-91 1.9634955145649684 1105 92-93 2.195765891945573 1105 94-95 2.0482364319414152 1105 96-97 2.1734449691741347 1105 98-99 1.9946973927724443 1105 100-101 1.503347893255917 1106 1 0.017262902712825934 1106 2 0.03523823184615793 1106 3 0.02142156132066475 1106 4 0.01949503725609958 1106 5 0.08669967921832722 1106 6 -0.05745834304849495 1106 7 0.024507025751972833 1106 8 0.0022837772096195863 1106 9 -0.1432878357163645 1106 10-11 0.10449508869881896 1106 12-13 0.11790632104128917 1106 14-15 0.04074408983615996 1106 16-17 0.09740269171564364 1106 18-19 0.09924769036170034 1106 20-21 0.06390928161484766 1106 22-23 0.20811608823490246 1106 24-25 0.20720885032152125 1106 26-27 -0.06720988618082657 1106 28-29 0.10259625054697352 1106 30-31 0.2132897566250307 1106 32-33 0.15591164672515845 1106 34-35 0.15533205601247602 1106 36-37 0.1618596554010665 1106 38-39 0.1656242060766715 1106 40-41 0.27510827813845395 1106 42-43 0.23450397666504585 1106 44-45 0.1653817288885122 1106 46-47 -0.3597857791351018 1106 48-49 -0.030922612590131848 1106 50-51 0.23145364091381282 1106 52-53 0.004631551748303764 1106 54-55 -0.063572441394804 1106 56-57 0.16216988729585324 1106 58-59 0.4203879787231699 1106 60-61 0.3374596612736873 1106 62-63 0.3516102828593901 1106 64-65 0.5309943246562696 1106 66-67 0.4988241786354948 1106 68-69 0.47559707778721005 1106 70-71 0.17315124729066156 1106 72-73 0.46445043033635613 1106 74-75 0.5716746653749532 1106 76-77 0.6014127468948871 1106 78-79 0.8569763367352863 1106 80-81 0.751189357851537 1106 82-83 1.1658764595217157 1106 84-85 1.1668424688471823 1106 86-87 0.9592985916504446 1106 88-89 1.1002931887797445 1106 90-91 1.2462213510909166 1106 92-93 1.152187251611263 1106 94-95 1.2291044044339365 1106 96-97 1.1441487241729043 1106 98-99 1.5050201919642454 1106 100-101 1.1920084025160094 1107 1 0.06552794075575008 1107 2 0.1542193184293481 1107 3 0.10007203273445242 1107 4 0.09786857439485885 1107 5 0.09301230218965628 1107 6 0.0934327370520478 1107 7 0.02558291825370418 1107 8 0.07998630316865274 1107 9 0.09200735233736168 1107 10-11 0.10254716599020952 1107 12-13 0.19067407156160243 1107 14-15 0.275739881799943 1107 16-17 0.19386964725831035 1107 18-19 0.1630446469279363 1107 20-21 0.2119425933393515 1107 22-23 0.25347672831133394 1107 24-25 0.19042703131257355 1107 26-27 0.2605259060104217 1107 28-29 0.23723094751149887 1107 30-31 0.21928535524661896 1107 32-33 0.2627610786402741 1107 34-35 0.34609371056681937 1107 36-37 0.4032437012572885 1107 38-39 0.3820284391336415 1107 40-41 0.4991100068452212 1107 42-43 0.44769560153567056 1107 44-45 0.37527402590763614 1107 46-47 0.42609938696860183 1107 48-49 0.5312005122748147 1107 50-51 0.5431303727481733 1107 52-53 0.6598796552887407 1107 54-55 0.6258756234771923 1107 56-57 0.6300233339049726 1107 58-59 0.7158504077836412 1107 60-61 0.7284797688629432 1107 62-63 0.9916596988522031 1107 64-65 0.9927805222974939 1107 66-67 1.0359888152090164 1107 68-69 1.1340953206477629 1107 70-71 1.0579704723296146 1107 72-73 1.2512713158436526 1107 74-75 1.325397355788592 1107 76-77 1.314937887067984 1107 78-79 1.4463246029106358 1107 80-81 1.5659717910063158 1107 82-83 1.6580532084156836 1107 84-85 1.962209932766111 1107 86-87 2.178966896039313 1107 88-89 2.249921573689953 1107 90-91 2.2255067241845055 1107 92-93 2.398117682536917 1107 94-95 2.34757142821503 1107 96-97 2.247853501999966 1107 98-99 2.4110246047157347 1107 100-101 2.3760971920800777 1108 1 0.037251110259997944 1108 2 0.06719577901597518 1108 3 0.022305995282927427 1108 4 0.0364172070674158 1108 5 0.00517452198562296 1108 6 0.04869338651125332 1108 7 0.01977039053184626 1108 8 0.06922560110898957 1108 9 0.014082447302506296 1108 10-11 0.04256505725227555 1108 12-13 0.047249874500401745 1108 14-15 0.1251878791443346 1108 16-17 0.022461653979789276 1108 18-19 0.048618759544091006 1108 20-21 0.03914513067145009 1108 22-23 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10569.0 13 14394.0 14 19119.0 15 25656.0 16 32452.0 17 41088.0 18 40714.0 19 38368.0 20 39911.0 21 42660.0 22 46705.0 23 51552.0 24 58107.0 25 65980.0 26 76461.0 27 89659.0 28 105444.0 29 123786.0 30 144266.0 31 168978.0 32 199153.0 33 239900.0 34 294493.0 35 358949.0 36 405634.0 37 332268.0 38 96007.0 39 1841.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.43769960820211 18.134672353352414 14.832052487841189 23.595575550604295 2 16.815213435274195 19.861338888410458 37.51547122085769 25.807976455457656 3 19.91077368754009 22.820276798927473 29.138714043684814 28.13023546984762 4 14.04896423029228 16.152432467085973 34.365593803797395 35.433009498824354 5 14.726801964382293 36.774878231741674 33.005632498855306 15.492687305020727 6 34.692047146550145 34.468042580980104 15.958596866575867 14.881313405893884 7 30.805027406619196 31.8630467260702 18.782977664281013 18.548948203029592 8 28.457522699017872 33.206812156592115 18.641352374990415 19.6943127693996 9 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25.22628776811791 27.52994870784401 24.439723192373002 34-35 24.029024907676593 25.401178596684215 27.181645320971164 23.388151174668028 36-37 24.15029731717661 24.638443363132357 27.293014433038937 23.918244886652097 38-39 23.378154461599582 25.10139284730979 27.322339364940074 24.198113326150555 40-41 23.8550940592449 24.90265005265281 27.617825298272948 23.62443058982934 42-43 23.527013101213452 24.94552815455076 27.269785937354158 24.257672806881633 44-45 23.452251936290764 25.24406825095956 27.575756575830585 23.72792323691909 46-47 24.206430016127836 25.71016531346203 26.481375680530572 23.60202898987956 48-49 23.687021499787246 25.78722143203278 26.35026545546652 24.175491612713454 50-51 23.94715025841604 25.87604703593061 26.583226432538737 23.593576273114614 52-53 24.52450882725062 25.25005672393951 26.61268831248008 23.612746136329783 54-55 23.63688130837773 25.838540098639946 26.67616884312704 23.848409749855286 56-57 23.64363789951095 26.067132860979527 26.42680349130348 23.862425748206046 58-59 23.58265573628302 25.295294458526783 26.478577834987078 24.64347197020312 60-61 22.93736985705567 25.822308566917595 26.960259930774793 24.280061645251937 62-63 23.34036123564539 25.84617661792074 27.11388595754095 23.69957618889292 64-65 22.981532959080624 25.924628728837117 26.724515720797864 24.369322591284394 66-67 22.829457754122956 25.961027786385056 26.81280251610434 24.396711943387654 68-69 23.7717531066102 25.245396396751712 26.990971687865557 23.991878808772533 70-71 23.47416851857584 25.573238104112207 26.32644124049186 24.626152136820092 72-73 23.308518777318103 25.75714460129026 26.29729605299443 24.63704056839721 74-75 23.239090933660332 26.226839819765573 26.159415325457136 24.374653921116956 76-77 23.408147129014438 25.657353465251326 26.583352136559817 24.35114726917442 78-79 23.44089302650658 25.933085235497057 26.596378215744554 24.029643522251813 80-81 23.701608982572054 25.936755693536885 26.211545115401186 24.150090208489875 82-83 23.51858538680967 25.994510445019124 26.288362539412574 24.19854162875863 84-85 23.227199214553885 26.14160281040867 26.330814491782544 24.3003834832549 86-87 23.707385707412108 26.131297763662015 25.84617323440426 24.31514329452162 88-89 23.269275611722907 26.38775667975455 25.52440574995333 24.818561958569212 90-91 23.08195130413358 26.19775514796187 25.908082121006803 24.812211426897747 92-93 23.186750455248045 25.688355942139935 26.1666786871949 24.95821491541712 94-95 22.954900675706572 25.32970216146595 26.47899885499296 25.236398307834524 96-97 23.276683750896034 24.94172831141421 26.38591083748131 25.395677100208445 98-99 22.786807865803414 25.53160230516034 25.91311400914748 25.768475819888764 100-101 23.10868027392172 24.648083625883345 26.39585717127524 25.847378928919696 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 446.0 1 332.0 2 296.0 3 413.0 4 373.0 5 367.5 6 671.0 7 1434.5 8 2272.0 9 2602.0 10 2243.5 11 1853.5 12 1915.5 13 2053.5 14 2245.0 15 2531.5 16 2807.5 17 3045.0 18 3366.5 19 3777.0 20 4284.0 21 4910.0 22 5826.0 23 6995.0 24 8276.0 25 10139.0 26 12731.5 27 15761.5 28 18523.5 29 21331.0 30 24794.0 31 31922.0 32 43924.5 33 46266.5 34 41484.5 35 42702.5 36 46868.5 37 52453.0 38 59496.5 39 64622.5 40 69059.0 41 76678.0 42 82029.0 43 87330.5 44 93761.5 45 101349.0 46 107078.5 47 112340.0 48 115453.5 49 114671.0 50 116932.5 51 118802.0 52 122044.5 53 130584.0 54 138697.0 55 134636.5 56 126590.0 57 120597.0 58 115105.5 59 104774.0 60 85036.0 61 69703.0 62 59599.5 63 45418.0 64 32230.0 65 24582.0 66 18732.0 67 14592.5 68 11481.0 69 9005.0 70 7127.0 71 4918.0 72 3510.5 73 2864.5 74 2112.0 75 1502.0 76 1095.5 77 750.5 78 484.0 79 287.0 80 170.5 81 93.5 82 63.5 83 32.5 84 15.5 85 11.0 86 6.5 87 3.5 88 1.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026586400459071083 2 0.011439065918560136 3 0.0017912823004338674 4 0.0 5 2.8283404743692644E-4 6 0.0 7 0.0 8 6.28520105415392E-5 9 3.14260052707696E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0018069953030692522 26-27 0.00831217839411856 28-29 2.1998203689538724E-4 30-31 0.0 32-33 3.928250658846201E-4 34-35 0.01030772972881243 36-37 0.0012099012029246298 38-39 0.012381846076683222 40-41 0.012146151037152452 42-43 0.004996734838052367 44-45 8.642151449461641E-4 46-47 6.28520105415392E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.0999101844769362E-4 66-67 5.028160843323136E-4 68-69 0.001021345171300012 70-71 4.713900790615441E-5 72-73 2.3569503953077206E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.57130026353848E-4 82-83 0.001099910184476936 84-85 0.006033793011987764 86-87 0.004242510711553897 88-89 0.0 90-91 0.0013198922213723233 92-93 6.756591133215465E-4 94-95 4.713900790615441E-5 96-97 3.14260052707696E-5 98-99 0.0 100-101 3.14260052707696E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3182078.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.601089416398295 #Duplication Level Percentage of deduplicated Percentage of total 1 69.04130085429139 25.960281268466673 2 14.896496070161525 11.202489614503385 3 5.831946795627453 6.578626588021962 4 2.917420671403225 4.387927821227257 5 1.7016500002295356 3.1991946907022473 6 1.111378203764962 2.5073418729121477 7 0.7737636841914607 2.0366050233511417 8 0.5447457559849067 1.6386427103993568 9 0.4204390016021022 1.4228068044043685 >10 2.3993404820915933 16.826323861859848 >50 0.2126686544525123 5.5354371527532304 >100 0.13123519327661795 9.308328662394523 >500 0.010652658958172162 2.757908030353221 >1k 0.006207061125167122 4.828633551314524 >5k 7.549128395473527E-4 1.809452347336122 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9014 0.28327401151071724 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7550 0.23726633979431053 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6377 0.20040363561169777 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6264 0.1968524970161008 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6032 0.18956166379328226 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5955 0.187141861387433 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5655 0.1777140598062021 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5451 0.17130315473096513 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5073 0.1594241247386142 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4615 0.14503101432460172 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4386 0.13783445911759548 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4159 0.13070075592113078 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3978 0.1250126489671215 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3978 0.1250126489671215 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3741 0.11756468571794908 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3584 0.11263080289043827 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3506 0.11017957447931823 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3377 0.10612561979938895 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3321 0.10436576350422586 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3199 0.10053179086119196 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.142600527076961E-5 2 0.0 0.0 0.0 0.0 3.142600527076961E-5 3 0.0 0.0 0.0 0.0 3.142600527076961E-5 4 0.0 0.0 0.0 0.0 3.142600527076961E-5 5 0.0 0.0 0.0 0.0 3.142600527076961E-5 6 0.0 0.0 0.0 0.0 3.142600527076961E-5 7 0.0 0.0 0.0 0.0 3.142600527076961E-5 8 0.0 0.0 0.0 0.0 3.142600527076961E-5 9 0.0 0.0 0.0 0.0 3.142600527076961E-5 10-11 0.0 0.0 0.0 0.0 3.142600527076961E-5 12-13 0.0 0.0 0.0 0.0 3.142600527076961E-5 14-15 0.0 0.0 0.0 0.0 3.142600527076961E-5 16-17 0.0 0.0 0.0 0.0 3.142600527076961E-5 18-19 0.0 0.0 0.0 0.0 3.142600527076961E-5 20-21 0.0 0.0 0.0 0.0 9.427801581230881E-5 22-23 0.0 0.0 0.0 2.1998203689538724E-4 9.427801581230881E-5 24-25 0.0 0.0 0.0 5.813810975092376E-4 9.427801581230881E-5 26-27 0.0 0.0 0.0 0.0012256142055600146 9.427801581230881E-5 28-29 0.0 0.0 0.0 0.004053954679929279 9.427801581230881E-5 30-31 0.0 0.0 0.0 0.013544608271701699 9.427801581230881E-5 32-33 0.0 0.0 0.0 0.03356297362918194 9.427801581230881E-5 34-35 0.0 0.0 0.0 0.06387335571283922 1.57130026353848E-4 36-37 0.0 0.0 0.0 0.11445351119614289 1.57130026353848E-4 38-39 0.0 0.0 0.0 0.19245285627819306 1.57130026353848E-4 40-41 0.0 0.0 0.0 0.27753876554880175 1.57130026353848E-4 42-43 0.0 0.0 0.0 0.3660658223965597 1.57130026353848E-4 44-45 0.0 0.0 0.0 0.4756640157783687 1.57130026353848E-4 46-47 0.0 0.0 0.0 0.6045263503911595 1.57130026353848E-4 48-49 0.0 0.0 0.0 0.7415280203690796 1.57130026353848E-4 50-51 0.0 0.0 0.0 0.8970081814462122 1.57130026353848E-4 52-53 0.0 0.0 0.0 1.044820397237277 1.57130026353848E-4 54-55 0.0 0.0 0.0 1.2020918406148433 1.57130026353848E-4 56-57 0.0 0.0 0.0 1.3859968234593874 1.57130026353848E-4 58-59 0.0 0.0 0.0 1.582189374364802 1.57130026353848E-4 60-61 0.0 0.0 0.0 1.8037269985210922 1.57130026353848E-4 62-63 0.0 0.0 0.0 2.014878327935393 1.57130026353848E-4 64-65 0.0 0.0 0.0 2.2316391992905267 1.8855603162461763E-4 66-67 0.0 0.0 0.0 2.489442433529285 1.8855603162461763E-4 68-69 0.0 0.0 0.0 2.7780745789386683 1.8855603162461763E-4 70-71 0.0 0.0 0.0 3.0793714044721723 1.8855603162461763E-4 72-73 0.0 0.0 0.0 3.396035546583082 1.8855603162461763E-4 74-75 0.0 0.0 0.0 3.7087242990272395 2.0426903426000242E-4 76-77 0.0 0.0 0.0 4.044778286390214 2.5140804216615685E-4 78-79 0.0 0.0 0.0 4.431710976286565 2.5140804216615685E-4 80-81 0.0 0.0 0.0 4.842370300162347 2.5140804216615685E-4 82-83 0.0 0.0 0.0 5.269009747718314 2.5140804216615685E-4 84-85 0.0 0.0 0.0 5.7015887102704585 2.5140804216615685E-4 86-87 0.0 0.0 0.0 6.143909734456541 2.5140804216615685E-4 88-89 0.0 0.0 0.0 6.610853033772271 2.5140804216615685E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5770 0.0 52.373806 1 GTATCAA 10025 0.0 42.277893 1 AGGTAAG 1905 0.0 33.1645 2 TATCAAC 13260 0.0 31.847477 2 TAAGGTG 2395 0.0 31.73198 5 ATCAACG 13350 0.0 31.487974 3 TCAACGC 13740 0.0 30.62878 4 CAACGCA 13880 0.0 30.354063 5 AACGCAG 14410 0.0 29.237183 6 TGGTATC 2635 0.0 28.844046 2 AAGGTAA 2090 0.0 27.963509 1 GTGTAAG 1120 0.0 27.575804 1 GTAAGGT 2650 0.0 26.886116 4 TAAGGTA 1115 0.0 26.411892 4 GTGGTAT 3195 0.0 26.025558 1 ACGCAGA 16465 0.0 25.443842 7 CGCAGAG 16745 0.0 24.904922 8 GGTAAGG 2805 0.0 24.55375 3 TACATGG 13035 0.0 24.343456 2 GTACATG 13275 0.0 24.052908 1 >>END_MODULE